Proteins and nucleic acids from meningitis/sepsis-associated escherichia coli

ABSTRACT

Disclosed herein are various open reading frames from a strain of  E. coli  responsible for neonatal meningitis (MNEC), and a subset of these that is of particular interest for preparing compositions for immunising against MNEC infections.

All documents cited herein are incorporated by reference in theirentirety.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.11/884,812, claiming an international filing date of Feb. 17, 2006,which is a U.S. National Phase of PCT/US2006/005913, filed Feb. 17,2006, which claims the benefit of U.S. Provisional Application No.60/654,632, filed Feb. 18, 2005 and U.S. Provisional Application No.60/712,720, filed Aug. 29, 2005, all of which are hereby incorporated byreference in the present disclosure in their entirety.

TECHNICAL FIELD

This invention is in the field of Escherichia coli biology, and inparticular relates to immunogens for use in immunising againstextraintestinal pathogenic E. coli (ExPEC) strains.

SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE

The content of the following submission on ASCII text file isincorporated herein by reference in its entirety: a computer readableform (CRF) of the Sequence Listing (file name: 529552001710SeqList.txt,date recorded: May 30, 2014, size: 27.1 MB).

BACKGROUND ART

Few microorganisms are as versatile as E. coli. As well as being animportant member of the normal intestinal microflora of mammals, it hasbeen widely exploited as a host in recombinant DNA technology. Inaddition, however, E. coli can also be a deadly pathogen.

E. coli strains have traditionally been classified as either commensalor pathogenic, and pathogenic strains are then sub-classified asintestinal or extraintestinal strains. Classification may also be basedon the ‘K’ antigens. The best-studied ‘K’ antigen is ‘K1’, which isconsidered to be the major determinant of virulence among those strainsof E. coli that cause neonatal meningitis. The K1 antigen is ahomopolymer of α-2,8-linked sialic acid, like the capsular saccharide ofserogroup B meningococcus.

More recent taxonomic techniques such as multilocus enzymeelectrophoresis (MLEE) classify E. coli into five phylogenetic groups(A, B1, B2, D & E), and these groupings do not match the traditionalones. For instance, MLEE group B1 includes both commensal and pathogenicstrains, and group D includes both intestinal and extraintestinalstrains.

The extraintestinal pathogenic strains (or ‘ExPEC’ strains [1]) of E.coli fall into MLEE groups B2 and D, and include both uropathogenic(UPEC) strains and meningitis/sepsis-associated (MNEC) strains. UPECstrains cause urinary tract infections (UTIs), and are a common cause ofcystitis. They also cause pyelonephritis (and its complications such assepsis) and catheter-associated infections. MNEC strains cause neonatalmeningitis (0.1 cases per 1000 live births) with case fatality ratesranging from 25 to 40%, and are also responsible for around 1/6 ofsepsis cases.

Most previous ExPEC vaccines have been based on cell lysates or oncellular structures. SOLCOUROVAC™ includes ten different heat-killedbacteria including six ExPEC strains, and a successful phase II clinicaltrial was reported in reference 2. URO-VAXOM™ is an oral tablet vaccinecontaining lyophilised bacterial lysates of 18 selected E. coli strains[3]. Baxter Vaccines developed a UTI vaccine based on pili from 6 to 10different strains, but this product has been abandoned. MedImmunedeveloped a product called MEDI 516 based on the FimH adhesin complex[4], but phase II clinical trials shows inadequate efficacy. Moreover,there was a risk with this vaccine that it would also affectnon-pathogenic FimH^(+ve) strains in the normal intestinal flora, and itwas expected that it would be effective against UPEC strains only,because of its bladder-specific adherence mechanism, leaving other ExPECstrains uncontrolled.

There is thus a need for improved ExPEC vaccines, including a need tomove away from crude cell lysates and towards better-defined molecules,and a need to identify further antigens that are suitable for inclusionin vaccines, particularly antigens that are prevalent among clinicalExpEC strains without also being found in commensal strains. Within theExPEC group, there is a particular need to identify antigens suitablefor immunising against MNEC strains.

One way of addressing these needs was reported in reference 5, where theinventors looked for genes present in genomes of MLEE types B2 and D butabsent from MLEE types A and B1. Further comparative approaches, basedon subtractive hybridisation, have been reported in references 6 & 7.Virulence genes in ExPEC strains have also been identified in reference8. Reference 9 discloses an analysis of four pathogenicity islands inUPEC E. coli strain 536.

Reference 10 used the genome sequence of UPEC (06:K2:H1) strain CFT073[11,12] to identify sequences not present in non-pathogenic E. colistrains. Reference 13 discloses a comparison of the genome sequence ofE. coli human pyelonephritis isolate 536 (06:K15:H31), an UPEC, withsequence data for strains CFT073 (UPEC), EDL933 (enterohemorrhagic) andMG1655 (non-pathogenic laboratory strain). Genome sequences ofpathogenic strains are available in the databases under accessionnumbers AE005174, BA000007 and NC-004431. A sequence from anon-pathogenic strain is available under accession number U00096.

It is an object of the invention to provide further antigens for use inimmunisation against pathogenic E. coli strains, particularly againstExPEC strains, and more particularly against MNEC strains.

SUMMARY OF THE INVENTION

The inventors have identified various open reading frames from a strainof E. coli responsible for neonatal meningitis (MNEC), and haveidentified a subset of these that is of particular interest forpreparing compositions for immunising against MNEC infections.

In one aspect, the present invention relates to a polypeptidecomprising: (a) an amino acid sequence selected from the groupconsisting of SEQ ID NOs 706, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22,24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58,60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94,96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124,126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152,154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180,182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208,210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236,238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264,266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292,294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320,322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348,350, 352, 354, 356, 358, 360, 362, 364, 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8554,8556, 8558, 8560, 8562, 8564, 8566, 8568, 8570, 8572, 8574, 8576, 8578,8580, 8582, 8584, 8586, 8588, 8590, 8592, 8594, 8596, 8598, 8600, 8602,8604, 8606, 8608, 8610, 8612, 8614, 8616, 8618, 8620, 8622, 8624, 8626,8628, 8630, 8632, 8634, 8636, 8638, 8640, 8642, 8644, 8646, 8648, 8650,8652, 8654, 8656, 8658, 8660, 8662, 8664, 8666, 8668, 8670, 8672, 8674,8676, 8678, 8680, 8682, 8684, 8686, 8688, 8690, 8692, 8694, 8696, 8698,8700, 8702, 8704, 8706, 8708, 8710, 8712, 8714, 8716, 8718, 8720, 8722,8724, 8726, 8728, 8730, 8732, 8734, 8736, 8738, 8740, 8742, 8744, 8746,8748, 8750, 8752, 8754, 8756, 8758, 8760, 8762, 8764, 8766, 8768, 8770,8772, 8774, 8776, 8778, 8780, 8782, 8784, 8786, 8788, 8790, 8792, 8794,8796, 8798, 8800, 8802, 8804, 8806, 8808, 8810, 8812, 8814, 8816, 8818,8820, 8822, 8824, 8826, 8828, 8830, 8832, 8834, 8836, 8838, 8840, 8842,8844, 8846, 8848, 8850, 8852, 8854, 8856, 8858, 8860, 8862, 8864, 8866,8868, 8870, 8872, 8874, 8876, 8878, 8880, 8882, 8884, 8886, 8888, 8890,8892, 8894, 8896, 8898, 8900, 8902, 8904, 8906, 8908, 8910, 8912, 8914,8916, 8918, 8920, 8922, 8924, 8926, 8928, 8930, 8932, 8934, 8936, 8938,8940, 8942, 8944, 8946, 8948, 8950, 8952, 8954, 8956, 8958, 8960, 8962,8964, 8966, 8968, 8970, 8972, 8974, 8976, 8978, 8980, 8982, 8984, 8986,8988, 8990, 8992, 8994, 8996, 8998, 9000, 9002, 9004, 9006, 9008, 9010,9012, 9014, 9016, 9018, 9020, 9022, 9024, 9026, 9028, 9030, 9032, 9034,9036, 9038, 9040, 9042, 9044, 9046, 9048, 9050, 9052, 9054, 9056, 9058,9060, 9062, 9064, 9066, 9068, 9070, 9072, 9074, 9076, 9078, 9080, 9082,9084, 9086, 9088, 9090, 9092, 9094, 9096, 9098, 9100, 9102, 9104, 9106,9108, 9110, 9112, 9114, 9116, 9118, 9120, 9122, 9124, 9126, 9128, 9130,9132, 9134, 9136, 9138, 9140, 9142, 9144, 9146, 9148, 9150, 9152, 9154,9156, 9158, 9160, 9162, 9164, 9166, 9168, 9170, 9172, 9174, 9176, 9178,9180, 9182, 9184, 9186, 9188, 9190, 9192, 9194, 9196, 9198, 9200, 9202,9204, 9206, 9208, 9210, 9212, 9214, 9216, 9218, 9220, 9222, 9224, 9226,9228, 9230, 9232, 9234, 9236, 9238, 9240, 9242, 9244, 9246, 9248, 9250,9252, 9254, 9256, 9258, 9260, 9262, 9264, 9266, 9268, 9270, 9272, 9274,9276, 9278, 9280, 9282, 9284, 9286, 9288, 9290, 9292, 9294, 9296, 9298,9300, 9302, 9304, 9306, 9308, 9310, 9312, 9314, 9316, 9318, 9320, 9322,9324, 9326, 9328, 9330, 9332, 9334, 9336, 9338, 9340, 9342, 9344, 9346,9348, 9350, 9352, 9354, 9356, 9358, 9360, 9362, 9364, 9366, 9368, 9370,9372, 9374, 9376, 9378, 9380, 9382, 9384, 9386, 9388, 9390, 9392, 9394,9396, 9398, 9400, 9402, 9404, 9406, 9408, 9410, 9412, 9414, 9416, 9418,9420, 9422, 9424, 9426, 9428, 9430, 9432, 9434, 9436, 9438, 9440, 9442,9444, 9446, 9448, 9450, 9452, 9454, 9456, 9458, 9460, 9462, 9464, 9466,9468, 9470, 9472, 9474, 9476, 9478, 9480, 9482, 9484, 9486, 9488, 9490,9492, 9494, 9496, 9498, 9500, 9502, 9504, 9506, 9508, 9510, 9512, 9514,9516, 9518, 9520, 9522, 9524, 9526, 9528, 9530, 9532, 9534, 9536, 9538,9540, 9542, 9544, 9546, 9548, 9550, 9552, 9554, 9556, 9558, 9560, 9562,9564, 9566, 9568, 9570, 9572, 9574, 9576, 9578, 9580, 9582, 9584, 9586,9588, 9590, 9592, 9594, 9596, 9598, 9600, 9602, 9604, 9606, 9608, 9610,9612, 9614, 9616, 9618, 9620, 9622, 9624, 9626, 9628, 9630, 9632, 9634,9636, 9638, 9640, 9642, 9644, 9646, 9648, 9650, 9652, 9654, 9656, 9658,9660, 9662, 9664, 9666, 9668, 9670, 9672, 9674, 9676, 9678, 9680, 9682,9684, 9686, 9688, 9690, 9692, 9694, 9696, 9698, 9700, 9702, 9704, 9706,9708, 9710, 9712, 9714, 9716, 9718, 9720, 9722, 9724, 9726, 9728, 9730,9732, 9734, 9736, 9738, 9740, 9742, 9744, 9746, 9748, 9750, 9752, 9754,9756, 9758, 9760, 9762, 9764, 9766, 9768, 9770, 9772, 9774, 9776, 9778,9780, 9782, 9784, 9786, 9788, 9790, 9792, 9794, 9796, 9798, 9800, 9802,9804, 9806, 9808, 9810, 9812, 9814, 9816, 9818, 9820, 9822, 9824, 9826,9828, 9830, 9832, 9834, 9836, 9838, 9840, 9842, 9844, 9846, 9848, 9850,9852, 9854, 9856, 9858, 9860, 9862, 9864, 9866, 9868, 9870, 9872, 9874,9876, 9878, 9880, 9882, 9884, 9886, 9888, 9890, 9892, 9894, 9896, 9898,9900, 9902, 9904, 9906, 9908, 9910, 9912, 9914, 9916, 9918, 9920, 9922,9924, 9926, 9928, 9930, 9932, 9934, 9936, 9938, 9940, 9942, 9944, 9946,9948, 9950, 9952, 9954, 9956, 9958, 9960, 9962, 9964, 9966, 9968, 9970,9972, 9974, 9976, 9978, 9980, 9982, 9984, 9986, 9988, 9990; (b) an aminoacid sequence having at least 80% sequence identity to an amino acidsequence of (a); (c) an amino acid sequence which is a fragment of atleast 10 consecutive amino acids from an amino acid sequence of (a); or(d) an amino acid sequence having at least 80% sequence identity to anamino acid sequence of (a) and including a fragment of at least 10consecutive amino acids from an amino acid sequence of (a). In aparticular embodiment, the polypeptides of this aspect of the inventioncomprise a fragment which comprises at least one B-cell epitope of (a).In a second aspect, the invention relates to a polypeptide comprising:(a) an amino acid sequence selected from the group consisting of SEQ IDNOs 512, 2454, 2456, 2678, 2692, 4596, 4748, 7004, 7052, 8168, 4628 and5700; (b) an amino acid sequence having at least 80% sequence identityto an amino acid sequence of (a); (c) an amino acid sequence which is afragment of at least 10 consecutive amino acids from an amino acidsequence of (a); or (d) an amino acid sequence having at least 80%sequence identity to an amino acid sequence of (a) and including afragment of at least 10 consecutive amino acids from an amino acidsequence of (a). In a particular embodiment, the polypeptides of thisaspect of the invention comprise a fragment which comprises at least oneB-cell epitope of (a).

In another aspect, the invention relates to a polypeptide comprising:(a) an amino acid sequence selected from the group consisting of SEQ IDNOs 8564, 2728, 9872, 4672, 5680, 534, 3222, 3226 and 7004; (b) an aminoacid sequence having at least 80% sequence identity to an amino acidsequence of (a); (c) an amino acid sequence which is a fragment of atleast 10 consecutive amino acids from an amino acid sequence of (a); or(d) an amino acid sequence having at least 80% sequence identity to anamino acid sequence of (a) and including a fragment of at least 10consecutive amino acids from an amino acid sequence of (a). In aparticular embodiment, the polypeptides of this aspect of the inventioncomprise a fragment which comprises at least one B-cell epitope of (a).

The present invention also relates to a nucleic acid comprising: (a) anucleotide sequence selected from the group consisting of SEQ ID NOs705, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35,37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71,73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105,107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133,135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161,163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189,191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217,219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245,247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273,275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301,303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329,331, 333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357,359, 361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385,387, 389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413,415, 417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441,443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469,471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497,499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525,527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553,555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581,583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609,611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637,639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665,667, 669, 671, 673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693,695, 697, 699, 701, 703, 707, 709, 711, 713, 715, 717, 719, 721, 723,725, 727, 729, 731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751,753, 755, 757, 759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779,781, 783, 785, 787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807,809, 811, 813, 815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835,837, 839, 841, 843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863,865, 867, 869, 871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891,893, 895, 897, 899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919,921, 923, 925, 927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947,949, 951, 953, 955, 957, 959, 961, 963, 965, 967, 969, 971, 973, 975,977, 979, 981, 983, 985, 987, 989, 991, 993, 995, 997, 999, 1001, 1003,1005, 1007, 1009, 1011, 1013, 1015, 1017, 1019, 1021, 1023, 1025, 1027,1029, 1031, 1033, 1035, 1037, 1039, 1041, 1043, 1045, 1047, 1049, 1051,1053, 1055, 1057, 1059, 1061, 1063, 1065, 1067, 1069, 1071, 1073, 1075,1077, 1079, 1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099,1101, 1103, 1105, 1107, 1109, 1111, 1113, 1115, 1117, 1119, 1121, 1123,1125, 1127, 1129, 1131, 1133, 1135, 1137, 1139, 1141, 1143, 1145, 1147,1149, 1151, 1153, 1155, 1157, 1159, 1161, 1163, 1165, 1167, 1169, 1171,1173, 1175, 1177, 1179, 1181, 1183, 1185, 1187, 1189, 1191, 1193, 1195,1197, 1199, 1201, 1203, 1205, 1207, 1209, 1211, 1213, 1215, 1217, 1219,1221, 1223, 1225, 1227, 1229, 1231, 1233, 1235, 1237, 1239, 1241, 1243,1245, 1247, 1249, 1251, 1253, 1255, 1257, 1259, 1261, 1263, 1265, 1267,1269, 1271, 1273, 1275, 1277, 1279, 1281, 1283, 1285, 1287, 1289, 1291,1293, 1295, 1297, 1299, 1301, 1303, 1305, 1307, 1309, 1311, 1313, 1315,1317, 1319, 1321, 1323, 1325, 1327, 1329, 1331, 1333, 1335, 1337, 1339,1341, 1343, 1345, 1347, 1349, 1351, 1353, 1355, 1357, 1359, 1361, 1363,1365, 1367, 1369, 1371, 1373, 1375, 1377, 1379, 1381, 1383, 1385, 1387,1389, 1391, 1393, 1395, 1397, 1399, 1401, 1403, 1405, 1407, 1409, 1411,1413, 1415, 1417, 1419, 1421, 1423, 1425, 1427, 1429, 1431, 1433, 1435,1437, 1439, 1441, 1443, 1445, 1447, 1449, 1451, 1453, 1455, 1457, 1459,1461, 1463, 1465, 1467, 1469, 1471, 1473, 1475, 1477, 1479, 1481, 1483,1485, 1487, 1489, 1491, 1493, 1495, 1497, 1499, 1501, 1503, 1505, 1507,1509, 1511, 1513, 1515, 1517, 1519, 1521, 1523, 1525, 1527, 1529, 1531,1533, 1535, 1537, 1539, 1541, 1543, 1545, 1547, 1549, 1551, 1553, 1555,1557, 1559, 1561, 1563, 1565, 1567, 1569, 1571, 1573, 1575, 1577, 1579,1581, 1583, 1585, 1587, 1589, 1591, 1593, 1595, 1597, 1599, 1601, 1603,1605, 1607, 1609, 1611, 1613, 1615, 1617, 1619, 1621, 1623, 1625, 1627,1629, 1631, 1633, 1635, 1637, 1639, 1641, 1643, 1645, 1647, 1649, 1651,1653, 1655, 1657, 1659, 1661, 1663, 1665, 1667, 1669, 1671, 1673, 1675,1677, 1679, 1681, 1683, 1685, 1687, 1689, 1691, 1693, 1695, 1697, 1699,1701, 1703, 1705, 1707, 1709, 1711, 1713, 1715, 1717, 1719, 1721, 1723,1725, 1727, 1729, 1731, 1733, 1735, 1737, 1739, 1741, 1743, 1745, 1747,1749, 1751, 1753, 1755, 1757, 1759, 1761, 1763, 1765, 1767, 1769, 1771,1773, 1775, 1777, 1779, 1781, 1783, 1785, 1787, 1789, 1791, 1793, 1795,1797, 1799, 1801, 1803, 1805, 1807, 1809, 1811, 1813, 1815, 1817, 1819,1821, 1823, 1825, 1827, 1829, 1831, 1833, 1835, 1837, 1839, 1841, 1843,1845, 1847, 1849, 1851, 1853, 1855, 1857, 1859, 1861, 1863, 1865, 1867,1869, 1871, 1873, 1875, 1877, 1879, 1881, 1883, 1885, 1887, 1889, 1891,1893, 1895, 1897, 1899, 1901, 1903, 1905, 1907, 1909, 1911, 1913, 1915,1917, 1919, 1921, 1923, 1925, 1927, 1929, 1931, 1933, 1935, 1937, 1939,1941, 1943, 1945, 1947, 1949, 1951, 1953, 1955, 1957, 1959, 1961, 1963,1965, 1967, 1969, 1971, 1973, 1975, 1977, 1979, 1981, 1983, 1985, 1987,1989, 1991, 1993, 1995, 1997, 1999, 2001, 2003, 2005, 2007, 2009, 2011,2013, 2015, 2017, 2019, 2021, 2023, 2025, 2027, 2029, 2031, 2033, 2035,2037, 2039, 2041, 2043, 2045, 2047, 2049, 2051, 2053, 2055, 2057, 2059,2061, 2063, 2065, 2067, 2069, 2071, 2073, 2075, 2077, 2079, 2081, 2083,2085, 2087, 2089, 2091, 2093, 2095, 2097, 2099, 2101, 2103, 2105, 2107,2109, 2111, 2113, 2115, 2117, 2119, 2121, 2123, 2125, 2127, 2129, 2131,2133, 2135, 2137, 2139, 2141, 2143, 2145, 2147, 2149, 2151, 2153, 2155,2157, 2159, 2161, 2163, 2165, 2167, 2169, 2171, 2173, 2175, 2177, 2179,2181, 2183, 2185, 2187, 2189, 2191, 2193, 2195, 2197, 2199, 2201, 2203,2205, 2207, 2209, 2211, 2213, 2215, 2217, 2219, 2221, 2223, 2225, 2227,2229, 2231, 2233, 2235, 2237, 2239, 2241, 2243, 2245, 2247, 2249, 2251,2253, 2255, 2257, 2259, 2261, 2263, 2265, 2267, 2269, 2271, 2273, 2275,2277, 2279, 2281, 2283, 2285, 2287, 2289, 2291, 2293, 2295, 2297, 2299,2301, 2303, 2305, 2307, 2309, 2311, 2313, 2315, 2317, 2319, 2321, 2323,2325, 2327, 2329, 2331, 2333, 2335, 2337, 2339, 2341, 2343, 2345, 2347,2349, 2351, 2353, 2355, 2357, 2359, 2361, 2363, 2365, 2367, 2369, 2371,2373, 2375, 2377, 2379, 2381, 2383, 2385, 2387, 2389, 2391, 2393, 2395,2397, 2399, 2401, 2403, 2405, 2407, 2409, 2411, 2413, 2415, 2417, 2419,2421, 2423, 2425, 2427, 2429, 2431, 2433, 2435, 2437, 2439, 2441, 2443,2445, 2447, 2449, 2451, 2453, 2455, 2457, 2459, 2461, 2463, 2465, 2467,2469, 2471, 2473, 2475, 2477, 2479, 2481, 2483, 2485, 2487, 2489, 2491,2493, 2495, 2497, 2499, 2501, 2503, 2505, 2507, 2509, 2511, 2513, 2515,2517, 2519, 2521, 2523, 2525, 2527, 2529, 2531, 2533, 2535, 2537, 2539,2541, 2543, 2545, 2547, 2549, 2551, 2553, 2555, 2557, 2559, 2561, 2563,2565, 2567, 2569, 2571, 2573, 2575, 2577, 2579, 2581, 2583, 2585, 2587,2589, 2591, 2593, 2595, 2597, 2599, 2601, 2603, 2605, 2607, 2609, 2611,2613, 2615, 2617, 2619, 2621, 2623, 2625, 2627, 2629, 2631, 2633, 2635,2637, 2639, 2641, 2643, 2645, 2647, 2649, 2651, 2653, 2655, 2657, 2659,2661, 2663, 2665, 2667, 2669, 2671, 2673, 2675, 2677, 2679, 2681, 2683,2685, 2687, 2689, 2691, 2693, 2695, 2697, 2699, 2701, 2703, 2705, 2707,2709, 2711, 2713, 2715, 2717, 2719, 2721, 2723, 2725, 2727, 2729, 2731,2733, 2735, 2737, 2739, 2741, 2743, 2745, 2747, 2749, 2751, 2753, 2755,2757, 2759, 2761, 2763, 2765, 2767, 2769, 2771, 2773, 2775, 2777, 2779,2781, 2783, 2785, 2787, 2789, 2791, 2793, 2795, 2797, 2799, 2801, 2803,2805, 2807, 2809, 2811, 2813, 2815, 2817, 2819, 2821, 2823, 2825, 2827,2829, 2831, 2833, 2835, 2837, 2839, 2841, 2843, 2845, 2847, 2849, 2851,2853, 2855, 2857, 2859, 2861, 2863, 2865, 2867, 2869, 2871, 2873, 2875,2877, 2879, 2881, 2883, 2885, 2887, 2889, 2891, 2893, 2895, 2897, 2899,2901, 2903, 2905, 2907, 2909, 2911, 2913, 2915, 2917, 2919, 2921, 2923,2925, 2927, 2929, 2931, 2933, 2935, 2937, 2939, 2941, 2943, 2945, 2947,2949, 2951, 2953, 2955, 2957, 2959, 2961, 2963, 2965, 2967, 2969, 2971,2973, 2975, 2977, 2979, 2981, 2983, 2985, 2987, 2989, 2991, 2993, 2995,2997, 2999, 3001, 3003, 3005, 3007, 3009, 3011, 3013, 3015, 3017, 3019,3021, 3023, 3025, 3027, 3029, 3031, 3033, 3035, 3037, 3039, 3041, 3043,3045, 3047, 3049, 3051, 3053, 3055, 3057, 3059, 3061, 3063, 3065, 3067,3069, 3071, 3073, 3075, 3077, 3079, 3081, 3083, 3085, 3087, 3089, 3091,3093, 3095, 3097, 3099, 3101, 3103, 3105, 3107, 3109, 3111, 3113, 3115,3117, 3119, 3121, 3123, 3125, 3127, 3129, 3131, 3133, 3135, 3137, 3139,3141, 3143, 3145, 3147, 3149, 3151, 3153, 3155, 3157, 3159, 3161, 3163,3165, 3167, 3169, 3171, 3173, 3175, 3177, 3179, 3181, 3183, 3185, 3187,3189, 3191, 3193, 3195, 3197, 3199, 3201, 3203, 3205, 3207, 3209, 3211,3213, 3215, 3217, 3219, 3221, 3223, 3225, 3227, 3229, 3231, 3233, 3235,3237, 3239, 3241, 3243, 3245, 3247, 3249, 3251, 3253, 3255, 3257, 3259,3261, 3263, 3265, 3267, 3269, 3271, 3273, 3275, 3277, 3279, 3281, 3283,3285, 3287, 3289, 3291, 3293, 3295, 3297, 3299, 3301, 3303, 3305, 3307,3309, 3311, 3313, 3315, 3317, 3319, 3321, 3323, 3325, 3327, 3329, 3331,3333, 3335, 3337, 3339, 3341, 3343, 3345, 3347, 3349, 3351, 3353, 3355,3357, 3359, 3361, 3363, 3365, 3367, 3369, 3371, 3373, 3375, 3377, 3379,3381, 3383, 3385, 3387, 3389, 3391, 3393, 3395, 3397, 3399, 3401, 3403,3405, 3407, 3409, 3411, 3413, 3415, 3417, 3419, 3421, 3423, 3425, 3427,3429, 3431, 3433, 3435, 3437, 3439, 3441, 3443, 3445, 3447, 3449, 3451,3453, 3455, 3457, 3459, 3461, 3463, 3465, 3467, 3469, 3471, 3473, 3475,3477, 3479, 3481, 3483, 3485, 3487, 3489, 3491, 3493, 3495, 3497, 3499,3501, 3503, 3505, 3507, 3509, 3511, 3513, 3515, 3517, 3519, 3521, 3523,3525, 3527, 3529, 3531, 3533, 3535, 3537, 3539, 3541, 3543, 3545, 3547,3549, 3551, 3553, 3555, 3557, 3559, 3561, 3563, 3565, 3567, 3569, 3571,3573, 3575, 3577, 3579, 3581, 3583, 3585, 3587, 3589, 3591, 3593, 3595,3597, 3599, 3601, 3603, 3605, 3607, 3609, 3611, 3613, 3615, 3617, 3619,3621, 3623, 3625, 3627, 3629, 3631, 3633, 3635, 3637, 3639, 3641, 3643,3645, 3647, 3649, 3651, 3653, 3655, 3657, 3659, 3661, 3663, 3665, 3667,3669, 3671, 3673, 3675, 3677, 3679, 3681, 3683, 3685, 3687, 3689, 3691,3693, 3695, 3697, 3699, 3701, 3703, 3705, 3707, 3709, 3711, 3713, 3715,3717, 3719, 3721, 3723, 3725, 3727, 3729, 3731, 3733, 3735, 3737, 3739,3741, 3743, 3745, 3747, 3749, 3751, 3753, 3755, 3757, 3759, 3761, 3763,3765, 3767, 3769, 3771, 3773, 3775, 3777, 3779, 3781, 3783, 3785, 3787,3789, 3791, 3793, 3795, 3797, 3799, 3801, 3803, 3805, 3807, 3809, 3811,3813, 3815, 3817, 3819, 3821, 3823, 3825, 3827, 3829, 3831, 3833, 3835,3837, 3839, 3841, 3843, 3845, 3847, 3849, 3851, 3853, 3855, 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7891,7893, 7895, 7897, 7899, 7901, 7903, 7905, 7907, 7909, 7911, 7913, 7915,7917, 7919, 7921, 7923, 7925, 7927, 7929, 7931, 7933, 7935, 7937, 7939,7941, 7943, 7945, 7947, 7949, 7951, 7953, 7955, 7957, 7959, 7961, 7963,7965, 7967, 7969, 7971, 7973, 7975, 7977, 7979, 7981, 7983, 7985, 7987,7989, 7991, 7993, 7995, 7997, 7999, 8001, 8003, 8005, 8007, 8009, 8011,8013, 8015, 8017, 8019, 8021, 8023, 8025, 8027, 8029, 8031, 8033, 8035,8037, 8039, 8041, 8043, 8045, 8047, 8049, 8051, 8053, 8055, 8057, 8059,8061, 8063, 8065, 8067, 8069, 8071, 8073, 8075, 8077, 8079, 8081, 8083,8085, 8087, 8089, 8091, 8093, 8095, 8097, 8099, 8101, 8103, 8105, 8107,8109, 8111, 8113, 8115, 8117, 8119, 8121, 8123, 8125, 8127, 8129, 8131,8133, 8135, 8137, 8139, 8141, 8143, 8145, 8147, 8149, 8151, 8153, 8155,8157, 8159, 8161, 8163, 8165, 8167, 8169, 8171, 8173, 8175, 8177, 8179,8181, 8183, 8185, 8187, 8189, 8191, 8193, 8195, 8197, 8199, 8201, 8203,8205, 8207, 8209, 8211, 8213, 8215, 8217, 8219, 8221, 8223, 8225, 8227,8229, 8231, 8233, 8235, 8237, 8239, 8241, 8243, 8245, 8247, 8249, 8251,8253, 8255, 8257, 8259, 8261, 8263, 8265, 8267, 8269, 8271, 8273, 8275,8277, 8279, 8281, 8283, 8285, 8287, 8289, 8291, 8293, 8295, 8297, 8299,8301, 8303, 8305, 8307, 8309, 8311, 8313, 8315, 8317, 8319, 8321, 8323,8325, 8327, 8329, 8331, 8333, 8335, 8337, 8339, 8341, 8343, 8345, 8347,8349, 8351, 8353, 8355, 8357, 8359, 8361, 8363, 8365, 8367, 8369, 8371,8373, 8375, 8377, 8379, 8381, 8383, 8385, 8387, 8389, 8391, 8393, 8395,8397, 8399, 8401, 8403, 8405, 8407, 8409, 8411, 8413, 8415, 8417, 8419,8421, 8423, 8425, 8427, 8429, 8431, 8433, 8435, 8437, 8439, 8441, 8443,8445, 8447, 8449, 8451, 8453, 8455, 8457, 8459, 8461, 8463, 8465, 8467,8469, 8471, 8473, 8475, 8477, 8479, 8481, 8483, 8485, 8487, 8489, 8491,8493, 8495, 8497, 8499, 8501, 8503, 8505, 8507, 8509, 8511, 8513, 8515,8517, 8519, 8521, 8523, 8525, 8527, 8529, 8531, 8533, 8535, 8537, 8539,8541, 8543, 8545, 8547, 8549, 8551, 8553, 8555, 8557, 8559, 8561, 8563,8565, 8567, 8569, 8571, 8573, 8575, 8577, 8579, 8581, 8583, 8585, 8587,8589, 8591, 8593, 8595, 8597, 8599, 8601, 8603, 8605, 8607, 8609, 8611,8613, 8615, 8617, 8619, 8621, 8623, 8625, 8627, 8629, 8631, 8633, 8635,8637, 8639, 8641, 8643, 8645, 8647, 8649, 8651, 8653, 8655, 8657, 8659,8661, 8663, 8665, 8667, 8669, 8671, 8673, 8675, 8677, 8679, 8681, 8683,8685, 8687, 8689, 8691, 8693, 8695, 8697, 8699, 8701, 8703, 8705, 8707,8709, 8711, 8713, 8715, 8717, 8719, 8721, 8723, 8725, 8727, 8729, 8731,8733, 8735, 8737, 8739, 8741, 8743, 8745, 8747, 8749, 8751, 8753, 8755,8757, 8759, 8761, 8763, 8765, 8767, 8769, 8771, 8773, 8775, 8777, 8779,8781, 8783, 8785, 8787, 8789, 8791, 8793, 8795, 8797, 8799, 8801, 8803,8805, 8807, 8809, 8811, 8813, 8815, 8817, 8819, 8821, 8823, 8825, 8827,8829, 8831, 8833, 8835, 8837, 8839, 8841, 8843, 8845, 8847, 8849, 8851,8853, 8855, 8857, 8859, 8861, 8863, 8865, 8867, 8869, 8871, 8873, 8875,8877, 8879, 8881, 8883, 8885, 8887, 8889, 8891, 8893, 8895, 8897, 8899,8901, 8903, 8905, 8907, 8909, 8911, 8913, 8915, 8917, 8919, 8921, 8923,8925, 8927, 8929, 8931, 8933, 8935, 8937, 8939, 8941, 8943, 8945, 8947,8949, 8951, 8953, 8955, 8957, 8959, 8961, 8963, 8965, 8967, 8969, 8971,8973, 8975, 8977, 8979, 8981, 8983, 8985, 8987, 8989, 8991, 8993, 8995,8997, 8999, 9001, 9003, 9005, 9007, 9009, 9011, 9013, 9015, 9017, 9019,9021, 9023, 9025, 9027, 9029, 9031, 9033, 9035, 9037, 9039, 9041, 9043,9045, 9047, 9049, 9051, 9053, 9055, 9057, 9059, 9061, 9063, 9065, 9067,9069, 9071, 9073, 9075, 9077, 9079, 9081, 9083, 9085, 9087, 9089, 9091,9093, 9095, 9097, 9099, 9101, 9103, 9105, 9107, 9109, 9111, 9113, 9115,9117, 9119, 9121, 9123, 9125, 9127, 9129, 9131, 9133, 9135, 9137, 9139,9141, 9143, 9145, 9147, 9149, 9151, 9153, 9155, 9157, 9159, 9161, 9163,9165, 9167, 9169, 9171, 9173, 9175, 9177, 9179, 9181, 9183, 9185, 9187,9189, 9191, 9193, 9195, 9197, 9199, 9201, 9203, 9205, 9207, 9209, 9211,9213, 9215, 9217, 9219, 9221, 9223, 9225, 9227, 9229, 9231, 9233, 9235,9237, 9239, 9241, 9243, 9245, 9247, 9249, 9251, 9253, 9255, 9257, 9259,9261, 9263, 9265, 9267, 9269, 9271, 9273, 9275, 9277, 9279, 9281, 9283,9285, 9287, 9289, 9291, 9293, 9295, 9297, 9299, 9301, 9303, 9305, 9307,9309, 9311, 9313, 9315, 9317, 9319, 9321, 9323, 9325, 9327, 9329, 9331,9333, 9335, 9337, 9339, 9341, 9343, 9345, 9347, 9349, 9351, 9353, 9355,9357, 9359, 9361, 9363, 9365, 9367, 9369, 9371, 9373, 9375, 9377, 9379,9381, 9383, 9385, 9387, 9389, 9391, 9393, 9395, 9397, 9399, 9401, 9403,9405, 9407, 9409, 9411, 9413, 9415, 9417, 9419, 9421, 9423, 9425, 9427,9429, 9431, 9433, 9435, 9437, 9439, 9441, 9443, 9445, 9447, 9449, 9451,9453, 9455, 9457, 9459, 9461, 9463, 9465, 9467, 9469, 9471, 9473, 9475,9477, 9479, 9481, 9483, 9485, 9487, 9489, 9491, 9493, 9495, 9497, 9499,9501, 9503, 9505, 9507, 9509, 9511, 9513, 9515, 9517, 9519, 9521, 9523,9525, 9527, 9529, 9531, 9533, 9535, 9537, 9539, 9541, 9543, 9545, 9547,9549, 9551, 9553, 9555, 9557, 9559, 9561, 9563, 9565, 9567, 9569, 9571,9573, 9575, 9577, 9579, 9581, 9583, 9585, 9587, 9589, 9591, 9593, 9595,9597, 9599, 9601, 9603, 9605, 9607, 9609, 9611, 9613, 9615, 9617, 9619,9621, 9623, 9625, 9627, 9629, 9631, 9633, 9635, 9637, 9639, 9641, 9643,9645, 9647, 9649, 9651, 9653, 9655, 9657, 9659, 9661, 9663, 9665, 9667,9669, 9671, 9673, 9675, 9677, 9679, 9681, 9683, 9685, 9687, 9689, 9691,9693, 9695, 9697, 9699, 9701, 9703, 9705, 9707, 9709, 9711, 9713, 9715,9717, 9719, 9721, 9723, 9725, 9727, 9729, 9731, 9733, 9735, 9737, 9739,9741, 9743, 9745, 9747, 9749, 9751, 9753, 9755, 9757, 9759, 9761, 9763,9765, 9767, 9769, 9771, 9773, 9775, 9777, 9779, 9781, 9783, 9785, 9787,9789, 9791, 9793, 9795, 9797, 9799, 9801, 9803, 9805, 9807, 9809, 9811,9813, 9815, 9817, 9819, 9821, 9823, 9825, 9827, 9829, 9831, 9833, 9835,9837, 9839, 9841, 9843, 9845, 9847, 9849, 9851, 9853, 9855, 9857, 9859,9861, 9863, 9865, 9867, 9869, 9871, 9873, 9875, 9877, 9879, 9881, 9883,9885, 9887, 9889, 9891, 9893, 9895, 9897, 9899, 9901, 9903, 9905, 9907,9909, 9911, 9913, 9915, 9917, 9919, 9921, 9923, 9925, 9927, 9929, 9931,9933, 9935, 9937, 9939, 9941, 9943, 9945, 9947, 9949, 9951, 9953, 9955,9957, 9959, 9961, 9963, 9965, 9967, 9969, 9971, 9973, 9975, 9977, 9979,9981, 9983, 9985, 9987, 9989; (b) a nucleotide sequence having at least80% sequence identity to an amino acid sequence of (a); (c) a nucleotidesequence which is a fragment of at least 10 consecutive nucleotides froma nucleotide sequence of (a); or (d) a nucleotide sequence having atleast 80% sequence identity to a nucleotide sequence of (a) andincluding a fragment of at least 10 consecutive nucleotides from anucleotide sequence of (a). In another aspect, the invention relates toa nucleic acid comprising: (a) a nucleotide sequence selected from thegroup consisting of SEQ ID NOs 511, 2453, 2455, 2677, 2691, 4595, 4747,7003, 7051, 8167, 4627 and 5699; (b) a nucleotide sequence having atleast 80% sequence identity to an amino acid sequence of (a); (c) anucleotide sequence which is a fragment of at least 10 consecutivenucleotides from a nucleotide sequence of (a); or (d) a nucleotidesequence having at least 80% sequence identity to a nucleotide sequenceof (a) and including a fragment of at least 10 consecutive nucleotidesfrom a nucleotide sequence of (a).

In a further aspect, the invention relates to a nucleic acid comprising:(a) a nucleotide sequence selected from the group consisting of SEQ IDNos 8563, 2727, 9871, 4671, 5679, 533, 3221, 3225 and 7003; (b) anucleotide sequence having at least 80% sequence identity to an aminoacid sequence of (a); (c) a nucleotide sequence which is a fragment ofat least 10 consecutive nucleotides from a nucleotide sequence of (a);or (d) a nucleotide sequence having at least 80% sequence identity to anucleotide sequence of (a) and including a fragment of at least 10consecutive nucleotides from a nucleotide sequence of (a).

The present invention also relates to nucleic acids of the inventionencoding a polypeptide the invention, and to monoclonal antibodiesspecific for a polypeptide of the invention.

The polypeptides, nucleic acids and antibodies of the invention can beused in medicine and in the manufacture of a medicament for raising animmune response in a patient.

The present invention also relates to pharmaceutical compositionscomprising a polypeptide, nucleic acid or antibody of the invention, inadmixture with a pharmaceutically acceptable carrier. The inventionfurther relates to a pharmaceutical composition comprising two or morepolypeptides of the invention, in admixture with a pharmaceuticallyacceptable carrier. In a particular embodiment, the pharmaceuticalcompositions of the invention further comprise a vaccine adjuvant.

The present invention further relates to immunogenic compositionscomprising one or more outer membrane vesicles (OMVs) expressing oroverexpressing one or more polypeptides encoded by:

(a) a nucleotide sequence selected from the group consisting of SEQ IDNOs 511, 2453, 2455, 2677, 2691, 4595, 4747, 7003, 7051, 8167, 4627 and5699; (b) a nucleotide sequence having at least 80% sequence identity toan amino acid sequence of (a); (c) a nucleotide sequence which is afragment of at least 10 consecutive nucleotides from a nucleotidesequence of (a); or (d) a nucleotide sequence having at least 80%sequence identity to a nucleotide sequence of (a) and including afragment of at least 10 consecutive nucleotides from a nucleotidesequence of (a).

The present invention also relates to immunogenic compositionscomprising one or more OMVs expressing or overexpressing one or morepolypeptides encoded by: (a) a nucleotide sequence selected from thegroup consisting of SEQ ID Nos 8563, 2727, 9871, 4671, 5679, 533, 3221,3225 and 7003; (b) a nucleotide sequence having at least 80% sequenceidentity to an amino acid sequence of (a); (c) a nucleotide sequencewhich is a fragment of at least 10 consecutive nucleotides from anucleotide sequence of (a); or (d) a nucleotide sequence having at least80% sequence identity to a nucleotide sequence of (a) and including afragment of at least 10 consecutive nucleotides from a nucleotidesequence of (a).

The present invention also relates to methods for raising an immuneresponse in a patient, comprising the step of administering to thepatient a pharmaceutical composition or immunogenic composition of theinvention. In a particular embodiment, the immune response is protectiveagainst ExPEC infection, and in particular against a MNEC infection.

Further aspects of the invention are described below.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the % survival of mice over time after challenge withIHE3034 following immunization with heat-inactivated IHE3034 or with acontrol (‘fisiol’), and also bacteremia levels (cfu/ml) after 24 hours.

FIG. 2 shows the % survival of mice after challenge with IHE3034following immunization either with heat-inactivated bacteria (top) orwith vesicles (bottom) from ΔToIR mutants Immunogens were prepared fromthe IHE3034 strain or from a control strain (DH5).

FIG. 3 shows the % survival of mice after challenge with IHE3034following immunization with IroN or a control (top) and the serumanti-IroN Ig titer (bottom).

FIG. 4 is similar to FIG. 3, but for IbeA rather than IroN.

DETAILED DESCRIPTION OF THE INVENTION

The inventors have identified 4995 open reading frames from a K1/B2 MNECstrain, and have identified a subset of these that is of particularinterest for preparing immunogenic compositions against MNEC strains.

Polypeptides

The invention provides polypeptides comprising the amino acid sequencesdisclosed in the examples. These amino acid sequences are given in thesequence listing as the even-numbered sequences SEQ ID NOs 2 to 9990.

Preferred amino acid sequences from within SEQ ID NOs 2 to 9990 aregiven in Table 2. Further preferred amino acid sequences are those notidentified in the prior art e.g. not identified in any of references 5,6, 7, 8, 9, 10, 11 and 13.

The invention also provides a polypeptide encoded within any one ofnucleic acid sequences SEQ ID NOs 9991 to 10273 or within SEQ ID NO:10274. The coding sequence in the nucleic acid preferably begins with astart codon and ends with a stop codon. Preferred polypeptides are thosenot identified in the prior art e.g. not identified in any of references5, 6, 8, 10 and 11.

The invention also provides polypeptides comprising amino acid sequencesthat have sequence identity to the amino acid sequences disclosed in theexamples. Similarly, the invention provides polypeptides comprisingamino acid sequences that have sequence identity to the amino acidsequences encoded within SEQ ID NOs 9991 to 10273 or within SEQ ID NO:10274. Depending on the particular sequence, the degree of sequenceidentity is preferably greater than 50% (e.g. 60%, 70%, 75%, 80%, 85%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more). Thesepolypeptides include homologs, orthologs, allelic variants and mutants.Typically, 50% identity or more between two polypeptide sequences isconsidered to be an indication of functional equivalence. Identitybetween polypeptides is preferably determined by the Smith-Watermanhomology search algorithm as implemented in the MPSRCH program (OxfordMolecular), using an affine gap search with parameters gap openpenalty=12 and gap extension penalty=1.

These polypeptide may, compared to the sequences of the examples,include one or more (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.)conservative amino acid substitutions i.e. replacements of one aminoacid with another which has a related side chain. Genetically-encodedamino acids are generally divided into four families: (1) acidic i.e.aspartate, glutamate; (2) basic i.e. lysine, arginine, histidine; (3)non-polar i.e. alanine, valine, leucine, isoleucine, proline,phenylalanine, methionine, tryptophan; and (4) uncharged polar i.e.glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine.Phenylalanine, tryptophan, and tyrosine are sometimes classified jointlyas aromatic amino acids. In general, substitution of single amino acidswithin these families does not have a major effect on the biologicalactivity. Moreover, the polypeptides may have one or more (e.g. 1, 2, 3,4, 5, 6, 7, 8, 9, 10, etc.) single amino acid deletions relative to areference sequence. Furthermore, the polypeptides may include one ormore (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.) insertions (e.g. each of1, 2, 3, 4 or 5 amino acids) relative to a reference sequence.

Preferred polypeptides include polypeptides that are lipidated, that arelocated in the outer membrane, that are located in the inner membrane,or that are located in the periplasm. Particularly preferredpolypeptides are those that fall into more than one of these categoriese.g. lipidated polypeptides that are located in the outer membrane.Lipoproteins may have a N-terminal cysteine to which lipid is covalentlyattached, following post-translational processing of the signal peptide.

Polypeptides that may be lipidated include SEQ ID NOs: 620, 630, 660,724, 946, 1196, 1304, 1510, 1530, 1548, 1600, 1700, 1958, 2030, 2396,2610, 3040, 3364, 3414, 3422, 3554, 3678, 3824, 4072, 4116, 4178, 4240,4374, 4412, 4462, 4576, 4664, 4856, 5178, 5286, 5362, 5662, 5682, 5984,6018, 6132, 6256, 6286, 6532, 6714, 6754, 6764, 6790, 6938, 7144, 7442,7674, 7872, 8278, 8498, 8564, 8782, 8784, 9276 and 9580. SEQ ID NOs 5662(ORF02831) and 8564 (ORF04282) are preferred lipoproteins.

Preferred polypeptides are those listed in Table 2.

The invention further provides polypeptides comprising fragments of theamino acid sequences disclosed in the examples. Similarly, the inventionprovides polypeptides comprising fragments of the amino acid sequencesencoded within SEQ ID NOs 9991 to 10273 or within SEQ ID NO: 10274. Thefragments should comprise at least n consecutive amino acids from thesequences and, depending on the particular sequence, n is 7 or more(e.g. 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 35, 40, 45, 50, 60,70, 80, 90, 100 or more). The fragment may comprise at least one T-cellor, preferably, a B-cell epitope of the sequence. T- and B-cell epitopescan be identified empirically (e.g. using PEPSCAN [14,15] or similarmethods), or they can be predicted (e.g. using the Jameson-Wolfantigenic index [16], matrix-based approaches [17], TEPITOPE [18],neural networks [19], OptiMer & EpiMer [20,21], ADEPT [22], Tsites [23],hydrophilicity [24], antigenic index [25] or the methods disclosed inreference 26, etc.). Other preferred fragments are (a) the N-terminalsignal peptides of the polypeptides of the invention, (b) thepolypeptides, but without their N-terminal signal peptides, (c) thepolypeptides, but without 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 of theirN-terminal amino acid residue(s).

Other preferred fragments are those that begin with an amino acidencoded by a potential start codon (ATG, GTG, TTG). Fragments startingat the methionine encoded by a start codon downstream of the indicatedstart codon are polypeptides of the invention.

Other preferred fragments are those that are common to a polypeptide ofthe invention and to a polypeptide identified in any of references 5, 6,8, 10 and 11.

Polypeptides of the invention can be prepared in many ways e.g. bychemical synthesis (in whole or in part), by digesting longerpolypeptides using proteases, by translation from RNA, by purificationfrom cell culture (e.g. from recombinant expression), from the organismitself (e.g. after bacterial culture, or direct from patients), etc. Apreferred method for production of peptides <40 amino acids longinvolves in vitro chemical synthesis [27,28]. Solid-phase peptidesynthesis is particularly preferred, such as methods based on tBoc orFmoc [29] chemistry. Enzymatic synthesis [30] may also be used in partor in full. As an alternative to chemical synthesis, biologicalsynthesis may be used e.g. the polypeptides may be produced bytranslation. This may be carried out in vitro or in vivo. Biologicalmethods are in general restricted to the production of polypeptidesbased on L-amino acids, but manipulation of translation machinery (e.g.of aminoacyl tRNA molecules) can be used to allow the introduction ofD-amino acids (or of other non natural amino acids, such as iodotyrosineor methylphenylalanine, azidohomoalanine, etc.) [31]. Where D-aminoacids are included, however, it is preferred to use chemical synthesis.Polypeptides of the invention may have covalent modifications at theC-terminus and/or N-terminus.

Polypeptides of the invention can take various forms (e.g. native,fusions, glycosylated, non-glycosylated, lipidated, non-lipidated,phosphorylated, non-phosphorylated, myristoylated, non-myristoylated,monomeric, multimeric, particulate, denatured, etc.).

Polypeptides of the invention are preferably provided in purified orsubstantially purified form i.e. substantially free from otherpolypeptides (e.g. free from naturally-occurring polypeptides),particularly from other ExPEC or host cell polypeptides, and aregenerally at least about 50% pure (by weight), and usually at leastabout 90% pure i.e. less than about 50%, and more preferably less thanabout 10% (e.g. 5% or less) of a composition is made up of otherexpressed polypeptides. Polypeptides of the invention are preferablyExPEC, and more preferably MNEC, polypeptides.

Polypeptides of the invention may be attached to a solid support.Polypeptides of the invention may comprise a detectable label (e.g. aradioactive or fluorescent label, or a biotin label).

The term “polypeptide” refers to amino acid polymers of any length. Thepolymer may be linear or branched, it may comprise modified amino acids,and it may be interrupted by non-amino acids. The terms also encompassan amino acid polymer that has been modified naturally or byintervention; for example, disulfide bond formation, glycosylation,lipidation, acetylation, phosphorylation, or any other manipulation ormodification, such as conjugation with a labeling component. Alsoincluded within the definition are, for example, polypeptides containingone or more analogs of an amino acid (including, for example, unnaturalamino acids, etc.), as well as other modifications known in the art.Polypeptides can occur as single chains or associated chains.Polypeptides of the invention can be naturally or non-naturallyglycosylated (i.e. the polypeptide has a glycosylation pattern thatdiffers from the glycosylation pattern found in the correspondingnaturally occurring polypeptide).

Polypeptides of the invention may be at least 40 amino acids long (e.g.at least 40, 50, 60, 70, 80, 90, 100, 120, 140, 160, 180, 200, 220, 240,260, 280, 300, 350, 400, 450, 500 or more). Polypeptides of theinvention may be shorter than 500 amino acids (e.g. no longer than 40,50, 60, 70, 80, 90, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280,300, 350, 400 or 450 amino acids).

The invention provides polypeptides comprising a sequence —X—Y— or—Y—X—, wherein: —X— is an amino acid sequence as defined above and —Y—is not a sequence as defined above i.e. the invention provides fusionproteins. Where the N-terminus codon of a polypeptide-coding sequence isnot ATG then that codon will be translated as the standard amino acidfor that codon rather than as a Met, which occurs when the codon istranslated as a start codon.

The invention provides a process for producing polypeptides of theinvention, comprising the step of culturing a host cell of to theinvention under conditions which induce polypeptide expression.

The invention provides a process for producing a polypeptide of theinvention, wherein the polypeptide is synthesised in part or in wholeusing chemical means.

The invention provides a composition comprising two or more (e.g. 2, 3,4, 5, 6 or more) polypeptides of the invention. The differentpolypeptides may be selected such that they are involved in differentbacterial metabolic and/or signalling pathways e.g. selection from 2, 3,4, 5, 6 or more of the following categories: adhesins; autotransporterproteins; toxins; iron acquisitions proteins; and membrane-associatedproteins, including in particular integral outer membrane proteins. Suchcombinations of antigens may target the immune response againstdifferent aspects of the bacterial life cycle, resulting in a moreeffective immune response.

The invention also provides a hybrid polypeptide represented by theformula NH₂-A-[-X-L-]-B—COOH, wherein X is a polypeptide of theinvention as defined above, L is an optional linker amino acid sequence,A is an optional N-terminal amino acid sequence, B is an optionalC-terminal amino acid sequence, and n is an integer greater than 1. Thevalue of n is between 2 and x, and the value of x is typically 3, 4, 5,6, 7, 8, 9 or 10. Preferably n is 2, 3 or 4; it is more preferably 2 or3; most preferably, n=2. For each n instances, —X— may be the same ordifferent. For each n instances of [-X-L-], linker amino acid sequence-L- may be present or absent. For instance, when n=2 the hybrid may beNH₂—X₁-L₁-X₂-L₂-COOH, NH₂—X₁—X₂—COOH, NH₂—X₁-L₁-X₂—COOH,NH₂—X₁—X₂-L₂-COOH, etc. Linker amino acid sequence(s) -L- will typicallybe short (e.g. 20 or fewer amino acids i.e. 19, 18, 17, 16, 15, 14, 13,12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1). Examples include short peptidesequences which facilitate cloning, poly-glycine linkers (i.e. Gly_(n)where n=2, 3, 4, 5, 6, 7, 8, 9, 10 or more), and histidine tags (i.e.His where n=3, 4, 5, 6, 7, 8, 9, 10 or more). Other suitable linkeramino acid sequences will be apparent to those skilled in the art. -A-and —B— are optional sequences which will typically be short (e.g. 40 orfewer amino acids i.e. 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28,27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, 1). Examples include leader sequences to directpolypeptide trafficking, or short peptide sequences which facilitatecloning or purification (e.g. histidine tags i.e. His where n=3, 4, 5,6, 7, 8, 9, 10 or more). Other suitable N-terminal and C-terminal aminoacid sequences will be apparent to those skilled in the art.

Various tests can be used to assess the in vivo immunogenicity ofpolypeptides of the invention. For example, polypeptides can beexpressed recombinantly and used to screen patient sera by immunoblot. Apositive reaction between the polypeptide and patient serum indicatesthat the patient has previously mounted an immune response to theprotein in question i.e. the protein is an immunogen. This method canalso be used to identify immunodominant proteins.

Antibodies

The invention provides antibodies that bind to polypeptides of theinvention. These may be polyclonal or monoclonal and may be produced byany suitable means (e.g. by recombinant expression). To increasecompatibility with the human immune system, the antibodies may bechimeric or humanised [e.g. refs. 32 & 33], or fully human antibodiesmay be used. The antibodies may include a detectable label (e.g. fordiagnostic assays). Antibodies of the invention may be attached to asolid support. Antibodies of the invention are preferably neutralisingantibodies.

Monoclonal antibodies are particularly useful in identification andpurification of the individual polypeptides against which they aredirected. Monoclonal antibodies of the invention may also be employed asreagents in immunoassays, radioimmunoassays (RIA) or enzyme-linkedimmunosorbent assays (ELISA), etc. In these applications, the antibodiescan be labelled with an analytically-detectable reagent such as aradioisotope, a fluorescent molecule or an enzyme. The monoclonalantibodies produced by the above method may also be used for themolecular identification and characterization (epitope mapping) ofpolypeptides of the invention.

Antibodies of the invention are preferably specific to ExPEC strains ofE. coli, i.e. they bind preferentially to ExPEC E. coli relative toother bacteria (e.g. relative to non-ExPEC E. coli and relative tonon-E. coli bacteria). More preferably, the antibodies are specific toMNEC strains i.e. they bind preferentially to MNEC bacteria relative toother bacteria, including relative to other ExPEC E. coli.

Antibodies of the invention are preferably provided in purified orsubstantially purified form. Typically, the antibody will be present ina composition that is substantially free of other polypeptides e.g.where less than 90% (by weight), usually less than 60% and more usuallyless than 50% of the composition is made up of other polypeptides.

Antibodies of the invention can be of any isotype (e.g. IgA, IgG, IgMi.e. an α, γ or μ heavy chain), but will generally be IgG. Within theIgG isotype, antibodies may be IgG1, IgG2, IgG3 or IgG4 subclass.Antibodies of the invention may have a κ or a λ light chain.

Antibodies of the invention can take various forms, including wholeantibodies, antibody fragments such as F(ab′)₂ and F(ab) fragments, Fvfragments (non-covalent heterodimers), single-chain antibodies such assingle chain Fv molecules (scFv), minibodies, oligobodies, etc. The term“antibody” does not imply any particular origin, and includes antibodiesobtained through non-conventional processes, such as phage display.

The invention provides a process for detecting polypeptides of theinvention, comprising the steps of: (a) contacting an antibody of theinvention with a biological sample under conditions suitable for theformation of an antibody-antigen complexes; and (b) detecting saidcomplexes.

The invention provides a process for detecting antibodies of theinvention, comprising the steps of: (a) contacting a polypeptide of theinvention with a biological sample (e.g. a blood or serum sample) underconditions suitable for the formation of an antibody-antigen complexes;and (b) detecting said complexes.

Preferred antibodies bind to a polypeptide of the invention withsubstantially greater affinity than antibodies known in the art.Preferably, the affinity is at least 1.5-fold, 2-fold, 5-fold 10-fold,100-fold, 10³-fold, 10⁴-fold, 10⁵-fold, 10⁶-fold etc. stronger thanantibodies known in the art.

Nucleic Acids

The invention also provides nucleic acid comprising a nucleotidesequence encoding the polypeptides of the invention (e.g. theodd-numbered SEQ ID NOs: 1 to 9989). The invention also provides nucleicacid comprising any one of SEQ ID NOs 9991 to 10273.

The invention also provides nucleic acid comprising nucleotide sequenceshaving sequence identity to such nucleotide sequences. Identity betweensequences is preferably determined by the Smith-Waterman homology searchalgorithm as described above. Depending on the particular sequence, thedegree of sequence identity is preferably greater than 50% (e.g. 60%,70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% ormore).

The invention also provides nucleic acid which can hybridize to thesenucleic acids. Hybridization reactions can be performed under conditionsof different “stringency”. Conditions that increase stringency of ahybridization reaction of widely known and published in the art [e.g.page 7.52 of reference 34]. Examples of relevant conditions include (inorder of increasing stringency): incubation temperatures of 25° C., 37°C., 50° C., 55° C. and 68° C.; buffer concentrations of 10×SSC, 6×SSC,1×SSC, 0.1×SSC (where SSC is 0.15 M NaCl and 15 mM citrate buffer) andtheir equivalents using other buffer systems; formamide concentrationsof 0%, 25%, 50%, and 75%; incubation times from 5 minutes to 24 hours;1, 2, or more washing steps; wash incubation times of 1, 2, or 15minutes; and wash solutions of 6×SSC, 1×SSC, 0.1×SSC, or de-ionizedwater. Hybridization techniques and their optimization are well known inthe art [e.g. see refs 34-37, etc.].

In some embodiments, nucleic acid of the invention hybridizes to atarget under low stringency conditions; in other embodiments ithybridizes under intermediate stringency conditions; in preferredembodiments, it hybridizes under high stringency conditions. Anexemplary set of low stringency hybridization conditions is 50° C. and10×SSC. An exemplary set of intermediate stringency hybridizationconditions is 55° C. and 1×SSC. An exemplary set of high stringencyhybridization conditions is 68° C. and 0.1×SSC.

Nucleic acid comprising fragments of these sequences are also provided.These should comprise at least n consecutive nucleotides from thesequences and, depending on the particular sequence, n is 10 or more(e.g. 12, 14, 15, 18, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100, 150,200 or more). Preferred fragments are those that are common to a nucleicacid sequence of the invention and to a nucleic acid sequence identifiedin any of references 5, 6, 8, 10 and 11.

The invention provides nucleic acid of formula 5′-X—Y—Z-3′, wherein: —X—is a nucleotide sequence consisting of x nucleotides; —Z— is anucleotide sequence consisting of z nucleotides; —Y— is a nucleotidesequence consisting of either (a) a fragment of a nucleic acid sequenceselected from odd-numbered SEQ ID NOS: 1 to 9989, (b) a fragment of anyone of SEQ ID NOs 9991 to 10273; (c) a fragment of SEQ ID NO: 10274; or(d) the complement of (a) or (b) or (c); and said nucleic acid5′-X—Y—Z-3′ is neither (i) a fragment of either an odd-numbered nucleicacid from SEQ ID NOS: 1 to 9989 or of SEQ ID NOs 9991 to 10273 or of SEQID NO: 10274, nor (ii) the complement of (i). The —X— and/or —Z—moieties may comprise a promoter sequence (or its complement).

The invention also provides nucleic acid comprising a fragment of atleast n consecutive nucleotides from SEQ ID 10274, wherein n is 10 ormore e.g. 12, 14, 15, 18, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100,150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 800, 900, 1000, 1500,2000, 3000, 4000, 5000, 10000, 100000, 1000000 or more. This nucleicacid may be single-stranded, at least in part, such that it can act as ahybridisation probe and/or amplification primer. In some embodiments,the fragment is not, or does not include, a fragment of a prior artExPEC sequence e.g. not a fragment of a nucleic acid sequencespecifically disclosed in any of refs. 5, 6, 7, 8, 9, 10, 11 and 13. Inother embodiments, the fragment is also a fragment of a prior art ExPECsequence e.g. is identical to a fragment of a nucleic acid sequencespecifically disclosed in any of refs. 5, 6, 7, 8, 9, 10, 11 and 13.

The invention includes nucleic acid comprising sequences complementaryto these sequences (e.g. for antisense or probing, or for use asprimers).

Nucleic acids of the invention can be used in hybridisation reactions(e.g. Northern or Southern blots, or in nucleic acid microarrays or‘gene chips’) and amplification reactions (e.g. PCR, SDA, SSSR, LCR,TMA, NASBA, etc.) and other nucleic acid techniques.

Nucleic acid according to the invention can take various forms (e.g.single-stranded, double-stranded, vectors, primers, probes, labelledetc.). Nucleic acids of the invention may be circular or branched, butwill generally be linear. Unless otherwise specified or required, anyembodiment of the invention that utilizes a nucleic acid may utilizeboth the double-stranded form and each of two complementarysingle-stranded forms which make up the double-stranded form. Primersand probes are generally single-stranded, as are antisense nucleicacids.

Nucleic acids of the invention are preferably provided in purified orsubstantially purified form i.e. substantially free from other nucleicacids (e.g. free from naturally-occurring nucleic acids), particularlyfrom other ExPEC or host cell nucleic acids, generally being at leastabout 50% pure (by weight), and usually at least about 90% pure. Nucleicacids of the invention are preferably ExPEC nucleic acids.

Nucleic acids of the invention may be prepared in many ways e.g. bychemical synthesis (e.g. phosphoramidite synthesis of DNA) in whole orin part, by digesting longer nucleic acids using nucleases (e.g.restriction enzymes), by joining shorter nucleic acids or nucleotides(e.g. using ligases or polymerases), from genomic or cDNA libraries,etc.

Nucleic acid of the invention may be attached to a solid support (e.g. abead, plate, filter, film, slide, microarray support, resin, etc.).Nucleic acid of the invention may be labelled e.g. with a radioactive orfluorescent label, or a biotin label. This is particularly useful wherethe nucleic acid is to be used in detection techniques e.g. where thenucleic acid is a primer or as a probe.

The term “nucleic acid” includes in general means a polymeric form ofnucleotides of any length, which contain deoxyribonucleotides,ribonucleotides, and/or their analogs. It includes DNA, RNA, DNA/RNAhybrids. It also includes DNA or RNA analogs, such as those containingmodified backbones (e.g. peptide nucleic acids (PNAs) orphosphorothioates) or modified bases. Thus the invention includes mRNA,tRNA, rRNA, ribozymes, DNA, cDNA, recombinant nucleic acids, branchednucleic acids, plasmids, vectors, probes, primers, etc. Where nucleicacid of the invention takes the form of RNA, it may or may not have a 5′cap.

Nucleic acids of the invention comprise sequences, but they may alsocomprise non-ExPEC sequences (e.g. in nucleic acids of formula5′-X—Y—Z-3′, as defined above). This is particularly useful for primers,which may thus comprise a first sequence complementary to a nucleic acidtarget and a second sequence which is not complementary to the nucleicacid target. Any such non-complementary sequences in the primer arepreferably 5′ to the complementary sequences. Typical non-complementarysequences comprise restriction sites or promoter sequences.

Nucleic acids of the invention can be prepared in many ways e.g. bychemical synthesis (at least in part), by digesting longer nucleic acidsusing nucleases (e.g. restriction enzymes), by joining shorter nucleicacids (e.g. using ligases or polymerases), from genomic or cDNAlibraries, etc.

Nucleic acids of the invention may be part of a vector i.e. part of anucleic acid construct designed for transduction/transfection of one ormore cell types. Vectors may be, for example, “cloning vectors” whichare designed for isolation, propagation and replication of insertednucleotides, “expression vectors” which are designed for expression of anucleotide sequence in a host cell, “viral vectors” which is designed toresult in the production of a recombinant virus or virus-like particle,or “shuttle vectors”, which comprise the attributes of more than onetype of vector. Preferred vectors are plasmids. A “host cell” includesan individual cell or cell culture which can be or has been a recipientof exogenous nucleic acid. Host cells include progeny of a single hostcell, and the progeny may not necessarily be completely identical (inmorphology or in total DNA complement) to the original parent cell dueto natural, accidental, or deliberate mutation and/or change. Host cellsinclude cells transfected or infected in vivo or in vitro with nucleicacid of the invention.

Where a nucleic acid is DNA, it will be appreciated that “U” in a RNAsequence will be replaced by “T” in the DNA. Similarly, where a nucleicacid is RNA, it will be appreciated that “T” in a DNA sequence will bereplaced by “U” in the RNA.

The term “complement” or “complementary” when used in relation tonucleic acids refers to Watson-Crick base pairing. Thus the complementof C is G, the complement of G is C, the complement of A is T (or U),and the complement of T (or U) is A. It is also possible to use basessuch as I (the purine inosine) e.g. to complement pyrimidines (C or T).The terms also imply a direction—the complement of 5′-ACAGT-3′ is5′-ACTGT-3′ rather than 5′-TGTCA-3′.

Nucleic acids of the invention can be used, for example: to producepolypeptides; as hybridization probes for the detection of nucleic acidin biological samples; to generate additional copies of the nucleicacids; to generate ribozymes or antisense oligonucleotides; assingle-stranded DNA primers or probes; or as triple-strand formingoligonucleotides.

The invention provides a process for producing nucleic acid of theinvention, wherein the nucleic acid is synthesised in part or in wholeusing chemical means.

The invention provides vectors comprising nucleotide sequences of theinvention (e.g. cloning or expression vectors) and host cellstransformed with such vectors.

The invention also provides a kit comprising primers (e.g. PCR primers)for amplifying a template sequence contained within an ExPEC nucleicacid sequence, the kit comprising a first primer and a second primer,wherein the first primer is substantially complementary to said templatesequence and the second primer is substantially complementary to acomplement of said template sequence, wherein the parts of said primerswhich have substantial complementarity define the termini of thetemplate sequence to be amplified. The first primer and/or the secondprimer may include a detectable label (e.g. a fluorescent label).

The invention also provides a kit comprising first and secondsingle-stranded oligonucleotides which allow amplification of a ExPECtemplate nucleic acid sequence contained in a single- or double-strandednucleic acid (or mixture thereof), wherein: (a) the firstoligonucleotide comprises a primer sequence which is substantiallycomplementary to said template nucleic acid sequence; (b) the secondoligonucleotide comprises a primer sequence which is substantiallycomplementary to the complement of said template nucleic acid sequence;(c) the first oligonucleotide and/or the second oligonucleotidecomprise(s) sequence which is not complementary to said template nucleicacid; and (d) said primer sequences define the termini of the templatesequence to be amplified. The non-complementary sequence(s) of feature(c) are preferably upstream of (i.e. 5′ to) the primer sequences. One orboth of these (c) sequences may comprise a restriction site [e.g. ref.38] or a promoter sequence [e.g. 39]. The first oligonucleotide and/orthe second oligonucleotide may include a detectable label (e.g. afluorescent label).

The template sequence may be any part of a genome sequence (e.g. SEQ IDNO:10274). For example, it could be a rRNA gene (e.g. Turenne et al.(2000) J. Clin. Microbiol. 38:513-520) or a protein-coding gene. Thetemplate sequence is preferably specific to ExPEC, and more preferablyto MNEC. The invention also provides a computer-readable medium (e.g. afloppy disk, a hard disk, a CD-ROM, a DVD etc.) and/or a computerdatabase containing one or more of the sequences in the sequencelisting. The medium preferably contains SEQ ID NO: 10274.

The invention provides a process for detecting nucleic acid of theinvention, comprising the steps of: (a) contacting a nucleic probeaccording to the invention with a biological sample under hybridisingconditions to form duplexes; and (b) detecting said duplexes.

The invention provides a process for detecting in a biological sample(e.g. blood), comprising the step of contacting nucleic acid accordingto the invention with the biological sample under hybridisingconditions. The process may involve nucleic acid amplification (e.g.PCR, SDA, SSSR, LCR, TMA, NASBA, etc.) or hybridisation (e.g.microarrays, blots, hybridisation with a probe in solution etc.). PCRdetection of ExPEC in clinical samples has been reported [e.g. see ref.40]. Clinical assays based on nucleic acid are described in general inref. 41.

The invention provides a process for preparing a fragment of a targetsequence, wherein the fragment is prepared by extension of a nucleicacid primer. The target sequence and/or the primer are nucleic acids ofthe invention. The primer extension reaction may involve nucleic acidamplification (e.g. PCR, SDA, SSSR, LCR, TMA, NASBA, etc.).

Nucleic acid amplification according to the invention may bequantitative and/or real-time.

For certain embodiments of the invention, nucleic acids are preferablyat least 7 nucleotides in length (e.g. 8, 9, 10, 11, 12, 13, 14, 15, 16,17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,35, 36, 37, 38, 39, 40, 45, 50, 55, 60, 65, 70, 75, 80, 90, 100, 110,120, 130, 140, 150, 160, 170, 180, 190, 200, 225, 250, 275, 300nucleotides or longer).

For certain embodiments of the invention, nucleic acids are preferablyat most 500 nucleotides in length (e.g. 450, 400, 350, 300, 250, 200,150, 140, 130, 120, 110, 100, 90, 80, 75, 70, 65, 60, 55, 50, 45, 40,39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22,21, 20, 19, 18, 17, 16, 15 nucleotides or shorter).

Primers and probes of the invention, and other nucleic acids used forhybridization, are preferably between 10 and 30 nucleotides in length(e.g. 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, or 30 nucleotides).

Mutant Bacteria

The invention provides a E. coli bacterium in which expression of one ormore of the genes identified herein has been knocked out. Techniques forgene knockout are well known, and knockout mutants of E. coli have beenreported previously.

The knockout is preferably achieved using isogenic deletion of thecoding region, but any other suitable technique may be used e.g.deletion or mutation of the promoter, deletion or mutation of the startcodon, antisense inhibition, inhibitory RNA, etc. In the resultingbacterium, however, mRNA encoding the gene product will be absent and/orits translation will be inhibited (e.g. to less than 1% of wild-typelevels).

The bacterium may contain a marker gene in place of the knocked out genee.g. an antibiotic resistance marker.

Vesicles

Reference 42 describes the preparation of vesicles from a MNEC strain bythe knockout of mltA (a murein lytic transglycosylase) or one or more ofthe components of the E. coli Tol-Pal complex [43], such as tolA, tolQ,tolB, pal and/or tolR. These vesicles can be improved by making one ormore further genetic changes to the chromosome of the bacterium orthrough insertion of “ad-hoc” episomalic elements (e.g. expressionvectors) in order to increase the amount of and/or immunoaccessibility“exposure” of protective antigens on the surface the vesicles.

One way of obtaining such improvements is to up-regulate the expressionof the polypeptides protective antigens of the invention. Many differentgenetic strategies for increasing the expression of a target protein arewell-known in the art and can be distinguished into two broadcategories: one relying on modifications of the chromosome (e.g.replacement of the wild-type promoter with a stronger promoter,inactivation of natural repressor genes, etc.) to increase expression ofan endogenous gene, and the other based on recombinant expression byepisomalic elements (e.g. high-copy number plasmids, vectors harboringan engineered target gene, etc.) or integration of a exogenousprotective target gene in the chromosome. Practical examples for each ofthese approaches can be found in references 44 to 50.

Another way of increasing vesicle immunogenicity and selectivity is todown-regulate the expression of immunodominant non-protective antigensor to down-regulate proteins that are homologous to proteins found incommensal strains. Further improvements can be achieved sought byreducing the potential vesicle toxicity through detoxification of theLipid A moiety of LPS. Similar changes have been previously described toproduce improved vesicles from other Gram-negative pathogens (see forexample references 51 & 52).

All the above strategies can be used either alone or in combination toobtain improved vesicles for use in immunogenic compositions. Theinvention provides a pathogenic Escherichia coli bacterium (particularlya MNEC) having a knockout of mltA and/or of a component of its Tol-Palcomplex, and one or more of: (i) a chromosomal gene encoding apolypeptide of the invention under the control of a promoter thatprovides higher expression levels of the polypeptide than the promoterthat is naturally associated with the gene encoding the polypeptide;(ii) an autonomously-replicating extrachromosomal element encoding apolypeptide of the invention; and/or (iii) a genetic modification toreduce the toxicity of the Lipid A moiety of E. coli LPS relative towild-type LPS.

The invention also provides vesicles obtainable by culturing such abacterium, such as the vesicles that, during culture of the bacterium,are released into the culture medium.

Pharmaceutical Compositions

The invention provides compositions comprising: (a) polypeptide,antibody, vesicles, and/or nucleic acid of the invention; and (b) apharmaceutically acceptable carrier. These compositions may be suitableas immunogenic compositions, for instance, or as diagnostic reagents, oras vaccines. Vaccines according to the invention may either beprophylactic (i.e. to prevent infection) or therapeutic (i.e. to treatinfection), but will typically be prophylactic.

In a particular aspect, the invention provides immunogenic compositionscomprising one or more outer membrane vesicles (OMVs) expressing oroverexpressing one or more polypeptides encoded by: (a) a nucleotidesequence selected from the group consisting of SEQ ID NOs 511, 2453,2455, 2677, 2691, 4595, 4747, 7003, 7051, 8167, 4627 and 5699; (b) anucleotide sequence having at least 80% sequence identity to an aminoacid sequence of (a); (c) a nucleotide sequence which is a fragment ofat least 10 consecutive nucleotides from a nucleotide sequence of (a);or (d) a nucleotide sequence having at least 80% sequence identity to anucleotide sequence of (a) and including a fragment of at least 10consecutive nucleotides from a nucleotide sequence of (a).

In another aspect, the invention provides immunogenic compositionscomprising one or more outer membrane vesicles (OMVs) expressing oroverexpressing one or more polypeptides encoded by: (a) a nucleotidesequence selected from the group consisting of SEQ ID Nos 8563, 2727,9871, 4671, 5679, 533, 3221, 3225 and 7003; (b) a nucleotide sequencehaving at least 80% sequence identity to an amino acid sequence of (a);(c) a nucleotide sequence which is a fragment of at least 10 consecutivenucleotides from a nucleotide sequence of (a); or (d) a nucleotidesequence having at least 80% sequence identity to a nucleotide sequenceof (a) and including a fragment of at least 10 consecutive nucleotidesfrom a nucleotide sequence of (a).

A ‘pharmaceutically acceptable carrier’ includes any carrier that doesnot itself induce the production of antibodies harmful to the individualreceiving the composition. Suitable carriers are typically large, slowlymetabolised macromolecules such as proteins, polysaccharides, polylacticacids, polyglycolic acids, polymeric amino acids, amino acid copolymers,sucrose, trehalose, lactose, and lipid aggregates (such as oil dropletsor liposomes). Such carriers are well known to those of ordinary skillin the art. The vaccines may also contain diluents, such as water,saline, glycerol, etc.

Additionally, auxiliary substances, such as wetting or emulsifyingagents, pH buffering substances, and the like, may be present. Sterilepyrogen-free, phosphate-buffered physiologic saline is a typicalcarrier. A thorough discussion of pharmaceutically acceptable excipientsis available in ref. 289.

Compositions of the invention may include an antimicrobial, particularlyif packaged in a multiple dose format.

Compositions of the invention may comprise detergent e.g. a Tween(polysorbate), such as Tween 80. Detergents are generally present at lowlevels e.g. <0.01%.

Compositions of the invention may include sodium salts (e.g. sodiumchloride) to give tonicity. A concentration of 10+2 mg/ml NaCl istypical.

Compositions of the invention will generally include a buffer. Aphosphate buffer is typical.

Compositions of the invention may comprise a sugar alcohol (e.g.mannitol) or a disaccharide (e.g. sucrose or trehalose) e.g. at around15-30 mg/ml (e.g. 25 mg/ml), particularly if they are to be lyophilisedor if they include material which has been reconstituted fromlyophilised material. The pH of a composition for lyophilisation may beadjusted to around 6.1 prior to lyophilisation.

Polypeptides of the invention may be administered in conjunction withother immunoregulatory agents. In particular, compositions will usuallyinclude a vaccine adjuvant. The adjuvant may be selected from one ormore of the group consisting of a TH1 adjuvant and TH2 adjuvant, furtherdiscussed below. Adjuvants which may be used in compositions of theinvention include, but are not limited to:

A. Mineral-Containing Compositions

Mineral containing compositions suitable for use as adjuvants in theinvention include mineral salts, such as aluminium salts and calciumsalts. The invention includes mineral salts such as hydroxides (e.g.oxyhydroxides), phosphates (e.g. hydroxyphosphates, orthophosphates),sulphates, etc. [e.g. see chapters 8 & 9 of ref. 53], or mixtures ofdifferent mineral compounds (e.g. a mixture of a phosphate and ahydroxide adjuvant, optionally with an excess of the phosphate), withthe compounds taking any suitable form (e.g. gel, crystalline,amorphous, etc.), and with adsorption to the salt(s) being preferred.Mineral containing compositions may also be formulated as a particle ofmetal salt [54].

Aluminum salts may be included in vaccines of the invention such thatthe dose of Al³⁺ is between 0.2 and 1.0 mg per dose.

In another embodiment the adjuvant of the invention comprises bothaluminum phosphate and aluminum hydroxide. In a more particularembodiment thereof, the adjuvant has a greater amount of aluminumphosphate than aluminum hydroxide, such as a ratio of 2:1, 3:1, 4:1,5:1, 6:1, 7:1, 8:1, 9:1 or greater than 9:1, by weight aluminumphosphate to aluminum hydroxide. More particular still, aluminum saltsin the vaccine are present at 0.4 to 1.0 mg per vaccine dose, or 0.4 to0.8 mg per vaccine dose, or 0.5 to 0.7 mg per vaccine dose, or about 0.6mg per vaccine dose.

Generally, the preferred aluminum-based adjuvant(s), or ratio ofmultiple aluminum-based adjuvants, such as aluminum phosphate toaluminum hydroxide is selected by optimization of electrostaticattraction between molecules such that the antigen carries an oppositecharge as the adjuvant at the desired pH. For example, an aluminumphosphate adjuvant with pI ˜4 electrostatically adsorbs lysozyme, butnot albumin, at pH 7.4. Should albumin be the target, aluminum hydroxideadjuvant would be selected (e.g. with pH 11.4). Alternatively,pre-treatment of aluminum hydroxide with phosphate lowers its pI, makingit a preferred adjuvant for more basic antigens.

A typical aluminium phosphate adjuvant is amorphous aluminiumhydroxyphosphate with PO₄/Al molar ratio between 0.84 and 0.92, includedat 0.6 mg Al³⁺/ml. Adsorption with a low dose of aluminium phosphate maybe used e.g. between 50 and 100 μg Al³⁺ per conjugate per dose. Where analuminium phosphate it used and it is desired not to adsorb an antigento the adjuvant, this is favoured by including free phosphate ions insolution (e.g. by the use of a phosphate buffer).

B. Oil Emulsions

Oil emulsion compositions suitable for use as adjuvants in the inventioninclude squalene-water emulsions, such as MF59 (5% Squalene, 0.5% Tween80, and 0.5% Span 85, formulated into submicron particles using amicrofluidizer) [Chapter 10 of ref. 53; see also refs. 55-57 chapter 12of ref. 58.]. MF59 is used as the adjuvant in the FLUAD™ influenza virustrivalent subunit vaccine. The emulsion advantageously includes citrateions e.g. 10 mM sodium citrate buffer.

Particularly preferred adjuvants for use in the compositions aresubmicron oil-in-water emulsions. Preferred submicron oil-in-wateremulsions for use herein are squalene/water emulsions, optionallycontaining varying amounts of MTP-PE, such as a submicron oil-in-wateremulsion containing 4-5% w/v squalene, 0.25-1.0% w/v Tween 80(polyoxyelthylenesorbitan monooleate), and/or 0.25-1.0% Span 85(sorbitan trioleate), and, optionally,N-acetylmuramyl-L-alanyl-D-isogluatminyl-L-alanine-2-(1′-2′-dipalmitoyl-sn-glycero-3-hydroxyphosphophoryloxy)-ethylamine(MTP-PE). Submicron oil-in-water emulsions, methods of making the sameand immunostimulating agents, such as muramyl peptides, for use in thecompositions, are described in detail in references 55 & 59-60.

An emulsion of squalene, a tocopherol, and Tween 80 can be used. Theemulsion may include phosphate buffered saline. It may also include Span85 (e.g. at 1%) and/or lecithin. These emulsions may have from 2 to 10%squalene, from 2 to 10% tocopherol and from 0.3 to 3% Tween 80, and theweight ratio of squalene:tocopherol is preferably <1 as this provides amore stable emulsion. One such emulsion can be made by dissolving Tween80 in PBS to give a 2% solution, then mixing 90 ml of this solution witha mixture of (5 g of DL-α-tocopherol and 5 ml squalene), thenmicrofluidising the mixture. The resulting emulsion may have submicronoil droplets e.g. with an average diameter of between 100 and 250 nm,preferably about 180 nm.

An emulsion of squalene, a tocopherol, and a Triton detergent (e.g.Triton X-100) can be used.

An emulsion of squalane, polysorbate 80 and poloxamer 401 (“Pluronic™L121”) can be used. The emulsion can be formulated in phosphate bufferedsaline, pH 7.4. This emulsion is a useful delivery vehicle for muramyldipeptides, and has been used with threonyl-MDP in the “SAF-1” adjuvant[61] (0.05-1% Thr-MDP, 5% squalane, 2.5% Pluronic L121 and 0.2%polysorbate 80). It can also be used without the Thr-MDP, as in the “AF”adjuvant [62] (5% squalane, 1.25% Pluronic L121 and 0.2% polysorbate80). Microfluidisation is preferred.

Complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA)may also be used as adjuvants in the invention.

C. Saponin Formulations [Chapter 22 of Ref 53]

Saponin formulations may also be used as adjuvants in the invention.Saponins are a heterologous group of sterol glycosides and triterpenoidglycosides that are found in the bark, leaves, stems, roots and evenflowers of a wide range of plant species. Saponins isolated from thebark of the Quillaia saponaria Molina tree have been widely studied asadjuvants. Saponin can also be commercially obtained from Smilax omata(sarsaprilla), Gypsophilla paniculata (brides veil), and Saponariaofficianalis (soap root). Saponin adjuvant formulations include purifiedformulations, such as QS21, as well as lipid formulations, such asISCOMs.

Saponin compositions have been purified using HPLC and RP-HPLC. Specificpurified fractions using these techniques have been identified,including QS7, QS17, QS18, QS21, QH-A, QH-B and QH-C. Preferably, thesaponin is QS21. A method of production of QS21 is disclosed in ref. 63.Saponin formulations may also comprise a sterol, such as cholesterol[64].

Combinations of saponins and cholesterols can be used to form uniqueparticles called immunostimulating complexs (ISCOMs) [chapter 23 of ref.53]. ISCOMs typically also include a phospholipid such asphosphatidylethanolamine or phosphatidylcholine. Any known saponin canbe used in ISCOMs. Preferably, the ISCOM includes one or more of QuilA,QHA and QHC. ISCOMs are further described in refs. 64-66. Optionally,the ISCOMS may be devoid of additional detergent(s) [67].

A review of the development of saponin based adjuvants can be found inrefs. 68 & 69.

D. Virosomes and Virus-Like Particles

Virosomes and virus-like particles (VLPs) can also be used as adjuvantsin the invention. These structures generally contain one or moreproteins from a virus optionally combined or formulated with aphospholipid. They are generally non-pathogenic, non-replicating andgenerally do not contain any of the native viral genome. The viralproteins may be recombinantly produced or isolated from whole viruses.These viral proteins suitable for use in virosomes or VLPs includeproteins derived from influenza virus (such as HA or NA), Hepatitis Bvirus (such as core or capsid proteins), Hepatitis E virus, measlesvirus, Sindbis virus, Rotavirus, Foot-and-Mouth Disease virus,Retrovirus, Norwalk virus, human Papilloma virus, HIV, RNA-phages,Qβ-phage (such as coat proteins), GA-phage, fr-phage, AP205 phage, andTy (such as retrotransposon Ty protein p1). VLPs are discussed furtherin refs. 70-75. Virosomes are discussed further in, for example, ref. 76

E. Bacterial or Microbial Derivatives

Adjuvants suitable for use in the invention include bacterial ormicrobial derivatives such as non-toxic derivatives of enterobacteriallipopolysaccharide (LPS), Lipid A derivatives, immunostimulatoryoligonucleotides and ADP-ribosylating toxins and detoxified derivativesthereof.

Non-toxic derivatives of LPS include monophosphoryl lipid A (MPL) and3-O-deacylated MPL (3dMPL). 3dMPL is a mixture of 3 de-O-acylatedmonophosphoryl lipid A with 4, 5 or 6 acylated chains. A preferred“small particle” form of 3 De-O-acylated monophosphoryl lipid A isdisclosed in ref. 77. Such “small particles” of 3dMPL are small enoughto be sterile filtered through a 0.22 μm membrane[77]. Other non-toxicLPS derivatives include monophosphoryl lipid A mimics, such asaminoalkyl glucosaminide phosphate derivatives e.g. RC-529 [78,79].

Lipid A derivatives include derivatives of lipid A from Escherichia colisuch as OM-174. OM-174 is described for example in refs. 80 & 81.

Immunostimulatory oligonucleotides suitable for use as adjuvants in theinvention include nucleotide sequences containing a CpG motif (adinucleotide sequence containing an unmethylated cytosine linked by aphosphate bond to a guanosine). Double-stranded RNAs andoligonucleotides containing palindromic or poly(dG) sequences have alsobeen shown to be immunostimulatory.

The CpG's can include nucleotide modifications/analogs such asphosphorothioate modifications and can be double-stranded orsingle-stranded. References 82, 83 and 84 disclose possible analogsubstitutions e.g. replacement of guanosine with2′-deoxy-7-deazaguanosine. The adjuvant effect of CpG oligonucleotidesis further discussed in refs. 85-90.

The CpG sequence may be directed to TLR9, such as the motif GTCGTT orTTCGTT [91]. The CpG sequence may be specific for inducing a Thl immuneresponse, such as a CpG-A ODN, or it may be more specific for inducing aB cell response, such a CpG-B ODN. CpG-A and CpG-B ODNs are discussed inrefs. 92-94. Preferably, the CpG is a CpG-A ODN.

Preferably, the CpG oligonucleotide is constructed so that the 5′ end isaccessible for receptor recognition. Optionally, two CpG oligonucleotidesequences may be attached at their 3′ ends to form “immunomers”. See,for example, refs. 91 & 95-97.

Other immunostimulatory oligonucleotides include a double-stranded RNA,or an oligonucleotide containing a palindromic sequence, or anoligonucleotide containing a poly(dG) sequence.

Bacterial ADP-ribosylating toxins and detoxified derivatives thereof maybe used as adjuvants in the invention. Preferably, the protein isderived from E. coli (E. coli heat labile enterotoxin “LT”), cholera(“CT”), or pertussis (“PT”). The use of detoxified ADP-ribosylatingtoxins as mucosal adjuvants is described in ref. 98 and as parenteraladjuvants in ref. 99. The toxin or toxoid is preferably in the form of aholotoxin, comprising both A and B subunits. Preferably, the A subunitcontains a detoxifying mutation; preferably the B subunit is notmutated. Preferably, the adjuvant is a detoxified LT mutant such asLT-K63, LT-R72, and LT-G192. The use of ADP-ribosylating toxins anddetoxified derivaties thereof, particularly LT-K63 and LT-R72, asadjuvants can be found in refs. 100-107. Numerical reference for aminoacid substitutions is preferably based on the alignments of the A and Bsubunits of ADP-ribosylating toxins set forth in ref. 108, specificallyincorporated herein by reference in its entirety.

Compounds of formula I, II or III, or salts thereof, can also be used asadjuvants:

as defined in reference 109, such as ‘ER 803058’, ‘ER 803732’, ‘ER804053’, ER 804058′, ‘ER 804059’, ‘ER 804442’, ‘ER 804680’, ‘ER 804764’,ER 803022 or ‘ER 804057’ e.g.:

F. Human Immunomodulators

Human immunomodulators suitable for use as adjuvants in the inventioninclude cytokines, such as interleukins (e.g. IL-1, IL-2, IL-4, IL-5,IL-6, IL-7, IL-12 [110], etc.) [111], interferons (e.g. interferon-γ),macrophage colony stimulating factor, tumor necrosis factor andmacrophage inflammatory protein-1alpha (MIP-1alpha) and MIP-1beta [112].

G. Bioadhesives and Mucoadhesives

Bioadhesives and mucoadhesives may also be used as adjuvants in theinvention. Suitable bioadhesives include esterified hyaluronic acidmicrospheres [113] or mucoadhesives such as cross-linked derivatives ofpoly(acrylic acid), polyvinyl alcohol, polyvinyl pyrollidone,polysaccharides and carboxymethylcellulose. Chitosan and derivativesthereof may also be used as adjuvants in the invention[114].

H. Microparticles

Microparticles may also be used as adjuvants in the invention.Microparticles (i.e. a particle of ˜100 nm to ˜150 μm in diameter, morepreferably ˜200 nm to ˜30 μm in diameter, and most preferably ˜500 nm to˜10 μm in diameter) formed from materials that are biodegradable andnon-toxic (e.g. a poly(α-hydroxy acid), a polyhydroxybutyric acid, apolyorthoester, a polyanhydride, a polycaprolactone, etc.), withpoly(lactide-co-glycolide) are preferred, optionally treated to have anegatively-charged surface (e.g. with SDS) or a positively-chargedsurface (e.g. with a cationic detergent, such as CTAB).

I. Liposomes (Chapters 13 & 14 of Ref 53)

Examples of liposome formulations suitable for use as adjuvants aredescribed in refs. 115-117.

J. Polyoxyethylene Ether and Polyoxyethylene Ester Formulations

Adjuvants suitable for use in the invention include polyoxyethyleneethers and polyoxyethylene esters [118]. Such formulations furtherinclude polyoxyethylene sorbitan ester surfactants in combination withan octoxynol [119] as well as polyoxyethylene alkyl ethers or estersurfactants in combination with at least one additional non-ionicsurfactant such as an octoxynol [120]. Preferred polyoxyethylene ethersare selected from the following group: polyoxyethylene-9-lauryl ether(laureth 9), polyoxyethylene-9-steoryl ether, polyoxytheylene-8-steorylether, polyoxyethylene-4-lauryl ether, polyoxyethylene-35-lauryl ether,and polyoxyethylene-23-lauryl ether.

K Phosphazenes e.g. PCPP

Phosphazene adjuvants include poly[di(carboxylatophenoxy)phosphazene](“PCPP”) as described, for example, in refs. 121 and 122.

L. Muramyl Peptides

Examples of muramyl peptides suitable for use as adjuvants in theinvention include N-acetyl-muramyl-L-threonyl-D-isoglutamine (thr-MDP),N-acetyl-normuramyl-L-alanyl-D-isoglutamine (nor-MDP), andN-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1′-2′-dipalmitoyl-sn-glycero-3-hydroxyphosphoryloxy)-ethylamineMTP-PE).

M. Imidazoquinoline Compounds.

Imidazoquinoline adjuvants include Imiquimod (“R-837”) [123,124],Resiquimod (“R-848”) [125], and their analogs; and salts thereof (e.g.the hydrochloride salts). Further details about immunostimulatoryimidazoquinolines can be found in references 126 to 130.

N. Thiosemicarbazone Compounds.

Examples of thiosemicarbazone compounds, as well as methods offormulating, manufacturing, and screening for compounds all suitable foruse as adjuvants in the invention include those described in ref. 131.The thiosemicarbazones are particularly effective in the stimulation ofhuman peripheral blood mononuclear cells for the production ofcytokines, such as TNF-α.

O. Tryptanthrin Compounds.

Examples of tryptanthrin compounds, as well as methods of formulating,manufacturing, and screening for compounds all suitable for use asadjuvants in the invention include those described in ref. 132. Thetryptanthrin compounds are particularly effective in the stimulation ofhuman peripheral blood mononuclear cells for the production ofcytokines, such as TNF-α.

P. Nucleoside Analogs

Various nucleoside analogs can be used as adjuvants, such as (a)Isatorabine (ANA-245; 7-thia-8-oxoguanosine):

and prodrugs thereof; (b) ANA975; (c) ANA-025-1; (d) ANA380; (e) thecompounds disclosed in references 133 to 135; (f) a compound having theformula:

-   -   wherein:        -   R₁ and R₂ are each independently H, halo, —NR_(a)R_(b), —OH,            C₁₋₆ alkoxy, substituted C₁₋₆ alkoxy, heterocyclyl,            substituted heterocyclyl, C₆₋₁₀ aryl, substituted C₆₋₁₀            aryl, C₁₋₆ alkyl, or substituted C₁₋₆ alkyl;        -   R₃ is absent, H, C₁₋₆ alkyl, substituted C₁₋₆ alkyl, C₆₋₁₀            aryl, substituted C₆₋₁₀ aryl, heterocyclyl, or substituted            heterocyclyl;        -   R₄ and R₅ are each independently H, halo, heterocyclyl,            substituted heterocyclyl, —C(O)—R_(d), C₁₋₆ alkyl,            substituted C₁₋₆ alkyl, or bound together to form a 5            membered ring as in R₄₋₅:

-   -   -   -   the binding being achieved at the bonds indicated by a

        -   X₁ and X₂ are each independently N, C, O, or S;

        -   R₈ is H, halo, —OH, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,            —OH, —NR_(a)R_(b), —(CH₂)_(n)—O—R_(c), —O—(C₁₋₆ alkyl),            —S(O)_(p)R_(e), or —C(O)—R_(d);

        -   R₉ is H, C₁₋₆ alkyl, substituted C₁₋₆ alkyl, heterocyclyl,            substituted heterocyclyl or R_(9a), wherein R_(9a) is:

-   -   -   -   the binding being achieved at the bond indicated by a

        -   R₁₀ and R₁₁ are each independently H, halo, C₁₋₆ alkoxy,            substituted C₁₋₆ alkoxy, —NR_(a)R_(b), or —OH;

        -   each R_(a) and R_(b) is independently H, C₁₋₆ alkyl,            substituted C₁₋₆ alkyl, —C(O)R_(d), C₆₋₁₀ aryl;

        -   each R_(c) is independently H, phosphate, diphosphate,            triphosphate, C₁₋₆ alkyl, or substituted C₁₋₆ alkyl;

        -   each R_(d) is independently H, halo, C₁₋₆ alkyl, substituted            C₁₋₆ alkyl, C₁₋₆ alkoxy, substituted C₁₋₆ alkoxy, —NH₂,            —NH(C₁₋₆ alkyl), —NH (substituted C₁₋₆ alkyl), —N(C₁₋₆            alkyl)₂, —N(substituted C₁₋₆ alkyl)₂, C₆₋₁₀ aryl, or            heterocyclyl;

        -   each R_(e) is independently H, C₁₋₆ alkyl, substituted C₁₋₆            alkyl, C₆₋₁₀ aryl, substituted C₆₋₁₀ aryl, heterocyclyl, or            substituted heterocyclyl;

        -   each R_(f) is independently H, C₁₋₆ alkyl, substituted C₁₋₆            alkyl, —C(O)R_(d), phosphate, diphosphate, or triphosphate;

        -   each n is independently 0, 1, 2, or 3;

        -   each p is independently 0, 1, or 2; or

    -   or (g) a pharmaceutically acceptable salt of any of (a) to (f),        a tautomer of any of (a) to (f), or a pharmaceutically        acceptable salt of the tautomer.

Q. Lipids Linked to a Phosphate-Containing Acyclic Backbone

Adjuvants containing lipids linked to a phosphate-containing acyclicbackbone include the TLR4 antagonist E5564 [136,137]:

R. Small Molecule Immunopotentiators (SMIPs)

SMIPs include:

-   N2-methyl-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2,N2-dimethyl-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-ethyl-N2-methyl-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-methyl-1-(2-methylpropyl)-N2-propyl-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   1-(2-methylpropyl)-N2-propyl-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-butyl-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-butyl-N2-methyl-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-methyl-1-(2-methylpropyl)-N2-pentyl-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-methyl-1-(2-methylpropyl)-N2-prop-2-enyl-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   1-(2-methylpropyl)-2-[(phenylmethyl)thio]-1H-imidazo[4,5-c]quinolin-4-amine;-   1-(2-methylpropyl)-2-(propylthio)-1H-imidazo[4,5-c]quinolin-4-amine;-   2-[[4-amino-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl](methyl)amino]ethanol;-   2-[[4-amino-1-(2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl](methyl)amino]ethyl    acetate;-   4-amino-1-(2-methylpropyl)-1,3-dihydro-2H-imidazo[4,5-c]quinolin-2-one;-   N2-butyl-1-(2-methylpropyl)-N4,N4-bis(phenylmethyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-butyl-N2-methyl-1-(2-methylpropyl)-N4,N4-bis(phenylmethyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2-methyl-1-(2-methylpropyl)-N4,N4-bis(phenylmethyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   N2,N2-dimethyl-1-(2-methylpropyl)-N4,N4-bis(phenylmethyl)-1H-imidazo[4,5-c]quinoline-2,4-diamine;-   1-{4-amino-2-[methyl(propyl)amino]-1H-imidazo[4,5-c]quinolin-1-yl}-2-methylpropan-2-ol;-   1-[4-amino-2-(propylamino)-1H-imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol;-   N4,N4-dibenzyl-1-(2-methoxy-2-methylpropyl)-N2-propyl-1H-imidazo[4,5-c]quinoline-2,4-diamine.

S. Proteosomes

One adjuvant is an outer membrane protein proteosome preparationprepared from a first Gram-negative bacterium in combination with aliposaccharide preparation derived from a second Gram-negativebacterium, wherein the outer membrane protein proteosome andliposaccharide preparations form a stable non-covalent adjuvant complex.Such complexes include “IVX-908”, a complex comprised of Neisseriameningitidis outer membrane and lipopolysaccharides. They have been usedas adjuvants for influenza vaccines [138].

T. Other Adjuvants

Other substances that act as immunostimulating agents are disclosed inchapter 7 of ref. 53. references 53 and 58. Further useful adjuvantsubstances include:

-   -   Methyl inosine 5′-monophosphate (“MIMP”) [139].    -   A polyhydroxlated pyrrolizidine compound [140], such as one        having formula:

-   -   where R is selected from the group comprising hydrogen, straight        or branched, unsubstituted or substituted, saturated or        unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and        aryl groups, or a pharmaceutically acceptable salt or derivative        thereof. Examples include, but are not limited to: casuarine,        casuarine-6-α-D-glucopyranose, 3-epi-casuarine, 7-epi-casuarine,        3,7-diepi-casuarine, etc.    -   A gamma inulin [141] or derivative thereof, such as algammulin.    -   Compounds disclosed in reference 142.    -   Compounds disclosed in reference 143, including: Acylpiperazine        compounds, Indoledione compounds, Tetrahydraisoquinoline (THIQ)        compounds, Benzocyclodione compounds, Aminoazavinyl compounds,        Aminobenzimidazole quinolinone (ABIQ) compounds [144,145],        Hydrapthalamide compounds, Benzophenone compounds, Isoxazole        compounds, Sterol compounds, Quinazilinone compounds, Pyrrole        compounds [146], Anthraquinone compounds, Quinoxaline compounds,        Triazine compounds, Pyrazalopyrimidine compounds, and Benzazole        compounds [147].    -   Loxoribine (7-allyl-8-oxoguanosine) [148].    -   A formulation of a cationic lipid and a (usually neutral)        co-lipid, such as        aminopropyl-dimethyl-myristoleyloxy-propanaminium        bromide-diphytanoylphosphatidyl-ethanolamine (“Vaxfectin™”) or        aminopropyl-dimethyl-bis-dodecyloxy-propanaminium        bromide-dioleoylphosphatidyl-ethanolamine (“GAP-DLRIE:DOPE”).        Formulations containing        (±)-N-(3-aminopropyl)-N,N-dimethyl-2,3-bis(syn-9-tetradeceneyloxy)-1-propanaminium        salts are preferred [149].

The invention may also comprise combinations of one or more of theadjuvants identified above. For example, the following combinations maybe used as adjuvant compositions in the invention: (1) a saponin and anoil-in-water emulsion [150]; (2) a saponin (e.g. QS21)+a non-toxic LPSderivative (e.g. 3dMPL) [151]; (3) a saponin (e.g. QS21)+a non-toxic LPSderivative (e.g. 3dMPL)+a cholesterol; (4) a saponin (e.g.QS21)+3dMPL+IL-12 (optionally+a sterol) [152]; (5) combinations of 3dMPLwith, for example, QS21 and/or oil-in-water emulsions [153]; (6) SAF,containing 10% squalane, 0.4% Tween 80™, 5% pluronic-block polymer L121,and thr-MDP, either microfluidized into a submicron emulsion or vortexedto generate a larger particle size emulsion. (7) Ribi™ adjuvant system(RAS), (Ribi Immunochem) containing 2% squalene, 0.2% Tween 80, and oneor more bacterial cell wall components from the group consisting ofmonophosphorylipid A (MPL), trehalose dimycolate (TDM), and cell wallskeleton (CWS), preferably MPL+CWS (Detox™); (8) one or more mineralsalts (such as an aluminum salt)+a non-toxic derivative of LPS (such as3dMPL); and (9) one or more mineral salts (such as an aluminum salt)+animmunostimulatory oligonucleotide (such as a nucleotide sequenceincluding a CpG motif).

The compositions of the invention will preferably elicit both a cellmediated immune response as well as a humoral immune response in orderto effectively address a uropathogenic infection. This immune responsewill preferably induce long lasting (e.g. neutralising) antibodies and acell mediated immunity that can quickly respond upon exposure toMNEC-associated antigens.

Two types of T cells, CD4 and CD8 cells, are generally thought necessaryto initiate and/or enhance cell mediated immunity and humoral immunity.CD8 T cells can express a CD8 co-receptor and are commonly referred toas cytotoxic T lymphocytes (CTLs). CD8 T cells are able to recognized orinteract with antigens displayed on MHC Class I molecules. CD4 T cellscan express a CD4 co-receptor and are commonly referred to as T helpercells. CD4 T cells are able to recognize antigenic peptides bound to MHCclass II molecules. Upon interaction with a MHC class II molecule, theCD4 cells can secrete factors such as cytokines. These secretedcytokines can activate B cells, cytotoxic T cells, macrophages, andother cells that participate in an immune response. Helper T cells orCD4⁺ cells can be further divided into two functionally distinctsubsets: TH1 phenotype and TH2 phenotypes which differ in their cytokineand effector function.

Activated TH1 cells enhance cellular immunity (including an increase inantigen-specific CTL production) and are therefore of particular valuein responding to intracellular infections. Activated TH1 cells maysecrete one or more of IL-2, IFN-γ, and TNF-β. A TH1 immune response mayresult in local inflammatory reactions by activating macrophages, NK(natural killer) cells, and CD8 cytotoxic T cells (CTLs). A TH1 immuneresponse may also act to expand the immune response by stimulatinggrowth of B and T cells with IL-12. TH1 stimulated B cells may secreteIgG2a.

Activated TH2 cells enhance antibody production and are therefore ofparticular value in responding to extracellular infections. ActivatedTH2 cells may secrete one or more of IL-4, IL-5, IL-6, and IL-10. A TH2immune response may result in the production of IgG1, IgE, IgA andmemory B cells for future protection.

An enhanced immune response may include one or more of an enhanced TH1immune response and a TH2 immune response. An enhanced TH1 immuneresponse may include one or more of an increase in CTLs, an increase inone or more of the cytokines associated with a TH1 immune response (suchas IL-2, IFN-γ, and TNF-β), an increase in activated macrophages, anincrease in NK activity, or an increase in the production of IgG2a.Preferably, the enhanced TH1 immune response will include an increase inIgG2a production. An enhanced TH2 immune response may include one ormore of an increase in one or more of the cytokines associated with aTH2 immune response (such as IL-4, IL-5, IL-6 and IL-10), or an increasein the production of IgG1, IgE, IgA and memory B cells. Preferably, theenhanced TH2 immune response will include an increase in IgG1production.

A TH1 immune response may be elicited using a TH1 adjuvant. A TH1adjuvant will generally elicit increased levels of IgG2a productionrelative to immunization of the antigen without adjuvant. TH1 adjuvantssuitable for use in the invention may include for example saponinformulations, virosomes and virus like particles, non-toxic derivativesof enterobacterial lipopolysaccharide (LPS), immunostimulatoryoligonucleotides Immunostimulatory oligonucleotides, such asoligonucleotides containing a CpG motif, are preferred TH1 adjuvants foruse in the invention.

A TH2 immune response may be elicited using a TH2 adjuvant. A TH2adjuvant will generally elicit increased levels of IgG1 productionrelative to immunization of the antigen without adjuvant. TH2 adjuvantssuitable for use in the invention include, for example, mineralcontaining compositions, oil-emulsions, and ADP-ribosylating toxins anddetoxified derivatives hereof. Mineral containing compositions, such asaluminium salts are preferred TH2 adjuvants for use in the invention.

Preferably, the invention includes a composition comprising acombination of a TH1 adjuvant and a TH2 adjuvant. Preferably, such acomposition elicits an enhanced TH1 and an enhanced TH2 response i.e. anincrease in the production of both IgG1 and IgG2a production relative toimmunization without an adjuvant. Still more preferably, the compositioncomprising a combination of a TH1 and a TH2 adjuvant elicits anincreased TH1 and/or an increased TH2 immune response relative toimmunization with a single adjuvant (i.e. relative to immunization witha TH1 adjuvant alone or immunization with a TH2 adjuvant alone).

The immune response may be one or both of a TH1 immune response and aTH2 response. Preferably, immune response provides for one or both of anenhanced TH1 response and an enhanced TH2 response.

The enhanced immune response may be one or both of a systemic and amucosal immune response. Preferably, the immune response provides forone or both of an enhanced systemic and an enhanced mucosal immuneresponse. Preferably the mucosal immune response is a TH2 immuneresponse. Preferably, the mucosal immune response includes an increasein the production of IgA.

The use of an aluminium hydroxide or aluminium phosphate adjuvant isparticularly preferred, and antigens are generally adsorbed to thesesalts. Calcium phosphate is another preferred adjuvant.

The pH of compositions of the invention is preferably between 6 and 8,preferably about 7. Stable pH may be maintained by the use of a buffer.Where a composition comprises an aluminium hydroxide salt, it ispreferred to use a histidine buffer [154]. The composition may besterile and/or pyrogen-free. Compositions of the invention may beisotonic with respect to humans.

Compositions may be presented in vials, or they may be presented inready-filled syringes. The syringes may be supplied with or withoutneedles. A syringe will include a single dose of the composition,whereas a vial may include a single dose or multiple doses. Injectablecompositions will usually be liquid solutions or suspensions.Alternatively, they may be presented in solid form (e.g. freeze-dried)for solution or suspension in liquid vehicles prior to injection.

Compositions of the invention may be packaged in unit dose form or inmultiple dose form. For multiple dose forms, vials are preferred topre-filled syringes. Effective dosage volumes can be routinelyestablished, but a typical human dose of the composition for injectionhas a volume of 0.5 ml.

Where a composition of the invention is to be prepared extemporaneouslyprior to use (e.g. where a component is presented in lyophilised form)and is presented as a kit, the kit may comprise two vials, or it maycomprise one ready-filled syringe and one vial, with the contents of thesyringe being used to reactivate the contents of the vial prior toinjection.

Thus the invention provides for a kit comprising a first component and asecond component, wherein: the first component comprises one or morepolypeptide, antibody, vesicle and/or nucleic acid of the invention; andthe second component comprises one or more of the following:instructions for administering a composition to a patient, a syringe orother delivery device, an adjuvant, and/or a pharmaceutically acceptableformulating solution.

The invention also provides a delivery device (e.g. a syringe)pre-filled with the immunogenic compositions of the invention.

Immunogenic compositions used as vaccines comprise an immunologicallyeffective amount of antigen(s), as well as any other components, asneeded. By ‘immunologically effective amount’, it is meant that theadministration of that amount to an individual, either in a single doseor as part of a series, is effective for treatment or prevention. Thisamount varies depending upon the health and physical condition of theindividual to be treated, age, the taxonomic group of individual to betreated (e.g. non-human primate, primate, etc.), the capacity of theindividual's immune system to synthesise antibodies, the degree ofprotection desired, the formulation of the vaccine, the treatingdoctor's assessment of the medical situation, and other relevantfactors. It is expected that the amount will fall in a relatively broadrange that can be determined through routine trials, and a typicalquantity of each meningococcal saccharide antigen per dose is between 1μg and 10 mg per antigen.

Pharmaceutical Uses

The invention also provides a method of treating a patient, comprisingadministering to the patient a therapeutically effective amount of acomposition of the invention. The patient may either be at risk from thedisease themselves or may be a pregnant woman (‘maternal immunisation’[155]).

The invention provides nucleic acid, polypeptide, vesicle or antibody ofthe invention for use as medicaments (e.g. as immunogenic compositionsor as vaccines) or as diagnostic reagents. It also provides the use ofnucleic acid, polypeptide, vesicle or antibody of the invention in themanufacture of: (i) a medicament for treating or preventing diseaseand/or infection caused by an ExPEC bacterium; (ii) a diagnostic reagentfor detecting the presence of or of antibodies raised against an ExPECbacterium; and/or (iii) a reagent which can raise antibodies against anExPEC bacterium. Said ExPEC bacterium can be of any serotype or strain,but are preferably MNEC bacteria e.g. K1 serotype and/or B2 MLEE type.

The invention is useful for the prevention and/or treatment of diseasessuch as bacteremia, meningitis, a urinary tract infection,pyelonephritis and/or cystitis. The invention is particularly useful forthe treatment of sepsis and/or meningitis

The patient is preferably a human. The human may be a child (e.g. agedbetween 0 and 18 years, or between 0-5 years), may be an adolescent(e.g. aged 15-19), an adult (e.g. aged 19-54) or may be elderly (e.g. 55years or older). Female adolescents and adults are a preferred group ofpatients. A vaccine intended for children or adolescents may also beadministered to adults e.g. to assess safety, dosage, immunogenicity,etc.

Other possible patient animals include dogs, which may be carriers ofExPEC [156,157].

One way of checking efficacy of therapeutic treatment involvesmonitoring infection after administration of the composition of theinvention. One way of checking efficacy of prophylactic treatmentinvolves monitoring immune responses against an administered polypeptideafter administration Immunogenicity of compositions of the invention canbe determined by administering them to test subjects (e.g. children12-16 months age, or animal models e.g. a murine model) and thendetermining standard parameters including ELISA titres (GMT) of IgG.These immune responses will generally be determined around 4 weeks afteradministration of the composition, and compared to values determinedbefore administration of the composition. Where more than one dose ofthe composition is administered, more than one post-administrationdetermination may be made. Efficacy may also be assessed using adultmice models of sepsis, mouse models of UTI, and passive protection frommeningitis in infant rats.

Administration of polypeptide antigens is a preferred method oftreatment for inducing immunity. Administration of antibodies of theinvention is another preferred method of treatment. This method ofpassive immunisation is particularly useful for newborn children or forpregnant women. This method will typically use monoclonal antibodies,which will be humanised or fully human

Compositions of the invention will generally be administered directly toa patient. Direct delivery may be accomplished by parenteral injection(e.g. subcutaneously, intraperitoneally, intravenously, intramuscularly,or to the interstitial space of a tissue), or by rectal, oral (e.g.tablet, spray), vaginal, topical, transdermal, transcutaneous,intranasal, sublingual, ocular, aural, pulmonary or other mucosaladministration. Intramuscular administration to the thigh or the upperarm is preferred. Injection may be via a needle (e.g. a hypodermicneedle), but needle-free injection may alternatively be used. A typicalintramuscular dose is 0.5 ml.

The invention may be used to elicit systemic and/or mucosal immunity.Preferably the enhanced systemic and/or mucosal immunity is reflected inan enhanced TH1 and/or TH2 immune response. Preferably, the enhancedimmune response includes an increase in the production of IgG1 and/orIgG2a and/or IgA.

Dosage treatment can be a single dose schedule or a multiple doseschedule. Multiple doses may be used in a primary immunisation scheduleand/or in a booster immunisation schedule. A primary dose schedule maybe followed by a booster dose schedule. In a multiple dose schedule thevarious doses may be given by the same or different routes e.g. aparenteral prime and mucosal boost, a mucosal prime and parenteralboost, etc. Suitable timing between priming doses (e.g. between 4-16weeks), and between priming and boosting, can be routinely determined.For example, a primary course of vaccination may include 1-10 separatedoses, followed by other doses given at subsequent time intervalsrequired to maintain and/or reinforce an immune response, for example,at 1-4 months for a second dose, and if needed, a subsequent dose ordoses after several months.

Bacterial infections affect various areas of the body and socompositions may be prepared in various forms. For example, thecompositions may be prepared as injectables, either as liquid solutionsor suspensions. Solid forms suitable for solution in, or suspension in,liquid vehicles prior to injection can also be prepared (e.g. alyophilised or a spray-freeze dried composition). The composition may beprepared for topical administration e.g. as an ointment, cream orpowder. The composition be prepared for oral administration e.g. as atablet or capsule, as a spray, or as a syrup (optionally flavoured). Thecomposition may be prepared for pulmonary administration e.g. as aninhaler, using a fine powder or a spray. The composition may be preparedas a suppository or pessary. The composition may be prepared for nasal,aural or ocular administration e.g. as spray, drops, gel or powder [e.g.refs 158 & 159]. The composition may be in kit form, designed such thata combined composition is reconstituted just prior to administration toa patient. Such kits may comprise one or more antigens in liquid formand one or more lyophilised antigens.

Compositions of the invention may be administered to patients atsubstantially the same time as (e.g. during the same medicalconsultation or visit to a healthcare professional) other vaccines e.g.at substantially the same time as a measles vaccine, a mumps vaccine, arubella vaccine, a MMR vaccine, a varicella vaccine, a MMRV vaccine, adiphtheria vaccine, a tetanus vaccine, a pertussis vaccine, a DTPvaccine, a conjugated H. influenzae type b vaccine, a humanpapillomavirus vaccine, an inactivated poliovirus vaccine, a hepatitis Bvirus vaccine, a pneumococcal conjugate vaccine, a meningococcalconjugate vaccine, etc. Similarly they may be administered to patientsat substantially the same time as (e.g. during the same medicalconsultation or visit to a healthcare professional) an antibiotic, andin particular an antibiotic compound active against MNEC.

Further Antigenic Components of Compositions of the Invention

The invention also provides a composition comprising a polypeptide orthe invention and one or more of the following further antigens:

-   -   a saccharide antigen from N. meningitidis serogroup A, C, W135        and/or Y (preferably all four), such as the oligosaccharide        disclosed in ref. 160 from serogroup C [see also ref. 161] or        the oligosaccharides of ref. 162.    -   an antigen from N. meningitidis serogroup B such as those        disclosed in refs. 163-171, etc.    -   a saccharide antigen from Streptococcus pneumoniae [e.g. 172,        173, 174].    -   an antigen from hepatitis A virus, such as inactivated virus        [e.g. 175, 176].    -   an antigen from hepatitis B virus, such as the surface and/or        core antigens [e.g. 176, 177].    -   a diphtheria antigen, such as a diphtheria toxoid [e.g. chapter        3 of ref. 178] e.g. the CRM₁₉₇ mutant [e.g. 179].    -   an antigen from hepatitis C virus [e.g. 180].    -   an antigen from HIV [181]    -   a tetanus antigen, such as a tetanus toxoid [e.g. chapter 4 of        ref. 178].    -   an antigen from Bordetella pertussis, such as pertussis        holotoxin (PT) and filamentous haemagglutinin (FHA) from B.        pertussis, optionally also in combination with pertactin and/or        agglutinogens 2 and 3 [e.g. refs. 182 & 183].    -   a saccharide antigen from Haemophilus influenzae B [e.g. 161].    -   polio antigen(s) [e.g. 184, 185] such as IPV.    -   measles, mumps and/or rubella antigens [e.g. chapters 9, 10 & 11        of ref. 178].    -   varicella antigens.    -   influenza antigen(s) [e.g. chapter 19 of ref. 178], such as the        haemagglutinin and/or neuraminidase surface proteins. Influenza        antigens may be derived from interpandemic (annual) flu strains.        Influenza antigens may be derived from strains with the        potential to cause a pandemic outbreak (i.e., influenza strains        with new haemagglutinin compared to the haemagglutinin in        currently circulating strains, or influenza strains which are        pathogenic in avian subjects and have the potential to be        transmitted horizontally in the human population, or influenza        strains which are pathogenic to humans). Influenza antigens may        be derived from viruses grown in eggs or cell culture.    -   an antigen from Moraxella catarrhalis [e.g. 186].    -   a saccharide antigen from Streptococcus agalactiae (group B        streptococcus).    -   an protein antigen from Streptococcus agalactiae (group B        streptococcus) [e.g. 187, 188].    -   an antigen from N. gonorrhoeae [e.g. 189-192].    -   an antigen from Chlamydia pneumoniae [e.g. refs. 193 to 199] or        a combination of antigens from C. pneumoniae [e.g. 200].    -   an antigen from Chlamydia trachomatis, or a combination of        antigens from Cdrachomatis [e.g. 201].    -   an antigen from Porphyromonas gingivalis [e.g. 202].    -   rabies antigen(s) [e.g. 203] such as lyophilised inactivated        virus [e.g. 204, RabAvert™]    -   antigen(s) from a paramyxovirus such as respiratory syncytial        virus (RSV [205, 206]) and/or parainfluenza virus (PIV3 [207]).    -   an antigen from Bacillus anthracis [e.g. 208, 209, 210].    -   an antigen from Streptococcus pyogenes (group A streptococcus)        [e.g. 188,211, 212].    -   an antigen from Staphylococcus aureus [e.g. 213].    -   an antigen from a virus in the flaviviridae family (genus        flavivirus), such as from yellow fever virus, Japanese        encephalitis virus, four serotypes of Dengue viruses, tick-borne        encephalitis virus, West Nile virus.    -   a pestivirus antigen, such as from classical porcine fever        virus, bovine viral diarrhoea virus, and/or border disease        virus.    -   a parvovirus antigen e.g. from parvovirus B19.    -   a human papilloma virus (HPV) antigen[214]

The composition may comprise one or more of these further antigens.

Preferred Gonococcal antigens include one or more of ngs13 (OmpA), OmpH,ngs576 (peptidyl-prolyl cis/trans isomerase (PPIase) protein), ngs41 andngs117.

Preferred HPV antigens include one or more of HPV 16, HPV 18, HPV 6 andHPV 11.

Preferred Chlamydia trachomatis antigens include one or more of: CT045,CT089, CT242, CT316, CT381. CT396, CT398, CT444, CT467, CT547, CT587,CT823, CT761 and specific combinations of these antigens as disclosed inWO 05/002619.

Preferred Chlamydia pneumoniae antigens include one or more of: CPn0324,Cpn0301, Cpn0482, Cpn0503, Cpn0525, Cpn0558, Cpn0584, Cpn0800, Cpn0979,Cpn0498, Cpn0300, Cpn0042, Cpn0013, Cpn450, Cpn0661, Cpn0557, Cpn0904,Clpn0795, Cpn0186 and Cpn0604 and specific combinations of theseantigens as disclosed in WO 05/084306.

Preferred GBS antigens include one or more of GBS80, GBS 104, GBS 59,GBS 67, GBS 322 and GBS 276.

In another embodiment, antigens of the invention are combined with oneor more additional, non-E. coli antigens suitable for use in a vaccinedesigned to protect females against genitourinary and/or sexuallytransmitted diseases. For example, the antigens may be combined with anantigen derived from the group consisting of Streptococcus agalactiae,Chlamydia trachomatis, Neisseria gonorrhoeae, papillomavirus and herpessimplex virus. Where human papillomavirus antigens are used, they may befrom one or more of HPV 16, HPV 18, HPV 6 and/or HPV 11.

In another embodiment, the antigen combinations of the invention arecombined with one or more additional, non-ExPEC antigens suitable foruse in a vaccine designed to protect elderly or immunocompromisedindividuals. For example, the antigen combinations may be combined withan antigen derived from the group consisting of Enterococcus faecalis,Staphylococcus aureus, Staphylococcus epidermis, Pseudomonas aeruginosa,Legionella pneumophila, Listeria monocytogenes, Neisseria meningitidies,influenza, and parainfluenza virus (TN′).

Toxic protein antigens may be detoxified where necessary (e.g.detoxification of pertussis toxin by chemical and/or genetic means[183]).

Where a diphtheria antigen is included in the composition it ispreferred also to include tetanus antigen and pertussis antigens.Similarly, where a tetanus antigen is included it is preferred also toinclude diphtheria and pertussis antigens. Similarly, where a pertussisantigen is included it is preferred also to include diphtheria andtetanus antigens. DTP combinations are thus preferred.

Saccharide antigens are preferably in the form of conjugates. Carrierproteins for the conjugates include bacterial toxins (such as diphtheriatoxoid or tetanus toxoid), the N. meningitidis outer membrane protein[215], synthetic peptides [216,217], heat shock proteins [218,219],pertussis proteins [220,221], protein D from H. influenzae [222,223],cytokines [224], lymphokines [224], H. influenzae proteins, hormones[224], growth factors [224], toxin A or B from C. difficile [225],iron-uptake proteins [226], artificial proteins comprising multiplehuman CD4+ T cell epitopes from various pathogen-derived antigens [227]such as the N19 protein [228], pneumococcal surface protein PspA [229],pneumolysis [230], etc. A preferred carrier protein is CRM197 protein[231].

Antigens in the composition will typically be present at a concentrationof at least 1 μg/ml each. In general, the concentration of any givenantigen will be sufficient to elicit an immune response against thatantigen.

Antigens are preferably adsorbed to an aluminium salt.

Nucleic Acid Immunisation

The immunogenic compositions described above include polypeptideantigens from MNEC. As an alternative to using proteins antigens in theimmunogenic compositions of the invention, nucleic acid (preferably DNAe.g. in the form of a plasmid) encoding the antigen may be used, to givecompositions, methods and uses based on nucleic acid immunisation.Nucleic acid immunisation is now a developed field (e.g. see references232 to 239 etc.), and has been applied to many vaccines.

The nucleic acid encoding the immunogen is expressed in vivo afterdelivery to a patient and the expressed immunogen then stimulates theimmune system. The active ingredient will typically take the form of anucleic acid vector comprising: (i) a promoter; (ii) a sequence encodingthe immunogen, operably linked to the promoter; and optionally (iii) aselectable marker. Preferred vectors may further comprise (iv) an originof replication; and (v) a transcription terminator downstream of andoperably linked to (ii). In general, (i) & (v) will be eukaryotic and(iii) & (iv) will be prokaryotic.

Preferred promoters are viral promoters e.g. from cytomegalovirus (CMV).The vector may also include transcriptional regulatory sequences (e.g.enhancers) in addition to the promoter and which interact functionallywith the promoter. Preferred vectors include the immediate-early CMVenhancer/promoter, and more preferred vectors also include CMV intron A.The promoter is operably linked to a downstream sequence encoding animmunogen, such that expression of the immunogen-encoding sequence isunder the promoter's control.

Where a marker is used, it preferably functions in a microbial host(e.g. in a prokaryote, in a bacteria, in a yeast). The marker ispreferably a prokaryotic selectable marker (e.g. transcribed under thecontrol of a prokaryotic promoter). For convenience, typical markers areantibiotic resistance genes.

The vector of the invention is preferably an autonomously replicatingepisomal or extrachromosomal vector, such as a plasmid.

The vector of the invention preferably comprises an origin ofreplication. It is preferred that the origin of replication is active inprokaryotes but not in eukaryotes.

Preferred vectors thus include a prokaryotic marker for selection of thevector, a prokaryotic origin of replication, but a eukaryotic promoterfor driving transcription of the immunogen-encoding sequence. Thevectors will therefore (a) be amplified and selected in prokaryotichosts without polypeptide expression, but (b) be expressed in eukaryotichosts without being amplified. This arrangement is ideal for nucleicacid immunization vectors.

The vector of the invention may comprise a eukaryotic transcriptionalterminator sequence downstream of the coding sequence. This can enhancetranscription levels. Where the coding sequence does not have its own,the vector of the invention preferably comprises a polyadenylationsequence. A preferred polyadenylation sequence is from bovine growthhormone.

The vector of the invention may comprise a multiple cloning site.

In addition to sequences encoding the immunogen and a marker, the vectormay comprise a second eukaryotic coding sequence. The vector may alsocomprise an IRES upstream of said second sequence in order to permittranslation of a second eukaryotic polypeptide from the same transcriptas the immunogen. Alternatively, the immunogen-coding sequence may bedownstream of an IRES.

The vector of the invention may comprise unmethylated CpG motifs e.g.unmethylated DNA sequences which have in common a cytosine preceding aguanosine, flanked by two 5′ purines and two 3′ pyrimidines. In theirunmethylated form these DNA motifs have been demonstrated to be potentstimulators of several types of immune cell.

Vectors may be delivered in a targeted way. Receptor-mediated DNAtherapy techniques are described in, for example, references 240 to 245.Therapeutic compositions containing a nucleic acid are administered in arange of about 100 ng to about 200 mg of DNA for local administration ina gene therapy protocol. Concentration ranges of about 500 ng to about50 mg, about 1 μg to about 2 mg, about 5 μg to about 500 μg, and about20 μg to about 100 μg of DNA can also be used during a gene therapyprotocol. Factors such as method of action (e.g. for enhancing orinhibiting levels of the encoded gene product) and efficacy oftransformation and expression are considerations which will affect thedosage required for ultimate efficacy. Where greater expression isdesired over a larger area of tissue, larger amounts of vector or thesame amounts re-administered in a successive protocol ofadministrations, or several administrations to different adjacent orclose tissue portions may be required to effect a positive therapeuticoutcome. In all cases, routine experimentation in clinical trials willdetermine specific ranges for optimal therapeutic effect.

Vectors can be delivered using gene delivery vehicles. The gene deliveryvehicle can be of viral or non-viral origin (see generally references246 to 249).

Viral-based vectors for delivery of a desired nucleic acid andexpression in a desired cell are well known in the art. Exemplaryviral-based vehicles include, but are not limited to, recombinantretroviruses (e.g. references 250 to 260), alphavirus-based vectors(e.g. Sindbis virus vectors, Semliki forest virus (ATCC VR-67; ATCCVR-1247), Ross River virus (ATCC VR-373; ATCC VR-1246) and Venezuelanequine encephalitis virus (ATCC VR-923; ATCC VR-1250; ATCC VR 1249; ATCCVR-532); hybrids or chimeras of these viruses may also be used),poxvirus vectors (e.g. vaccinia, fowlpox, canarypox, modified vacciniaAnkara, etc.), adenovirus vectors, and adeno-associated virus (AAV)vectors (e.g. see refs. 261 to 266). Administration of DNA linked tokilled adenovirus [267] can also be employed.

Non-viral delivery vehicles and methods can also be employed, including,but not limited to, polycationic condensed DNA linked or unlinked tokilled adenovirus alone [e.g. 267], ligand-linked DNA [268], eukaryoticcell delivery vehicles cells [e.g. refs. 269 to 273] and nucleic chargeneutralization or fusion with cell membranes. Naked DNA can also beemployed. Exemplary naked DNA introduction methods are described inrefs. 274 and 275. Liposomes (e.g. immunoliposomes) that can act as genedelivery vehicles are described in refs. 276 to 280. Additionalapproaches are described in references 281 & 282.

Further non-viral delivery suitable for use includes mechanical deliverysystems such as the approach described in ref. 282. Moreover, the codingsequence and the product of expression of such can be delivered throughdeposition of photopolymerized hydrogel materials or use of ionizingradiation [e.g. refs. 283 & 284]. Other conventional methods for genedelivery that can be used for delivery of the coding sequence include,for example, use of hand-held gene transfer particle gun or use ofionizing radiation for activating transferred genes [283 & 286].

Delivery DNA using PLG {poly(lactide-co-glycolide)} microparticles is aparticularly preferred method e.g. by adsorption to the microparticles,which are optionally treated to have a negatively-charged surface (e.g.treated with SDS) or a positively-charged surface (e.g. treated with acationic detergent, such as CTAB).

General

The term “comprising” encompasses “including” as well as “consisting”e.g. a composition “comprising” X may consist exclusively of X or mayinclude something additional e.g. X+Y.

The term “about” in relation to a numerical value x means, for example,x±10%.

The word “substantially” does not exclude “completely” e.g. acomposition which is “substantially free” from Y may be completely freefrom Y. Where necessary, the word “substantially” may be omitted fromthe definition of the invention.

The N-terminus residues in the amino acid sequences in the sequencelisting are generally given as the amino acid encoded by the first codonin the corresponding nucleotide sequence. Where the first codon is notATG (e.g. SEQ ID NOs: 1, 7, 19, 39, 43, 49, 51, 55, 63, 73, 119, 127,129, 137, 143, 145, 147, 149, 155, 159, 165, 173, 181, 183, 205, 209,219, 221, 229, 235, 247, 263, 269, 271, 297, 303, 325, 327, 341, 345,359, 363, 373, 395, 401, 413, 443, 447, 453, 459, 463, 465, 469, 473,477, 479, 495, 505, 507, 517, 527, 533, 535, 537, 539, 549, 569, 575,579, 581, 583, 587, 591, 593, 599, 613, 621, 635, 637, 643, 655, 661,665, 669, 671, 681, 683, 687, 693, 707, 709, 721, 723, 729, 743, 757,763, 765, 769, 781, 791, 793, 813, 817, 823, 837, 839, 855, 861, 865,881, 885, 897, 907, 909, 911, 917, 921, 941, 949, 957, 965, 975, 977,979, 981, 983, 989, 993, 997, 1001, 1005, 1011, 1015, 1017, 1019, 1027,1029, 1033, 1037, 1043, 1051, 1053, 1073, 1087, 1097, 1101, 1103, 1107,1113, 1115, 1117, 1121, 1123, 1135, 1143, 1147, 1149, 1175, 1181, 1227,1263, 1271, 1287, 1295, 1305, 1307, 1311, 1317, 1323, 1331, 1341, 1345,1349, 1351, 1355, 1359, 1363, 1373, 1375, 1383, 1399, 1411, 1415, 1417,1423, 1433, 1451, 1477, 1483, 1485, 1493, 1495, 1501, 1503, 1511, 1519,1521, 1525, 1529, 1553, 1559, 1579, 1585, 1603, 1609, 1611, 1619, 1621,1623, 1629, 1631, 1643, 1653, 1669, 1675, 1677, 1687, 1693, 1705, 1713,1717, 1727, 1739, 1751, 1763, 1769, 1785, 1789, 1799, 1801, 1817, 1819,1825, 1827, 1829, 1831, 1833, 1839, 1857, 1867, 1911, 1919, 1925, 1929,1939, 1947, 1955, 1957, 1979, 1981, 1985, 1989, 1993, 1995, 2003, 2005,2009, 2027, 2035, 2037, 2047, 2049, 2059, 2063, 2079, 2093, 2123, 2135,2139, 2141, 2163, 2165, 2173, 2177, 2189, 2195, 2197, 2203, 2209, 2211,2241, 2247, 2275, 2291, 2321, 2327, 2357, 2361, 2373, 2377, 2379, 2397,2399, 2405, 2433, 2437, 2443, 2447, 2451, 2455, 2457, 2487, 2491, 2503,2505, 2507, 2513, 2515, 2521, 2541, 2545, 2549, 2553, 2565, 2577, 2579,2591, 2613, 2623, 2629, 2631, 2633, 2635, 2639, 2641, 2649, 2657, 2659,2661, 2663, 2665, 2667, 2669, 2671, 2681, 2687, 2689, 2699, 2701, 2705,2713, 2719, 2721, 2729, 2735, 2743, 2745, 2759, 2781, 2785, 2787, 2789,2795, 2801, 2809, 2813, 2815, 2823, 2825, 2845, 2847, 2869, 2885, 2891,2897, 2905, 2907, 2911, 2913, 2915, 2925, 2939, 2945, 2947, 2975, 2993,3015, 3043, 3055, 3061, 3063, 3075, 3107, 3117, 3127, 3131, 3175, 3179,3191, 3193, 3207, 3209, 3213, 3221, 3229, 3243, 3253, 3259, 3261, 3267,3283, 3289, 3307, 3313, 3331, 3337, 3339, 3353, 3357, 3361, 3367, 3379,3381, 3383, 3411, 3419, 3429, 3433, 3435, 3437, 3445, 3449, 3453, 3471,3491, 3501, 3507, 3515, 3559, 3563, 3571, 3573, 3577, 3579, 3583, 3595,3607, 3619, 3621, 3623, 3625, 3629, 3633, 3635, 3637, 3663, 3671, 3679,3685, 3687, 3699, 3701, 3707, 3713, 3721, 3723, 3739, 3741, 3743, 3759,3763, 3777, 3797, 3807, 3811, 3821, 3829, 3833, 3855, 3857, 3863, 3865,3867, 3869, 3873, 3881, 3891, 3893, 3901, 3919, 3925, 3931, 3937, 3949,3951, 3955, 3959, 3969, 3973, 3981, 3991, 4003, 4013, 4029, 4033, 4049,4057, 4069, 4071, 4077, 4081, 4089, 4107, 4131, 4139, 4171, 4193, 4195,4199, 4201, 4223, 4225, 4245, 4249, 4273, 4279, 4281, 4287, 4293, 4301,4319, 4321, 4327, 4337, 4351, 4353, 4361, 4363, 4365, 4369, 4377, 4379,4387, 4395, 4403, 4411, 4417, 4423, 4429, 4443, 4463, 4479, 4483, 4485,4493, 4495, 4523, 4527, 4529, 4541, 4549, 4553, 4563, 4577, 4583, 4585,4595, 4619, 4635, 4651, 4677, 4683, 4685, 4699, 4703, 4709, 4711, 4719,4751, 4753, 4761, 4789, 4803, 4805, 4819, 4829, 4833, 4837, 4839, 4847,4851, 4857, 4863, 4869, 4887, 4891, 4893, 4909, 4925, 4927, 4931, 4935,4939, 4943, 4949, 4957, 4963, 4965, 4973, 5019, 5033, 5063, 5073, 5081,5083, 5085, 5089, 5093, 5113, 5167, 5179, 5183, 5193, 5209, 5211, 5213,5227, 5231, 5237, 5245, 5273, 5281, 5283, 5297, 5299, 5301, 5305, 5309,5329, 5333, 5347, 5353, 5365, 5367, 5369, 5385, 5389, 5401, 5435, 5437,5439, 5453, 5465, 5475, 5515, 5517, 5525, 5543, 5545, 5559, 5571, 5605,5611, 5619, 5639, 5651, 5655, 5665, 5685, 5701, 5715, 5761, 5769, 5771,5775, 5781, 5793, 5795, 5801, 5817, 5819, 5831, 5855, 5861, 5865, 5873,5887, 5889, 5909, 5911, 5927, 5945, 5949, 5957, 5959, 5965, 5973, 5987,5997, 6003, 6013, 6015, 6023, 6025, 6027, 6037, 6063, 6091, 6093, 6095,6097, 6111, 6117, 6119, 6135, 6143, 6147, 6165, 6177, 6187, 6189, 6191,6195, 6197, 6199, 6203, 6223, 6227, 6229, 6237, 6245, 6247, 6249, 6253,6255, 6259, 6267, 6297, 6305, 6311, 6317, 6345, 6361, 6389, 6419, 6421,6435, 6457, 6461, 6463, 6467, 6471, 6487, 6499, 6535, 6553, 6559, 6561,6565, 6617, 6631, 6633, 6643, 6677, 6699, 6707, 6733, 6745, 6781, 6783,6789, 6799, 6813, 6843, 6847, 6851, 6861, 6885, 6893, 6899, 6901, 6905,6915, 6917, 6919, 6933, 6941, 6943, 6957, 6961, 6967, 6973, 6985, 6993,6995, 7021, 7023, 7045, 7047, 7051, 7063, 7065, 7069, 7077, 7085, 7089,7091, 7097, 7099, 7107, 7113, 7123, 7129, 7131, 7167, 7191, 7199, 7219,7223, 7241, 7247, 7263, 7277, 7289, 7309, 7339, 7345, 7383, 7401, 7409,7411, 7413, 7429, 7439, 7443, 7455, 7459, 7461, 7465, 7477, 7489, 7497,7499, 7513, 7523, 7525, 7527, 7533, 7549, 7555, 7557, 7567, 7581, 7601,7611, 7615, 7617, 7623, 7633, 7641, 7651, 7653, 7659, 7665, 7687, 7693,7701, 7709, 7723, 7727, 7779, 7789, 7797, 7809, 7849, 7859, 7869, 7877,7885, 7891, 7893, 7905, 7907, 7917, 7929, 7933, 7937, 7943, 7945, 7951,7953, 7969, 7971, 8015, 8017, 8027, 8055, 8059, 8061, 8069, 8107, 8137,8145, 8149, 8151, 8153, 8171, 8179, 8217, 8219, 8231, 8239, 8245, 8247,8261, 8269, 8301, 8313, 8341, 8365, 8371, 8381, 8387, 8393, 8421, 8447,8453, 8455, 8457, 8461, 8465, 8471, 8493, 8501, 8505, 8515, 8523, 8531,8545, 8575, 8579, 8581, 8617, 8645, 8647, 8655, 8667, 8677, 8679, 8683,8689, 8691, 8697, 8709, 8715, 8721, 8727, 8731, 8739, 8745, 8775, 8793,8797, 8807, 8815, 8823, 8827, 8833, 8859, 8863, 8869, 8889, 8899, 8901,8909, 8913, 8915, 8929, 8933, 8935, 8949, 8951, 8955, 8967, 8993, 8995,8997, 9003, 9009, 9013, 9019, 9033, 9037, 9061, 9065, 9067, 9079, 9101,9103, 9123, 9153, 9157, 9163, 9185, 9189, 9197, 9201, 9223, 9225, 9227,9241, 9243, 9247, 9273, 9275, 9281, 9297, 9313, 9321, 9331, 9339, 9369,9383, 9385, 9387, 9409, 9413, 9435, 9449, 9469, 9471, 9491, 9495, 9497,9499, 9503, 9527, 9529, 9545, 9547, 9557, 9559, 9565, 9577, 9589, 9599,9601, 9603, 9605, 9607, 9621, 9633, 9635, 9653, 9655, 9663, 9681, 9701,9705, 9717, 9731, 9747, 9765, 9797, 9807, 9809, 9811, 9831, 9851, 9853,9855, 9875, 9899, 9915, 9929, 9931, 9939, 9941, 9945, 9949, 9953, 9959,9989), it will be understood that it will be translated as methioninewhen the codon functions as a start codon, but will be translated as theindicated non-Met amino acid when the sequence is at the C-terminus of afusion partner. The invention specifically discloses and encompasseseach of the amino acid sequences of the sequence listing having aN-terminus methionine residue (e.g. a formyl-methionine residue) inplace of any indicated non-Met residue (e.g. for SEQ ID NOS: 236, 662,758, 770, 782, 792, 838, 918, 1182, 1312, 1374, 1632, 2064, 2378, 2400,2456, 2516, 2636, 2670, 2682, 2688, 2744, 3384, 3702, 3714, 4058, 4678,4704, 4804, 4806, 4848, 6026, 6112, 7022, 7242, 7710, 8582, 9682). Italso specifically discloses and encompasses each of the amino acidsequences of the sequence listing starting at any internal methionineresidues in the sequences.

As indicated in the above text, nucleic acids and polypeptides of theinvention may include sequences that:

-   -   (a) are identical (i.e. 100% identical) to the sequences        disclosed in the sequence listing;    -   (b) share sequence identity with the sequences disclosed in the        sequence listing;    -   (c) have 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 single nucleotide or        amino acid alterations (deletions, insertions, substitutions),        which may be at separate locations or may be contiguous, as        compared to the sequences of (a) or (b); and    -   (d) when aligned with a particular sequence from the sequence        listing using a pairwise alignment algorithm, a moving window of        x monomers (amino acids or nucleotides) moving from start

(N-terminus or 5′) to end (C-terminus of 3′), such that for an alignmentthat extends to p monomers (where p>x) there are p-x+1 such windows,each window has at least x·y identical aligned monomers, where: x isselected from 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150, 200;y is selected from 0.50, 0.60, 0.70, 0.75, 0.80, 0.85, 0.90, 0.91, 0.92,0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99; and if x·y is is not aninteger then it is rounded up to the nearest integer. The preferredpairwise alignment algorithm is the Needleman-Wunsch global alignmentalgorithm [287], using default parameters (e.g. with Gap openingpenalty=10.0, and with Gap extension penalty=0.5, using the EBLOSUM62scoring matrix). This algorithm is conveniently implemented in theneedle tool in the EMBOSS package [288].

The nucleic acids and polypeptides of the invention may additionallyhave further sequences to the N-terminus/5′ and/or C-terminus/3′ ofthese sequences (a) to (d).

The practice of the present invention will employ, unless otherwiseindicated, conventional methods of chemistry, biochemistry, molecularbiology, immunology and pharmacology, within the skill of the art. Suchtechniques are explained fully in the literature. See, e.g., references289-296, etc.

EXPERIMENTAL

Below are examples of specific embodiments or modes for carrying out thepresent invention. The examples are offered for illustrative purposesonly, and are not intended to limit the scope of the present inventionin any way.

Efforts have been made to ensure accuracy with respect to numbers used(e.g., amounts, temperatures, etc.), but some experimental error anddeviation should, of course, be allowed for.

MODES FOR CARRYING OUT THE INVENTION MNEC Strain IHE3034

IHE3034 is a known E. coli strain of the MNEC pathotype. Table 1 ofreference 297 reports that IHE3034 is classified as O18:K1:H7/9serotype, and falls into ECOR group B2. Known virulence-associated geneson horizontally transferred DNA elements are sfall, ibeA, iro, kps(group II), fyuA and malX. It also carries the cdt gene cluster codingfor the cytolethal distending toxin.

Sequencing of E. coli K1 strain IHE3034 led to 283 contigs. These aregiven as SEQ ID NOs 9991 to 10273, arranged in descending order oflength.

Analysis of the genome sequence of E. coli K1 strain IHE3034 hasidentified 4995 open reading frames (ORFs), which are referred to by thenomenclature ORFnnnnn, where nnnnn is a number between 00001 and 04995.Sequences for these ORFs are given as SEQ ID NOs: 1 to 9990, withodd-numbered SEQ ID NOs being DNA sequences, and even-numbered IDs beingamino acid sequences. The nnnnn numbering can be converted to the SEQ IDNO: numbering of the sequence listing as follows: for an amino acidsequence, SEQ ID NO: =nnnnn×2; for a nucleotide sequence, SEQ ID NO:=[nnnnn×2]−1. Thus ORF01234 is found as SEQ ID NOs: 2467 & 2468, asshown in Table 1.

Initial functional annotation of the 4995 ORFs is given in Table 1.

Table 3 shows the ORFs which have low homology with commensal strainORFs (1194 in total). These ORFs are preferred for giving specificity topathological E. coli strains vs. commensal strains, both in relation tonucleic acid testing and in relation to immunological cross-reactity.Potential pathogenicity islands are located in the ranges ORF00028-70,ORF000330-353, ORF00655-687, ORF00883-910, ORF01031-1058, ORF1078-1119,ORF1186-1228, ORF01313-1376, ORF01479-1514, ORF01523-1543,ORF01550-1578, ORF01810-1865, ORF02295-02315, ORF02351-2375,ORF02382-2405, ORF02436-2472, ORF02788-02816, ORF02844-2891,ORF03011-3056, ORF03340-3365, ORF03499-3522, ORF04416-4439,ORF04661-4679, ORF04915-4930, and ORF04946-4966. Based on variouscriteria applied by the inventors, a subset of 142 of the 4995 ORFs(2.8%) has been selected for immunogenic use. These 142 ORFs are listedin Table 2. The criteria for selection of the subset include, but arenot limited to: low homology with ORFs from commensal E. coli strains;length >100aa; and suitable cellular localisation (shown at bottom ofTable 2).

The genes encoding these 142 proteins are cloned, expressed in bacteria(e.g. in a non-pathogenic laboratory E. coli host, or in a Bacillus suchas B. subtilis or B. megaterium), purified, and then are used toimmunise test animals (e.g. mice). The serum raised in the mice are thenanalysed in Western blot, ELISA and FACS assays, and are further testedin both in vitro and in vivo experiments. Suitable in vitro experimentsinclude testing the ability of antibodies to induce complement-mediatedbacterial killing and/or opsonophagocytosis activity, to block bindingof MNEC strains (or the purified antigen) to human epithelial cells orother cell lines, and/or to inhibit adhesion/invasion of E. colibacteria (e.g. K1 strain) to brain microvascular endothelial cells(BMEC). Suitable in vivo experiments for testing efficacy againstbacteremia and meningitis include active and/or passive systemicimmunisations and challenge in 5-day-old rats challenged with E. coli K1strain, and immunisation and intraperitoneal infection of adult micewith MNEC strains.

The importance of the proteins to the bacterial life-cycle can be testedby creating isogenic knockout mutants. The mutants can also be used toensure that sera raised by an antigen are specific for that antigen.Microarrays are used to study expression patterns. Conservation and/orvariability is assessed by sequencing the genes from multiple differentExPEC strains.

Assays were carried out in order to select predicted surface-exposedproteins, which are specific for MNEC strains and absent innon-pathogenic strains (commensal and laboratory strains). Once selectedthese proteins are expressed and purified and used to immunize mice.

It is known from reference 43 that a mutation in any of the tol-palgenes of E. coli results in the formation of vesicles containing nativeouter membrane proteins. By comparing the proteins present in vesiclesof MNEC strains and non-pathogenic strains it is possible to select asmall group of proteins that could be used as potential antigens.

Lambda Red-Mediated Gene Manipulation in Commensal and Pathogenic E.coli

This method is a rapid PCR-based method used to inactivate the tolR genefrom the wild-type E. coli strains [298]. Briefly, the first stepconsists in amplifying independently the upstream and downstream regionsof the target gene (tolR) and the resistance marker cassette. The twoPCR products obtained in step 1 are mixed with the amplificationproducer of the AB cassette at equimolar concentrations and submitted toa second round of PCR (a three way PCR) to generate a resistance markercassette flanked by upstream and downstream 500 bp (or more) regionshomologous to the target gene. In the third step, large amounts (1 μg)of the desired linear DNA are electroporated into lamda-red competentcells.

Vesicle Preparation

1. Vesicle Preparation by Precipitation with TCA

LB media was inoculated with bacteria grown on plates and incubatedovernight at 37° C. under gentle shaking. The culture was used toinoculate 200 ml of LB at OD600 0.1. Bacteria were grown to OD600 0.4(or as specified). Culture was centrifuged for 10 minutes at 4000×g andthe supernatant was filtered through a 0.22 mm filter to remove residualbacteria.

The same experiments were also performed under iron limiting conditionsby adding Dipyridyl (0.25 mM) to the LB media.

Precipitation was performed by adding to the culture supernatant 10%final of a solution at 100% (w/v) TCA, 0.4% (w/v) deoxycholate. Theprecipitation was allowed to proceed for 30 minutes at 4° C. Precipitatewas recovered by 10 minutes centrifugation at 20000×g at 4° C. Thepellet was washed once with 10% TCA (w/v) and twice with absoluteethanol. The pellet was dried with speed vac, and stored at −20° C.

The wild type and mutated strains were subjected to SDS polyacrylamidegel electrophoresis from which it could be observed that there were manymore bands in the supernatant of the mutated strains than the wildtypestrains. Randomly picked bands demonstrated that all the proteins in thesupernatant were membrane proteins.

2. Vesicle Preparation by Ultracentrifugation

Culture supernatant was ultracentrifuged at 200000×g for 2 hours at 4°C. The pellet was washed with PBS, resuspended in PBS, and stored at−20° C.

3. Guanidinium Denaturation of the Vesicles

Prior to the guanidinium denaturation, Vesicles were precipitated withethanol. 10 μg of OMV in PBS were precipitate by adding cold absoluteethanol to 90% final. Precipitation was allowed to proceed for 20minutes at −20° C. Precipitate was recovered by 10 minutescentrifugation at 13000×g. Pellet was resuspended with 50 ml, 6Mguanidinium, 15 mM DTT, 200 mM Tris-HCl, pH 8.0. Denaturation wasallowed to proceed for 60 minutes at 60° C. Prior to digestion, solutionwas diluted 1/8 with a solution of 1.5M Tris pH 8.0 and 5 mg of trypsinwere added to the diluted solution. Digestion was allowed to proceedovernight at 37° C. Reaction was stopped by adding 0.1% final of formicacid. Peptides were extracted using Oasis extraction cartridges.Peptides were analyzed by LC coupled MS-MS.

4. Surface Digestion

5 mg of trypsin were added to 10 mg of vesicles in PBS and incubated at37° C. for 3 hours. Reaction was stopped by adding 0,1% final of formicacid. Peptides were recovered by filtration through a 30 Kda cut-offfilter and extracted with Oasis extraction cartridge. Peptides wereanalyzed with LC coupled MSMS.

Vesicle Analysis

Protein Quantification

Proteins were quantified with the Bradford method, using the BSA asstandard.

SDS-PAGE

Samples were analyzed with a sodium dodecyl sulfate (SDS) 4-12%polyacrylamide gel, using a Mini-Protean II electrophoresis apparatus.Samples were suspended in SDS sample buffer (0.06 M Tris-HCl pH 6.8, 10%(v/v) glycerol, 2% (w/v) SDS, 5% (v/v) 2-mercaptoethanol, 10 mg/mlbromophenol blue) and heated to 100° C. for 5 min beforeSDS-polyacrylamide gel electrophoreis. After the run, gels were stainedwith Coomassie Blue

MALDI-TOF Mass Spectrometry.

Protein bands or spots were excised from gels, washed with 50 mMammonium bicarbonate/acetonitrile (50/50, v/v), and dried with aSpeed-Vac centrifuge (Savant). The dried spots were digested at 37° C.for 2 h by adding 7 to 10 ml of a solution containing 5 mM ammoniumbicarbonate, 0.012 mg of sequencing-grade trypsin. After digestion 0.6ml were loaded on a matrix pre-spotted target and air-dried. Spots werewashed with 0.6 ml of a solution of 70% ethanol, 0.1% trifluoraceticacid. Mass spectra were acquired on an ultraflex MALDI TOF massspectrometer. Spectra were externally calibrated by using a combinationof standards pre-spotted on the target. Protein identification wascarried out by both automatic and manual comparisons of experimentallygenerated monoisotopic peaks of peptides in the mass range of 700 to3,000 Da with computer-generated fingerprints, using the Mascot program.

Bi-Dimensional Electrophoresis

200 mg of vesicles were resuspended in an Immobiline re-swellingsolution (7M urea, 2M thiourea, 2% (w/v) CHAPS (2% w/v) ASB14, 2% (v/v)IPG buffer pH 3-10 NL, 2 mM TBP, 65 mM DTT), and adsorbed overnight on 7cm Immobiline DryStrips (pH 3-10 NL). Proteins were then separated by 2Delectrophoresis. The first dimension was run using a IPGphor IsoelectricFocusing Unit, applying sequentially 150 V for 35 minutes, 500 V for 35minutes, 1,000 V for 30 minutes, 2,600 V for 10 minutes, 3,500 V for 15minutes, 4,200 V for 15 minutes, and finally 5,000 V to reach 10 kVh.For the second dimension, the strips were equilibrated by two 10minute-incubations in 4 M urea, 2 M thiourea, 30% glycerol, 2% SDS, 5 mMTBP, 50 Mm Tris HCl pH 8.8, 2.5% acrylamide, Bromo phenol Blue 0.2%:Proteins were then separated on linear 4-12% precasted polyacrylamidegels.

Gels were stained with colloidal Coomassie Blue and scanned with aPersonal Densitometer SI. Images were analyzed with Image Master 2DElite software.

Nano-LC/MS/MS

Peptides were separated by nano-LC on a CapLC HPLC system connected to aQ-ToF Micro ESI mass spectrometer equipped with a nanospray source.Samples were loaded onto an Atlantis C18 NanoEase column (100 μmi.d.×100 mm), through a C18 trap column (300 μm i.d.×5 mm) Peptides wereeluted with a 50-min gradient from 2% to 60% of 95% ACN, in a solutionof 0.1% formic acid at a flow rate of 400 nl/minute. The eluted peptideswere subjected to an automated data-dependent acquisition program, usingthe MassLynx software, version 4.0, where a MS survey scan was used toautomatically select multi-charged peptides over the m/z range of400-2,000 for further MS/MS fragmentation. Up to three differentcomponents where subjected to MS/MS fragmentation at the same time.After data acquisition, the individual MS/MS spectra were combined,smoothed and centroided by MassLynx. Search and identification ofpeptides were performed in batch mode with a licensed version of MASCOT.The MASCOT search parameters were: (1) species: ExPEC (2) allowed numberof missed cleavages (only for trypsin digestion): 6; (3) variablepost-translational modifications: methionine oxidation; (4) peptidetolerance: ±500 ppm; (5) MS/MS tolerance: ±0.3 Da and (6): peptidecharge: from +1 to +4. As for the previous platform, only significanthits as defined by MASCOT probability analysis were considered. Thescore thresholds for acceptance of protein identifications from at leastone peptide were set by MASCOT as 18 for trypsin digestion and 36 forproteinase K digestion.

Results

As a result of the above analyses, 10 further preferred antigens wereidentified. Namely orf03526 (SEQ ID No: 7051+7052), orf01339 (SEQ ID No:2677+2678), orf00256 (SEQ ID No: 511+512), orf01346 (SEQ ID No:2691+2692), orf04084 (SEQ ID No: 8167+8168), orf02374 (SEQ ID No:4747+4748), orf03502 (SEQ ID No: 7003+7004), orf02298 (SEQ ID No:4595+4596), orf01228 (SEQ ID No: 2455+2456), orf01227 (SEQ ID No:2453+2454), orf02314 (SEQ ID NO: 4627+4628) and orf02850 (SEQ ID NO:5699+5700). These are listed in Table 4 where the identities of theseproteins to proteins in the UPEC strain CFT073 are also given. Furtherpreferred antigens are those which display greater than 60% identity topreferred antigens from the CFT073 strain which were identified usingthe same methods as above. These preferred antigens are orf04282 (SEQ IDNo: 8563+8564), orf01364 (SEQ ID No: 2727+2728), orf04936 (SEQ ID No:9871+9872), orf02336 (SEQ ID No: 4671+4672), orf02840 (SEQ ID No:5679+5680), orf00267 (SEQ ID No: 533+534), orf01611 (SEQ ID No:3221+3222), orf01613 (SEQ ID No: 3225+3226) and orf03502 (SEQ ID No:7003+7004).

Antigen Analysis

Mouse Model of Systemic Infection

To screen a large number of antigens selected by comparative genomeanalysis between pathogenic and non pathogenic E. coli strains, aprotection model based on a classical virulence assay has beenestablished.

The experimental model (immunization and infection) uses 5 week old-CD1outbreed mice which are challenged with intraperitoneal inoculation ofvirulent IHE3034 E. coli strain. The challenge dose has beenexperimentally determined as the amount of bacteria able to kill 80% ofadult mice within 72 hours and corresponds to 1×10⁷ cfu/mouse for theIHE3034 strain.

Two proteins, described as virulence factors and potential protectiveantigens were used as positive controls. These proteins are the IbeA[299] and the IroN (SEQ ID No:2691, 2692 and refs 300,301). Therecombinant proteins were expressed and used in the immunization assays.

Immunization Protocol

Mice are immunized three times by subcutaneous injection of 150 μl ofprotein solution using freund's adjuvants as shown in the table below:

Control mice: Immunized mice: Day 0 75 μl of saline solution 75 μl ofprotein solution (20 μg) 75 μl of complete freund′s 75 μl of completefreund′s adjuvant adjuvant Day 21 75 μl of saline solution 75 μl ofprotein solution (20 μg) 75 μl of incomplete freund′s 75 μl ofincomplete freund′s adjuvant adjuvant Day 35 75 μl of saline solution 75μl of protein solution (20 μg) 75 μl of incomplete freund′s 75 μl ofincomplete freund′s adjuvant adjuvant

Blood samples are colleccted the day before the first immunization(preimmune serum), at day 34 and 48 (day before challenge). Sera fromimmunized animals are tested by western blot and ELISA to determine theantibodies titer.

Challenge

At day 48 E. coli IHE3034 strain is streaked on LB agar plate fromfrozen stock and incubated overnight (ON) at 37° C. in incubator. At Day49 the ON plate-culture is used to inoculate 50 ml of LB medium to havean O.D.₆₀₀=0.1, and grown for 1.5 hours at 37° C. under agitation untilthe bacterial culture reaches an O.D.₆₀₀=0.6 corresponding to 5×10⁸cfu/ml for the IHE3034 strain. The culture is diluted in physiologicalsolution until the concentration of bacteria is 1×10⁸/ml (typically 2 mlof bacterial culture is diluted in 8 ml of physiological solution) andplated using a standard plate count method to verify the inoculum. 100μl of the cell suspension containing 1×10⁷ IHE3034 bacteria is injectedintraperitoneally, using a 1 ml syringe, to control and immunized mice.The number of deaths in each animal group at 24, 48 and 72 hours afterinfection are recorded.

The protection due to vaccination is evaluated by comparison of thesurvival in the vaccinated group and the survival in control group ofmice at 72 hours from the challenge. Percentage of survival relative tocontrols is calculated using the formula:

$\frac{\begin{matrix}{{{rate}\mspace{14mu} {of}\mspace{14mu} {survival}\mspace{14mu} {in}\mspace{14mu} {vaccine}\mspace{14mu} {group}} -} \\{{rate}\mspace{14mu} {of}\mspace{14mu} {survival}\mspace{14mu} {in}\mspace{14mu} {control}\mspace{14mu} {group}}\end{matrix}}{{rate}\mspace{14mu} {of}\mspace{14mu} {survival}\mspace{14mu} {in}\mspace{14mu} {control}\mspace{14mu} {group}}$

Results

Immunization was carried out as above with positive controls IroN andIbeA. As can be seen in FIGS. 3 and 4 the % survival of the mice afterchallenge with IHE3034 is increased following immunization with eitherIroN or IbeA.

Immunization was then carried out with heat-inactivated IHE3034. As canbe seen in FIG. 1, the % survival of the mice after challenge withIHE3034 is increased following immunization with heat-inactivatedIHE3034.

Immunization was also carried out with vesicles from IHE3034 ΔTol-R. Ascan be seen in FIG. 2, the % survival of the mice after challenge withIHE3034 is increased following immunization with IHE3034 ΔTol-R.

Immunisation Studies

Antigens are selected for combining to give a composition of theinvention. BALB/c mice are divided into nine groups and immunized asfollows:

Group Immunizing Composition Route of Delivery 1 Mixture of antigensIntra-peritoneal (10-20 μg protein/each) + or intra-nasal CFA (CompleteFreund′s Adjuvant) or subcutaneous 2 Mixture of antigensIntra-peritoneal (5 μg/each) + Al-hydroxide or intra-nasal (200 μg) orsubcutaneous 3 Mixture of antigens Intra-peritoneal (10-20 μgprotein/each) + or intra-nasal CpG (10 ug) or subcutaneous 4 Mixture ofantigens Intra-peritoneal (10-20 μg protein/each) + or intra-nasalAl-hydroxide (200 μg) + or subcutaneous CpG (10 μg) 5 CFAIntra-peritoneal or intra-nasal or subcutaneous 6 Mixture of antigensIntra-peritoneal (10-20 μg protein/each) + or intra-nasal LTK63 (5 μg)or subcutaneous 7 Al-hydroxide Intra-peritoneal (200 μg) + CpG (10 μg)or intra-nasal or subcutaneous 8 CpG (10 μg) Intra-peritoneal orintra-nasal or subcutaneous 9 LTK63 (5 μg) Intra-peritoneal orintra-nasal or subcutaneous

Mice are immunized at two-week intervals. Two to three weeks after thelast immunization, all mice are challenged with the appropriate MNECstrain. When mucosal immunization (e.g. intranasal) is used, the animalmodel is also challenged mucosally to test the protective effect of themucosal immunogen Immediately prior to challenge, mice are bled todetermine antibody titre to the antigens that were administered.

For the mouse challenge, virulent bacteria will be grown in appropriatemedia. Bacteria are harvested by centrifugation, re-suspended, andserially diluted for the challenge inoculum. BALB/c mice are challengedand observed daily for 30 days post-exposure.

Total IgG and IgG1/IgG2A subtypes can be measured in mouse seraresulting from the different immunization regimens by using an ELISAassay on whole bacteria and on purified recombinant proteins.Furthermore, assessment of antigen-specific CD4+ and CD8+ Th-cells inspleen cells and/or PBMC isolated from immunized mice can be carried outby multi-parametric FACS analysis, to evaluate the cytokine expressionprofiles of antigen-specific T-cells. In particular production of IFN-γand IL-5 can be measured after in vitro stimulation of T cells withpurified antigens. In addition, splenocytes and/or PBMC from miceimmunized with each antigen/vaccine formulation may be collected 10-12days after the last immunization dose and stimulated with MNEC bacteria.After 4 hours of stimulation, Brefeldin A is added to the cells for thefollowing 12 hours, to block cytokine secretion. Afterwards cells arefixed and stained with antibodies to detect MNEC-specific T cellsexpressing IFN-γ and IL-5.

T cells can be isolated from peripheral blood lymphocytes (PBLs) by avariety of procedures known to those skilled in the art. For example, Tcell populations can be “enriched” from a population of PBLs through theremoval of accessory and B cells. In particular, T cell enrichment canbe accomplished by the elimination of non-T cells using anti-MHC classII monoclonal antibodies. Similarly, other antibodies can be used todeplete specific populations of non-T cells. For example, anti-Igantibody molecules can be used to deplete B cells and anti-MacI antibodymolecules can be used to deplete macrophages.

T cells can be further fractionated into a number of differentsubpopulations by techniques known to those skilled in the art. Twomajor subpopulations can be isolated based on their differentialexpression of the cell surface markers CD4 and CD8. For example,following the enrichment of T cells as described above, CD4+ cells canbe enriched using antibodies specific for CD4. The antibodies may becoupled to a solid support such as magnetic beads. Conversely, CD8+cells can be enriched through the use of antibodies specific for CD4 (toremove CD4+ cells), or can be—isolated by the use of CD8 antibodiescoupled to a solid support. CD4 lymphocytes from MNEC-infected patientscan be expanded ex vivo, before or after transduction.

Following purification of T cells, the purified T cells arepre-stimulated with various cytokines including but not limited torIL-2, IL-10, IL-12, and IL-15, which promote growth and activation oflymphocytes.

MNEC-specific T cells, may be activated by the above describedimmunogenic polypeptides. MNEC-specific T cells can be CD8+ or CD4+.MNEC-specific CD8+ T cells can be cytotoxic T lymphocytes (CTL) whichcan kill MNEC-infected cells that display any of the above describedpolypeptides or fragments thereof complexed with an MHC class Imolecule. Chlamydia-specific CD8+ T cells can be detected by, forexample, ⁵¹Cr release assays. ⁵¹Cr release assays measure the ability ofMNEC-specific CD8+ T cells to lyse target cells displaying one or moreof these epitopes. MNEC-specific CD8+ T cells which express antiviralagents, such as IFN γ, are also contemplated herein and can also bedetected by immunological methods, preferably by intracellular stainingfor IFN-γ or alike cytokines after in vitro stimulation with one or moreof the above described MNE polypeptides. MNEC-specific CD4+ T cells canbe detected by a lymphoproliferation assay. Lymphoproliferation assaysmeasure the ability of MNEC-specific CD4+ T cells to proliferate inresponse to one or more of the above described polypeptides.

It will be understood that the invention has been described by way ofexample only and modifications may be made whilst remaining within thescope and spirit of the invention.

TABLE 1 Annotation of ORF00001 to ORF04995 ORFnnnnn SEQ ID NOsAnnotation ORF00001 1 & 2 Phosphoglycerol transferase I ORF00002 3 & 4primosomal operon 17.5K protein (mdob-dnac intergenic region) ORF00003 5& 6 DNA replication protein dnaC ORF00004 7 & 8 Primosomal protein IORF00005 9 & 10 probable integral membrane protein Cj1165c, putativeORF00006 11 & 12 probable integral membrane protein Cj1166c, putativeORF00007 13 & 14 transcriptional regulator, LuxR family domain proteinORF00008 15 & 16 Transcriptional activator protein bglJ ORF00009 17 & 18Ferric iron reductase protein fhuF [1.6.99.—] ORF00010 19 & 20 Proteinof unknown function (DUF1435) family ORF00011 21 & 22 conservedhypothetical protein ORF00012 23 & 24 Ribosomal RNA small subunitmethyltransferase C (AE005668) [2.1.1.52] ORF00013 25 & 26 DNApolymerase III, psi subunit (holD) [2.7.7.7] ORF00014 27 & 28ribosomal-protein-alanine acetyltransferase (rimI) [2.3.1.128] ORF0001529 & 30 HAD superfamily (subfamily IA) hydrolase, TIGR02254 ORF00016 31& 32 site-specific recombinase, phage integrase family ORF00017 33 & 34hypothetical protein ORF00018 35 & 36 conserved hypothetical proteinORF00019 37 & 38 Hypothetical nin region protein (cysD) ORF00020 39 & 40hypothetical protein ORF00021 41 & 42 conserved hypothetical proteinORF00022 43 & 44 Sb36 ORF00023 45 & 46 Sb36 ORF00024 47 & 48hypothetical protein ORF00025 49 & 50 hypothetical protein ORF00026 51 &52 conserved hypothetical protein ORF00027 53 & 54 repressor ORF00028 55& 56 Sb40 ORF00029 57 & 58 immunity region ORF00030 59 & 60 conservedhypothetical protein ORF00031 61 & 62 replication protein ORF00032 63 &64 hypothetical protein ORF00033 65 & 66 phageN-6-adenine-methyltransferase [2.1.1.72] ORF00034 67 & 68 LexA repressorORF00035 69 & 70 crossover junction endodeoxyribonuclease [3.1.22.—]ORF00036 71 & 72 Sb46 ORF00037 73 & 74 Protein of unknown function(DUF1277) superfamily ORF00038 75 & 76 Antitermination protein Q homologfrom lambdoid prophage ORF00039 77 & 78 lysis protein S.b1556 ORF0004079 & 80 endolysin (lysozyme) [3.2.1.17] ORF00041 81 & 82 Bacteriophagelysis protein ORF00042 83 & 84 hypothetical protein ORF00043 85 & 86Gifsy-2 prophage protein ORF00044 87 & 88 Phage terminase large subunit(GpA) ORF00045 89 & 90 conserved hypothetical protein ORF00046 91 & 92phage portal protein, lambda family ORF00047 93 & 94 Gifsy-2 prophageresembles Clp protease ORF00048 95 & 96 conserved hypothetical proteinORF00049 97 & 98 Gifsy-2 prophage ATP-binding sugar transporter-likeprotein ORF00050 99 & 100 Gifsy-1 prophage: similar to minor tailprotein Z ORF00051 101 & 102 Phage minor tail protein U ORF00052 103 &104 putative tail component of prophage CP-933K ORF00053 105 & 106 phageminor tail protein G ORF00054 107 & 108 phage tail assembly protein TORF00055 109 & 110 Gifsy-1 prophage protein ORF00056 111 & 112 Phageminor tail protein ORF00057 113 & 114 phage minor tail protein LORF00058 115 & 116 Putative tail fiber component K of prophage ORF00059117 & 118 Putative tail fiber component K of prophage ORF00060 119 & 120Gifsy-1 prophage protein ORF00061 121 & 122 Gifsy-1 prophage proteinORF00062 123 & 124 host specificity protein (partial) ORF00063 125 & 126Lom ORF00064 127 & 128 conserved hypothetical protein ORF00065 129 & 130hypothetical protein ORF00066 131 & 132 conserved hypothetical proteinORF00067 133 & 134 conserved hypothetical protein ORF00068 135 & 136conserved hypothetical protein ORF00069 137 & 138 maturase-relatedprotein ORF00070 139 & 140 DinI-like protein Z3916-ECs3483 (AF175466)ORF00071 141 & 142 peptide chain release factor 3 (prfC) ORF00072 143 &144 Osmotically inducible protein Y precursor ORF00073 145 & 146 Proteinof unknown function (DUF1328) family ORF00074 147 & 148 unnamed proteinproduct ORF00075 149 & 150 Mg-dependent DNase ORF00076 151 & 152pyruvate formate lyase activating enzyme (act) [1.97.1.4] ORF00077 153 &154 Protein ORF00078 155 & 156 conserved hypothetical protein ORF00079157 & 158 deoxyribose-phosphate aldolase (deoC) [4.1.2.4] ORF00080 159 &160 Thymidine phosphorylase (TDRPASE) ORF00081 161 & 162phosphopentomutase (deoB) [5.4.2.7] ORF00082 163 & 164 purine nucleosidephosphorylase (deoD) [2.4.2.1] ORF00083 165 & 166 conserved hypotheticalprotein ORF00084 167 & 168 lipoate-protein ligase A [6.3.4.—] ORF00085169 & 170 Protein smp precursor ORF00086 171 & 172 phosphoserinephosphatase (serB) [3.1.3.3] ORF00087 173 & 174 DNA repair protein RadA(radA) ORF00088 175 & 176 nicotinamide-nucleotide adenylyltransferase[2.7.7.1] ORF00089 177 & 178 conserved hypothetical protein ORF00090 179& 180 Helix-turn-helix motif ORF00091 181 & 182 ABC transporterATP-binding protein yjjK (atp_bind) ORF00092 183 & 184 soluble lyticmurein transglycosylase [3.2.1.—] ORF00093 185 & 186 trp operonrepressor (trpR) ORF00094 187 & 188 conserved hypothetical proteinTIGR00258 ORF00095 189 & 190 phosphoglycerate mutase family protein,putative [5.4.2.1] ORF00096 191 & 192 Right origin-binding proteinORF00097 193 & 194 CreA protein ORF00098 195 & 196 TranscriptionalRegulatory protein creB ORF00099 197 & 198 Sensor protein creC [2.7.3.—]ORF00100 199 & 200 Inner membrane protein creD (AE005671) ORF00101 201 &202 Aerobic respiration control protein arcA (Dye resistance protein)(arcA) ORF00102 203 & 204 RNA methyltransferase, TrmH family, group 1ORF00103 205 & 206 aspartokinase I, homoserine dehydrogenase I ORF00104207 & 208 homoserine kinase (thrB) [2.7.1.39] ORF00105 209 & 210threonine synthase (thrC) [4.2.3.1] ORF00106 211 & 212 unnamed proteinproduct; ORF_ID: o101#5 ORF00107 213 & 214 conserved hypotheticalprotein ORF00108 215 & 216 UPF0246 protein yaaA ORF00109 217 & 218unnamed protein product (ORF8) ORF00110 219 & 220 transaldolase (tal)[2.2.1.2] ORF00111 221 & 222 Molybdopterin biosynthesis mog proteinORF00112 223 & 224 gpr1-fun34-yaaH family protein ORF00113 225 & 226unnamed protein product; ORF4 ORF00114 227 & 228 conserved hypotheticalprotein ORF00115 229 & 230 hypothetical protein ORF00116 231 & 232chaperone protein DnaK (dnaK) ORF00117 233 & 234 chaperone protein DnaJ(dnaJ) ORF00118 235 & 236 gef protein-related protein ORF00119 237 & 238Putative conserved protein ORF00120 239 & 240 Sulfatase, putativeORF00121 241 & 242 conserved hypothetical protein ORF00122 243 & 244Na+—H+ antiporter NhaA (nhaA) ORF00123 245 & 246 Transcriptionalactivator protein nhaR ORF00124 247 & 248 riboflavin biosynthesisprotein RibF (ribF) [2.7.1.26 2.7.7.2] ORF00125 249 & 250 isoleucyl-tRNAsynthetase (ileS) [6.1.1.5] ORF00126 251 & 252 lipoprotein signalpeptidase (lspA) [3.4.23.36] ORF00127 253 & 254 5.2.1.8 [5.2.1.8]ORF00128 255 & 256 4-hydroxy-3-methylbut-2-enyl diphosphate reductase(ispH) [1.17.1.2] ORF00129 257 & 258 inosine-uridine preferringnucleoside hydrolase [3.2.—.—] ORF00130 259 & 260 hyopthetical proteinDUF805 ORF00131 261 & 262 dihydrodipicolinate reductase (dapB)[1.3.1.26] ORF00132 263 & 264 carbamoyl-phosphate synthase, smallsubunit (carA) [6.3.5.5] ORF00133 265 & 266 carbamoyl-phosphatesynthase, large subunit (carB) [6.3.5.5] ORF00134 267 & 268 TransposaseORF00135 269 & 270 Transcriptional activatory protein caiF ORF00136 271& 272 Carnitine operon protein caiE ORF00137 273 & 274 echA1 [5.—.—.—]ORF00138 275 & 276 caiC (fadD) [6.3.2.—] ORF00139 277 & 278 CAIB-BAIFfamily protein, putative [2.8.3.—] ORF00140 279 & 280Crotonobetainyl-CoA dehydrogenase (Crotonobetainyl-CoAreductase) (acd)[1.3.99.—] ORF00141 281 & 282 L-carnitine-gamma-butyrobetaine antiporterORF00142 283 & 284 electron transfer flavoprotein, beta subunit,putative ORF00143 285 & 286 electron transfer flavoprotein, alphasubunit, putative ORF00144 287 & 288 FixC protein [1.5.5.—] ORF00145 289& 290 Ferredoxin-like protein ORF00146 291 & 292 sugar transporter,putative ORF00147 293 & 294 Glutathione-regulated potassium-effluxsystem ancillary protein kefF (QUINONE) [1.6.99.2] ORF00148 295 & 296Glutathione-regulated potassium-efflux system protein kefC(K(+)—H(+)antiporter) ORF00149 297 & 298 dihydrofolate reductase (folA)[1.5.1.3] ORF00150 299 & 300 CcdB antidote CcdA ORF00151 301 & 302CcdB-like protein ORF00152 303 & 304 CcdB-like protein ORF00153 305 &306 bis(5′-nucleosyl)-tetraphosphatase (symmetrical) [3.6.1.41] ORF00154307 & 308 Protein apaG (AJ012295) ORF00155 309 & 310 dimethyladenosinetransferase (ksgA) [2.1.1.—] ORF00156 311 & 3124-hydroxythreonine-4-phosphate dehydrogenase (pdxA) [1.1.1.262] ORF00157313 & 314 Survival protein surA precursor (Peptidyl-prolyl cis-transisomerasesurA) (PPlase) (Rotamase C) (PPlase) [5.2.1.8] ORF00158 315 &316 Organic solvent tolerance protein precursor ORF00159 317 & 318Organic solvent tolerance protein precursor ORF00160 319 & 320 DnaJ-likeprotein djlA (AE005182) ORF00161 321 & 322 ribosomal large subunitpseudouridine synthase A ORF00162 323 & 324 RNA polymerase associatedprotein (hepA) [3.6.1.—] ORF00163 325 & 326 hypothetical proteinORF00164 327 & 328 DNA polymerase II ORF00165 329 & 330L-ribulose-5-phosphate 4-epimerase (araD) [5.1.3.4] ORF00166 331 & 332L-arabinose isomerase (araA) [5.3.1.4] ORF00167 333 & 334 L-ribulokinase(araB) [2.7.1.16] ORF00168 335 & 336 transcriptional regulator, AraCfamily ORF00169 337 & 338 conserved hypothetical protein ORF00170 339 &340 DedA family integral membrane protein ORF00171 341 & 342 thiamineABC transporter, ATP-binding protein ORF00172 343 & 344thiamine-thiamine pyrophosphate ABC transporter, permease protein (thiP)ORF00173 345 & 346 thiamin-thiamin pyrophosphate ABC transporter,thiamin-thiamin pyrophospate- binding protein (thiB) ORF00174 347 & 348Bacterial extracellular solute-binding proteins, family 5 familyORF00175 349 & 350 3-isopropylmalate dehydratase, small subunit (leuD)[4.2.1.33] ORF00176 351 & 352 3-isopropylmalate dehydratase, largesubunit (leuC) [4.2.1.33] ORF00177 353 & 354 3-isopropylmalatedehydrogenase (leuB) [1.1.1.85] ORF00178 355 & 356 2-isopropylmalatesynthase (leuA) [2.3.3.13] ORF00179 357 & 358 LeuO protein. ORF00180 359& 360 acetolactate synthase, large subunit, biosynthetic type (ilvB)[2.2.1.6] ORF00181 361 & 362 acetolactate synthase, small subunit (ilvN)[2.2.1.6] ORF00182 363 & 364 transcriptional repressor of fru operon andothers ORF00183 365 & 366 mraZ protein (mraZ) ORF00184 367 & 368S-adenosyl-methyltransferase MraW (mraW) [2.1.1.—] ORF00185 369 & 370Cell division protein ftsL ORF00186 371 & 372 Peptidoglycan synthetaseftsI precursor (PBP) [2.4.1.129] ORF00187 373 & 374UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligaseORF00188 375 & 376UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanylligase (murF) [6.3.2.10] ORF00189 377 & 378phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) [2.7.8.13]ORF00190 379 & 380 UDP-N-acetylmuramoylalanine--D-glutamate ligase(murD) [6.3.2.9] ORF00191 381 & 382 Cell division protein ftsW ORF00192383 & 384 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (murG) [2.4.1.227] ORF00193 385 & 386UDP-N-acetylmuramate-alanine ligase (murC) [6.3.2.8] ORF00194 387 & 388D-alanine--D-alanine ligase B (ddlB) [6.3.2.4] ORF00195 389 & 390Acetolactate synthase isozyme III large subunit [4.1.3.18] ORF00196 391& 392 acetolactate synthase, small subunit (ilvN) [2.2.1.6] ORF00197 393& 394 fructose repressor (catabolite repressor-activator) ORF00198 395 &396 fructose repressor (catabolite repressor-activator) ORF00199 397 &398 Acetolactate synthase isozyme III large subunit [4.1.3.18] ORF00200399 & 400 acetolactate synthase, small subunit (ilvN) [2.2.1.6] ORF00201401 & 402 fructose repressor (catabolite repressor-activator) ORF00202403 & 404 D-alanine-D-alanine ligase B (ddlB) [6.3.2.4] ORF00203 405 &406 cell division protein ftsQ ORF00204 407 & 408 cell division proteinFtsA (ftsA) ORF00205 409 & 410 Cell division protein ftsZ ORF00206 411 &412 UDP-3-0-acyl N-acetylglucosamine deacetylase (lpxC) [3.5.1.—]ORF00207 413 & 414 Secretion monitor precursor ORF00208 415 & 416 secAprotein ORF00209 417 & 418 preprotein translocase, SecA subunit (secA)ORF00210 419 & 420 Mutator mutT protein (mutT) [3.6.1.—] ORF00211 421 &422 UPF0243 zinc-binding protein yacG-related protein ORF00212 423 & 424Hypothetical UPF0289 protein yacF ORF00213 425 & 426 dephospho-CoAkinase (coaE) [2.7.1.24] ORF00214 427 & 428 guanosine monophosphatereductase (guaC) [1.7.1.7] ORF00215 429 & 430 Protein transport proteinhofC (MTB) ORF00216 431 & 432 Protein transport protein hofB (MTB)ORF00217 433 & 434 Prepilin peptidase dependent protein D precursorORF00218 435 & 436 nicotinate-nucleotide pyrophosphorylase (nadC)[2.4.2.19] ORF00219 437 & 438 AmpD protein (ampD) ORF00220 439 & 440regulates ampC ORF00221 441 & 442 Aromatic amino acid transport proteinaroP (General aromatic aminoacid permease) ORF00222 443 & 444uropathogenic specific protein ORF00223 445 & 446 unnamed proteinproduct; 9 bp deletion at the 3′ end OrfU2 ORF00224 447 & 448uropathogenic specific protein (partial) [3.1.—.—] ORF00225 449 & 450unnamed protein product; OrfU3 ORF00226 451 & 452 unnamed proteinproduct; OrfU1 ORF00227 453 & 454 Pyruvate dehydrogenase complexrepressor ORF00228 455 & 456 Pyruvate dehydrogenase E1 component (aceE)[1.2.4.1] ORF00229 457 & 458 pyruvate dehydrogenase complexdihydrolipoamide acetyltransferase (aceF) [2.3.1.12] ORF00230 459 & 460dihydrolipoamide dehydrogenase (lpdA) [1.8.1.4] ORF00231 461 & 462conserved hypothetical protein ORF00232 463 & 464 hypothetical proteinORF00233 465 & 466 hypothetical protein ORF00234 467 & 468 aconitatehydratase 2 (acnB) [4.2.1.3] ORF00235 469 & 470 S-adenosylmethioninedecarboxylase proenzyme ORF00236 471 & 472 spermidine synthase (speE)[2.5.1.16] ORF00237 473 & 474 yacC ORF00238 475 & 476 Blue copperoxidase cueO precursor ORF00239 477 & 478 Glucose dehydrogenase ORF00240479 & 480 hypoxanthine phosphoribosyltransferase (hpt) [2.4.2.8]ORF00241 481 & 482 Protein yadF [4.2.1.1] ORF00242 483 & 484 ABCtransporter ATP-binding protein ORF00243 485 & 486 ABC transporterintegral membrane protein STY0195 (membrane) ORF00244 487 & 488Fructose-specific phosphotransferase system component homolog. (PTS)[2.7.1.69] ORF00245 489 & 490 Polysaccharide deacetylase domain proteinORF00246 491 & 492 aspartate 1-decarboxylase (panD) [4.1.1.11] ORF00247493 & 494 transposase ORF00248 495 & 496 pantoate--beta-alanine ligase(panC) [6.3.2.1] ORF00249 497 & 498 3-methyl-2-oxobutanoatehydroxymethyltransferase (panB) [2.1.2.11] ORF00250 499 & 500Hypothetical fimbrial-like protein yadC precursor ORF00251 501 & 502Protein yadK ORF00252 503 & 504 Fimbrial protein subfamily ORF00253 505& 506 hypothetical protein ORF00254 507 & 508 Outer membrane usherprotein htrE precursor ORF00255 509 & 510 Chaperone protein ecpDprecursor ORF00256 511 & 512 pilin chaperone ecpD2 ORF00257 513 & 5142-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase(folK) [2.7.6.3] ORF00258 515 & 516 Poly(A) polymerase (PAP) (Plasmidcopy number protein) (PAP) [2.7.7.19] ORF00259 517 & 518 poly(A)polymerase (PAP) ORF00260 519 & 520 glutamate-tRNA ligase homolog yadBORF00261 521 & 522 dnaK suppressor protein ORF00262 523 & 524 sugarfermentation stimulation protein (sfsA) ORF00263 525 & 526 2-5 RNAligase ORF00264 527 & 528 ATP-dependent helicase HrpB (hrpB) ORF00265529 & 530 peptidoglycan synthetase penicillin-binding protein 1BORF00266 531 & 532 penicillin-binding protein 1B (mrcB) ORF00267 533 &534 Ferrichrome-iron receptor precursor (Ferric hydroxamateuptake)(Ferric hydroxamate receptor) ORF00268 535 & 536 Ferrichrometransport ATP-binding protein fhuC ORF00269 537 & 538Ferrichrome-binding periplasmic protein precursor ORF00270 539 & 540Ferrichrome transport system permease protein fhuB (membrane) ORF00271541 & 542 glutamate-1-semialdehyde-2,1-aminomutase (hemL) [5.4.3.8]ORF00272 543 & 544 H(+)—Cl(−) exchange transporter clcA ORF00273 545 &546 HesB family protein ORF00274 547 & 548 predicted membrane proteinORF00275 549 & 550 Vitamin B12 transport protein btuF precursor (PBP)ORF00276 551 & 552 MTA-SAH nucleosidase ORF00277 553 & 554deoxyguanosinetriphosphate triphosphohydrolase, putative subfamily[3.1.5.1] ORF00278 555 & 556 htrA product [3.4.21.—] ORF00279 557 & 558Carbohydrate diacid regulator ORF00280 559 & 560 Hypothetical UPF0325protein yaeH ORF00281 561 & 5622,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase(dapD) [2.3.1.117] ORF00282 563 & 564 protein-P-II uridylyltransferase(glnD) [2.7.7.59] ORF00283 565 & 566 methionine aminopeptidase, type I(map) [3.4.11.18] ORF00284 567 & 568 conserved hypothetical proteinORF00285 569 & 570 30S ribosomal protein S2 ORF00286 571 & 572translation elongation factor Ts (tsf) ORF00287 573 & 574 uridylatekinase (pyrH) [2.7.4.—] ORF00288 575 & 576 ribosome recycling factor(frr) ORF00289 577 & 578 1-deoxy-D-xylulose 5-phosphate reductoisomerase(dxr) [1.1.1.267] ORF00290 579 & 580 undecaprenyl diphosphate synthase(uppS) [2.5.1.31] ORF00291 581 & 582 phosphatidate cytidylyltransferase(cdsA) [2.7.7.41] ORF00292 583 & 584 membrane-associated zincmetalloprotease, putative ORF00293 585 & 586 outer membrane proteinprecursor yaeT (D15) ORF00294 587 & 588 histone-like protein, located inouter membrane or ORF00295 589 & 590 UDP-3-O-[3-hydroxymyristoyl]glucosamine N-acyltransferase (lpxD) [2.3.1.—] ORF00296 591 & 592(3R)-hydroxymyristol acyl carrier protein dehydratase [4.2.1.—] ORF00297593 & 594 acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamineO-acyltransferase (lpxA) [2.3.1.129] ORF00298 595 & 596lipid-A-disaccharide synthase (lpxB) [2.4.1.182] ORF00299 597 & 598ribonuclease HII (rnhB) [3.1.26.4] ORF00300 599 & 600 DNA polymerase IIIalpha subunit (dnaE) [2.7.7.7] ORF00301 601 & 602 acetyl-CoAcarboxylase, carboxyl transferase, alpha subunit (accA) [6.4.1.2]ORF00302 603 & 604 Lysine decarboxylase, constitutive (ldc) [4.1.1.18]ORF00303 605 & 606 Lysine decarboxylase, constitutive (ldc) [4.1.1.18]ORF00304 607 & 608 lactoylglutathione lyase ORF00305 609 & 610 cellcycle protein (Ile) ORF00306 611 & 612 Rof protein ORF00307 613 & 614putative cytoplasmic protein ORF00308 615 & 616 YaeQ protein ORF00309617 & 618 Peptidyl-tRNA hydrolase domain, putative ORF00310 619 & 620Copper homeostasis protein cutF precursor (lipoprotein) ORF00311 621 &622 yaeF protein ORF00312 623 & 624 prolyl-tRNA synthetase (proS)[6.1.1.15] ORF00313 625 & 626 UPF0066 protein rcsF(orf3) ORF00314 627 &628 Protein rcsF ORF00315 629 & 630 D-methionine-binding lipoproteinmetQ precursor (hlpA) ORF00316 631 & 632 D-methionine transport systempermease protein metI (membrane) ORF00317 633 & 634 D-methionine ABCtransporter, ATP-binding protein (metN) ORF00318 635 & 636 unnamedprotein product ORF00319 637 & 638 hypothetical protein ORF00320 639 &640 2,5-diketo-D-gluconic acid reductase B (orf267) [1.1.1.274] ORF00321641 & 642 unnamed protein product; Highly similar to LysR-familytranscriptional regulator, YafC protein of Escherichia coli (orf304)ORF00322 643 & 644 unnamed protein product ORF00323 645 & 646methlytransferase, UbiE-COQ5 family ORF00324 647 & 648 Membrane-boundlytic murein transglycosylase D precursor(Murein hydrolase D)(Regulatory protein dniR) [3.2.1.—] ORF00325 649 & 650hydroxyacylglutathione hydrolase [3.1.2.6] ORF00326 651 & 652hypothetical protein ORF00327 653 & 654 conserved hypothetical proteinORF00328 655 & 656 RNase Hl, degrades RNA of DNA-RNA hybrids ORF00329657 & 658 DNA polymerase III, epsilon subunit (dnaQ) [2.7.7.7] ORF00330659 & 660 Hypothetical lipoprotein yafT precursor ORF00331 661 & 662Aec32 ORF00332 663 & 664 Aec31 ORF00333 665 & 666 Aec30 ORF00334 667 &668 Aec29 ORF00335 669 & 670 hypothetical protein ORF00336 671 & 672Aec28 ORF00337 673 & 674 Aec27 ORF00338 675 & 676 Aec26 ORF00339 677 &678 Aec25 ORF00340 679 & 680 Aec24 ORF00341 681 & 682 Aec23 ORF00342 683& 684 hypothetical protein ORF00343 685 & 686 Aec22 ORF00344 687 & 688Aec20 ORF00345 689 & 690 Aec19 ORF00346 691 & 692 Aec18 ORF00347 693 &694 Aec17 ORF00348 695 & 696 Aec16 ORF00349 697 & 698 Aec15 (AF044503)ORF00350 699 & 700 Aec14 ORF00351 701 & 702 Aec13 ORF00352 703 & 704Transposase (IS4 family) ORF00353 705 & 706 Aec7 ORF00354 707 & 708possible hydrolase ORF00355 709 & 710 hypothetical protein ORF00356 711& 712 Inhibitor of vertebrate lysozyme precursor ORF00357 713 & 714Acyl-CoA dehydrogenase (EC 1.3.99.—). [1.3.99.—] ORF00358 715 & 716Phosphoheptose isomerase (gmhA) [5.—.—.—] ORF00359 717 & 718 Bacterialprotein of unknown function (DUF949) superfamily ORF00360 719 & 720Predicted glutamine amidotransferase ORF00361 721 & 722 conservedhypothetical protein ORF00362 723 & 724 Hypothetical lipoprotein yafLprecursor ORF00363 725 & 726 Protein of unknown function (DUF1568)domain protein ORF00364 727 & 728 FhiA protein ORF00365 729 & 730 MbhAprotein ORF00366 731 & 732 DNA polymerase IV (Pol IV) [2.7.7.7] ORF00367733 & 734 prevent-host-death family protein ORF00368 735 & 736 unnamedprotein product ORF00369 737 & 738 Hypothetical acetyltransferase yafP[2.3.1.—] ORF00370 739 & 740 uncharacterized conserved protein,RtcB-UPF0027 family CAC3383 ORF00371 741 & 742 Peptide chain releasefactor homolog (RF) ORF00372 743 & 744 aminoacyl-histidine dipeptidasePepD ORF00373 745 & 746 Xanthine-guanine phosphoribosyltransferase(XGPRT) [2.4.2.22] ORF00374 747 & 748 Hydrolases of the alpha-betasuperfamily ORF00375 749 & 750 Curlin genes transcriptional activatoryprotein ORF00376 751 & 752 Outer membrane pore protein E precursorORF00377 753 & 754 glutamate 5-kinase (proB) [2.7.2.11] ORF00378 755 &756 gamma-glutamyl phosphate reductase (proA) [1.2.1.41] ORF00379 757 &758 HTH-type transcriptional regulator prsX ORF00380 759 & 760Haemoglobin protease ORF00381 761 & 762 InsA ORF00382 763 & 764 InsBORF00383 765 & 766 conserved hypothetical protein ORF00384 767 & 768YagU ORF00385 769 & 770 Uncharacterised protein family (UPF0153)superfamily ORF00386 771 & 772 conserved hypothetical protein ORF00387773 & 774 conserved hypothetical protein ORF00388 775 & 776 conservedhypothetical protein ORF00389 777 & 778 conserved hypothetical proteinORF00390 779 & 780 MatB precursor ORF00391 781 & 782 transcriptionalregulator, LuxR family domain protein ORF00392 783 & 784 ribosomalprotein L36 (rpmJ) ORF00393 785 & 786 tail protein ORF00394 787 & 788Sb24 ORF00395 789 & 790 tail fiber assembly protein ORF00396 791 & 792T4-exclusion protein gpIBEGd ORF00397 793 & 794 hypothetical proteinORF00398 795 & 796 hypothetical protein ORF00399 797 & 798NADH-dependent flavin oxidoreductase, Oye family [1.—.—.—] ORF00400 799& 800 dienelactone hydrolase domain protein (AE000230) ORF00401 801 &802 transcription regulator, LysR family Atu5241 ORF00402 803 & 804transcriptional regulator (LysR family) ORF00403 805 & 806oxidoreductase, aldo-keto reductase family ORF00404 807 & 8082,5-diketo-D-gluconic acid reductase A [1.1.1.—] ORF00405 809 & 810Putative adhesin ORF00406 811 & 812 Hypothetical transcriptionalregulator ykgA ORF00407 813 & 814 2,5-diketo-D-gluconic acid reductase AORF00408 815 & 816 MC21 ORF00409 817 & 818 Protein of unknown function(DUF1471) superfamily ORF00410 819 & 820 pyridine nucleotide-disulfideoxidoreductase [1.16.1.1] ORF00411 821 & 822 Hypotheticaltranscriptional regulator ykgD ORF00412 823 & 824 Cysteine-rich domainprotein ORF00413 825 & 826 iron-sulfur cluster binding protein ORF00414827 & 828 Uncharacterized ACR, YkgG family COG1556 family ORF00415 829 &830 hypothetical protein ORF00416 831 & 832 outer membraneautotransporter barrel domain protein ORF00417 833 & 834 conservedhypothetical protein ORF00418 835 & 836 conserved hypothetical proteinORF00419 837 & 838 Type 1 fimbriae Regulatory protein fimB ORF00420 839& 840 choline dehydrogenase (betA) [1.1.99.1] ORF00421 841 & 842 betainealdehyde dehydrogenase (betB) [1.2.1.8] ORF00422 843 & 844 Regulatoryprotein betI (U73857) ORF00423 845 & 846 High-affinity choline transportprotein ORF00424 847 & 848 EAL domain protein ORF00425 849 & 850 allSORF00426 851 & 852 glutathione-regulated potassium-efflux system proteinORF00427 853 & 854 ankyrin repeat domain protein ORF00428 855 & 856conserved hypothetical protein ORF00429 857 & 858 YahF-FdrA-like proteinORF00430 859 & 860 YahG-YlbE-like protein ORF00431 861 & 862 Carbamatekinase-like protein yahI [2.7.2.2] ORF00432 863 & 864 Cytosine deaminaseORF00433 865 & 866 Protein of unknown function (DUF984) superfamilyORF00434 867 & 868 ABC transporter, substrate binding protein [sugar](AE005211) ORF00435 869 & 870 ribose ABC transporter (ATP-bindingprotein) ORF00436 871 & 872 ribose ABC transporter, permease protein,putative ORF00437 873 & 874 ABC sugar transporter, permease subunit(permease) ORF00438 875 & 876 oxidoreductase, zinc-binding [1.1.1.2]ORF00439 877 & 878 RhtC-like transporter STY0397 (RhtC) ORF00440 879 &880 Protein of unknown function (DUF1471) superfamily ORF00441 881 & 882propionate catabolism operon regulatory protein PrpR (prpR) ORF00442 883& 884 hypothetical protein ORF00443 885 & 886 regulator for prp operonORF00444 887 & 888 hypothetical protein ORF00445 889 & 890methylisocitrate lyase (prpB) [4.1.3.30] ORF00446 891 & 8922-methylcitrate synthase (Methylcitrate synthase)(Citrate synthase 2)[2.3.3.5] ORF00447 893 & 894 2-methylcitrate dehydratase (prpD)[4.2.1.79] ORF00448 895 & 896 propionate--CoA ligase (prpE) [6.2.1.17]ORF00449 897 & 898 cytosine transporter, putative ORF00450 899 & 900Cytosine deaminase ORF00451 901 & 902 Galactoside O-acetyltransferase[2.3.1.18] ORF00452 903 & 904 Lactose permease (Lactose-proton symport)ORF00453 905 & 906 Beta-galactosidase (lacZ) [3.2.1.23] ORF00454 907 &908 transcriptional regulator, lacI family domain protein, putativeORF00455 909 & 910 Arac-family transcriptional regulator ORF00456 911 &912 nucleoprotein-polynucleotide-associated enzyme ORF00457 913 & 914carboxylesterase [3.1.1.1] ORF00458 915 & 916 alcohol dehydrogenase[similarity] (adhC) [1.—.—.—] ORF00459 917 & 918 putative alpha helixchain ORF00460 919 & 920 conserved hypothetical protein ORF00461 921 &922 Sugar phosphate nucleotydyl transferase ORF00462 923 & 924glucosaminyltransferase ORF00463 925 & 926 Putative conserved proteinORF00464 927 & 928 taurine ABC transporter, periplasmic binding protein(tauA) ORF00465 929 & 930 taurine ABC transporter, ATP-binding protein(cmpC) ORF00466 931 & 932 Taurine transport system permease protein tauCORF00467 933 & 934 alpha-ketoglutarate-dependent taurine dioxygenase[1.—.—.—] ORF00468 935 & 936 delta-aminolevulinic acid dehydratase(hemB) [4.2.1.24] ORF00469 937 & 938 puative autotransporter-virulencefactor STY0405 (partial) ORF00470 939 & 940 conserved hypotheticalprotein ORF00471 941 & 942 beta-lactamase ORF00472 943 & 944 SbmAprotein ORF00473 945 & 946 lipoprotein, putative ORF00474 947 & 948conserved hypothetical protein ORF00475 949 & 950 conserved hypotheticalprotein ORF00476 951 & 952 D-alanine--D-alanine ligase A(D-alanylalanine synthetaseA) (D-Ala-D-Ala ligase A) [6.3.2.4] ORF00477953 & 954 conserved hypothetical protein ORF00478 955 & 956 LD01705pORF00479 957 & 958 Alkaline phosphatase precursor ORF00480 959 & 960induced by phosphate starvation ORF00481 961 & 962 AdrA protein (GGDEF)ORF00482 963 & 964 pyrroline-5-carboxylate reductase (proC) [1.5.1.2]ORF00483 965 & 966 YaiI-YqxD family protein family ORF00484 967 & 968shikimate kinase (aroK) [2.7.1.71] ORF00485 969 & 970 conservedhypothetical protein ORF00486 971 & 972 AroM protein ORF00487 973 & 974uncharacterized protein conserved in bacteria ORF00488 975 & 976conserved hypothetical protein ORF00489 977 & 978 conserved hypotheticalprotein ORF00490 979 & 980 Recombination associated protein rdgCORF00491 981 & 982 possible NAGC-like transcriptional regulator ORF00492983 & 984 major facilitator family transporter ORF00493 985 & 986Exonuclease sbcC (P-loop) [3.1.15.—] ORF00494 987 & 988 exonuclease SbcD(SbcD) [3.1.—.—] ORF00495 989 & 990 conserved hypothetical proteinORF00496 991 & 992 phosphate regulon transcriptional regulatory proteinPhoB (phoB) ORF00497 993 & 994 Phosphate regulon sensor protein phoRORF00498 995 & 996 hypothetical protein ORF00499 997 & 998 conservedhypothetical protein ORF00500 999 & 1000 branched-chain amino acidtransport system II carrier protein (brnQ) ORF00501 1001 & 1002Proline-specific permease proY ORF00502 1003 & 1004 maltodextringlucosidase [3.2.1.20] ORF00503 1005 & 1006 Protein of unknown function,DUF479 superfamily ORF00504 1007 & 1008 S-adenosylmethionine: tRNAribosyltransferase-isomerase (queA) [5.—.—.—] ORF00505 1009 & 1010queuine tRNA-ribosyltransferase (tgt) [2.4.2.29] ORF00506 1011 & 1012preprotein translocase, YajC subunit, putative ORF00507 1013 & 1014Protein-export membrane protein secD ORF00508 1015 & 1016 protein-exportmembrane protein secF ORF00509 1017 & 1018 HNH endonuclease domainprotein ORF00510 1019 & 1020 Nucleoside-specific channel-forming proteintsx precursor ORF00511 1021 & 1022 yajI protein ORF00512 1023 & 1024conserved hypothetical protein TIGR00244 ORF00513 1025 & 1026 riboflavinbiosynthesis protein RibD (ribD) [3.5.4.26 1.1.1.193] ORF00514 1027 &1028 6,7-dimethyl-8-ribityllumazine synthase ORF00515 1029 & 1030transcription antitermination factor NusB (nusB) ORF00516 1031 & 1032thiamine-monophosphate kinase (thiL) [2.7.4.16] ORF00517 1033 & 1034Phosphatidylglycerophosphatase A ORF00518 1035 & 1036 probableoxidoreductase yajO [1.—.—.—] ORF00519 1037 & 10381-deoxy-D-xylulose-5-phosphate synthase (dxs) [2.2.1.7] ORF00520 1039 &1040 Geranyltranstransferase (Farnesyl-diphosphate synthase)(FPPsynthase) (ispA) [2.5.1.10] ORF00521 1041 & 1042 exodeoxyribonucleaseVII, small subunit (xseB) [3.1.11.6] ORF00522 1043 & 1044 hypotheticalprotein ORF00523 1045 & 1046 Thiamine biosynthesis protein thiI (thiI)ORF00524 1047 & 1048 4-methyl-5(B-hydroxyethyl)-thiazole monophosphatebiosynthesis enzyme (ThiJ) ORF00525 1049 & 1050 2-dehydropantoate2-reductase (panE) [1.1.1.169] ORF00526 1051 & 1052 Protein yajQORF00527 1053 & 1054 Hypothetical transport protein yajR ORF00528 1055 &1056 protoheme IX farnesyltransferase (cyoE) [2.5.1.—] ORF00529 1057 &1058 cytochrome o ubiquinol oxidase C subunit [1.10.3.—] ORF00530 1059 &1060 Cytochrome c oxidase subunit III ORF00531 1061 & 1062 Ubiquinoloxidase polypeptide I (Cytochrome o subunit 1)(Oxidase BO(3) subunit 1)(Cytochrome o ubiquinol oxidase subunit 1)(Ubiquinol oxidase chain A)[1.10.3.—] ORF00532 1063 & 1064 Ubiquinol oxidase polypeptide IIprecursor (Cytochrome osubunit 2) (Oxidase BO(3) subunit 2) (Cytochromeo ubiquinol oxidasesubunit 2) (Ubiquinol oxidase chain B) [1.10.3.—]ORF00533 1065 & 1066 beta-lactamase induction signal transducer AmpGORF00534 1067 & 1068 AmpG-signal transducer ORF00535 1069 & 1070putative polymerase-proteinase ORF00536 1071 & 1072 BolA proteinORF00537 1073 & 1074 conserved hypothetical protein ORF00538 1075 & 1076Trigger factor (TF) (tig) ORF00539 1077 & 1078 conserved hypotheticalprotein ORF00540 1079 & 1080 trigger factor (tig) [5.2.1.8] ORF005411081 & 1082 ATP-dependent Clp protease, proteolytic subunit ClpP (clpP)[3.4.21.92] ORF00542 1083 & 1084 ATP-dependent Clp protease, ATP-bindingsubunit ClpX (clpX) ORF00543 1085 & 1086 ATP-dependent protease La (Ion)[3.4.21.53] ORF00544 1087 & 1088 DNA-binding protein HU-beta, NS1 (HU)ORF00545 1089 & 1090 Peptidyl-prolyl cis-trans isomerase D (PPlaseD)(Rotamase D) [5.2.1.8] ORF00546 1091 & 1092 DNA uptake proteinORF00547 1093 & 1094 Predicted thioesterase ORF00548 1095 & 1096 exsBprotein ORF00549 1097 & 1098 conserved hypothetical protein ORF005501099 & 1100 Cof protein ORF00551 1101 & 1102 transcriptional regulator,AsnC family ORF00552 1103 & 1104 Multidrug resistance-like ATP-bindingprotein mdlA (atp) ORF00553 1105 & 1106 multidrug resistance-likeATP-binding protein Mdl ORF00554 1107 & 1108 glutamine synthetaseactivity regulation protein ORF00555 1109 & 1110 ammonium transporter(amt) ORF00556 1111 & 1112 acyl-CoA thioesterase II (tesB) [3.1.2.—]ORF00557 1113 & 1114 hypothetical protein ORF00558 1115 & 1116glycoprotein-polysaccharide metabolism ORF00559 1117 & 1118 predictedmethylated DNA-protein cysteine methyltransferase ORF00560 1119 & 1120Protein of unknown function (DUF1428) superfamily ORF00561 1121 & 1122EAL domain protein ORF00562 1123 & 1124 conserved hypothetical proteinORF00563 1125 & 1126 Maltose O-acetyltransferase [2.3.1.79] ORF005641127 & 1128 hypothetical protein ORF00565 1129 & 1130 Hha proteinORF00566 1131 & 1132 YmoB ORF00567 1133 & 1134 Acriflavine resistanceprotein B ORF00568 1135 & 1136 Acriflavine resistance protein Aprecursor ORF00569 1137 & 1138 HTH-type transcriptional regulator acrR(Potential acrAB operonrepressor) (acrEF) ORF00570 1139 & 1140 Potassiumefflux system kefA ORF00571 1141 & 1142 conserved hypothetical proteinORF00572 1143 & 1144 primosomal replication protein N ORF00573 1145 &1146 Protein of unknown function (DUF454) family ORF00574 1147 & 1148hypothetical protein ORF00575 1149 & 1150 adeninephosphoribosyltransferase (apt) [2.4.2.7] ORF00576 1151 & 1152 DNApolymerase III, subunits gamma and tau, programmed (dnaX) [2.7.7.7]ORF00577 1153 & 1154 conserved hypothetical protein TIGR00103 ORF005781155 & 1156 recombination protein RecR (recR) ORF00579 1157 & 1158Chaperone protein htpG (Heat shock protein htpG) (Hightemperatureprotein G) (Heat shock protein C62.5) (htpG) ORF00580 1159 &1160 Adenylate kinase ORF00581 1161 & 1162 ferrochelatase (hemH)[4.99.1.1] ORF00582 1163 & 1164 Acetyl esterase [3.1.1.—] ORF00583 1165& 1166 Inosine-guanosine kinase [2.7.1.73] ORF00584 1167 & 1168 RosBORF00585 1169 & 1170 fosmidomycin resistance protein (CAMPs) ORF005861171 & 1172 Protein ushA precursor [Includes: UDP-sugarhydrolase(UDP-sugar diphosphatase) (UDP-sugar pyrophosphatase);5′-nucleotidase(EC 3.1.3.5) (5′-NT)] (5-NT) [3.6.1.45] ORF00587 1173 &1174 hypothetical protein ORF00588 1175 & 1176 hypothetical proteinORF00589 1177 & 1178 ybaK-ebsC protein (ybaK) ORF00590 1179 & 1180 GumNprotein superfamily ORF00591 1181 & 1182 probable killer protein VCA0391ORF00592 1183 & 1184 unnamed protein product; Some similarities withvirulence associated protein A (vapA) ORF00593 1185 & 1186Copper-transporting P-type ATPase [3.6.1.—] ORF00594 1187 & 1188K-3-type glutaminase [3.5.1.2] ORF00595 1189 & 1190 amino acid permeasefamily protein, putative ORF00596 1191 & 1192 Cu(I)-responsivetranscriptional regulator (cueR) ORF00597 1193 & 1194 hypotheticalprotein ORF00598 1195 & 1196 lipoprotein, putative ORF00599 1197 & 1198adhesin-invasin-like protein ORF00600 1199 & 1200 conserved hypotheticalprotein ORF00601 1201 & 1202 membrane protein ORF00602 1203 & 1204 band7-Mec-2 family protein ORF00603 1205 & 1206 ABC transporter, ATP-bindingprotein ORF00604 1207 & 1208 membrane protein, putative ORF00605 1209 &1210 ybbN protein ORF00606 1211 & 1212 oxidoreductase, short-chaindehydrogenase-reductase family (SDR) [1.—.—.—] ORF00607 1213 & 1214acyl-coA thioesterase I precursor [3.1.2.—] ORF00608 1215 & 1216 ABCtransporter, ATP-binding protein ORF00609 1217 & 1218 permease, putativedomain protein ORF00610 1219 & 1220 YbbB ORF00611 1221 & 1222 allSORF00612 1223 & 1224 Ureidoglycolate hydrolase (allA) [3.5.3.19]ORF00613 1225 & 1226 Negative regulator of allantoin and glyoxylateutilization operons(Transcriptional regulator allR) ORF00614 1227 & 1228glyoxylate carboligase (gcl) [4.1.1.47] ORF00615 1229 & 1230Hydroxypyruvate isomerase [5.3.1.22] ORF00616 1231 & 12322-hydroxy-3-oxopropionate reductase [1.1.1.60] ORF00617 1233 & 1234cytosine-purine-uracil-thiamine-allantoin permease family protein,putative ORF00618 1235 & 1236 Allantoinase [3.5.2.5] ORF00619 1237 &1238 Putative purine permease ybbY ORF00620 1239 & 1240 Glycerate kinase1 ORF00621 1241 & 1242 GlxB6 ORF00622 1243 & 1244 Allantoateamidohydrolase (partial) ORF00623 1245 & 1246 Ureidoglycolatedehydrogenase [1.1.1.154] ORF00624 1247 & 1248 Protein fdrA ORF006251249 & 1250 conserved hypothetical protein ORF00626 1251 & 1252 Proteinof unknown function (DUF1116) superfamily ORF00627 1253 & 1254 conservedhypothetical protein ORF00628 1255 & 1256 carbamate kinase (arcC)[2.7.2.2] ORF00629 1257 & 1258 phosphoribosylaminoimidazole carboxylase,ATPase subunit (purK) [4.1.1.21] ORF00630 1259 & 1260phosphoribosylaminoimidazole carboxylase, catalytic subunit (purE)[4.1.1.21] ORF00631 1261 & 1262 conserved hypothetical protein ORF006321263 & 1264 UDP-2,3-diacylglucosamine hydrolase (lpxH) [3.6.1.—]ORF00633 1265 & 1266 peptidyl-prolyl cis-trans isomerase B (ppiB)[5.2.1.8] ORF00634 1267 & 1268 cysteinyl-tRNA synthetase (cysS)[6.1.1.16] ORF00635 1269 & 1270 Predicted membrane-bound metal-dependenthydrolase (DUF457) family ORF00636 1271 & 1272 UncharacterizedACR-related protein ORF00637 1273 & 1274 5,10-methylene-tetrahydrofolatedehydrogenase (folD) [1.5.1.5] ORF00638 1275 & 1276 Prophage DLP12integrase (Prophage QSR′ integrase) ORF00639 1277 & 1278 conservedhypothetical protein ORF00640 1279 & 1280 Gp37 ORF00641 1281 & 1282conserved hypothetical protein ORF00642 1283 & 1284 Sb36 ORF00643 1285 &1286 conserved hypothetical protein ORF00644 1287 & 1288 unknown proteinencoded by prophage CP-933N ORF00645 1289 & 1290 antitermination proteinQ ORF00646 1291 & 1292 unknown protein encoded by prophage CP-933OORF00647 1293 & 1294 DNA adenine-methylase ORF00648 1295 & 1296 lysisprotein ORF00649 1297 & 1298 conserved hypothetical protein ORF006501299 & 1300 Protein of unknown function (DUF1327) superfamily ORF006511301 & 1302 Lysozyme (Lysis protein) (Muramidase) (Endolysin) [3.2.1.17]ORF00652 1303 & 1304 lipoprotein Rz1 precursor -related protein ORF006531305 & 1306 Bacteriophage lysis protein ORF00654 1307 & 1308 Bor proteinhomolog from lambdoid prophage DLP12 ORF00655 1309 & 1310 hypotheticalprotein ORF00656 1311 & 1312 Partial tonB-like membrane protein encodedwithin prophage ORF00657 1313 & 1314 conserved hypothetical proteinORF00658 1315 & 1316 Terminase small subunit (DNA packaging protein NU1)(fragment) ORF00659 1317 & 1318 Phage terminase large subunit (GpA)ORF00660 1319 & 1320 gpW ORF00661 1321 & 1322 phage portal protein,lambda family ORF00662 1323 & 1324 head-tail preconnector protein GP5ORF00663 1325 & 1326 Bacteriophage lambda head decoration protein DORF00664 1327 & 1328 Phage major capsid protein E ORF00665 1329 & 1330DNA packaging protein FI ORF00666 1331 & 1332 Phage Head-Tail AttachmentORF00667 1333 & 1334 Prophage minor tail protein Z (GPZ) ORF00668 1335 &1336 Phage minor tail protein U ORF00669 1337 & 1338 Major tail proteinV ORF00670 1339 & 1340 phage minor tail protein G ORF00671 1341 & 1342minor tail protein ORF00672 1343 & 1344 tail length tape measure proteinprecursor ORF00673 1345 & 1346 phage tail tape measure protein, lambdafamily ORF00674 1347 & 1348 phage minor tail protein L ORF00675 1349 &1350 Putative tail fiber component K of prophage ORF00676 1351 & 1352Bacteriophage lambda tail assembly protein I ORF00677 1353 & 1354 hostspecificity protein (partial) ORF00678 1355 & 1356 host specificityprotein (partial) ORF00679 1357 & 1358 Lom ORF00680 1359 & 1360conserved hypothetical protein ORF00681 1361 & 1362 conservedhypothetical protein ORF00682 1363 & 1364 conserved hypothetical proteinORF00683 1365 & 1366 conserved hypothetical protein ORF00684 1367 & 1368Tail fiber assembly protein homolog from lambdoid prophage ORF00685 1369& 1370 proteinase, putative ORF00686 1371 & 1372 Protease VII precursor(Omptin) (Outer membrane protein3B) (Protease A) (SopA) [3.4.21.87]ORF00687 1373 & 1374 Prophage DLP12 integrase (Prophage QSR′ integrase)ORF00688 1375 & 1376 Tail fiber assembly protein homolog from lambdoidORF00689 1377 & 1378 Protease VII precursor (Omptin) (Outer membraneprotein3B) (Protease A) (SopA) [3.4.21.87] ORF00690 1379 & 1380conserved hypothetical protein ORF00691 1381 & 1382 Bacteriophage N4adsorption protein A precursor ORF00692 1383 & 1384 bacteriophage N4adsorption protein NfrB ORF00693 1385 & 1386 hypothetical proteinORF00694 1387 & 1388 Sensor kinase cusS [2.7.3.—] ORF00695 1389 & 1390Transcriptional regulatory protein cusR ORF00696 1391 & 1392 Cationefflux system protein cusC precursor ORF00697 1393 & 1394 Cation effluxsystem protein cusF precursor ORF00698 1395 & 1396 Cation efflux systemprotein cusB precursor ORF00699 1397 & 1398 Cation efflux system proteincusA (CzcA) ORF00700 1399 & 1400 Phenylalanine-specific permeaseORF00701 1401 & 1402 putative transport ORF00702 1403 & 1404Oxygen-insensitive NAD(P)H nitroreductase [1.—.—.—] ORF00703 1405 & 1406Uncharacterized protein conserved in bacteria ORF00704 1407 & 1408Protein of unknown function (DUF1158) superfamily ORF00705 1409 & 1410Glutamate-cysteine ligase family 2, putative ORF00706 1411 & 14124-phosphopantetheinyl transferase entD ORF00707 1413 & 1414ferrienterobactin receptor precursor ORF00708 1415 & 1416 conservedhypothetical protein ORF00709 1417 & 1418 Enterochelin esterase ORF007101419 & 1420 mbtH protein-related protein ORF00711 1421 & 1422enterobactin synthetase component F [2.7.7.—] ORF00712 1423 & 1424Ferric enterobactin transport protein fepE (enterochelin) ORF00713 1425& 1426 ferric exochelin uptake (fxuB) ORF00714 1427 & 1428 ferricexochelin uptake (fxuA) ORF00715 1429 & 1430 ferric exochelin uptake(fxuC) ORF00716 1431 & 1432 membrane protein, putative ORF00717 1433 &1434 ferric enterobactin (enterochelin) binding protein; periplasmiccomponent ORF00718 1435 & 1436 isochorismate synthase [5.4.99.6]ORF00719 1437 & 1438 2,3-dihydroxybenzoate-AMP ligase [2.7.7.58]ORF00720 1439 & 1440 Isochorismatase (Isochorismate lyase) (2,3dihydro-2,3dihydroxybenzoate synthase) (Enterobactin synthetasecomponent B)(Enterochelin synthase B) (EntB) [3.3.2.1] ORF00721 1441 &1442 oxidoreductase, short-chain dehydrogenase-reductase family[1.3.1.28] ORF00722 1443 & 1444 Hypothetical UPF0152 protein ybdB (p15)ORF00723 1445 & 1446 Carbon starvation protein A ORF00724 1447 & 1448Uncharacterized small protein ORF00725 1449 & 1450 glyceroldehydrogenase [1.1.—.—] ORF00726 1451 & 1452 aminotransferase, class I[2.6.1.—] ORF00727 1453 & 1454 unnamed protein product; ORF_ID: o166#4ORF00728 1455 & 1456 conserved hypothetical protein ORF00729 1457 & 1458Hypothetical transcriptional regulator ybdO (orf2) ORF00730 1459 & 1460Thiol: disulfide interchange protein dsbG precursor ORF00731 1461 & 1462alkyl hydroperoxide reductase c22 protein [1.6.4.—] ORF00732 1463 & 1464alkyl hydroperoxide reductase F52A protein [1.6.4.—] ORF00733 1465 &1466 Universal stress protein G ORF00734 1467 & 1468 Regulator ofnucleoside diphosphate kinase ORF00735 1469 & 1470 Ribonuclease Iprecursor [3.1.27.6] ORF00736 1471 & 1472 Citrate carrier-transporter(antiport) ORF00737 1473 & 1474 triphosphoribosyl-dephospho-CoA synthase(citG) [2.7.8.25] ORF00738 1475 & 1476 Apo-citrate lyasephosphoribosyl-dephospho-CoA transferase (citX) [2.7.7.—] ORF00739 1477& 1478 citrate lyase, alpha subunit (citF) [2.8.3.10] ORF00740 1479 &1480 citrate lyase, beta subunit (citE) [4.1.3.6] ORF00741 1481 & 1482citrate lyase acyl carrier protein (citD) ORF00742 1483 & 1484 citratelyase ligase (citC) [6.2.1.22] ORF00743 1485 & 1486 Sensor kinase dpiBORF00744 1487 & 1488 Transcriptional regulatory protein dpiA(Destabilizer of plasmidinheritance) ORF00745 1489 & 1490 transport ofdicarboxylates ORF00746 1491 & 1492 CrcA protein ORF00747 1493 & 1494Cold shock-like protein cspE ORF00748 1495 & 1496 crcB protein (crcB)ORF00749 1497 & 1498 nitrilase ORF00750 1499 & 1500 Sec-independentprotein translocase protein tatA (YIGT) ORF00751 1501 & 1502 lipoic acidsynthetase (lipA) ORF00752 1503 & 1504 lipoate-protein ligase B (lipB)ORF00753 1505 & 1506 UPF0250 protein ORF00754 1507 & 1508penicillin-binding protein 5 (PBP) [3.4.16.4] ORF00755 1509 & 1510 Rarelipoprotein A precursor (rlpA) ORF00756 1511 & 1512 conservedhypothetical protein ORF00757 1513 & 1514 rod shape-determining proteinRodA (rodA) ORF00758 1515 & 1516 Penicillin-binding protein 2 (PBP-2)(PBP) ORF00759 1517 & 1518 Penicillin-binding protein 2 (PBP-2) (PBP)ORF00760 1519 & 1520 conserved hypothetical protein TIGR00246 ORF007611521 & 1522 unnamed protein product ORF00762 1523 & 1524phosphoglycerate mutase family protein, putative [3.1.3.73] ORF007631525 & 1526 nicotinate (nicotinamide) nucleotide adenylyltransferase(nadD) [2.7.7.18] ORF00764 1527 & 1528 DNA polymerase III, delta subunit(holA) [2.7.7.7] ORF00765 1529 & 1530 a minor lipoprotein ORF00766 1531& 1532 leucyl-tRNA synthetase (leuS) [6.1.1.4] ORF00767 1533 & 1534Methyl-accepting chemotaxis protein ORF00768 1535 & 1536 Inosine-uridinenucleoside N-ribohydrolase (AL355920) ORF00769 1537 & 1538Glutamate-aspartate transport ATP-binding protein gltL ORF00770 1539 &1540 Glutamate-aspartate transport system permease protein gltK(membrane) ORF00771 1541 & 1542 Glutamate-aspartate transport systempermease protein gltJ ORF00772 1543 & 1544 glutamate-aspartate ABCtransporter, periplasmic glutamate-aspartate-binding protein, truncationORF00773 1545 & 1546 Rhomboid family family ORF00774 1547 & 1548Apolipoprotein N-acyltransferase (ALP N-acyltransferase)(Copperhomeostasis protein cutE) [2.3.1.—] ORF00775 1549 & 1550 Magnesium andcobalt efflux protein corC (tlyC) ORF00776 1551 & 1552 predictedmetal-dependent hydrolase ORF00777 1553 & 1554 PhoH-like proteinORF00778 1555 & 1556 tRNA-i(6)A37 thiotransferase enzyme MiaB (miaB)ORF00779 1557 & 1558 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinolhydroxylase ORF00780 1559 & 1560 Asparagine synthetase B[glutamine-hydrolyzing] ORF00781 1561 & 1562 nagD protein, degenerateORF00782 1563 & 1564 N-acetylglucosamine repressor ORF00783 1565 & 1566N-acetylglucosamine-6-phosphate deacetylase (nagA) [3.5.1.25] ORF007841567 & 1568 glucosamine-6-phosphate isomerase (nagB) [3.5.99.6] ORF007851569 & 1570 PTS system, N-acetylglucosamine-specific IIABC component(EII-Nag) [2.7.1.69] ORF00786 1571 & 1572 membrane protein, putativeORF00787 1573 & 1574 hypothetical protein ORF00788 1575 & 1576Hypthetical protein ORF00789 1577 & 1578 Hypthetical protein ORF007901579 & 1580 TerC protein ORF00791 1581 & 1582 TerC protein ORF00792 1583& 1584 dihydrodipicolinate synthase (AE004999) [4.2.1.52] ORF00793 1585& 1586 Putative alcohol dehydrogenase (U59485) ORF00794 1587 & 1588Putative inner membrane protein ORF00795 1589 & 15904-hydroxythreonine-4-phosphate dehydrogenase (pdxA) [1.1.1.262] ORF007961591 & 1592 Transcriptional regulator of sugar metabolism, putativeORF00797 1593 & 1594 glutaminyl-tRNA synthetase (glnS) [6.1.1.18]ORF00798 1595 & 1596 hypothetical protein ORF00799 1597 & 1598 unnamedprotein product; ORF_ID: o172#6 ORF00800 1599 & 1600 Hypotheticallipoprotein ybfN precursor ORF00801 1601 & 1602 Ferric uptake regulationprotein (Ferric uptake regulator) ORF00802 1603 & 1604 Flavodoxin 1ORF00803 1605 & 1606 urea amidolyase-related protein ORF00804 1607 &1608 urea amidolyase-related protein ORF00805 1609 & 1610 SeqA proteinORF00806 1611 & 1612 phosphoglucomutase, alpha-D-glucosephosphate-specific (pgm) [5.4.2.2] ORF00807 1613 & 1614Putrescine-ornithine antiporter (Putrescine transport protein) (potE)ORF00808 1615 & 1616 Ornithine decarboxylase, inducible (speF)[4.1.1.17] ORF00809 1617 & 1618 conserved hypothetical protein ORF008101619 & 1620 hypothetical protein ORF00811 1621 & 1622 kdpE ORF00812 1623& 1624 sensor histidine kinase KdpD, putative ORF00813 1625 & 1626K+-transporting ATPase, C subunit (kdpC) [3.6.3.12] ORF00814 1627 & 1628K+-transporting ATPase, B subunit (kdpB) [3.6.3.12] ORF00815 1629 & 1630K+-transporting ATPase, A subunit (kdpA) [3.6.3.12] ORF00816 1631 & 1632K+-transporting ATPase, F subunit (kdpF) [3.6.3.12] ORF00817 1633 & 1634conserved hypothetical protein ORF00818 1635 & 1636 uncharacterizedconserved protein (orf169) ORF00819 1637 & 1638 Deoxyribodipyrimidinephotolyase [4.1.99.3] ORF00820 1639 & 1640 PTR2-family transport proteinSTY0750 ORF00821 1641 & 1642 conserved hypothetical protein TIGR00486ORF00822 1643 & 1644 conserved hypothetical protein TIGR00370 ORF008231645 & 1646 urea amidolyase-related protein ORF00824 1647 & 1648LamB-YcsF family protein (lamB) ORF00825 1649 & 1650formamidopyrimidine-DNA glycosylase, putative [3.2.—.—] ORF00826 1651 &1652 membrane protein, putative ORF00827 1653 & 1654 ybgO proteinORF00828 1655 & 1656 citrate synthase I (gltA) [2.3.3.1] ORF00829 1657 &1658 succinate dehydrogenase cytochrome b-556 subunit (AF007569)ORF00830 1659 & 1660 Succinate dehydrogenase hydrophobic membrane anchorprotein (sdhD) [1.3.99.1] ORF00831 1661 & 1662 succinate dehydrogenase,flavoprotein subunit (sdhA) ORF00832 1663 & 1664 succinatedehydrogenase, iron sulfur protein [1.3.99.1] ORF00833 1665 & 16662-oxoglutarate dehydrogenase, E1 component (sucA) [1.2.4.2] ORF008341667 & 1668 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamidesuccinyltransferase (sucB) [2.3.1.61] ORF00835 1669 & 1670Dihydrolipoamide succinyltransferase component of 2-oxoglutaratedehydrogenase complex [2.3.1.61] ORF00836 1671 & 1672 Succinyl-CoAsynthetase beta chain (SCS-beta) (sucC) [6.2.1.5] ORF00837 1673 & 1674succinyl-CoA synthetase, alpha subunit (sucD) [6.2.1.5] ORF00838 1675 &1676 uncharacterized protein, probably surface-located ORF00839 1677 &1678 hypothetical protein ORF00840 1679 & 1680 cytochrome d ubiquinoloxidase chain I [similarity] [1.10.3.—] ORF00841 1681 & 1682 cytochromed ubiquinol oxidase, subunit II (cydB) [1.10.3.—] ORF00842 1683 & 1684cyd operon protein YbgT-related protein ORF00843 1685 & 1686 cyd operonprotein YbgE ORF00844 1687 & 1688 Predicted thioesterase ORF00845 1689 &1690 TolQ protein ORF00846 1691 & 1692 TolR protein (tolR) ORF00847 1693& 1694 TolA protein ORF00848 1695 & 1696 TolA protein ORF00849 1697 &1698 tolB (tolB) ORF00850 1699 & 1700 Peptidoglycan-associatedlipoprotein precursor ORF00851 1701 & 1702 YbgF protein ORF00852 1703 &1704 quinolinate synthetase complex, A subunit (nadA) ORF00853 1705 &1706 Protein pnuC ORF00854 1707 & 1708 cation efflux family protein(efflux) ORF00855 1709 & 1710 conserved hypothetical protein ORF008561711 & 1712 phospho-2-dehydro-3-deoxyheptonate aldolase [2.5.1.54]ORF00857 1713 & 1714 hypothetical protein ORF00858 1715 & 1716Phosphoglycerate mutase 1 ORF00859 1717 & 1718 Aldose 1-epimeraseORF00860 1719 & 1720 galactokinase (galK) [2.7.1.6] ORF00861 1721 & 1722galactose-1-phosphate uridylyltransferase (galT) [2.7.7.10] ORF008621723 & 1724 UDP-glucose 4-epimerase (galE) [5.1.3.2] ORF00863 1725 &1726 ATP-binding component of molybdate transport system (atp_bind)ORF00864 1727 & 1728 hypothetical protein ORF00865 1729 & 1730Transcriptional regulator modE (modE) ORF00866 1731 & 1732 conservedhypothetical protein ORF00867 1733 & 1734 molybdate ABC transporter,periplasmic molybdate-binding protein (modA) ORF00868 1735 & 1736molybdate ABC transporter, permease protein (modB) ORF00869 1737 & 1738molybdate ABC transporter, ATP-binding protein (modC) [3.6.3.29]ORF00870 1739 & 1740 putative phosphatase ORF00871 1741 & 1742 conservedhypothetical protein ORF00872 1743 & 1744 ybhD protein ORF00873 1745 &1746 FldA protein ORF00874 1747 & 1748 2-oxoglutarate-malatetranslocator homolog yflS (SODiT1) ORF00875 1749 & 1750 aconitatehydratase, putative ORF00876 1751 & 1752 possible pectinesteraseprecursor ORF00877 1753 & 1754 conserved hypothetical protein TIGR00481ORF00878 1755 & 1756 adenosylmethionine-8-amino-7-oxononanoateaminotransferase (bioA) [2.6.1.62] ORF00879 1757 & 1758 biotin synthase(bioB) [2.8.1.6] ORF00880 1759 & 1760 8-amino-7-oxononanoate synthase(bioF) [2.3.1.47] ORF00881 1761 & 1762 biotin biosynthesis protein BioC(bioC) ORF00882 1763 & 1764 Dethiobiotin synthetase (DTBS) ORF00883 1765& 1766 Phage minor tail protein ORF00884 1767 & 1768 phage minor tailprotein L ORF00885 1769 & 1770 Putative tail fiber component K ofprophage ORF00886 1771 & 1772 Minor tail protein precursor H ORF008871773 & 1774 Phage minor tail protein ORF00888 1775 & 1776 phage minortail protein L ORF00889 1777 & 1778 Putative tail component of prophageORF00890 1779 & 1780 Phage minor tail protein L ORF00891 1781 & 1782Putative tail component of prophage ORF00892 1783 & 1784 Phage minortail protein L ORF00893 1785 & 1786 Putative tail fiber component K ofprophage ORF00894 1787 & 1788 Putative tail fiber component K ofprophage ORF00895 1789 & 1790 Bacteriophage lambda tail assembly proteinI ORF00896 1791 & 1792 host specificity protein (partial) ORF00897 1793& 1794 tail assembly protein ORF00898 1795 & 1796 Bacteriophage lambdatail assembly protein I ORF00899 1797 & 1798 host specificity protein(partial) ORF00900 1799 & 1800 Putative tail component of prophage(partial) ORF00901 1801 & 1802 hypothetical protein ORF00902 1803 & 1804conserved hypothetical protein ORF00903 1805 & 1806 conservedhypothetical protein ORF00904 1807 & 1808 conserved hypothetical proteinORF00905 1809 & 1810 SitD protein (AF128999) ORF00906 1811 & 1812 SitCprotein (AF128999) ORF00907 1813 & 1814 SitB protein (AF128999) ORF009081815 & 1816 periplasmic binding protein (chelated) ORF00909 1817 & 1818putative transposase ORF00910 1819 & 1820 conserved hypothetical proteinORF00911 1821 & 1822 isocitrate dehydrogenase [1.1.1.42] ORF00912 1823 &1824 ydfO protein ORF00913 1825 & 1826 Hypothetical transcriptionalregulator ycgE ORF00914 1827 & 1828 conserved hypothetical proteinORF00915 1829 & 1830 Sensors of blue-light using FAD family ORF009161831 & 1832 probable membrane protein b1168 (o528) ORF00917 1833 & 1834conserved hypothetical protein ORF00918 1835 & 1836 conservedhypothetical protein ORF00919 1837 & 1838 conserved hypothetical proteinORF00920 1839 & 1840 conserved hypothetical protein ORF00921 1841 & 1842putative ATP-binding component of a transport system ORF00922 1843 &1844 cell division topological specificity factor MinE (minE) ORF009231845 & 1846 cell division inhibitor MinD ORF00924 1847 & 1848 tailassembly protein ORF00925 1849 & 1850 Host specificity protein J(partial) ORF00926 1851 & 1852 host specificity protein (partial)ORF00927 1853 & 1854 excinuclease ABC, B subunit (uvrB) ORF00928 1855 &1856 Uncharacterized conserved membrane-associated protein ORF00929 1857& 1858 Molybdenum cofactor biosynthesis protein A ORF00930 1859 & 1860molybdenum cofactor biosynthesis protein B ORF00931 1861 & 1862molybdenum cofactor biosynthesis protein C (moaC) ORF00932 1863 & 1864Molybdopterin converting factor subunit 1 (MPT) ORF00933 1865 & 1866Molybdopterin converting factor subunit 2 ORF00934 1867 & 1868 conservedhypothetical protein ORF00935 1869 & 1870 membrane protein (SAD)ORF00936 1871 & 1872 hypothetical protein ORF00937 1873 & 1874Uncharacterized protein family UPF0005, putative ORF00938 1875 & 1876membrane protein, putative ORF00939 1877 & 1878 phospholipase familyprotein [2.7.8.—] ORF00940 1879 & 1880endonuclease-exonuclease-phosphatase family protein ORF00941 1881 & 1882ABC transporter, permease protein, putative ORF00942 1883 & 1884conserved hypothetical protein ORF00943 1885 & 1886 ElsD (membrane)ORF00944 1887 & 1888 ABC transporter, ATP binding-permease proteinORF00945 1889 & 1890 Hypothetical membrane protein ybhG ORF00946 1891 &1892 probable transcription regulator ybiH ORF00947 1893 & 1894Superfamily II DNA and RNA helicases ORF00948 1895 & 1896 GTPcyclohydrolase II (F160) ORF00949 1897 & 1898 probably ATP-dependenthelicase ORF00950 1899 & 1900 glycosyl transferase family protein (o320)[2.4.2.18] ORF00951 1901 & 1902 YbiC (o361) [1.1.1.—] ORF00952 1903 &1904 Protein of unknown function (DUF1471) superfamily ORF00953 1905 &1906 TraR ORF00954 1907 & 1908 iron-regulated outer membrane proteinORF00955 1909 & 1910 TonB-dependent receptor for iron transport XF0599(III) ORF00956 1911 & 1912 hypothetical protein ORF00957 1913 & 1914Protein of unknown function (DUF1471) family ORF00958 1915 & 1916Protein of unknown function (DUF890) superfamily ORF00959 1917 & 1918putative transport protein ORF00960 1919 & 1920 ATP-binding component ofglutamine high-affinity transport system (atp_bind) ORF00961 1921 & 1922amino acid ABC transporter, amino acid-binding-permease protein ORF009621923 & 1924 amino acid ABC transporter, amino acid-binding-permeaseprotein (GlnH) ORF00963 1925 & 1926 global regulator, starvationconditions ORF00964 1927 & 1928 transporter, EamA family ORF00965 1929 &1930 outer membrane protein X ORF00966 1931 & 1932 Dca ORF00967 1933 &1934 Transcriptional regulator mntR (Manganese transport regulator)ORF00968 1935 & 1936 transporter, NadC-P-Pho87 family, putative ORF009691937 & 1938 Protein ybiS precursor ORF00970 1939 & 1940 ABC transporterATP-binding protein ORF00971 1941 & 1942 hydrolase, haloaciddehalogenase-like family ORF00972 1943 & 1944 pyruvate formate-lyase[2.3.1.54] ORF00973 1945 & 1946 Iron-dependent pyruvateformate-lyase-activating enzyme (pflC) [1.97.1.4] ORF00974 1947 & 1948transaldolase, putative ORF00975 1949 & 1950 molybdopterin synthasesulfurylase MoeB (moeB) ORF00976 1951 & 1952 Molybdopterin biosynthesisprotein moeA (moeA) ORF00977 1953 & 1954 asparaginase [3.5.1.1] ORF009781955 & 1956 Hypothetical ABC transporter ATP-binding protein in bcr5′region. (D90720) ORF00979 1957 & 1958 Heme-binding protein a precursor(hemin-binding lipoprotein). ORF00980 1959 & 1960 ABC transporterpermease protein (permease) ORF00981 1961 & 1962 peptide ABCtransporter, permease protein ORF00982 1963 & 1964 EAL domain proteinORF00983 1965 & 1966 Hypothetical membrane protein yliF ORF00984 1967 &1968 MiaB-like tRNA modifying enzyme YliG, TIGR01125 ORF00985 1969 &1970 conserved hypothetical protein ORF00986 1971 & 1972 lppZ ORF009871973 & 1974 glutathione S-transferase ORF00988 1975 & 1976Penicillin-binding protein 6 precursor (PBP) [3.4.16.4] ORF00989 1977 &1978 Deoxyribose operon repressor (AE006172) ORF00990 1979 & 1980unnamed protein product ORF00991 1981 & 1982 hypothetical proteinORF00992 1983 & 1984 Multidrug translocase mdfA ORF00993 1985 & 1986conserved hypothetical protein ORF00994 1987 & 1988 hydrolase, haloaciddehalogenase-like family ORF00995 1989 & 1990 Major FacilitatorSuperfamily subfamily ORF00996 1991 & 1992 transcription regulator, TetRfamily , putative ORF00997 1993 & 1994 TrkA, Potassium channel-familyprotein ORF00998 1995 & 1996 conserved hypothetical protein ORF009991997 & 1998 Glutaredoxin, GrxA family (grxA) ORF01000 1999 & 2000Protein of unknown function (DUF1418) superfamily ORF01001 2001 & 2002Oxygen-insensitive NADPH nitroreductase [1.—.—.—] ORF01002 2003 & 2004ribosomal protein S6 modification protein ORF01003 2005 & 2006 putativesensory transduction regulator ORF01004 2007 & 2008 Putrescine-bindingperiplasmic protein precursor ORF01005 2009 & 2010 Putrescine transportATP-binding protein potG (atp_bind) ORF01006 2011 & 2012 putrescine ABCtransporter, permease protein (membrane) ORF01007 2013 & 2014 Putrescinetransport system permease protein potI ORF01008 2015 & 2016 conservedhypothetical protein ORF01009 2017 & 2018 23S rRNA(uracil-5-)-methyltransferase RumB (rumB) [2.1.1.—] ORF01010 2019 & 2020Arginine-binding periplasmic protein 2 precursor ORF01011 2021 & 2022Arginine transport system permease protein artM (membrane) ORF01012 2023& 2024 Arginine transport system permease protein artQ (artQ) ORF010132025 & 2026 Arginine-binding periplasmic protein 1 precursor ORF010142027 & 2028 Arginine transport ATP-binding protein artP ORF01015 2029 &2030 Putative lipoprotein ybjP precursor ORF01016 2031 & 2032 conservedprotein ORF01017 2033 & 2034 N-acetylmuramoyl-L-alanine amidase domainprotein [3.5.1.28] ORF01018 2035 & 2036 conserved hypothetical proteinORF01019 2037 & 2038 COG0702: Predicted nucleoside-diphosphate-sugarepimerases, putative ORF01020 2039 & 2040 Low-specificity L-threoninealdolase [4.1.2.5] ORF01021 2041 & 2042 pyruvate dehydrogenase [1.2.2.2]ORF01022 2043 & 2044 iron-sulfur cluster-binding protein ORF01023 2045 &2046 hybrid cluster protein ORF01024 2047 & 2048 putative surfaceprotein ORF01025 2049 & 2050 aquaporin Z ORF01026 2051 & 2052 homologywith RecF protein ORF01027 2053 & 2054 SomA (VirG) ORF01028 2055 & 2056Macrolide-specific efflux protein macA precursor ORF01029 2057 & 2058Macrolide-specific ABC-type efflux carrier (efflux) ORF01030 2059 & 2060Cold shock-like protein cspD ORF01031 2061 & 2062 site-specificrecombinase, phage integrase family, truncation ORF01032 2063 & 2064probable DNA binding protein -related protein ORF01033 2065 & 2066hypothetical protein ORF01034 2067 & 2068 conserved hypothetical proteinORF01035 2069 & 2070 conserved hypothetical protein ORF01036 2071 & 2072hypothetical bacteriophage protein ORF01037 2073 & 2074 Bacteriophage P4DNA primase ORF01038 2075 & 2076 Unknown protein encoded by prophageORF01039 2077 & 2078 Single-strand binding protein (SSB)(Helix-destabilizing protein) (ssb) ORF01040 2079 & 2080 hypotheticalprotein ORF01041 2081 & 2082 conserved hypothetical protein ORF010422083 & 2084 Gifsy-1 prophage protein ORF01043 2085 & 2086 Headdecoration protein (Head protein GPSHP) ORF01044 2087 & 2088hypothetical protein ORF01045 2089 & 2090 head-tail preconnector proteinGP5 ORF01046 2091 & 2092 gP1 ORF01047 2093 & 2094 Unknown proteinencoded by prophage ORF01048 2095 & 2096 Unknown protein encoded byprophage ORF01049 2097 & 2098 ATP-dependent Clp protease adaptor proteinclpS ORF01050 2099 & 2100 ATP-binding component of serine proteaseORF01051 2101 & 2102 ATP-dependent dp protease ATP-binding subunit clpAORF01052 2103 & 2104 CRISPR-associated protein Cas1 (cas1) ORF01053 2105& 2106 unnamed protein product; Similar to unknown protein ORF01054 2107& 2108 conserved hypothetical protein ORF01055 2109 & 2110 conservedhypothetical protein ORF01056 2111 & 2112 conserved hypothetical proteinORF01057 2113 & 2114 conserved hypothetical protein ORF01058 2115 & 2116conserved hypothetical protein ORF01059 2117 & 2118 translationinitiation factor IF-1 (infA) ORF01060 2119 & 2120leucyl-phenylalanyl-tRNA-protein transferase (aat) [2.3.2.—] ORF010612121 & 2122 Transport ATP-binding protein cydC ORF01062 2123 & 2124Transport ATP-binding protein cydD ORF01063 2125 & 2126 Antirestrictionprotein family ORF01064 2127 & 2128 YeeS protein (o160) ORF01065 2129 &2130 Transport ATP-binding protein cydD ORF01066 2131 & 2132thioredoxin-disulfide reductase (trxB) [1.8.1.9] ORF01067 2133 & 2134Leucine-responsive Regulatory protein ORF01068 2135 & 2136 Cell divisionprotein ftsK ORF01069 2137 & 2138 outer membrane lipoprotein carrierprotein LolA (lolA) ORF01070 2139 & 2140 unnamed protein productORF01071 2141 & 2142 seryl-tRNA synthetase (serS) [6.1.1.11] ORF010722143 & 2144 Anaerobic dimethyl sulfoxide reductase chain A precursor(dmsA) [1.8.99.—] ORF01073 2145 & 2146 anaerobic dimethylsulfoxidereductase chain B (dmsB) [1.—.—.—] ORF01074 2147 & 2148 DMSO reductaseanchor subunit (DmsC) (dmsC) [1.8.99.—] ORF01075 2149 & 2150 ProteinycaC ORF01076 2151 & 2152 Hypothetical MFS-type transporter protein ycaDORF01077 2153 & 2154 pyruvate formate lyase activating enzyme 1[1.97.1.4] ORF01078 2155 & 2156 phage integrase ORF01079 2157 & 2158phage regulatory protein ORF01080 2159 & 2160 gpB ORF01081 2161 & 2162gp80-related protein ORF01082 2163 & 2164 InsAB′ protein ORF01083 2165 &2166 InsA protein ORF01084 2167 & 2168 gp81 ORF01085 2169 & 2170 TraRprotein ORF01086 2171 & 2172 gp83 ORF01087 2173 & 2174 Replication geneA protein (GPA) ORF01088 2175 & 2176 gp91 ORF01089 2177 & 2178 conservedhypothetical protein ORF01090 2179 & 2180 hypothetical protein ORF010912181 & 2182 hypothetical protein ORF01092 2183 & 2184 phage portalprotein, PBSX family ORF01093 2185 & 2186 Putative ATPase subunit ofterminase (gpP-like) ORF01094 2187 & 2188 Phage capsid scaffoldingprotein (GPO) ORF01095 2189 & 2190 capsid scaffolding protein ORF010962191 & 2192 phage major capsid protein, P2 family ORF01097 2193 & 2194Phage small terminase subunit ORF01098 2195 & 2196 Phage head completionprotein (GPL) ORF01099 2197 & 2198 Tail protein X (GPX)-related proteinORF01100 2199 & 2200 Phage holin family 2 ORF01101 2201 & 2202 Lysozyme(Lysis protein) (Muramidase) (Endolysin)(Protein gpK) [3.2.1.17]ORF01102 2203 & 2204 hypothetical protein ORF01103 2205 & 2206 ProteinlysA ORF01104 2207 & 2208 Protein lysB (AB008550) ORF01105 2209 & 2210LysC ORF01106 2211 & 2212 Tail completion protein R (GPR) ORF01107 2213& 2214 phage virion morphogenesis protein ORF01108 2215 & 2216 gpV (gpV)ORF01109 2217 & 2218 Baseplate assembly protein W (GPW) (gpW) ORF011102219 & 2220 Baseplate J-like protein ORF01111 2221 & 2222 gpI (gpI)ORF01112 2223 & 2224 gpH ORF01113 2225 & 2226 gpG (gpG) ORF01114 2227 &2228 hypothetical protein ORF01115 2229 & 2230 Phage tail sheath proteinORF01116 2231 & 2232 phage major tail tube protein ORF01117 2233 & 2234Phage tail protein E ORF01118 2235 & 2236 phage tail tape meausureprotein, TP901 family, putative ORF01119 2237 & 2238 gpU (gpU) ORF011202239 & 2240 Gene D protein (GPD) ORF01121 2241 & 2242 Ogr ORF01122 2243& 2244 formate acetyltransferase (pflB) [2.3.1.54] ORF01123 2245 & 2246Probable formate transporter 1 ORF01124 2247 & 2248 unnamed proteinproduct ORF01125 2249 & 2250 Protein of unknown function (DUF421) familyORF01126 2251 & 2252 phosphoserine aminotransferase (serC) [2.6.1.52]ORF01127 2253 & 2254 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)[2.5.1.19] ORF01128 2255 & 2256 cmk protein precursor [3.4.24.—]ORF01129 2257 & 2258 cytidylate kinase (cmk) [2.7.4.14] ORF01130 2259 &2260 ribosomal protein S1 (rpsA) ORF01131 2261 & 2262 integration hostfactor, beta subunit (ihfB) ORF01132 2263 & 2264 DNAinternalization-related competence protein ComEC-Rec2 ORF01133 2265 &2266 lipid A export ATP-binding-permease protein MsbA (msbA) ORF011342267 & 2268 tetraacyldisaccharide 4′-kinase (lpxK) [2.7.1.130] ORF011352269 & 2270 Protein of unknown function (DUF1006) superfamily ORF011362271 & 2272 Tetraacyldisaccharide-1-P 4′-kinase ORF01137 2273 & 22743-deoxy-D-manno-octulosonate cytidylyltransferase (kdsB) [2.7.7.38]ORF01138 2275 & 2276 Uncharacterized ACR, COG1434 family ORF01139 2277 &2278 mukF protein (killing factor KicB) ORF01140 2279 & 2280 conservedhypothetical protein ORF01141 2281 & 2282 Protein smtA ORF01142 2283 &2284 Chromosome partition protein mukF (kicB) ORF01143 2285 & 2286Chromosome partition protein mukE ORF01144 2287 & 2288 cell divisionprotein mukB (mukB) ORF01145 2289 & 2290 conserved hypothetical proteinORF01146 2291 & 2292 Bacterial protein of unknown function (DUF882)superfamily ORF01147 2293 & 2294 metallo-beta-lactamase superfamilyprotein [3.—.—.—] ORF01148 2295 & 2296 Aspartate aminotransferase (aspC)[2.6.1.1] ORF01149 2297 & 2298 Outer membrane protein F precursor (GBP)ORF01150 2299 & 2300 asparaginyl-tRNA synthetase (asnS) [6.1.1.22]ORF01151 2301 & 2302 nicotinate phosphoribosyltransferase (pncB)[2.4.2.11] ORF01152 2303 & 2304 aminopeptidase N (pepN) [3.4.11.2]ORF01153 2305 & 2306 sulfonate ABC transporter, ATP-binding subunit SsuB(AF075709) ORF01154 2307 & 2308 sulfonate ABC transporter, permeaseprotein SsuC ORF01155 2309 & 2310 Alkanesulfonate monooxygenase[1.14.14.5] ORF01156 2311 & 2312 hypothetical protein ORF01157 2313 &2314 ABC transporter, aliphatic sulfonates binding protein ORF01158 2315& 2316 FMN reductase [1.5.1.29] ORF01159 2317 & 2318 dihydroorotateoxidase (pyrD) [1.3.3.1] ORF01160 2319 & 2320 Protein of unknownfunction (DUF1379) superfamily ORF01161 2321 & 2322 flavodoxin reductase(ferredoxin-NADPH reductase) family 1 ORF01162 2323 & 2324 predictedN6-adenine-specific DNA methylase ORF01163 2325 & 2326 ABC transporterATP-binding protein uup ORF01164 2327 & 2328 hypothetical proteinORF01165 2329 & 2330 Paraquat-inducible protein A ORF01166 2331 & 2332paraquat-inducible protein B ORF01167 2333 & 2334 Protein of unknownfunction (DUF330) superfamily ORF01168 2335 & 2336 Ribosome modulationfactor (Protein E)-related protein ORF01169 2337 & 2338beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabA (fabA)[4.2.1.—] ORF01170 2339 & 2340 unnamed protein product ORF01171 2341 &2342 Outer membrane protein A precursor ORF01172 2343 & 2344 Celldivision inhibitor ORF01173 2345 & 2346 hypothetical membrane protein,TIGR01666 (yccS) ORF01174 2347 & 2348 Regulator of competence-specificgenes ORF01175 2349 & 2350 Domain of unknown function ORF01176 2351 &2352 DNA helicase IV [3.6.1.—] ORF01177 2353 & 2354 methylglyoxalsynthase (mgsA) [4.2.3.3] ORF01178 2355 & 2356 Hypothetical UPF0319protein yccT precursor ORF01179 2357 & 2358 Protein yccU ORF01180 2359 &2360 hemimethylated DNA binding domain, putative ORF01181 2361 & 2362predicted SAM-dependent methyltransferase ORF01182 2363 & 2364Acylphosphatase ORF01183 2365 & 2366 probable sulfite reductase gammachain [1.8.—.—] ORF01184 2367 & 2368 Integral membrane protein ORF011852369 & 2370 Hydrogenase-1 small chain precursor ORF01186 2371 & 2372conserved hypothetical protein ORF01187 2373 & 2374 Unknown proteinencoded within prophage ORF01188 2375 & 2376 Uncharacterized BCR,COG1636 family ORF01189 2377 & 2378 putative cell killing protein ofprophage (gef) ORF01190 2379 & 2380 Protein of unknown function(DUF1277) superfamily ORF01191 2381 & 2382 endodeoxyribonuclease RUS(Holliday junction resolvase) [3.1.22.—] ORF01192 2383 & 2384Antitermination protein Q homolog of cryptic prophage ORF01193 2385 &2386 hypothetical protein ORF01194 2387 & 2388 unnamed protein product;ORF137, length ORF01195 2389 & 2390 lysis protein S.b1556 ORF01196 2391& 2392 hypothetical bacteriophage protein ORF01197 2393 & 2394 Lysozyme(Lysis protein) (Muramidase) (Endolysin) [3.2.1.17] ORF01198 2395 & 2396lipoprotein Rz1 precursor -related protein ORF01199 2397 & 2398Bacteriophage lysis protein ORF01200 2399 & 2400 Sb56 ORF01201 2401 &2402 phage terminase, small subunit, putative, P27 family, putativeORF01202 2403 & 2404 phage terminase, large subunit, putative ORF012032405 & 2406 hypothetical protein ORF01204 2407 & 2408 phage portalprotein, HK97 family ORF01205 2409 & 2410 Caudovirus prohead proteaseORF01206 2411 & 2412 phage major capsid protein, HK97 family ORF012072413 & 2414 uncharacterized phage protein (possible DNA packaging)ORF01208 2415 & 2416 phage head-tail adaptor, putative ORF01209 2417 &2418 phage protein, HK97 gp10 family ORF01210 2419 & 2420 Gp11 ORF012112421 & 2422 major tail subunit ORF01212 2423 & 2424 Phage tail assemblychaperone ORF01213 2425 & 2426 Gp14 ORF01214 2427 & 2428 tail lengthtape measure protein ORF01215 2429 & 2430 phage minor tail protein LORF01216 2431 & 2432 Putative tail fiber component K of prophageORF01217 2433 & 2434 Bacteriophage lambda tail assembly protein IORF01218 2435 & 2436 Host specificity protein J (partial) ORF01219 2437& 2438 Host specificity protein J (partial) ORF01220 2439 & 2440 hostspecificity protein (partial) ORF01221 2441 & 2442 Lom ORF01222 2443 &2444 conserved hypothetical protein ORF01223 2445 & 2446 conservedhypothetical protein ORF01224 2447 & 2448 conserved hypothetical proteinORF01225 2449 & 2450 conserved hypothetical protein ORF01226 2451 & 2452CdtA ORF01227 2453 & 2454 CdtB ORF01228 2455 & 2456 CdtC ORF01229 2457 &2458 Periplasmic solute binding protein family family ORF01230 2459 &2460 MEMBRANE PROTEIN ORF01231 2461 & 2462 Hypothetical UPF0190 proteinyedY (AE005419) ORF01232 2463 & 2464 transthyretin family proteinORF01233 2465 & 2466 Transcriptional activator protein CopR. ORF012342467 & 2468 histidine kinase [2.7.3.—] ORF01235 2469 & 2470H-NS-repressed protein, 30K (Hsp31) ORF01236 2471 & 2472 Outer membraneprotein N precursor (OmpF) ORF01237 2473 & 2474 HD domain proteinORF01238 2475 & 2476 DNA-cytosine methyltransferase (M. EcoDcm)[2.1.1.37] ORF01239 2477 & 2478 integral membrane protein ORF01240 2479& 2480 patch repair protein [3.1.—.—] ORF01241 2481 & 2482 Protein ofunknown function ORF01242 2483 & 2484 conserved hypothetical proteinORF01243 2485 & 2486 conserved hypothetical protein ORF01244 2487 & 2488diguanylate cyclase (GGDEF) domain protein ORF01245 2489 & 2490mannosyl-3-phosphoglycerate phosphatase-related protein [3.1.3.—]ORF01246 2491 & 2492 conserved hypothetical protein ORF01247 2493 & 2494conserved hypothetical protein ORF01248 2495 & 2496 Colanic acidcapsular biosynthesis activation protein A ORF01249 2497 & 2498flagellar biosynthetic protein FliR (fliR) ORF01250 2499 & 2500flagellar biosynthetic protein FliQ (fliQ) ORF01251 2501 & 2502flagellar biosynthetic protein FliP (fliP) ORF01252 2503 & 2504terminase large subunit ORF01253 2505 & 2506 phage terminase familyprotein, putative ORF01254 2507 & 2508 hypothetical protein ORF012552509 & 2510 Gp45 ORF01256 2511 & 2512 conserved hypothetical proteinORF01257 2513 & 2514 Cl protein ORF01258 2515 & 2516 transcriptionalregulator, Cro-Cl family-related protein ORF01259 2517 & 2518 repressor(cII) ORF01260 2519 & 2520 O (DNA replication;299) ORF01261 2521 & 2522P protein ORF01262 2523 & 2524 hydrogenase ORF01263 2525 & 2526Hydrogenase-1 large chain (NiFe hydrogenase) (Membrane-bound hydrogenase1 large subunit) (HYD1) (hyaB) [1.12.99.6] ORF01264 2527 & 2528Ni—Fe-hydrogenase, b-type cytochrome subunit (CybH) ORF01265 2529 & 2530Hydrogenase 1 maturation protease [3.4.24.—] ORF01266 2531 & 2532Hydrogenase-1 operon protein hyaE ORF01267 2533 & 2534 Hydrogenase-1operon protein hyaF ORF01268 2535 & 2536 cytochrome d ubiquinol oxidase,subunit I ORF01269 2537 & 2538 cytochrome d ubiquinol oxidase, subunitII (cydB) [1.10.3.—] ORF01270 2539 & 2540 conserved hypothetical proteinORF01271 2541 & 2542 cold shock-like protein ORF01272 2543 & 2544Periplasmic appA protein precursor (agp) [3.1.3.2] ORF01273 2545 & 2546hypothetical protein ORF01274 2547 & 2548 cold shock-like proteinORF01275 2549 & 2550 GnsB protein ORF01276 2551 & 2552 Putative electrontransport protein yccM ORF01277 2553 & 2554 2.7.3.— [2.7.3.—] ORF012782555 & 2556 TorCAD operon transcriptional Regulatory protein torRORF01279 2557 & 2558 Periplasmic protein torT precursor ORF01280 2559 &2560 trimethylamine-N-oxide reductase c-type cytochrome TorC (torC)ORF01281 2561 & 2562 trimethylamine-N-oxide reductase TorA (torA)[1.7.2.3] ORF01282 2563 & 2564 Chaperone protein torD ORF01283 2565 &2566 conserved hypothetical protein ORF01284 2567 & 2568 conservedhypothetical protein ORF01285 2569 & 2570 dnaJ protein ORF01286 2571 &2572 periplasmic glucose-1-phosphatase ORF01287 2573 & 2574 conservedhypothetical protein ORF01288 2575 & 2576 trp repressor-binding proteinORF01289 2577 & 2578 conserved hypothetical protein ORF01290 2579 & 2580uracil-xanthine permease ORF01291 2581 & 2582 conserved hypotheticalprotein ORF01292 2583 & 2584 uracil-xanthine permease ORF01293 2585 &2586 4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3) small chain[1.14.13.3] ORF01294 2587 & 2588 putative nitroreductase (AE005300)[1.—.—.—] ORF01295 2589 & 2590 3-oxoadipate enol-lactonehydrolase-4-carboxymuconolactone decarboxylase, putative ORF01296 2591 &2592 hypothetical protein ORF01297 2593 & 2594 endoribonuclease L-PSP,putative ORF01298 2595 & 2596 Hypothetical isochorismatase familyprotein ycdL ORF01299 2597 & 2598 bacterial luciferase family protein,putative ORF01300 2599 & 2600 transcriptional regulator, TetR family,ORF01301 2601 & 2602 prolinedehydrogenase-delta-1-pyrroline-5-carboxylate dehydrogenase ORF013022603 & 2604 conserved hypothetical protein ORF01303 2605 & 2606sodium-proline symporter (putP) ORF01304 2607 & 2608 membrane protein,putative ORF01305 2609 & 2610 putative lipoprotein ORF01306 2611 & 2612Tat-translocated enzyme ORF01307 2613 & 2614 PhoH family proteinORF01308 2615 & 2616 conserved hypothetical protein ORF01309 2617 & 2618haemin storage system, HmsR protein (IgtD) ORF01310 2619 & 2620 HmsF(HmsF) ORF01311 2621 & 2622 haemin storage system, HmsH protein ORF013122623 & 2624 GGDEF domain protein ORF01313 2625 & 2626 integrase ORF013142627 & 2628 probable membrane protein ORF01315 2629 & 2630 INSA ORF013162631 & 2632 transposase ORF01317 2633 & 2634 hypothetical proteinORF01318 2635 & 2636 Prophage CP4-57 Regulatory protein alpA ORF013192637 & 2638 conserved hypothetical protein ORF01320 2639 & 2640predicted methyltransferase ORF01321 2641 & 2642 predictedmethyltransferase ORF01322 2643 & 2644 enterotoxin ORF01323 2645 & 2646aminotransferase, class II, putative ORF01324 2647 & 2648 KbaY [4.1.2.—]ORF01325 2649 & 2650 PUTATIVE TAGATOSE 6-PHOSPHATE KINASE PROTEINORF01326 2651 & 2652 DeoR-family regulatory protein ORF01327 2653 & 2654Transcriptional regulator of sugar metabolism ORF01328 2655 & 2656D-galactarate dehydratase [4.2.1.42] ORF01329 2657 & 2658 D-GALACTARATEDEHYDRATASE [4.2.1.7] ORF01330 2659 & 2660 putative hydrolase ORF013312661 & 2662 galactarate dehydrogenase [4.2.1.42] ORF01332 2663 & 2664hypothetical protein ORF01333 2665 & 2666 conserved hypothetical proteinORF01334 2667 & 2668 Putative F1C and S fimbrial switch RegulatoryORF01335 2669 & 2670 SfaB protein ORF01336 2671 & 2672 hypotheticalprotein ORF01337 2673 & 2674 SfaD protein ORF01338 2675 & 2676 SfaEprotein ORF01339 2677 & 2678 F1C fimbrial usher ORF01340 2679 & 2680 Sfimbrial adhesin minor subunit SfaG ORF01341 2681 & 2682 S fimbrialadhesin minor subunit SfaS (pilin) ORF01342 2683 & 2684 S fimbrialadhesin minor subunit SfaH ORF01343 2685 & 2686 EAL domain, putativeORF01344 2687 & 2688 PapX protein ORF01345 2689 & 2690 hypotheticalprotein ORF01346 2691 & 2692 IroN protein ORF01347 2693 & 2694 IroEORF01348 2695 & 2696 IroD protein (Fes) ORF01349 2697 & 2698 IroC (ABC)ORF01350 2699 & 2700 Putative glucosyltransferase ORF01351 2701 & 2702conserved hypothetical protein ORF01352 2703 & 2704 conservedhypothetical protein ORF01353 2705 & 2706 unnamed protein product;ORF94, length (ISPlu9-p) ORF01354 2707 & 2708 Transposase, IS4 familyORF01355 2709 & 2710 tonB dependent outer membrane hemin receptor, hmuR(Y08983) ORF01356 2711 & 2712 conserved hypothetical protein ORF013572713 & 2714 cobalamin synthesis protein, putative ORF01358 2715 & 2716ECs1339 ORF01359 2717 & 2718 conserved hypothetical protein ORF013602719 & 2720 hypothetical protein ORF01361 2721 & 2722 replicationprotein (Rep) ORF01362 2723 & 2724 hypothetical protein ORF01363 2725 &2726 YeeP protein ORF01364 2727 & 2728 antigen43 protein orthologueORF01365 2729 & 2730 conserved hypothetical protein ORF01366 2731 & 2732Z1215 protein (o273) ORF01367 2733 & 2734 conserved hypothetical proteinORF01368 2735 & 2736 Antirestriction protein family ORF01369 2737 & 2738YeeS protein (o160) ORF01370 2739 & 2740 intergenic-regionprotein-related protein ORF01371 2741 & 2742 conserved hypotheticalprotein ORF01372 2743 & 2744 intergenic-region protein ORF01373 2745 &2746 YeeV protein ORF01374 2747 & 2748 L0008-like protein ORF01375 2749& 2750 Z1225 protein (AF071034) ORF01376 2751 & 2752 Z1226 proteinORF01377 2753 & 2754 putative dehydrogenase (AE005314) ORF01378 2755 &2756 PHP family protein ORF01379 2757 & 2758 TorD protein ORF01380 2759& 2760 Protein of unknown function (DUF1097) superfamily ORF01381 2761 &2762 Curli production assembly-transport component csgG precursorORF01382 2763 & 2764 Curli production assembly-transport component csgFprecursor ORF01383 2765 & 2766 Curli production assembly-transportcomponent csgE precursor ORF01384 2767 & 2768 transcriptional regulator,LuxR family ORF01385 2769 & 2770 curli fiber major subunit CsgA,degenerate ORF01386 2771 & 2772 curli fiber major subunit CsgA,degenerate, putative ORF01387 2773 & 2774 conserved hypothetical proteinORF01388 2775 & 2776 Appr-1-p processing enzyme family protein ORF013892777 & 2778 cardiolipin synthase ORF01390 2779 & 2780 Glucansbiosynthesis protein C [2.1.—.—] ORF01391 2781 & 2782 conservedhypothetical protein ORF01392 2783 & 2784 Periplasmic glucansbiosynthesis protein mdoG precursor ORF01393 2785 & 2786 Periplasmicglucans biosynthesis protein mdoH ORF01394 2787 & 2788 conservedhypothetical protein ORF01395 2789 & 2790 Protein of unknown function(DUF1375) superfamily ORF01396 2791 & 2792 Acidic protein msyB ORF013972793 & 2794 major facilitator family transporter ORF01398 2795 & 2796Lipid A biosynthesis lauroyl acyltransferase ORF01399 2797 & 2798rhodanese-like domain protein, putative ORF01400 2799 & 2800 Proteinycel precursor ORF01401 2801 & 2802 Cytochrome b561 homolog 2 ORF014022803 & 2804 conserved hypothetical protein ORF01403 2805 & 2806N-methyl-L-tryptophan oxidase (MTOX) [1.5.3.—] ORF01404 2807 & 2808unnamed protein product; ORF_ID: o233#8 ORF01405 2809 & 2810 unnamedprotein product; ORF_ID: o233#9 ORF01406 2811 & 2812 dihydroorotase,homodimeric type (pyrC) [3.5.2.3] ORF01407 2813 & 2814 Protein ofunknown function (DUF1439) superfamily ORF01408 2815 & 2816Glutaredoxin, GrxB family (grxB) ORF01409 2817 & 2818 Major FacilitatorSuperfamily subfamily ORF01410 2819 & 2820 Ribosomal-protein-alanineacetyltransferase [2.3.1.128] ORF01411 2821 & 2822 g20.3 ORF01412 2823 &2824 putative virulence factor ORF01413 2825 & 2826 integral membraneprotein MviN (mviN) ORF01414 2827 & 2828 Flagella synthesis protein flgNORF01415 2829 & 2830 Negative regulator of flagellin synthesis(Anti-sigma-28 factor) ORF01416 2831 & 2832 flagellar basal body P-ringprotein FlgA precursor ORF01417 2833 & 2834 flagellar basal-body rodprotein FlgB (flgB) ORF01418 2835 & 2836 flagellar basal-body rodprotein FlgC (flgC) ORF01419 2837 & 2838 flagellar biosynthesis, hookprotein ORF01420 2839 & 2840 flagellar biosynthesis, hook proteinORF01421 2841 & 2842 Flagellar basal-body rod protein flgF ORF01422 2843& 2844 Flagellar basal-body rod protein flgG (Distal rod protein)ORF01423 2845 & 2846 flagellar L-ring protein FlgH (flgH) ORF01424 2847& 2848 flagellar P-ring protein FlgI (flgI) ORF01425 2849 & 2850Peptidoglycan hydrolase flgJ [3.2.1.—] ORF01426 2851 & 2852 flagellarhook-associated protein 1 (hap1) ORF01427 2853 & 2854 Flagellarhook-associated protein 3 (hap3) ORF01428 2855 & 2856 Ribonuclease E(RNase E) (rne) [3.1.4.—] ORF01429 2857 & 2858 hypothetical proteinORF01430 2859 & 2860 ribosomal large subunit pseudouridine synthase C(orfx) [4.2.1.70] ORF01431 2861 & 2862 ribosomal large subunitpseudouridine synthase C (orfx) [4.2.1.70] ORF01432 2863 & 2864 mafprotein (maf) ORF01433 2865 & 2866 Uncharacterized ACR, COG1399 ORF014342867 & 2868 ribosomal protein L32 (rpmF) ORF01435 2869 & 28703-oxoacyl-[acyl-carrier-protein] synthase II ORF01436 2871 & 2872Aminodeoxychorismate lyase (4-amino-4-deoxychorismatelyase) (ADC lyase)(ADCL)(ADCL) [4.1.3.38] ORF01437 2873 & 2874 conserved hypotheticalprotein TIGR00247 ORF01438 2875 & 2876 thymidylate kinase (tmk)[2.7.4.9] ORF01439 2877 & 2878 DNA polymerase III, delta′ subunit[2.7.7.7] ORF01440 2879 & 2880 Putative deoxyribonuclease ycfH ORF014412881 & 2882 PTS system, glucose-specific IIBC component (ptsG)[2.7.1.69] ORF01442 2883 & 2884 FhuE receptor precursor (III) ORF014432885 & 2886 HIT-like protein ycfF (Ap4A) ORF01444 2887 & 2888 Protein ofunknown function (DUF1425) superfamily ORF01445 2889 & 2890Fibronectin-binding protein B ORF01446 2891 & 2892 unnamed proteinproduct [3.2.1.21] ORF01447 2893 & 2894 Beta-hexosaminidase ORF014482895 & 2896 ycfP protein ORF01449 2897 & 2898 NADH dehydrogenaseORF01450 2899 & 2900 conserved hypothetical protein ORF01451 2901 & 2902transcriptional regulator, TetR family ORF01452 2903 & 2904 Protein ofunknown function (DUF1471) superfamily ORF01453 2905 & 2906ErfK-YbiS-YcfS-YnhG family ORF01454 2907 & 2908 transcription-repaircoupling factor mutation frequency decline ORF01455 2909 & 2910Acyltransferase family, putative ORF01456 2911 & 2912 hypotheticalprotein ORF01457 2913 & 2914 ABC transporter, integral membrane proteinORF01458 2915 & 2916 lipoprotein releasing system, ATP-binding protein(lolD) ORF01459 2917 & 2918 lipoprotein releasing system, transmembraneprotein LolE (lolE) ORF01460 2919 & 2920 ROK family protein ORF014612921 & 2922 NAD-dependent deacetylase (Regulatory protein SIR2homolog)(DMB) [3.5.1.—] ORF01462 2923 & 2924 conserved hypothetical proteinORF01463 2925 & 2926 conserved hypothetical protein ORF01464 2927 & 2928Spermidine-putrescine-binding periplasmic protein precursor (potD)ORF01465 2929 & 2930 Spermidine-putrescine transport system permeaseprotein potC (potC) ORF01466 2931 & 2932 spermidine-putrescine transportsystem permease ORF01467 2933 & 2934 ABC transporter, polyaminetransport protein, ATP-binding protein (atp_bind) ORF01468 2935 & 2936peptidase T (pepT) [3.4.11.14] ORF01469 2937 & 2938 ycfD proteinORF01470 2939 & 2940 Sensor protein phoQ [2.7.3.—] ORF01471 2941 & 2942transcriptional regulatory protein ORF01472 2943 & 2944 adenylosuccinatelyase (purB) [4.3.2.2] ORF01473 2945 & 2946 Uncharacterized proteininvolved in purine metabolism ORF01474 2947 & 2948tRNA(5-methylaminomethyl-2-thiouridylate)-methyltransferase (trmU)[2.1.1.61] ORF01475 2949 & 2950 unnamed protein product [3.6.—.—]ORF01476 2951 & 2952 RNA pseudouridylate synthase family protein[4.2.1.70] ORF01477 2953 & 2954 conserved hypothetical protein ORF014782955 & 2956 isocitrate dehydrogenase, NADP-dependent (icd) [1.1.1.42]ORF01479 2957 & 2958 Prophage lambda integrase ORF01480 2959 & 2960conserved hypothetical protein ORF01481 2961 & 2962 unnamed proteinproduct; Some similarities with bacteriophage protein ORF01482 2963 &2964 exonuclease Z0951 [similarity] [3.1.11.3] ORF01483 2965 & 2966recombination protein Bet ORF01484 2967 & 2968 Host-nuclease inhibitorprotein Gam ORF01485 2969 & 2970 Kil protein ORF01486 2971 & 2972 LambdaRegulatory protein CIII-related protein ORF01487 2973 & 2974 Ea10 geneprotein ORF01488 2975 & 2976 conserved hypothetical protein ORF014892977 & 2978 antitermination protein Q ORF01490 2979 & 2980 lysozyme[3.2.1.17] ORF01491 2981 & 2982 Bacteriophage lysis protein ORF014922983 & 2984 hypothetical protein ORF01493 2985 & 2986 prophage L54a, HNHendonuclease family protein ORF01494 2987 & 2988 small terminase subunitORF01495 2989 & 2990 phage terminase, large subunit, putative ORF014962991 & 2992 conserved hypothetical protein ORF01497 2993 & 2994 phageportal protein, HK97 family ORF01498 2995 & 2996 Sb5 (AF181080) ORF014992997 & 2998 phage major capsid protein, HK97 family ORF01500 2999 & 3000Sb7-related protein ORF01501 3001 & 3002 Sb8 ORF01502 3003 & 3004 Sb9ORF01503 3005 & 3006 hypothetical protein ORF01504 3007 & 3008 Sb11ORF01505 3009 & 3010 conserved hypothetical protein ORF01506 3011 & 3012Bacteriophage Mu tail sheath protein (GpL) ORF01507 3013 & 3014 Sb14ORF01508 3015 & 3016 hypothetical protein ORF01509 3017 & 3018 Sb15ORF01510 3019 & 3020 tail protein ORF01511 3021 & 3022 tail-DNAcirculation protein (muN) ORF01512 3023 & 3024 Bacteriophage Mu Pprotein ORF01513 3025 & 3026 Bacteriophage Mu Gp45 protein ORF01514 3027& 3028 Phage protein GP46 ORF01515 3029 & 3030 tail protein ORF015163031 & 3032 tail protein ORF01517 3033 & 3034 ycfE protein (fragment)ORF01518 3035 & 3036 Domain of unknown function DUF144 superfamilyORF01519 3037 & 3038 unnamed protein product; unidentified reading frameP-293 ORF01520 3039 & 3040 lipoprotein Rz1 precursor -related proteinORF01521 3041 & 3042 Bacteriophage lysis protein ORF01522 3043 & 3044Bor protein homolog from lambdoid prophage DLP12 ORF01523 3045 & 3046hypothetical protein ORF01524 3047 & 3048 conserved hypothetical proteinORF01525 3049 & 3050 Bacteriophage lambda head decoration protein DORF01526 3051 & 3052 Phage major capsid protein E ORF01527 3053 & 3054conserved hypothetical protein ORF01528 3055 & 3056 Phage Head-TailAttachment ORF01529 3057 & 3058 Prophage minor tail protein Z (GPZ)ORF01530 3059 & 3060 Minor tail protein U ORF01531 3061 & 3062hypothetical protein ORF01532 3063 & 3064 Minor tail protein Z (GPZ)ORF01533 3065 & 3066 Phage minor tail protein U ORF01534 3067 & 3068minor tail protein ORF01535 3069 & 3070 Phage minor tail protein UORF01536 3071 & 3072 major tail protein ORF01537 3073 & 3074 phage minortail protein G ORF01538 3075 & 3076 minor tail protein ORF01539 3077 &3078 minor tail protein ORF01540 3079 & 3080 tail length tape measureprotein precursor ORF01541 3081 & 3082 minor tail protein ORF01542 3083& 3084 Minor tail protein precursor H ORF01543 3085 & 3086 Minor tailprotein precursor H ORF01544 3087 & 3088 hypothetical bacteriophageprotein ORF01545 3089 & 3090 unnamed protein product; Similar to tailfiber assembly protein ORF01546 3091 & 3092 Protease VII precursor(Omptin) (Outer membrane protein3B) (Protease A) (SopA) [3.4.21.87]ORF01547 3093 & 3094 Protease VII precursor (Omptin) (Outer membraneprotein3B) (Protease A) (SopA) [3.4.21.87] ORF01548 3095 & 3096 ProteaseVII precursor (Omptin) (Outer membrane protein3B) (Protease A) (SopA)[3.4.21.87] ORF01549 3097 & 3098 ydfE protein (35kD) ORF01550 3099 &3100 Phage terminase large subunit (GpA) ORF01551 3101 & 3102 Phageterminase large subunit (GpA) ORF01552 3103 & 3104 Phage terminase largesubunit (GpA) ORF01553 3105 & 3106 Portal protein (Head protein GP4)ORF01554 3107 & 3108 head-tail preconnector protein GP5 ORF01555 3109 &3110 Head-tail preconnector protein GP5 [Contains: Scaffold proteinGP6(Head protein GP6)] ORF01556 3111 & 3112 Bacteriophage lambda headdecoration protein D ORF01557 3113 & 3114 Phage major capsid protein EORF01558 3115 & 3116 conserved hypothetical protein ORF01559 3117 & 3118Phage Head-Tail Attachment ORF01560 3119 & 3120 Prophage minor tailprotein Z (GPZ) ORF01561 3121 & 3122 Phage minor tail protein U ORF015623123 & 3124 major tail protein ORF01563 3125 & 3126 phage minor tailprotein G ORF01564 3127 & 3128 minor tail protein ORF01565 3129 & 3130Portal protein (Head protein GP4) ORF01566 3131 & 3132 head-tailpreconnector protein GP5 ORF01567 3133 & 3134 Head-tail preconnectorprotein GP5 [Contains: Scaffold protein GP6(Head protein GP6)] ORF015683135 & 3136 Head-tail preconnector protein GP5 [Contains: Scaffoldprotein GP6(Head protein GP6)] ORF01569 3137 & 3138 Bacteriophage lambdahead decoration protein D ORF01570 3139 & 3140 phage tail tape measureprotein, lambda family ORF01571 3141 & 3142 Phage minor tail proteinORF01572 3143 & 3144 minor tail protein ORF01573 3145 & 3146 Putativetail component of prophage ORF01574 3147 & 3148 Phage minor tail proteinORF01575 3149 & 3150 Phage minor tail protein L ORF01576 3151 & 3152Putative tail component of prophage ORF01577 3153 & 3154 Phage minortail protein ORF01578 3155 & 3156 Phage minor tail protein L ORF015793157 & 3158 transcriptional regulator, GntR family ORF01580 3159 & 3160Probable oxidoreductase ydfG ORF01581 3161 & 3162 peptidyl-dipeptidaseDcp, putative ORF01582 3163 & 3164 peptidyl-dipeptidase Dcp ORF015833165 & 3166 hypothetical protein ORF01584 3167 & 3168 GGDEF domainprotein ORF01585 3169 & 3170 hypothetical protein ORF01586 3171 & 3172Major Facilitator Superfamily subfamily ORF01587 3173 & 3174 YdeDORF01588 3175 & 3176 Putative conserved protein ORF01589 3177 & 3178 PTSsystem, cellobiose-specific IIB component (PTS) [2.7.1.69] ORF01590 3179& 3180 Putative conserved protein ORF01591 3181 & 3182 PTS system,cellobiose-specific IIA component ORF01592 3183 & 3184 f538 ORF015933185 & 3186 6-phospho-beta-glucosidase ORF01594 3187 & 3188 Multipleantibiotic resistance protein marB-related protein ORF01595 3189 & 3190multiple antibiotic resistance protein MarA ORF01596 3191 & 3192Multiple antibiotic resistance protein marR (AL359989) ORF01597 3193 &3194 conserved hypothetical protein TIGR00427 ORF01598 3195 & 3196 Sugarefflux transporter ORF01599 3197 & 3198 transcriptional regulator, LysRfamily ORF01600 3199 & 3200 Aldehyde-dehydrogenase like protein ynel(SSDH) [1.2.1.—] ORF01601 3201 & 3202 glutaminase family protein,interruption-N ORF01602 3203 & 3204 conserved hypothetical proteinORF01603 3205 & 3206 GGDEF domain protein ORF01604 3207 & 3208 altronateoxidoreductase ORF01605 3209 & 3210 Uncharacterised protein family(UPF0187) superfamily ORF01606 3211 & 3212 trans-aconitatemethyltransferase [2.1.1.144] ORF01607 3213 & 3214 Protein hipB ORF016083215 & 3216 Protein hipA ORF01609 3217 & 3218 conserved hypotheticalprotein ORF01610 3219 & 3220 short chain dehydrogenase ORF01611 3221 &3222 Type-1 fimbrial protein, A chain precursor ORF01612 3223 & 3224Chaperone protein fimC precursor ORF01613 3225 & 3226 Outer membraneusher protein fimD precursor ORF01614 3227 & 3228 fimbrial morphologyORF01615 3229 & 3230 Hypothetical fimbrial-like protein ydeQ precursorORF01616 3231 & 3232 oxidoreductase alpha (molybdopterin) subunitORF01617 3233 & 3234 HTH-type transcriptional regulator ydeO ORF016183235 & 3236 arylsulfatase B (GALNS) ORF01619 3237 & 3238 radical SAMdomain protein protein ORF01620 3239 & 3240 ABC efflux transporter,permease-ATP-binding protein, putative ORF01621 3241 & 3242 cds103ORF01622 3243 & 3244 cds103 ORF01623 3245 & 3246 peptidase, M16 family,putative [3.4.99.—] ORF01624 3247 & 3248 glutamate decarboxylase[4.1.1.15] ORF01625 3249 & 3250 Amino acid antiporter ORF01626 3251 &3252 yngK protein ORF01627 3253 & 3254 Putative conservedprotein-related protein ORF01628 3255 & 3256 Sensor protein FixL (EC2.7.3.—). (nifL) [2.7.3.—] ORF01629 3257 & 3258 protein C, osmoticallyinducible ORF01630 3259 & 3260 hypothetical protein ORF01631 3261 & 3262conserved hypothetical protein ORF01632 3263 & 3264 conservedhypothetical protein ORF01633 3265 & 3266 30S ribosomal protein S22(Stationary-phase-induced ribosome-associated protein) (SRA) (ProteinD)-related protein ORF01634 3267 & 3268 hypothetical protein ORF016353269 & 3270 NAD-linked malate dehydrogenase (malic enzyme) ORF01636 3271& 3272 alcohol dehydrogenase, propanol-preferring (adhA) [1.—.—.—]ORF01637 3273 & 3274 proteic killer suppression protein hig A ORF016383275 & 3276 conserved hypothetical protein ORF01639 3277 & 3278 proteickiller active protein hig B ORF01640 3279 & 3280 formate dehydrogenase,gamma subunit [1.2.1.2] ORF01641 3281 & 3282 formate dehydrogenase, betasubunit (FdxH) [1.2.1.2] ORF01642 3283 & 3284 formate dehydrogenase,alpha subunit, selenocysteine-containing [1.2.1.2] ORF01643 3285 & 3286formate dehydrogenase, alpha subunit, selenocysteine-containing[1.2.1.2] ORF01644 3287 & 3288 formate dehydrogenase, alpha subunit,selenocysteine-containing [1.2.1.2] ORF01645 3289 & 3290 hypotheticalprotein ORF01646 3291 & 3292 membrane protein yddG ORF01647 3293 & 3294nitrite extrusion protein 2 ORF01648 3295 & 3296 nitrate reductase,alpha subunit [1.7.99.4] ORF01649 3297 & 3298 nitrate reductase, betasubunit (narH) [1.7.99.4] ORF01650 3299 & 3300 Respiratory nitratereductase 2 delta chain [1.7.99.4] ORF01651 3301 & 3302 respiratorynitrate reductase, gamma subunit (narI) [1.7.99.4] ORF01652 3303 & 3304phenazine biosynthesis protein PhzF family subfamily ORF01653 3305 &3306 Chain D, M. Smegmatis Arylamine N-Acetyl Transferase [2.3.1.118]ORF01654 3307 & 3308 Flavin reductase like domain protein ORF01655 3309& 3310 H repeat-associated protein (ORF-H) ORF01656 3311 & 3312conserved hypothetical protein ORF01657 3313 & 3314 conservedhypothetical protein ORF01658 3315 & 3316 Aec15 (AF044503) ORF01659 3317& 3318 Uncharacterized conserved protein ORF01660 3319 & 3320 probabletransferase (U59485) ORF01661 3321 & 3322 glutathione S-transferase gstORF01662 3323 & 3324 L-asparagine permease (516aa) ORF01663 3325 & 3326conserved hypothetical protein ORF01664 3327 & 3328 TonB-dependentreceptor protein ORF01665 3329 & 3330 Putative conserved protein-relatedprotein ORF01666 3331 & 3332 acetyltransferase, GNAT family [2.3.1.—]ORF01667 3333 & 3334 Protein of unknown function, DUF606 superfamilyORF01668 3335 & 3336 conserved hypothetical protein ORF01669 3337 & 3338conserved hypothetical protein ORF01670 3339 & 3340 hypothetical proteinORF01671 3341 & 3342 aldehyde dehydrogenase family protein (BADH)[1.2.1.8] ORF01672 3343 & 3344 polyamine ABC transporter, permeaseprotein ORF01673 3345 & 3346 polyamine ABC transporter, permease proteinORF01674 3347 & 3348 polyamine ABC transporter, ATP-binding proteinORF01675 3349 & 3350 polyamine ABC transporter, periplasmicpolyamine-binding protein ORF01676 3351 & 3352 unnamed protein product(TAT) [2.6.1.5] ORF01677 3353 & 3354 unnamed protein product ORF016783355 & 3356 conserved hypothetical protein ORF01679 3357 & 3358peptidase, U32 family ORF01680 3359 & 3360 transcriptional regulator,Cro-Cl family, putative ORF01681 3361 & 3362 similar to benzoatetransport protein ORF01682 3363 & 3364 Hypothetical lipoprotein ydcLprecursor ORF01683 3365 & 3366 tellurite resistance protein TehB (tehB)ORF01684 3367 & 3368 Tellurite resistance protein tehA ORF01685 3369 &3370 ribosomal-protein-serine acetyltransferase, putative ORF01686 3371& 3372 conserved hypothetical protein ORF01687 3373 & 3374 Protein ofunknown function (DUF465) family ORF01688 3375 & 3376 Glucansbiosynthesis protein D precursor ORF01689 3377 & 3378 Protein of unknownfunction (DUF1338) family ORF01690 3379 & 3380 transcriptional regulatorPcaQ ORF01691 3381 & 3382 methyl-accepting chemotaxis protein II,aspartate sensor receptor ORF01692 3383 & 3384 Hok protein.-relatedprotein ORF01693 3385 & 3386 conserved hypothetical protein ORF016943387 & 3388 Cytochrome b561 (561) ORF01695 3389 & 3390glyceraldehyde-3-phosphate dehydrogenase, type I (gap) [1.2.1.—]ORF01696 3391 & 3392 Aldehyde dehydrogenase A (NAD) [1.2.1.22] ORF016973393 & 3394 Protein ydcF ORF01698 3395 & 3396 ATP-dependent helicaseHrpA (hrpA) ORF01699 3397 & 3398 [acyl-carrier-protein]phosphodiesterase acpD (fragment) [3.1.4.14] ORF01700 3399 & 3400 Dualspecificity phosphatase, catalytic domain protein ORF01701 3401 & 3402Lysophospholipase ORF01702 3403 & 3404 phosphatidatecytidylyltransferase (cdsA) [2.7.7.41] ORF01703 3405 & 3406 CDP-alcoholphosphatidyltransferase family ORF01704 3407 & 3408 oxidoreductase,aldo-keto reductase family [1.—.—.—] ORF01705 3409 & 3410 split orfORF01706 3411 & 3412 Protein of unknown function (DUF1318) familyORF01707 3413 & 3414 lipoprotein, putative ORF01708 3415 & 3416 unnamedprotein product; Similar to membrane protein YdbH of Escherichia coliORF01709 3417 & 3418 fermentative D-lactate dehydrogenase (D-LDH)[1.1.1.28] ORF01710 3419 & 3420 Heat shock protein hslJ ORF01711 3421 &3422 lipoprotein, putative ORF01712 3423 & 3424 pyruvate: ferredoxin(flavodoxin) oxidoreductase (nifJ) [1.2.7.1] ORF01713 3425 & 3426 Outermembrane protein N precursor (lbc) ORF01714 3427 & 3428 putativefilament protein ORF01715 3429 & 3430 integrase for bacteriophageBP-933W ORF01716 3431 & 3432 YdaO protein ORF01717 3433 & 3434ATP-independent RNA helicase dbpA ORF01718 3435 & 3436 hypotheticalprotein ORF01719 3437 & 3438 CorA-like Mg2+ transporter protein ORF017203439 & 3440 Diguanylate cyclase-phosphodiesterase domain 1 (GGDEF)ORF01721 3441 & 3442 Smr domain protein ORF01722 3443 & 3444 drugresistance transporter, Bcr-CflA family (emrB) ORF01723 3445 & 3446 HlyDfamily secretion protein ORF01724 3447 & 3448 transcriptional regulator,AraC family ORF01725 3449 & 3450 conserved hypothetical protein ORF017263451 & 3452 Methylated-DNA--protein-cysteine methyltransferase(6-O-methylguanine-DNA methyltransferase) (MGMT)(O-6-methylguanine-DNA-alkyltransferase) [2.1.1.63] ORF01727 3453 & 3454Fumarate and nitrate reduction Regulatory protein ORF01728 3455 & 3456universal stress protein family domain protein ORF01729 3457 & 3458conserved hypothetical protein ORF01730 3459 & 3460 small-conductancemechanosensitive channel ORF01731 3461 & 3462 Periplasmic mureinpeptide-binding protein precursor ORF01732 3463 & 3464 Xanthosine operonregulatory protein. ORF01733 3465 & 3466 dienelactone hydrolase domainprotein ORF01734 3467 & 3468 conserved hypothetical protein ORF017353469 & 3470 Mandelate racemase - muconate lactonizing enzyme, C-terminaldomain protein ORF01736 3471 & 3472 YcjI ORF01737 3473 & 3474 thiolperoxidase (P20) [1.11.1.—] ORF01738 3475 & 3476 TranscriptionalRegulatory protein tyrR ORF01739 3477 & 3478 conserved hypotheticalprotein TIGR01620 ORF01740 3479 & 3480 YcjX (AY008264) ORF01741 3481 &3482 transcription regulator homolog lin2974 ORF01742 3483 & 3484 Outermembrane protein G precursor ORF01743 3485 & 3486 ABC transporter domainprotein ORF01744 3487 & 3488 beta-phosphoglucomutase (pgmB) [5.4.2.6]ORF01745 3489 & 3490 maltose phosphorylase homolog lin2966(phosphorylase) [3.2.1.—] ORF01746 3491 & 3492 oxidoreductase,Gfo-Idh-MocA family ORF01747 3493 & 3494 an E. coli protein homologlin2968 [5.3.1.—] ORF01748 3495 & 3496 Alcohol dehydrogenase (EC1.1.1.1). [1.1.1.—] ORF01749 3497 & 3498 ABC transporter, permeaseprotein (AF175299) ORF01750 3499 & 3500 Sn-glycerol-3-phosphatetransport system permease protein UgpA. (AF307052) ORF01751 3501 & 3502ABC transporter, substrate-binding protein ORF01752 3503 & 3504dextransucrase (EC 2.4.1.5) , putative [2.4.1.5] ORF01753 3505 & 3506rhodanese-like domain protein ORF01754 3507 & 3508 phage shockprotein-related protein ORF01755 3509 & 3510 Phage shock protein CORF01756 3511 & 3512 phage shock protein B ORF01757 3513 & 3514 Phageshock protein A ORF01758 3515 & 3516 Psp operon transcriptionalactivator ORF01759 3517 & 3518 probable amino acid permease ycjJORF01760 3519 & 3520 conserved hypothetical protein ORF01761 3521 & 3522Peptide transport periplasmic protein sapA precursor ORF01762 3523 &3524 Peptide transport system permease protein sapB ORF01763 3525 & 3526Peptide transport system permease protein sapC (sapC) ORF01764 3527 &3528 Peptide transport system ATP-binding protein sapD ORF01765 3529 &3530 Peptide transport system ATP-binding protein sapF ORF01766 3531 &3532 drug resistance transporter, Bcr-CflA family protein ORF01767 3533& 3534 EefC outer membrane protein ORF01768 3535 & 3536 Acriflavineresistance protein B ORF01769 3537 & 3538 Acriflavine resistance proteinA precursor (MFP) ORF01770 3539 & 3540 transcriptional regulator, TetRfamily ORF01771 3541 & 3542 enoyl-[acyl-carrier-protein] reductaseECs1861 (NADH) [1.3.1.9] ORF01772 3543 & 3544 conserved hypotheticalprotein ORF01773 3545 & 3546 exoribonuclease II (rnb) [3.1.13.1]ORF01774 3547 & 3548 sensory box protein ORF01775 3549 & 3550 conservedhypothetical protein ORF01776 3551 & 3552 glycerol-3-phosphate regulonrepressor, putative ORF01777 3553 & 3554 Osmotically induciblelipoprotein B precursor-related protein ORF01778 3555 & 3556 translationinitation factor SUI1, putative ORF01779 3557 & 3558 orotidine5′-phosphate decarboxylase (pyrF) [4.1.1.23] ORF01780 3559 & 3560hypothetical protein ORF01781 3561 & 3562 predicted N-acetylglucosaminyltransferase ORF01782 3563 & 3564 unnamed protein product ORF01783 3565 &3566 Phosphatidylglycerophosphatase B (pgpB) [3.1.3.27] ORF01784 3567 &3568 GTP cyclohydrolase II (ribA) [3.5.4.25] ORF01785 3569 & 3570aconitate hydratase 1 (acnA) [4.2.1.3] ORF01786 3571 & 3572 conservedhypothetical protein ORF01787 3573 & 3574 conserved hypothetical proteinORF01788 3575 & 3576 HTH-type transcriptional regulator cysB (Cysregulon transcriptionalactivator) ORF01789 3577 & 3578 DNA topoisomeraseI ORF01790 3579 & 3580 hypothetical protein ORF01791 3581 & 3582 ProteinyciN ORF01792 3583 & 3584 Possible protease sohB ORF01793 3585 & 3586Oxidoreductase [1.—.—.—] ORF01794 3587 & 3588 cob(I)alaminadenosyltransferase (cobO) [2.5.1.17] ORF01795 3589 & 3590 RNApseudouridylate synthase family protein [4.2.1.70] ORF01796 3591 & 3592conserved hypothetical protein ORF01797 3593 & 3594 Sua5-YciO-YrdC-YwlCfamily protein ORF01798 3595 & 3596 Protein trpH ORF01799 3597 & 3598anthranilate synthase component I (trpE) [4.1.3.27] ORF01800 3599 & 3600Anthranilate synthase component II [4.1.3.27] ORF01801 3601 & 3602anthranilate isomerase (PRAI) [4.1.1.48] ORF01802 3603 & 3604 tryptophansynthase, beta subunit (trpB) [4.2.1.20] ORF01803 3605 & 3606 tryptophansynthase, alpha subunit (trpA) [4.2.1.20] ORF01804 3607 & 3608 conservedhypothetical protein ORF01805 3609 & 3610 Protein yciF ORF01806 3611 &3612 yciE ORF01807 3613 & 3614 Outer membrane protein W precursorORF01808 3615 & 3616 Tail fiber assembly protein homolog from lambdoidprophage ORF01809 3617 & 3618 Tail fiber assembly protein homolog fromlambdoid prophage Qin ORF01810 3619 & 3620 probable membrane protein ofprophage CP-933X Z1918 ORF01811 3621 & 3622 probable membrane protein ofprophage CP-933X Z1918 ORF01812 3623 & 3624 unknown protein encoded byprophage CP-933X ORF01813 3625 & 3626 hypothetical protein ORF01814 3627& 3628 Lom ORF01815 3629 & 3630 host specificity protein (partial)ORF01816 3631 & 3632 Host specificity protein J (partial) ORF01817 3633& 3634 Bacteriophage lambda tail assembly protein I ORF01818 3635 & 3636Putative tail fiber component K of prophage ORF01819 3637 & 3638 minortail protein ORF01820 3639 & 3640 minor tail protein ORF01821 3641 &3642 Phage minor tail protein ORF01822 3643 & 3644 Minor tail proteinprecursor H ORF01823 3645 & 3646 minor tail protein ORF01824 3647 & 3648phage minor tail protein G ORF01825 3649 & 3650 Bacterial Ig-like domain(group 2) family ORF01826 3651 & 3652 Phage minor tail protein UORF01827 3653 & 3654 Prophage minor tail protein Z (GPZ) ORF01828 3655 &3656 Phage Head-Tail Attachment ORF01829 3657 & 3658 conservedhypothetical protein ORF01830 3659 & 3660 Phage major capsid protein EORF01831 3661 & 3662 Bacteriophage lambda head decoration protein DORF01832 3663 & 3664 head-tail preconnector protein GP5 ORF01833 3665 &3666 phage portal protein, lambda family ORF01834 3667 & 3668 gpWORF01835 3669 & 3670 Phage terminase large subunit (GpA) ORF01836 3671 &3672 Terminase small subunit (GP1) (fragment) ORF01837 3673 & 3674hypothetical protein ORF01838 3675 & 3676 Bacteriophage lysis proteinORF01839 3677 & 3678 lipoprotein Rz1 precursor -related protein ORF018403679 & 3680 putative bacteriophage protein ORF01841 3681 & 3682 Lysozyme(Lysis protein) (Muramidase) (Endolysin) [3.2.1.17] ORF01842 3683 & 3684Protein of unknown function (DUF1327) superfamily ORF01843 3685 & 3686Lysis protein S homolog from lambdoid prophage ORF01844 3687 & 3688unnamed protein product; ORF616 ORF01845 3689 & 3690 unnamed proteinproduct; ORF616 ORF01846 3691 & 3692 DNA adenine-methylase ORF01847 3693& 3694 unknown protein encoded by prophage CP-933O ORF01848 3695 & 3696Antitermination protein Q homolog of cryptic prophage ORF01849 3697 &3698 endodeoxyribonuclease RUS (Holliday junction resolvase) [3.1.22.—]ORF01850 3699 & 3700 Protein of unknown function (DUF1277) superfamilyORF01851 3701 & 3702 prophage maintenance protein [similarity]-relatedprotein ORF01852 3703 & 3704 hypothetical protein ORF01853 3705 & 3706conserved hypothetical protein ORF01854 3707 & 3708 Unknown proteinencoded within prophage ORF01855 3709 & 3710 conserved hypotheticalprotein ORF01856 3711 & 3712 Unknown protein encoded by cryptic prophageORF01857 3713 & 3714 Peptidase S24-like domain, putative ORF01858 3715 &3716 Protein of unknown function (DUF1391) family ORF01859 3717 & 3718ydfB protein-related protein ORF01860 3719 & 3720 conserved hypotheticalprotein ORF01861 3721 & 3722 hypothetical protein ORF01862 3723 & 3724Division inhibition protein dicB ORF01863 3725 & 3726 unknown proteinencoded by prophage CP-933O -related protein ORF01864 3727 & 3728Exodeoxyribonuclease VIII (35kD) [3.1.11.—] ORF01865 3729 & 3730integrase ORF01866 3731 & 3732 Outer membrane protein W precursorORF01867 3733 & 3734 yciC ORF01868 3735 & 3736 intracellular septationprotein A (ispZ) ORF01869 3737 & 3738 acyl-CoA hydrolase [3.1.2.—]ORF01870 3739 & 3740 TonB protein ORF01871 3741 & 3742 yciI proteinORF01872 3743 & 3744 Putative potassium channel protein ORF01873 3745 &3746 Cardiolipin synthetase (Cardiolipin synthase) (CLsynthase)[2.7.8.—] ORF01874 3747 & 3748 Protein of unknown function, DUF440superfamily ORF01875 3749 & 3750 peptide ABC transporter, ATP-bindingprotein (AA1) ORF01876 3751 & 3752 oligopeptide ABC transporter,ATP-binding protein (AA1) ORF01877 3753 & 3754 oligopeptide ABCtransporter, permease protein (AA1) ORF01878 3755 & 3756 Oligopeptidetransport system permease protein oppB (AA1) ORF01879 3757 & 3758Periplasmic oligopeptide-binding protein precursor ORF01880 3759 & 3760membrane protein, putative ORF01881 3761 & 3762 acetaldehydedehydrogenase (acetylating) - alcohol dehydrogenase (EC 1.1.1.1) (ACDH)[1.2.1.10] ORF01882 3763 & 3764 Transposase insG for insertion sequenceelement IS4 ORF01883 3765 & 3766 thymidine kinase [2.7.1.21] ORF018843767 & 3768 conserved hypothetical protein ORF01885 3769 & 3770DNA-binding protein H—NS homolog virR ORF01886 3771 & 3772UTP-glucose-1-phosphate uridylyltransferase (galU) [2.7.7.9] ORF018873773 & 3774 Hnr protein ORF01888 3775 & 3776 drug transporter ORF018893777 & 3778 unnamed protein product ORF01890 3779 & 3780formyltetrahydrofolate deformylase (purU) [3.5.1.10] ORF01891 3781 &3782 Tpn1330 protein ORF01892 3783 & 3784 respiratory nitrate reductase,gamma subunit (narI) [1.7.99.4] ORF01893 3785 & 3786 Respiratory nitratereductase 1 delta chain [1.7.99.4] ORF01894 3787 & 3788 nitratereductase, beta subunit (narH) [1.7.99.4] ORF01895 3789 & 3790 nitratereductase, alpha subunit [1.7.99.4] ORF01896 3791 & 3792 Nitriteextrusion protein 1 ORF01897 3793 & 3794 Nitrate-nitrite sensor proteinnarX [2.7.3.—] ORF01898 3795 & 3796 narL ORF01899 3797 & 3798 conservedhypothetical protein ORF01900 3799 & 3800 YchN protein ORF01901 3801 &3802 Cation transport protein chaC ORF01902 3803 & 3804 Cation transportregulator chaB-related protein ORF01903 3805 & 3806 Calcium-protonantiporter ORF01904 3807 & 3808 conserved hypothetical protein ORF019053809 & 3810 3-deoxy-8-phosphooctulonate synthase (kdsA) [2.5.1.55]ORF01906 3811 & 3812 Protein sirB1 ORF01907 3813 & 3814 Protein sirB1ORF01908 3815 & 3816 Protein sirB2 ORF01909 3817 & 3818 Proteinmethyltransferase hemK (hemK) [2.1.1.—] ORF01910 3819 & 3820 peptidechain release factor 1 (prfA) ORF01911 3821 & 3822 glutamyl-tRNAreductase (hemA) [1.2.1.—] ORF01912 3823 & 3824 outer membranelipoprotein LolB (lolB) ORF01913 3825 & 38264-diphosphocytidyl-2C-methyl-D-erythritol kinase (ispE) [2.7.1.148]ORF01914 3827 & 3828 Ribose-phosphate pyrophosphokinase ORF01915 3829 &3830 sulfate permease family protein ORF01916 3831 & 3832 conservedhypothetical protein ORF01917 3833 & 3834 peptidyl-tRNA hydrolase (pth)[3.1.1.29] ORF01918 3835 & 3836 GTP-binding protein YchF (ychF) ORF019193837 & 3838 Glycerol metabolism operon Regulatory protein ORF01920 3839& 3840 dihydroxyacetone kinase, DhaK subunit (dhaK) [2.7.1.—] ORF019213841 & 3842 dihydroxyacetone kinase, L subunit [2.7.1.—] ORF01922 3843 &3844 phosphoenolpyruvate-protein phosphotransferase, El-HPr-EllAcomponents ORF01923 3845 & 3846 hypothetical protein ORF01924 3847 &3848 conserved hypothetical protein ORF01925 3849 & 3850 Trehalase[3.2.1.28] ORF01926 3851 & 3852 Periplasmic binding protein familyORF01927 3853 & 3854 Iron (III) ORF01928 3855 & 3856 conservedhypothetical protein ORF01929 3857 & 3858 possible ABC-typeiron-siderophore transport system ATP-binding protein (III) ORF019303859 & 3860 conserved hypothetical protein ORF01931 3861 & 3862 modDprotein (modD) ORF01932 3863 & 3864 Putative outer membrane receptor,probably tonB dependent ORF01933 3865 & 3866 Transglycosylase associatedprotein ORF01934 3867 & 3868 YcgR protein superfamily ORF01935 3869 &3870 murein transglycosylase E ORF01936 3871 & 3872 Muramoyltetrapeptidecarboxypeptidase (LD-carboxypeptidase A) [3.4.17.13] ORF01937 3873 &3874 Na+—H+ antiporter ORF01938 3875 & 3876 alanine racemase (alr)[5.1.1.1] ORF01939 3877 & 3878 D-amino acid dehydrogenase small subunitORF01940 3879 & 3880 unidentified protein ORF01941 3881 & 3882 negativeregulator for fad regulon, and positive ORF01942 3883 & 3884 Na+—H+antiporter NhaB (nhaB) ORF01943 3885 & 3886 Disulfide bond formationprotein B [1.8.4.—] ORF01944 3887 & 3888 UmuC protein ORF01945 3889 &3890 UmuD protein (R391) [3.4.21.—] ORF01946 3891 & 3892 cytolysin AORF01947 3893 & 3894 Bacterial protein of unknown function (DUF838)superfamily ORF01948 3895 & 3896 Protein ycgM (FAA) ORF01949 3897 & 3898Protein ycgL ORF01950 3899 & 3900 Fels-1 Prophage Protein-like familyORF01951 3901 & 3902 septum site-determining protein MinC (minC)ORF01952 3903 & 3904 Lom ORF01953 3905 & 3906 Putative tail component ofprophage (partial) ORF01954 3907 & 3908 Lom ORF01955 3909 & 3910Putative tail component of prophage (partial) ORF01956 3911 & 3912hypothetical protein ORF01957 3913 & 3914 GntR-family transcriptionalregulator ORF01958 3915 & 3916 conserved hypothetical protein ORF019593917 & 3918 fructuronate reductase [1.—.—.—] ORF01960 3919 & 3920metabolite-proton symporter (PPII) ORF01961 3921 & 3922 L-iditol2-dehydrogenase ORF01962 3923 & 3924 Starvation sensing protein rspAORF01963 3925 & 3926 Uncharacterized BCR, YnfA-UPF0060 familysuperfamily ORF01964 3927 & 3928 Protein of unknown function (DUF1283)superfamily ORF01965 3929 & 3930 Spermidine N(1)-acetyltransferase (SAT)[2.3.1.57] ORF01966 3931 & 3932 ynfC protein ORF01967 3933 & 3934Protein of unknown function (DUF1161 ) family ORF01968 3935 & 3936 DmsA[1.8.99.—] ORF01969 3937 & 3938 DmsA [1.8.99.—] ORF01970 3939 & 3940dimethylsulfoxide reductase chain A2 precursor, anaerobic [1.8.—.—]ORF01971 3941 & 3942 Anaerobic dimethyl sulfoxide reductase chain B(dmsB) [1.8.—.—] ORF01972 3943 & 3944 DMSO reductase anchor subunit(DmsC) (dmsC) [1.8.99.—] ORF01973 3945 & 3946 TorD protein ORF01974 3947& 3948 dethiobiotin synthetase (DTBS) ORF01975 3949 & 3950 Putativechloride channel protein eriC-like ORF01976 3951 & 3952 Mlc proteinORF01977 3953 & 3954 transcriptional regulator, LysR family ORF019783955 & 3956 unnamed protein product ORF01979 3957 & 3958 Acid shockprotein repeat family ORF01980 3959 & 3960 glutamyl endopeptidase[3.4.21.—] ORF01981 3961 & 3962 possible chaperone ORF01982 3963 & 3964DMT Superfamily drug efflux pump ORF01983 3965 & 3966 Domain of unknownfunction, putative ORF01984 3967 & 3968 NAD(P) transhydrogenase, betasubunit (pntB) [1.6.1.2] ORF01985 3969 & 3970 NAD(P) transhydrogenase,alpha subunit (pntA) [1.6.1.1] ORF01986 3971 & 3972 Protein ydgHprecursor ORF01987 3973 & 3974 hypothetical protein ORF01988 3975 & 3976unnamed protein product ORF01989 3977 & 3978 fabG5, putative [1.—.—.—]ORF01990 3979 & 3980 Membrane protein glpM ORF01991 3981 & 3982Transcriptional Regulatory protein rstA ORF01992 3983 & 3984 Sensorprotein rstB [2.7.3.—] ORF01993 3985 & 3986 DNA replication terminussite-binding protein ORF01994 3987 & 3988 fumarate hydratase, class II(fumC) [4.2.1.2] ORF01995 3989 & 3990 fumarate hydratase class I,anaerobic [4.2.1.2] ORF01996 3991 & 3992 mannose-6-phosphate isomerase,class I (manA) [5.3.1.8] ORF01997 3993 & 3994 Bacterial protein ofunknown function (DUF945) superfamily ORF01998 3995 & 3996membrane-associated protein ORF01999 3997 & 3998 Glucuronide carrierprotein ORF02000 3999 & 4000 Beta-glucuronidase (GUS) [3.2.1.31]ORF02001 4001 & 4002 transcriptional regulator, TetR family domainprotein, putative ORF02002 4003 & 4004 7-alpha-hydroxysteroiddehydrogenase ORF02003 4005 & 4006 Maltose regulon Regulatory proteinmalI ORF02004 4007 & 4008 PTS system, maltose and glucose-specific IIBCcomponent (malX) [2.7.1.69] ORF02005 4009 & 4010 aminotransferase, classII, putative [4.4.1.8] ORF02006 4011 & 4012 adenosine deaminase (add)[3.5.4.4] ORF02007 4013 & 4014 predicted dehydrogenase ORF02008 4015 &4016 Hha protein ORF02009 4017 & 4018 conserved hypothetical proteinORF02010 4019 & 4020 Electron transport complex protein rnfA ORF020114021 & 4022 Electron transport complex protein rnfB ORF02012 4023 & 4024Electron transport complex protein rnfC ORF02013 4025 & 4026 Electrontransport complex protein rnfD ORF02014 4027 & 4028 Electron transportcomplex protein rnfG (rnfG) ORF02015 4029 & 4030 Electron transportcomplex protein rnfE ORF02016 4031 & 4032 endonuclease III (nth)[4.2.99.18] ORF02017 4033 & 4034 Hypothetical transporter ydgR ORF020184035 & 4036 Glutathione S-transferase [2.5.1.18] ORF02019 4037 & 4038pyridoxal kinase [2.7.1.35] ORF02020 4039 & 4040 tyrosyl-tRNA synthetase(tyrS) [6.1.1.1] ORF02021 4041 & 4042 pyridoxamine 5′-phosphate oxidase(pdxH) [1.4.3.5] ORF02022 4043 & 4044 ydhA protein ORF02023 4045 & 4046Hypothetical UPF0075 protein ydhH ORF02024 4047 & 4048 SlyA ORF020254049 & 4050 Fusaric acid resistance protein FusE. ORF02026 4051 & 4052Fusaric acid resistance protein conserved region family ORF02027 4053 &4054 Copper-zinc superoxide dismutase (sodC) ORF02028 4055 & 4056oxidoreductase, aldo-keto reductase family [1.—.—.—] ORF02029 4057 &4058 unnamed protein product ORF02030 4059 & 4060 transcriptionalregulatory protein ORF02031 4061 & 4062 N-ethylmaleimide reductase(Y13942) [1.—.—.—] ORF02032 4063 & 4064 lactoylglutathione lyase (gloA)[4.4.1.5] ORF02033 4065 & 4066 ribonuclease T (rnt) [3.1.13.—] ORF020344067 & 4068 glutaredoxin-related protein ORF02035 4069 & 4070hypothetical protein ORF02036 4071 & 4072 putative lipoprotein ORF020374073 & 4074 superoxide dismutase, iron [1.15.1.1] ORF02038 4075 & 4076major facilitator family transporter (AL136500) ORF02039 4077 & 4078hypothetical protein ORF02040 4079 & 4080 Purine nucleotide synthesisrepressor (purR) ORF02041 4081 & 4082 cyn operon transcriptionalactivator. ORF02042 4083 & 4084 Bicyclomycin resistance protein(Sulfonamide resistance protein). ORF02043 4085 & 4086cyclopropane-fatty-acyl-phospholipid synthase [2.1.1.79] ORF02044 4087 &4088 riboflavin synthase, alpha subunit (ribE) [2.5.1.9] ORF02045 4089 &4090 Multidrug resistance protein norM ORF02046 4091 & 4092 possibleenzyme ORF02047 4093 & 4094 Protein ydhR precursor ORF02048 4095 & 4096conserved hypothetical protein ORF02049 4097 & 4098 conservedhypothetical protein ORF02050 4099 & 4100 PhsC protein homolog [2.8.1.5]ORF02051 4101 & 4102 nrfC protein homolog b1671 (nrfC) [2.8.1.5]ORF02052 4103 & 4104 conserved hypothetical protein ORF02053 4105 & 4106aldehyde-ferredoxin oxidoreductase PH0892 (aor) [1.—.—.—] ORF02054 4107& 4108 aldehyde oxidoreductase (aor) [1.—.—.—] ORF02055 4109 & 4110iron-sulfur cluster-binding protein [2.8.1.5] ORF02056 4111 & 4112conserved hypothetical protein ORF02057 4113 & 4114 pyruvate kinase(pyk) [2.7.1.40] ORF02058 4115 & 4116 major outer membrane lipoproteinORF02059 4117 & 4118 ErfK-YbiS-YcfS-YnhG family ORF02060 4119 & 4120SufE protein (orf5) ORF02061 4121 & 4122 aminotransferase, class-V[4.4.1.16] ORF02062 4123 & 4124 FeS assembly protein SufD (sufD)ORF02063 4125 & 4126 FeS assembly ATPase SufC (sufC) ORF02064 4127 &4128 FeS assembly protein SufB (sufB) ORF02065 4129 & 4130 FeS assemblyscaffold SufA (sufA) ORF02066 4131 & 4132 unnamed protein productORF02067 4133 & 4134 uncharacterized domain 1, putative ORF02068 4135 &4136 oxidoreductase, FAD-binding, putative ORF02069 4137 & 4138Hypothetical UPF0118 protein ydiK ORF02070 4139 & 4140 conservedhypothetical protein ORF02071 4141 & 4142 transport system permeaseprotein homolog lin2340 ORF02072 4143 & 4144 Hypothetical transportprotein ydiN ORF02073 4145 & 4146 shikimate 5-dehydrogenase [1.1.1.—]ORF02074 4147 & 4148 3-dehydroquinate dehydratase, type I (aroD)[4.2.1.10] ORF02075 4149 & 4150 acetyl-CoA-transferase subunit, putative[2.8.3.—] ORF02076 4151 & 4152 acyl-CoA dehydrogenase family protein,putative [1.3.99.—] ORF02077 4153 & 4154 Hypothetical transcriptionalregulator ydiP ORF02078 4155 & 4156 FixA protein ORF02079 4157 & 4158electron transfer flavoprotein, alpha subunit, putative ORF02080 4159 &4160 flavoprotein, probably electron transport [1.5.5.—] ORF02081 4161 &4162 Ferredoxin-like protein ydiT ORF02082 4163 & 4164 substrate--CoAligase, putative [6.2.1.—] ORF02083 4165 & 4166 phosphoenolpyruvatesynthase (ppsA) [2.7.9.2] ORF02084 4167 & 4168 Hypothetical UPF0085protein ydiA ORF02085 4169 & 4170 phospho-2-dehydro-3-deoxyheptonatealdolase [2.5.1.54] ORF02086 4171 & 4172 conserved hypothetical proteinORF02087 4173 & 4174 Hypothetical UPF0061 protein ydiU (AP001520)ORF02088 4175 & 4176 conserved hypothetical protein ORF02089 4177 & 4178lipoprotein (ORF) ORF02090 4179 & 4180 Vitamin B12 transport ATP-bindingprotein btuD (VitaminB12-transporting ATPase) [3.6.3.33] ORF02091 4181 &4182 Glutathione peroxidase ORF02092 4183 & 4184 Vitamin B12 transportsystem permease protein btuC (membrane) ORF02093 4185 & 4186 integrationhost factor, alpha subunit (ihfA) ORF02094 4187 & 4188 phenylalanyl-tRNAsynthetase, beta subunit (pheT) [6.1.1.20] ORF02095 4189 & 4190phenylalanyl-tRNA synthetase, alpha subunit (pheS) [6.1.1.20] ORF020964191 & 4192 ribosomal protein L20 (rplT) ORF02097 4193 & 4194 ribosomalprotein L35 (rpmI) ORF02098 4195 & 4196 translation initiation factorIF-3 (infC) ORF02099 4197 & 4198 threonyl-tRNA synthetase (thrS)[6.1.1.3] ORF02100 4199 & 4200 InsAB′ protein ORF02101 4201 & 4202 InsAprotein ORF02102 4203 & 4204 hypothetical protein ORF02103 4205 & 4206Protein of unknown function, DUF481 superfamily ORF02104 4207 & 4208pfkB (pfkB) [2.7.1.11] ORF02105 4209 & 4210 conserved hypotheticalprotein ORF02106 4211 & 4212 Fructosamine kinase subfamily ORF02107 4213& 4214 conserved hypothetical protein ORF02108 4215 & 4216 Protein yniC(YniC) [3.1.3.18] ORF02109 4217 & 4218 Predicted membrane-boundmetal-dependent hydrolase (DUF457) family ORF02110 4219 & 4220 sodium:dicarboxylate symporter family protein ORF02111 4221 & 4222 Celldivision activator cedA ORF02112 4223 & 4224 conserved hypotheticalprotein ORF02113 4225 & 4226 Catalase HPII (III) [1.11.1.6] ORF021144227 & 4228 unnamed protein product; ORF 28.5 ORF02115 4229 & 42306-phospho-beta-glucosidase [3.2.1.86] ORF02116 4231 & 4232 Cel operonrepressor ORF02117 4233 & 4234 PEP-dependent phosphotransferase enzymeIII for cellobiose, arbutin, and salicin (PTS) [2.7.1.69] ORF02118 4235& 4236 PTS system, cellobiose-specific IIC component (celB) ORF021194237 & 4238 PTS system, N,N′-diacetylchitobiose-specific IIB component(EIIB-Chb)(N,N′- diacetylchitobiose-permease IIB component)(Phosphotransferaseenzyme II, B component) [2.7.1.69] ORF02120 4239 &4240 Osmotically inducible lipoprotein E precursor ORF02121 4241 & 4242NAD+ synthetase (nadE) [6.3.1.5] ORF02122 4243 & 4244 Bacterial proteinof unknown function (DUF886) superfamily ORF02123 4245 & 4246excinuclease ABC subunit C homolog ORF02124 4247 & 4248 Spheroplastprotein Y precursor ORF02125 4249 & 4250 conserved hypothetical proteinORF02126 4251 & 4252 Succinylglutamate desuccinylase [3.1.—.—] ORF021274253 & 4254 Succinylarginine dihydrolase (astB) ORF02128 4255 & 4256Succinate semialdehyde dehydrogenase (EC 1.2.1.24) (NAD(+)-dependentsuccinic semialdehyde dehydrogenase). (retinol) [1.2.1.24] ORF02129 4257& 4258 Arginine N-succinyltransferase beta subunit (astA) [2.3.1.109]ORF02130 4259 & 4260 Succinylornithine transaminase(Succinylornithineaminotransferase) (ACOAT) [2.6.1.—] ORF02131 4261 &4262 exodeoxyribonuclease III (xth) [3.1.11.2] ORF02132 4263 & 4264membrane protein, probable, putative ORF02133 4265 & 4266 conservedhypothetical protein ORF02134 4267 & 4268 DedA family family ORF021354269 & 4270 IS110 family transposase, truncation, putative ORF02136 4271& 4272 Protein ynjB ORF02137 4273 & 4274 Hypothetical ABC transporterpermease protein ynjC ORF02138 4275 & 4276 Inner membrane protein MalK(P-loop) ORF02139 4277 & 4278 2.8.1.1 [2.8.1.1] ORF02140 4279 & 4280phosphatidylglycerophosphate synthase ORF02141 4281 & 4282 Protein ofunknown function (DUF1496) superfamily ORF02142 4283 & 4284 mutT2[3.6.1.—] ORF02143 4285 & 4286 NADP-specific glutamate dehydrogenase(NADP-GDH) [1.4.1.4] ORF02144 4287 & 4288 DNA topoisomerase III ORF021454289 & 4290 selenide, water dikinase (selD) [2.7.9.3] ORF02146 4291 &4292 Protein ydjA ORF02147 4293 & 4294 hypothetical protein ORF021484295 & 4296 signal peptide peptidase SppA, 67K type (sppA) [3.4.—.—]ORF02149 4297 & 4298 L-asparaginase (ansA) [3.5.1.1] ORF02150 4299 &4300 Pyrazinamidase-nicotinamidase (PZASE) [3.5.1.—] ORF02151 4301 &4302 putative transport protein ORF02152 4303 & 4304 similar totranscription regulator DeoR family ORF02153 4305 & 4306 aldo-ketoreductase Atu3877 (gsp69) [1.—.—.—] ORF02154 4307 & 4308 putative kinaseORF02155 4309 & 4310 Fructose-tagatose bisphosphate aldolase [4.1.2.—]ORF02156 4311 & 4312 Sorbitol dehydrogenase (EC 1.1.1.14) (L-iditol2-dehydrogenase). (sorbitol) [1.1.1.14] ORF02157 4313 & 4314 Membranetransporter D1. ORF02158 4315 & 4316 sorbitol dehydrogenase, putative[1.1.1.—] ORF02159 4317 & 4318 Protein of unknown function (DUF1315)superfamily ORF02160 4319 & 4320 methionine-R-sulfoxide reductase (msrB)[1.8.4.6] ORF02161 4321 & 4322 glyceraldehyde-3-phosphate dehydrogenase,type I (gap) [1.2.1.—] ORF02162 4323 & 4324 transglycolase (fragment)ORF02163 4325 & 4326 Morphine 6-dehydrogenase (EC 1.1.1.218) (Naloxonereductase). (PA0804) [1.1.1.—] ORF02164 4327 & 4328 MltA-interactingprotein precursor ORF02165 4329 & 4330 hypothetical protein ORF021664331 & 4332 protein kinase ORF02167 4333 & 4334 Hypothetical UPF0229protein yeaH ORF02168 4335 & 4336 GGDEF domain protein ORF02169 4337 &4338 GGDEF domain protein ORF02170 4339 & 4340 unnamed protein productORF02171 4341 & 4342 transcriptional regulator araC family ORF02172 4343& 4344 Protein of unknown function (DUF441) superfamily ORF02173 4345 &4346 cyanate MFS transporter (permease) ORF02174 4347 & 4348 Protein ofunknown function, DUF488 superfamily ORF02175 4349 & 4350 hemolysinVCA0594 ORF02176 4351 & 4352 GGDEF domain protein ORF02177 4353 & 4354hypothetical protein ORF02178 4355 & 4356 Transglycosylase associatedprotein ORF02179 4357 & 4358 conserved hypothetical protein ORF021804359 & 4360 tellurite resistance protein TehB, putative ORF02181 4361 &4362 hypothetical protein ORF02182 4363 & 4364 translocator protein,LysE family superfamily ORF02183 4365 & 4366 conserved hypotheticalprotein ORF02184 4367 & 4368 tartrate dehydrogenase (3-IPM-DH)[1.1.1.93] ORF02185 4369 & 4370 ribonuclease D (rnd) [3.1.26.3] ORF021864371 & 4372 Long-chain-fatty-acid--CoA ligase ORF02187 4373 & 4374 outermembrane lipoprotein ORF02188 4375 & 4376 Hypothetical M22 peptidasehomolog yeaZ [3.4.24.57] ORF02189 4377 & 4378 unnamed protein productORF02190 4379 & 4380 Endoribonuclease L-PSP superfamily ORF02191 4381 &4382 conserved hypothetical protein ORF02192 4383 & 4384 HypotheticalUPF0181 protein yoaH-related protein ORF02193 4385 & 4386Para-aminobenzoate synthase component I [6.3.5.8] ORF02194 4387 & 4388MutT-family protein ORF02195 4389 & 4390 L-serine ammonia-lyase (sdaA)[4.3.1.17] ORF02196 4391 & 4392 EAL domain protein ORF02197 4393 & 4394possible CorC-HlyC family of Mg+2-Co+2-heavy metal efflux pumps ORF021984395 & 4396 PTS enzyme IIAB, mannose-specific (EIII-MAN) ORF02199 4397 &4398 PTS system, mannose-specific IIC component ORF02200 4399 & 4400 PTSsystem, mannose-specific IID component ORF02201 4401 & 4402 HypotheticalUPF0266 protein yobD ORF02202 4403 & 4404 Domain of unknown function DUFfamily ORF02203 4405 & 4406 23S rRNA m1G745 methyltransferase, putative[2.1.1.51] ORF02204 4407 & 4408 Cold shock-like protein cspE ORF022054409 & 4410 conserved hypothetical protein ORF02206 4411 & 4412lipoprotein, putative ORF02207 4413 & 4414 conserved hypotheticalprotein ORF02208 4415 & 4416 Transcriptional regulator kdgR ORF022094417 & 4418 MULTIDRUG RESISTANCE PROTEIN B ORF02210 4419 & 4420 heatshock protein, integral membrane protein (htpX) [3.4.24.—] ORF02211 4421& 4422 Tail-specific protease precursor [3.4.21.102] ORF02212 4423 &4424 carboxy-terminal protease for penicillin-binding protein 3 ORF022134425 & 4426 ProP effector ORF02214 4427 & 4428 GAF domain proteinORF02215 4429 & 4430 PqiA protein ORF02216 4431 & 4432 PqiA proteinORF02217 4433 & 4434 mce related protein family ORF02218 4435 & 4436Sun-nucleolar protein family protein ORF02219 4437 & 4438 Protein ofunknown function (DUF1480) superfamily ORF02220 4439 & 4440 Protein ofunknown function (DUF1482) family ORF02221 4441 & 4442 Serine-threonineprotein phosphatase 1 ORF02222 4443 & 4444 conserved hypotheticalprotein ORF02223 4445 & 4446 unnamed protein product ORF02224 4447 &4448 Copper resistance protein D family, putative ORF02225 4449 & 4450Protein yobA precursor ORF02226 4451 & 4452 DNA polymerase III, thetasubunit [2.7.7.7] ORF02227 4453 & 4454 hydrolase, carbon-nitrogen familyfamily ORF02228 4455 & 4456 DNA polymerase III, alpha subunit,Gram-positive type, putative ORF02229 4457 & 4458 protease II (AB004795)[3.4.21.83] ORF02230 4459 & 4460 unidentified reading frame ORF022314461 & 4462 unnamed protein product; Similar to lipoprotein YebFprecursor of Escherichia coli ORF02232 4463 & 4464 hypothetical proteinORF02233 4465 & 4466 DNA damage-inducible gene in SOS regulon, dependenton cyclic AMP and H—NS ORF02234 4467 & 4468 phosphoribosylglycinamideformyltransferase 2 (purT) [2.1.2.—] ORF02235 4469 & 44702-deydro-3-deoxyphosphogluconate aldolase-4-hydroxy-2-oxoglutaratealdolase (eda) [4.1.3.16] ORF02236 4471 & 4472 phosphogluconatedehydratase (edd) [4.2.1.12] ORF02237 4473 & 4474 glucose-6-phosphate1-dehydrogenase (zwf) [1.1.1.49] ORF02238 4475 & 4476 conservedhypothetical protein ORF02239 4477 & 4478 Hex regulon repressor ORF022404479 & 4480 pyruvate kinase (pyk) [2.7.1.40] ORF02241 4481 & 4482 lipidA biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (msbB)[2.3.1.—] ORF02242 4483 & 4484 Cell envelope ORF02243 4485 & 4486High-affinity zinc uptake system protein znuA precursor (AE005408)ORF02244 4487 & 4488 ABC superfamily high affinity Zn transport protein(atp_bind) ORF02245 4489 & 4490 High-affinity zinc uptake systemmembrane protein znuB (atp_bind) ORF02246 4491 & 4492 Holliday junctionDNA helicase RuvB (ruvB) ORF02247 4493 & 4494 Holliday junction DNAhelicase RuvA (ruvA) ORF02248 4495 & 4496 Protein of unknown function(DUF1105) superfamily ORF02249 4497 & 4498 crossover junctionendodeoxyribonuclease RuvC (ruvC) [3.1.22.4] ORF02250 4499 & 4500conserved hypothetical protein TIGR01033 ORF02251 4501 & 4502 dATPpyrophosphohydrolase ORF02252 4503 & 4504 aspartyl-tRNA synthetase(aspS) [6.1.1.12] ORF02253 4505 & 4506 Hypothetical isochorismatasefamily protein yecD ORF02254 4507 & 4508 Protein of unknown functionsuperfamily ORF02255 4509 & 4510 conserved hypothetical protein ORF022564511 & 4512 methyltransferase, putative ORF02257 4513 & 4514methyltransferase, putative ORF02258 4515 & 4516 Trimethylamine-N-oxidereductase 2 precursor ORF02259 4517 & 4518 Cytochrome c-type proteintorY ORF02260 4519 & 4520 copper homeostasis protein VC0730 ORF022614521 & 4522 Protein yecM ORF02262 4523 & 4524 arginyl-tRNA synthetase(argS) [6.1.1.19] ORF02263 4525 & 4526 Flagellar protein flhE precursorORF02264 4527 & 4528 flagellar biosynthesis protein FlhA (flhA) ORF022654529 & 4530 flagellar biosynthetic protein FlhB (flhB) ORF02266 4531 &4532 conserved hypothetical protein ORF02267 4533 & 4534 Chemotaxisprotein cheZ ORF02268 4535 & 4536 Chemotaxis protein cheY ORF02269 4537& 4538 Chemotaxis response regulator protein-glutamate methylesterase(EC3.1.1.61) [3.1.1.61] ORF02270 4539 & 4540 Chemotaxis proteinmethyltransferase [2.1.1.80] ORF02271 4541 & 4542 methyl-acceptingChemotaxis protein IV, peptide sensor receptor ORF02272 4543 & 4544conserved hypothetical protein ORF02273 4545 & 4546 Methyl-acceptingChemotaxis protein II ORF02274 4547 & 4548 Chemotaxis protein cheWORF02275 4549 & 4550 Chemotaxis protein cheA ORF02276 4551 & 4552Chemotaxis motB protein ORF02277 4553 & 4554 Chemotaxis motA proteinORF02278 4555 & 4556 Flagellar transcriptional activator flhC ORF022794557 & 4558 regulator of flagellar biosynthesis, acting on class 2operons; transcriptional initiation factor ORF02280 4559 & 4560Universal stress protein C (UspA) ORF02281 4561 & 4562alpha,alpha-trehalose-phosphate synthase [UDP-forming] (otsA) [2.4.1.15]ORF02282 4563 & 4564 trehalose-phosphatase (otsB) [3.1.3.12] ORF022834565 & 4566 L-arabinose transport system permease protein araH ORF022844567 & 4568 L-arabinose transport ATP-binding protein araG ORF02285 4569& 4570 L-arabinose-binding periplasmic protein precursor (PBP) ORF022864571 & 4572 Ferritin-like protein 2 ORF02287 4573 & 4574 conservedhypothetical protein ORF02288 4575 & 4576 lipoprotein, putative ORF022894577 & 4578 conserved hypothetical protein ORF02290 4579 & 4580cytoplasmic ferritin (an iron storage protein) ORF02291 4581 & 4582conserved hypothetical protein ORF02292 4583 & 4584 Tyrosine-specifictransport protein ORF02293 4585 & 4586 preprotein translocase, SecAsubunit ORF02294 4587 & 4588 conserved hypothetical protein ORF022954589 & 4590 Fels-2 prophage: similar to retron in E ORF02296 4591 & 4592ParA ORF02297 4593 & 4594 Sb32 ORF02298 4595 & 4596 hypothetical proteinORF02299 4597 & 4598 phage portal protein, PBSX family ORF02300 4599 &4600 Putative ATPase subunit of terminase (gpP-like) ORF02301 4601 &4602 Phage capsid scaffolding protein (GPO) ORF02302 4603 & 4604 phagemajor capsid protein, P2 family ORF02303 4605 & 4606 Phage smallterminase subunit ORF02304 4607 & 4608 head completion-stabilizationprotein L ORF02305 4609 & 4610 Phage Tail Protein X ORF02306 4611 & 4612Bbp2 ORF02307 4613 & 4614 conserved hypothetical protein ORF02308 4615 &4616 phage tail completion protein R ORF02309 4617 & 4618 Fels-2prophage: similar to gpS for completion ORF02310 4619 & 4620 baseplateassembly protein V (gpV) ORF02311 4621 & 4622 phage baseplate assemblyprotein W (gpW) ORF02312 4623 & 4624 Baseplate J-like protein ORF023134625 & 4626 gpI (gpI) ORF02314 4627 & 4628 gpH (gpH) ORF02315 4629 &4630 gpU prime (gpG) ORF02316 4631 & 4632CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase(pgsA) [2.7.8.5] ORF02317 4633 & 4634 excinuclease ABC, C subunit (uvrC)ORF02318 4635 & 4636 response regulator protein ORF02319 4637 & 4638conserved hypothetical protein ORF02320 4639 & 4640 Regulatory proteinsdiA ORF02321 4641 & 4642 amino acid ABC transporter, ATP-bindingprotein ORF02322 4643 & 4644 amino acid ABC transporter, permeaseprotein (permease) ORF02323 4645 & 4646 4.4.1.15 [3.5.99.7] ORF023244647 & 4648 Cystine-binding periplasmic protein precursor ORF02325 4649& 4650 FliZ protein ORF02326 4651 & 4652 transcription initiation factoralternative sigma factor 28 (Sigma) ORF02327 4653 & 4654 FliC ORF023284655 & 4656 Flagellar hook-associated protein 2 (HAP2) ORF02329 4657 &4658 flagellar protein FliS (fliS) ORF02330 4659 & 4660 flagellarprotein FliT ORF02331 4661 & 4662 Cytoplasmic alpha-amylase [3.2.1.1]ORF02332 4663 & 4664 Hypothetical lipoprotein yedD precursor ORF023334665 & 4666 YeeE-YedE family protein family ORF02334 4667 & 4668Hypothetical UPF0033 protein yedF-related protein ORF02335 4669 & 4670Uncharacterized ACR, COG2135 superfamily ORF02336 4671 & 4672 Outermembrane porin protein nmpC precursor ORF02337 4673 & 4674 Hypotheticaltranscriptional regulator ybcM ORF02338 4675 & 4676 conservedhypothetical protein ORF02339 4677 & 4678 EmrE protein ORF02340 4679 &4680 flagellar hook-basal body complex protein (FliE) (fliE) ORF023414681 & 4682 flagellar M-ring protein FliF (fliF) ORF02342 4683 & 4684flagellar motor switch protein FliG (fliG) ORF02343 4685 & 4686Flagellar assembly protein FliH (fliH) ORF02344 4687 & 4688flagellum-specific ATP synthase ORF02345 4689 & 4690 flagellar exportprotein FliJ (fliJ) ORF02346 4691 & 4692 flagellar hook-length controlprotein ORF02347 4693 & 4694 Flagellar basal body-associated proteinFliL (fliL) ORF02348 4695 & 4696 flagellar motor switch protein FliM(fliM) ORF02349 4697 & 4698 Flagellar motor switch protein fliN ORF023504699 & 4700 Flagellar protein fliO ORF02351 4701 & 4702 Unknown proteinencoded by cryptic prophage ORF02352 4703 & 4704 Helix-turn-helix domainprotein ORF02353 4705 & 4706 stability determinant ORF02354 4707 & 4708unknown protein encoded by prophage CP-933N -related protein ORF023554709 & 4710 Division inhibition protein dicB ORF02356 4711 & 4712conserved hypothetical protein ORF02357 4713 & 4714 ExodeoxyribonucleaseVIII (35kD) [3.1.11.—] ORF02358 4715 & 4716 conserved hypotheticalprotein ORF02359 4717 & 4718 Sb28 (AE005423) ORF02360 4719 & 4720Protein of unknown function (DUF980) superfamily ORF02361 4721 & 4722hypothetical protein ORF02362 4723 & 4724 integrase ORF02363 4725 & 4726trpE2 ORF02364 4727 & 4728 Putative cytoplasmic transmembrane proteinORF02365 4729 & 4730 ABC transporter, ATP-binding-permease protein,putative ORF02366 4731 & 4732 EF0101 ORF02367 4733 & 4734 hypotheticalprotein ORF02368 4735 & 4736 Putataive AraC type regulator ORF02369 4737& 4738 dihydroaeruginoic acid synthetase ORF02370 4739 & 4740yersiniabactin biosynthetic protein ORF02371 4741 & 4742 yersiniabactinbiosynthetic protein YbtU ORF02372 4743 & 4744 yersiniabactinbiosynthetic protein YbtT ORF02373 4745 & 4746 mbtA [6.2.1.—] ORF023744747 & 4748 OMR family pesticin-yersiniabactin receptor protein (IRPC)ORF02375 4749 & 4750 conserved hypothetical protein ORF02376 4751 & 4752putative factor ORF02377 4753 & 4754 putative factor ORF02378 4755 &4756 conserved hypothetical protein ORF02379 4757 & 4758 majorfacilitator family transporter (PPII) ORF02380 4759 & 4760 AMPnucleosidase (amn) [3.2.2.4] ORF02381 4761 & 4762 Domain of unknownfunction, putative ORF02382 4763 & 4764 MelD protein ORF02383 4765 &4766 amidase family protein, putative ORF02384 4767 & 4768 MATE effluxfamily protein subfamily ORF02385 4769 & 4770 Putative peptidesynthetase ORF02386 4771 & 4772 NosB (AF183408) ORF02387 4773 & 4774fmtA-like protein, putative ORF02388 4775 & 4776 thioesterase ORF023894777 & 4778 Prophage P4 integrase ORF02390 4779 & 4780 hypotheticalprotein ORF02391 4781 & 4782 MATE efflux family protein subfamilyORF02392 4783 & 4784 TubD protein [6.3.2.—] ORF02393 4785 & 4786 BarGORF02394 4787 & 4788 NosB ORF02395 4789 & 4790 AMP-binding enzyme domainprotein ORF02396 4791 & 4792 [acyl-carrier-protein] S-malonyltransferase[2.3.1.39] ORF02397 4793 & 4794 Putative acyl-coa dehydrogenase ORF023984795 & 4796 acyl carrier protein ORF02399 4797 & 4798 3-hydroxyacyl CoAdehydrogenase (BHBD) [1.1.1.157] ORF02400 4799 & 4800 JamP ORF02401 4801& 4802 oxidoreductase, zinc-binding dehydrogenase family family ORF024024803 & 4804 possible DNA-binding response regulator ORF02403 4805 & 4806EntD-Gsp-Hetl-Sfp family protein [2.7.8.—] ORF02404 4807 & 4808transposase ORF02405 4809 & 4810 ISSo2, transposase OrfB, truncationORF02406 4811 & 4812 Protein erfK-srfK precursor ORF02407 4813 & 4814Nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase(EC 2.4.2.21) (NN: DBI PRT)(N(1)-alpha-phosphoribosyltransferase) [2.4.2.21] ORF02408 4815 & 4816cobalamin 5′-phosphate synthase (cobS) [2.7.8.26] ORF02409 4817 & 4818Cobalamin biosynthesis protein cobU [2.7.1.156] ORF02410 4819 & 4820conserved hypothetical protein ORF02411 4821 & 4822 conservedhypothetical protein ORF02412 4823 & 4824 TonB dependent receptor,putative ORF02413 4825 & 4826 conserved hypothetical protein ORF024144827 & 4828 Z1204 protein ORF02415 4829 & 4830 conserved hypotheticalprotein ORF02416 4831 & 4832 glucosaminyltransferase ORF02417 4833 &4834 Sugar phosphate nucleotydyl transferase ORF02418 4835 & 4836conserved hypothetical protein ORF02419 4837 & 4838 host specificityprotein J, truncation, putative ORF02420 4839 & 4840 host specificityprotein (partial) ORF02421 4841 & 4842 host specificity protein(partial) ORF02422 4843 & 4844 Terminase small subunit (GP1) (fragment)ORF02423 4845 & 4846 conserved hypothetical protein ORF02424 4847 & 4848Partial tonB-like membrane protein encoded within prophage ORF02425 4849& 4850 hypothetical protein ORF02426 4851 & 4852 Bor protein homologfrom lambdoid prophage DLP12 ORF02427 4853 & 4854 Bacteriophage lysisprotein ORF02428 4855 & 4856 lipoprotein Rz1 precursor -related proteinORF02429 4857 & 4858 hypothetical protein ORF02430 4859 & 4860 Lysozyme(Lysis protein) (Muramidase) (Endolysin) (lysozyme) [3.2.1.17] ORF024314861 & 4862 lysis protein S.b1556 ORF02432 4863 & 4864 hypotheticalprotein ORF02433 4865 & 4866 Outer membrane porin protein nmpC precursor(lbc) ORF02434 4867 & 4868 Antitermination protein Q homolog fromlambdoid prophage ORF02435 4869 & 4870 endodeoxyribonuclease RUS(Holliday junction resolvase) ORF02436 4871 & 4872 Protein ninE-relatedprotein ORF02437 4873 & 4874 phage N-6-adenine-methyltransferase[2.1.1.72] ORF02438 4875 & 4876 Protein ninB ORF02439 4877 & 4878replication protein P ORF02440 4879 & 4880 transposase ORF02441 4881 &4882 ECs1339 ORF02442 4883 & 4884 ribokinase ORF02443 4885 & 4886Protein of unknown function (DUF1355) superfamily ORF02444 4887 & 4888membrane protein, putative ORF02445 4889 & 4890 puativephophotriesterase (PTE) [3.1.8.1] ORF02446 4891 & 4892gamma-glutamyltranspeptidase ORF02447 4893 & 4894gamma-glutamyltranspeptidase ORF02448 4895 & 4896 ST55 protein ORF024494897 & 4898 conserved hypothetical protein ORF02450 4899 & 4900conserved hypothetical protein ORF02451 4901 & 4902 ISBma3, transposase,truncation ORF02452 4903 & 4904 Prophage CP4-57 regulatory protein(AlpA) family ORF02453 4905 & 4906 hypothetical protein ORF02454 4907 &4908 conserved hypothetical protein ORF02455 4909 & 4910 hypotheticalprotein ORF02456 4911 & 4912 Insertion sequence ATP-binding proteinORF02457 4913 & 4914 Transposase ORF02458 4915 & 4916 conservedhypothetical protein ORF02459 4917 & 4918 ferric-enterobactin-transportprotein (III) ORF02460 4919 & 4920 ABC transporter permease STY0802(FecCD_family) ORF02461 4921 & 4922 Periplasmic binding protein (III)ORF02462 4923 & 4924 TonB dependent receptor ORF02463 4925 & 4926 YeePprotein ORF02464 4927 & 4928 conserved hypothetical protein ORF024654929 & 4930 conserved hypothetical protein ORF02466 4931 & 4932conserved hypothetical protein ORF02467 4933 & 4934 Patatin-likephospholipase family ORF02468 4935 & 4936 conserved hypothetical proteinORF02469 4937 & 4938 conserved hypothetical protein ORF02470 4939 & 4940conserved hypothetical protein ORF02471 4941 & 4942 Z1215 protein (o273)ORF02472 4943 & 4944 Antirestriction protein family ORF02473 4945 & 4946YeeS protein (o160) ORF02474 4947 & 4948 Protein of unknown function(DUF987) superfamily ORF02475 4949 & 4950 YeeU protein ORF02476 4951 &4952 L0007-like protein ORF02477 4953 & 4954 YeeW protein ORF02478 4955& 4956 Protein of unknown function (DUF496) superfamily ORF02479 4957 &4958 conserved hypothetical protein ORF02480 4959 & 4960 DNA gyraseinhibitory protein ORF02481 4961 & 4962 Penicillin-binding protein 6Bprecursor (PBP) ORF02482 4963 & 4964 penicillin binding protein 6bORF02483 4965 & 4966 Exodeoxyribonuclease I ORF02484 4967 & 4968 aminoacid permease ORF02485 4969 & 4970 transcriptional regulator, LysRfamily ORF02486 4971 & 4972 Protein yeeZ precursor ORF02487 4973 & 4974addiction module toxin, Txe-YoeB family ORF02488 4975 & 4976 yefMprotein ORF02489 4977 & 4978 ATP phosphoribosyltransferase (hisG)[2.4.2.17] ORF02490 4979 & 4980 histidinol dehydrogenase (hisD)[1.1.1.23] ORF02491 4981 & 4982 histidinol-phosphate aminotransferase(hisC) [2.6.1.9] ORF02492 4983 & 4984 Histidine biosynthesisbifunctional protein hisB ORF02493 4985 & 4986 Imidazole glycerolphosphate synthase subunit hisH (IGPsynthase glutamine amidotransferasesubunit) (IGP synthase subunithisH) (ImGP synthase subunit hisH)(IGPSsubunit hisH) [2.4.2.—] ORF02494 4987 & 4988 imidazole glycerolphosphate synthase, glutamine amidotransferase subunit (hisH) [2.4.2.—]ORF02495 4989 & 4990 phosphoribosylformimino-5-aminoimidazolecarboxamide ribotide isomerase (hisA) [5.3.1.16] ORF02496 4991 & 4992imidazoleglycerol phosphate synthase, cyclase subunit (hisF) ORF024974993 & 4994 Histidine biosynthesis bifunctional protein hisIE [Includes:Phosphoribosyl-AMP cyclohydrolase (PRA-CH); Phosphoribosyl-ATPpyrophosphatase (EC 3.6.1.31) (PRA-PH)] (PRA-PH) [3.5.4.19] ORF024984995 & 4996 Chain length determinant protein ORF02499 4997 & 4998UDP-glucose 6-dehydrogenase (UDPGDH) [1.1.1.22] ORF02500 4999 & 50006-phosphogluconate dehydrogenase, decarboxylating (gnd) [1.1.1.44]ORF02501 5001 & 5002 WbnE [2.4.1.—] ORF02502 5003 & 5004 putativeglycosyltransferase [2.4.1.—] ORF02503 5005 & 5006 glycosyl transferaseCpsO ORF02504 5007 & 5008 rhamnosyl transferase Cps6bS ORF02505 5009 &5010 O-antigen modification protein ORF02506 5011 & 5012 rfbX proteinORF02507 5013 & 5014 dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC)[5.1.3.13] ORF02508 5015 & 5016 glucose-1-phosphatethymidylyltransferase (rfbA) [2.7.7.24] ORF02509 5017 & 5018dTDP-4-dehydrorhamnose reductase (rfbD) [1.1.1.133] ORF02510 5019 & 5020dTDP-glucose 4,6-dehydratase (rfbB) [4.2.1.46] ORF02511 5021 & 5022regulatory protein GalF (galF) ORF02512 5023 & 5024 Colanic acidbiosynthesis protein wcaM ORF02513 5025 & 5026 Amylovoran biosynthesisglycosyl transferase amsK [2.—.—.—] ORF02514 5027 & 5028 Colanic acidbiosynthesis protein wcaK [2.—.—.—] ORF02515 5029 & 5030Lipopolysaccharide biosynthesis protein wzxC ORF02516 5031 & 5032glycosyl transferase (WcaJ) [2.7.—.—] ORF02517 5033 & 5034Phosphomannomutase ORF02518 5035 & 5036 mannose-1-phosphateguanylyltransferase-mannose-6-phosphate isomerase ORF02519 5037 & 5038lipopolysaccharide biosynthesis protein, putative ORF02520 5039 & 5040GDP-mannose mannosyl hydrolase [3.6.1.—] ORF02521 5041 & 5042 GDP-fucosesynthetase chain A (GER1) [1.1.1.271] ORF02522 5043 & 5044 GDP-mannose4,6-dehydratase (gmd) [4.2.1.47] ORF02523 5045 & 5046 acetyltransferase,CysE-LacA-LpxA-NodL family, putative [2.3.1.—] ORF02524 5047 & 5048glycosyl transferase, group 2 family protein, putative ORF02525 5049 &5050 Putative colanic acid polymerase ORF02526 5051 & 5052 possibleglycosyl transferase ORF02527 5053 & 5054 serine acetyltransferase[2.3.1.—] ORF02528 5055 & 5056 possible glycosyl transferase ORF025295057 & 5058 Tyrosine-protein kinase wzc ORF02530 5059 & 5060 Lowmolecular weight protein-tyrosine-phosphatase wzb [3.1.3.48] ORF025315061 & 5062 membrane protein ORF02532 5063 & 5064 putative transportprotein ORF02533 5065 & 5066 unnamed protein product ORF02534 5067 &5068 PHIKZ114 ORF02535 5069 & 5070 Protein asmA precursor ORF02536 5071& 5072 deoxycytidine triphosphate deaminase (dcd) [3.5.4.13] ORF025375073 & 5074 uridine kinase (udk) [2.7.1.48] ORF02538 5075 & 5076 MASE1domain family ORF02539 5077 & 5078 Ada regulatory protein, putative[3.2.2.21] ORF02540 5079 & 5080 hypothetical protein ORF02541 5081 &5082 Molecular chaperone ORF02542 5083 & 5084 conserved hypotheticalprotein ORF02543 5085 & 5086 conserved hypothetical protein ORF025445087 & 5088 von Willebrand factor type A domain protein ORF02545 5089 &5090 putative membrane protein ORF02546 5091 & 5092 AcrB-AcrD-AcrF(HAE1) ORF02547 5093 & 5094 AcrB-AcrD-AcrF family membrane proteinORF02548 5095 & 5096 MFS superfamily drug efflux pump ORF02549 5097 &5098 sensor histidine kinase SrrB, putative [2.7.3.—] ORF02550 5099 &5100 Transcriptional regulatory protein baeR (ResD) ORF02551 5101 & 5102Domain of unknown function (DUF1508) family ORF02552 5103 & 5104conserved hypothetical protein ORF02553 5105 & 5106 conservedhypothetical protein ORF02554 5107 & 5108 conserved hypothetical proteinORF02555 5109 & 5110 conserved hypothetical protein ORF02556 5111 & 5112YegQ [3.4.—.—] ORF02557 5113 & 5114 conserved hypothetical proteinTIGR00147 ORF02558 5115 & 5116 Galactitol utilization operon repressor(fragment) ORF02559 5117 & 5118 hexitol dehydrogenase [1.1.1.251]ORF02560 5119 & 5120 PTS system, sorbitol-specific IIC component(EIIC-GAT) ORF02561 5121 & 5122 PTS system, galactitol-specific IIBcomponent (EIIB-Gat) (Galacticol-permease IIB component)(Phosphotransferase enzyme II, B component)(EC 2.7.1.69) [2.7.1.69]ORF02562 5123 & 5124 PTS system, galactitol-specific IIA component(EIIA-Gat) (Galacticol-permease IIA component) (Phosphotransferaseenzyme II, A component)(EC 2.7.1.69) (EIIA- GAT) [2.7.1.69] ORF025635125 & 5126 PUTATIVE TAGATOSE 6-PHOSPHATE KINASE PROTEIN [2.7.1.144]ORF02564 5127 & 5128 class II aldolase, tagatose bisphosphate family[4.1.2.—] ORF02565 5129 & 5130 dehydrin [4.1.2.13] ORF02566 5131 & 5132nucleoside permease NupG, putative ORF02567 5133 & 5134ADP-ribosylglycohydrolase, putative ORF02568 5135 & 5136 transcriptionalregulator, GntR family, putative ORF02569 5137 & 5138 Ribokinase (EC2.7.1.15). [2.7.1.15] ORF02570 5139 & 5140 glycosyl hydrolase, family 25[3.2.1.17] ORF02571 5141 & 5142 phosphomethylpyrimidine kinase (thiD)[2.7.4.7] ORF02572 5143 & 5144 hydroxyethylthiazole kinase (thiM)[2.7.1.50] ORF02573 5145 & 5146 Uncharacterized BCR, COG1937 familyORF02574 5147 & 5148 NirC ORF02575 5149 & 5150 conserved hypotheticalprotein ORF02576 5151 & 5152 conserved hypothetical protein ORF025775153 & 5154 Hypothetical outer membrane usher protein yehB precursorORF02578 5155 & 5156 Hypothetical fimbrial chaperone yehC precursorORF02579 5157 & 5158 Fimbrial protein subfamily ORF02580 5159 & 5160 Mrpprotein (mrp) ORF02581 5161 & 5162 Methionyl-tRNA synthetase (metG)[6.1.1.10] ORF02582 5163 & 5164 molybdate metabolism regulator, secondfragment 2 (fragment) ORF02583 5165 & 5166 yehI (fragment) ORF02584 5167& 5168 yehI (fragment) ORF02585 5169 & 5170 conserved hypotheticalprotein ORF02586 5171 & 5172 conserved hypothetical protein ORF025875173 & 5174 VWA domain containing CoxE-like protein family ORF02588 5175& 5176 yehQ gene product ORF02589 5177 & 5178 Hypothetical lipoproteinyehR precursor ORF02590 5179 & 5180 uncharacterized protein conserved inbacteria ORF02591 5181 & 5182 unnamed protein product ORF02592 5183 &5184 putative 2-component sensor protein ORF02593 5185 & 5186 MerR-likeregulator A ORF02594 5187 & 5188 glycine betaine-l-proline transportsystem permease protein prow (AE005444) ORF02595 5189 & 5190 putativemembrane protein ORF02596 5191 & 5192 amino acid ABC transporter,ATP-binding protein (AE005444) ORF02597 5193 & 5194 ABC transporter,permease protein (AE005444) ORF02598 5195 & 5196 glycinebetaine-L-proline ABC transporter, periplasmic substrate-bindingprotein, putative ORF02599 5197 & 5198 yohA (STI) [3.2.1.21] ORF026005199 & 5200 D-lactate dehydrogenase ORF02601 5201 & 5202 beta-lactamaseORF02602 5203 & 5204 Protein of unknown function (DUF1282) superfamilyORF02603 5205 & 5206 DedA family protein ORF02604 5207 & 5208 yohF[1.—.—.—] ORF02605 5209 & 5210 Putative channel-filament proteinsORF02606 5211 & 5212 Dihydrouridine synthase (Dus) superfamily ORF026075213 & 5214 LrgA family subfamily, putative ORF02608 5215 & 5216membrane protein, putative ORF02609 5217 & 5218 cytidine deaminase (cdd)[3.5.4.5] ORF02610 5219 & 5220 SanA protein ORF02611 5221 & 5222conserved hypothetical protein ORF02612 5223 & 5224 glutamate synthase,small subunit (NADPH) ORF02613 5225 & 5226 dihydroorotase dehydrogenase(pyrD) ORF02614 5227 & 5228 conserved hypothetical protein ORF02615 5229& 5230 ribose ABC transporter, permease protein (membrane) ORF02616 5231& 5232 Galactoside transport ATP-binding protein mglA (atp_bind)ORF02617 5233 & 5234 D-galactose-binding periplasmic protein precursor(GBP) (D-galactose-D-glucose binding protein) (GGBP) ORF02618 5235 &5236 Mgl repressor and galactose ultrainduction factor ORF02619 5237 &5238 unnamed protein product ORF02620 5239 & 5240 conserved hypotheticalprotein ORF02621 5241 & 5242 GTP cyclohydrolase I (folE) [3.5.4.16]ORF02622 5243 & 5244 carboxylesterase [3.1.1.—] ORF02623 5245 & 5246Colicin I receptor precursor ORF02624 5247 & 5248 lysine-specificpermease ORF02625 5249 & 5250 transcriptional regulator, LysR familyORF02626 5251 & 5252 membrane protein, putative ORF02627 5253 & 5254Endonuclease IV (Endodeoxyribonuclease IV) [3.1.21.2] ORF02628 5255 &5256 yeiI ORF02629 5257 & 5258 inosine-uridine preferring nucleosidehydrolase (AL355920) ORF02630 5259 & 5260 Regulatory protein nsrORF02631 5261 & 5262 nucleoside transporter ORF02632 5263 & 5264uncharacterized enzyme involved in pigment biosynthesis ORF02633 5265 &5266 yeiI ORF02634 5267 & 5268 PTS system, fructose-specific IIBCcomponent [2.7.1.69] ORF02635 5269 & 5270 1-phosphofructokinase(Fructose 1-phosphate kinase) (fruK) [2.7.1.56] ORF02636 5271 & 5272 PTSsystem, fructose-specific IIA-FPr component (EIIA-Fru)(Fructose-permease IIA-FPr component) (Phosphotransferase enzyme II,A-FPrcomponent) (Phosphotransferase FPr protein) (Pseudo-HPr)(EIII-Fru)(Fructose PTS diphosphoryl transfer protein) (EIII-FRU) [2.7.1.69]ORF02637 5273 & 5274 conserved hypothetical protein ORF02638 5275 & 5276Sugar efflux transporter B (orf104) ORF02639 5277 & 5278 translationelongation factor EF-P (EF-P) ORF02640 5279 & 5280 D-MANNONATEOXIDOREDUCTASE ORF02641 5281 & 5282 CobW-P47K family protein domainprotein ORF02642 5283 & 5284 PAP2 superfamily domain protein ORF026435285 & 5286 Lipoprotein spr precursor (spr) ORF02644 5287 & 5288 Rtnprotein ORF02645 5289 & 5290 ABC transporter substrate-binding proteinORF02646 5291 & 5292 peptide ABC transporter, permease protein ORF026475293 & 5294 ABC transporter, permease protein ORF02648 5295 & 5296 ABCtransporter, nucleotide binding-ATPase protein [peptide] ORF02649 5297 &5298 unnamed protein product ORF02650 5299 & 5300 bicyclomycinresistance protein ORF02651 5301 & 5302 ribosomal small subunitpseudouridine synthase A ORF02652 5303 & 5304 yejH ORF02653 5305 & 530650S ribosomal protein L25 ORF02654 5307 & 5308 nucleoid-associatedprotein ORF02655 5309 & 5310 hypothetical protein ORF02656 5311 & 5312Protein of unknown function (DUF1414) superfamily ORF02657 5313 & 5314Predicted hydrolase of alkaline phosphatase superfamily ORF02658 5315 &5316 yejO ORF02659 5317 & 5318 Nitrate-nitrite response regulatorprotein narP ORF02660 5319 & 5320 Cytochrome c-type biogenesis proteinccmH precursor (nrfF) ORF02661 5321 & 5322 Thiol: disulfide interchangeprotein dsbE (Cytochrome c biogenesisprotein ccmG) (dsbE) ORF02662 5323& 5324 Cytochrome c-type biogenesis protein CcmF (ccmF) ORF02663 5325 &5326 Cytochrome c-type biogenesis protein ccmE ORF02664 5327 & 5328 Hemeexporter protein D (Cytochrome c-type biogenesis protein ccmD)-relatedprotein ORF02665 5329 & 5330 heme exporter protein C ORF02666 5331 &5332 heme exporter protein CcmB (ccmB) ORF02667 5333 & 5334 hemeexporter protein CcmA (ccmA) ORF02668 5335 & 5336 Cytochrome c-typeprotein napC ORF02669 5337 & 5338 periplasmic nitrate reductase, dihemeCytochrome c subunit (napB) ORF02670 5339 & 5340 Ferredoxin-type proteinnapH (napH) ORF02671 5341 & 5342 Ferredoxin-type protein napG (napG)ORF02672 5343 & 5344 periplasmic nitrate reductase, large subunit (napA)[1.7.99.4] ORF02673 5345 & 5346 NapD protein (napD) ORF02674 5347 & 5348ferredoxin-type protein NapF (napF) ORF02675 5349 & 5350 Ecotin ORF026765351 & 5352 malate: quinone-oxidoreductase (mqo) [1.1.99.16] ORF026775353 & 5354 hypothetical protein ORF02678 5355 & 5356 MdlB proteinORF02679 5357 & 5358 Alkylated DNA repair protein alkB (alkB) ORF026805359 & 5360 O6-methylguanine-DNA methyltransferase; transcriptionactivator-repressor [2.1.1.63] ORF02681 5361 & 5362 thiaminebiosynthesis lipoprotein ApbE, putative ORF02682 5363 & 5364 Outermembrane protein C precursor (Porin ompC) (Outer membraneprotein 1B)(lbc) ORF02683 5365 & 5366 YojQ [2.7.3.—] ORF02684 5367 & 5368 positiveresponse regulator for colanic capsule biosynthesis ORF02685 5369 & 5370Sensor protein rcsC ORF02686 5371 & 5372 Sensor protein atoS ORF026875373 & 5374 Acetoacetate metabolism Regulatory protein atoC (atoC)ORF02688 5375 & 5376 Acetate CoA-transferase alpha subunit [2.8.3.8]ORF02689 5377 & 5378 Acetate CoA-transferase beta subunit (atoA)[2.8.3.8] ORF02690 5379 & 5380 membrane protein, putative ORF02691 5381& 5382 Acetyl-CoA acetyltransferase (atoB) [2.3.1.9] ORF02692 5383 &5384 conserved hypothetical protein ORF02693 5385 & 5386 conservedhypothetical protein ORF02694 5387 & 5388 Alpha-2-macroglobulin familyN-terminal region family ORF02695 5389 & 5390 Protein of unknownfunction (DUF1175) superfamily ORF02696 5391 & 5392 conservedhypothetical protein ORF02697 5393 & 5394 DNA gyrase, A subunit (gyrA)[5.99.1.3] ORF02698 5395 & 5396 ubiquinone biosynthesisO-methyltransferase (ubiG) [2.1.1.—] ORF02699 5397 & 5398 similar to[SwissProt Accession Number P45508] ORF02700 5399 & 5400 ribonucleosidediphosphate reductase 1, alpha subunit, B1 (nrdA) [1.17.4.1] ORF027015401 & 5402 ribonucleoside diphosphage reductase 1, beta subunit, B2ORF02702 5403 & 5404 adrenodoxin family ferredoxin ORF02703 5405 & 5406Protein inaA ORF02704 5407 & 5408 glycerophosphodiesterphosphodiesterase, periplasmic ORF02705 5409 & 5410 glycerol-3-phosphatetransporter (glpT) ORF02706 5411 & 5412 glpD2, putative [1.1.99.5]ORF02707 5413 & 5414 Anaerobic glycerol-3-phosphate dehydrogenasesubunit B ORF02708 5415 & 5416 Anaerobic glycerol-3-phosphatedehydrogenase subunit C [1.1.99.5] ORF02709 5417 & 5418 conservedhypothetical protein ORF02710 5419 & 5420 transposase ORF02711 5421 &5422 transposase ORF02712 5423 & 5424 similar to [SwissProt AccessionNumber P23522] ORF02713 5425 & 5426 major facilitator family transporterORF02714 5427 & 5428 Mandelate racemase - muconate lactonizing enzyme,C-terminal domain protein ORF02715 5429 & 5430 transcriptionalregulator, IclR family, putative ORF02716 5431 & 5432 CinA-like proteinORF02717 5433 & 5434 YfaZ precursor superfamily ORF02718 5435 & 5436hydrolase, NUDIX family, putative ORF02719 5437 & 5438 protein inducedby aluminum ORF02720 5439 & 5440 lipopolysaccharide biosynthesis proteinORF02721 5441 & 5442 Glycosyl transferase arnC (Ara4N transferase)(Polymixinresistance protein pmrF) [2.—.—.—] ORF02722 5443 & 5444 Formyltransferase, C-terminal domain protein ORF02723 5445 & 5446 PbgP4protein ORF02724 5447 & 5448 melittin resistance protein PqaB ORF027255449 & 5450 sucrose-6 phosphate hydrolase ORF02726 5451 & 5452 conservedhypothetical protein ORF02727 5453 & 5454 Polymyxin B resistance proteinpmrD ORF02728 5455 & 5456 O-succinylbenzoate-CoA ligase (menE)[6.2.1.26] ORF02729 5457 & 5458 o-succinylbenzoic acid (OSB) synthetase(menC) [4.2.1.—] ORF02730 5459 & 5460 naphthoate synthase (menB)[4.1.3.36] ORF02731 5461 & 5462 hydrolase, alpha-beta hydrolase foldfamily, putative ORF02732 5463 & 54642-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acidsynthase-2-oxoglutarate decarboxylase (menD) [4.1.1.71] ORF02733 5465 &5466 Menaquinone-specific isochorismate synthase ORF02734 5467 & 5468ElaB protein ORF02735 5469 & 5470 elaA protein VC2565 (putative)ORF02736 5471 & 5472 metallo-beta-lactamase superfamily protein[3.1.26.11] ORF02737 5473 & 5474 von Willebrand factor type A domainprotein ORF02738 5475 & 5476 putative aminopeptidase ORF02739 5477 &5478 conserved hypothetical protein ORF02740 5479 & 5480 NADHdehydrogenase I chain N (NUO14) [1.6.99.5] ORF02741 5481 & 5482 NADHdehydrogenase I chain M (NUO13) [1.6.99.5] ORF02742 5483 & 5484 NADHdehydrogenase I chain L ECs3162 (NUO12) [1.6.99.5] ORF02743 5485 & 5486NADH dehydrogenase I chain L ECs3162 (NUO12) [1.6.99.5] ORF02744 5487 &5488 NADH dehydrogenase I, K subunit (NUO11) [1.6.5.3] ORF02745 5489 &5490 NADH-quinone oxidoreductase chain J (NADH dehydrogenasel, chain J)(NDH-1, chain J) (NUO10) [1.6.99.5] ORF02746 5491 & 5492 NADHDEHYDROGENASE I CHAIN I (EC 1.6.5.3) (NADH-UBIQUINONE OXIDOREDUCTASECHAIN 9) (NUO9) [1.6.5.3] ORF02747 5493 & 5494 NADH dehydrogenase Ichain H (NUO8) [1.6.99.5] ORF02748 5495 & 5496 NADH DEHYDROGENASE ICHAIN G (EC 1.6.5.3) (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 7) (NUO7)(FRAGMENT) [1.6.5.3] ORF02749 5497 & 5498 NADH-quinone oxidoreductase, Fsubunit (nuoF) ORF02750 5499 & 5500 NADH-quinone oxidoreductase chain E(NADH dehydrogenasel, chain E) (NDH-1, chain E) (NUO5) [1.6.99.5]ORF02751 5501 & 5502 NADH dehydrogenase I chain C-D (NUO4) [1.6.99.5]ORF02752 5503 & 5504 NADH-quinone oxidoreductase chain B (NADHdehydrogenasel, chain B) (NDH-1, chain B) (NUO2) (NUO2) [1.6.99.5]ORF02753 5505 & 5506 NADH-quinone oxidoreductase chain A (NADHdehydrogenasel, chain A) (NDH-1, chain A) (NUO1) [1.6.99.5] ORF027545507 & 5508 LysR family NADH dehydrogenase transcriptional regulator(RssB) ORF02755 5509 & 5510 hypothetical protein ORF02756 5511 & 5512aminotransferase, classes I and II (ASPAT) [2.6.1.—] ORF02757 5513 &5514 b2291 ORF02758 5515 & 5516 unnamed protein product ORF02759 5517 &5518 hydrolase, haloacid dehalogenase-like family [3.1.3.—] ORF027605519 & 5520 Protein yfbU ORF02761 5521 & 5522 Hypothetical UPF0208protein yfbV ORF02762 5523 & 5524 acetate kinase (ackA) [2.7.2.1]ORF02763 5525 & 5526 Phosphate acetyltransferase ORF02764 5527 & 5528predicted membrane protein ORF02765 5529 & 5530 IPP isomerase type 1family protein, putative [3.6.—.—] ORF02766 5531 & 5532 phosphoesterase,putative subfamily ORF02767 5533 & 5534 Glutathione S-transferase,N-terminal domain protein ORF02768 5535 & 5536 glutathioneS-transferase, putative ORF02769 5537 & 5538 dihydroneopterin aldolase(folB) [4.1.2.25] ORF02770 5539 & 5540 conserved hypothetical proteinTIGR01777 ORF02771 5541 & 5542 conserved hypothetical protein subfamilyORF02772 5543 & 5544 ATP-binding component of histidine transportECs3190 ORF02773 5545 & 5546 histidine transport system membrane proteinM (membrane) ORF02774 5547 & 5548 amino acid ABC transporter, permeaseprotein ORF02775 5549 & 5550 Histidine-binding periplasmic proteinprecursor (HBP) ORF02776 5551 & 5552 Lysine-arginine-ornithine-bindingperiplasmic protein precursor (LAO-binding protein) ORF02777 5553 & 55543-octaprenyl-4-hydroxybenzoate carboxy-lyase(Polyprenylp-hydroxybenzoate decarboxylase) [4.1.1.—] ORF02778 5555 &5556 amidophosphoribosyltransferase (purF) [2.4.2.14] ORF02779 5557 &5558 CvpA family protein (cvpA) ORF02780 5559 & 5560 DedD proteinORF02781 5561 & 5562 FolC bifunctional protein [6.3.2.17] ORF02782 5563& 5564 acetyl-CoA carboxylase, carboxyl transferase, beta subunit (accD)[6.4.1.2] ORF02783 5565 & 5566 DedA protein ORF02784 5567 & 5568 tRNApseudouridine synthase A (truA) [4.2.1.70] ORF02785 5569 & 5570 USG-1protein [1.2.1.—] ORF02786 5571 & 5572 Erythronate-4-phosphatedehydrogenase [1.1.1.—] ORF02787 5573 & 5574 hypothetical proteinORF02788 5575 & 5576 Int ORF02789 5577 & 5578 Regulatory protein coxORF02790 5579 & 5580 hypothetical protein ORF02791 5581 & 5582 unknownprotein encoded by prophage CP-933T ORF02792 5583 & 5584 unknown proteinencoded by prophage CP-933T ORF02793 5585 & 5586 unknown protein encodedby prophage CP-933T ORF02794 5587 & 5588 hypothetical protein ORF027955589 & 5590 conserved hypothetical protein ORF02796 5591 & 5592 unknownprotein encoded by prophage CP-933T ORF02797 5593 & 5594 unknown proteinencoded by prophage CP-933T -related protein ORF02798 5595 & 5596conserved hypothetical protein ORF02799 5597 & 5598 Fels-2 prophageprotein ORF02800 5599 & 5600 gpH ORF02801 5601 & 5602 gpU (gpG) ORF028025603 & 5604 gpU prime (gpG) ORF02803 5605 & 5606 DNA-invertase (pin)ORF02804 5607 & 5608 Fels-2 prophage protein (gpU) ORF02805 5609 & 5610phage tail tape meausure protein, TP901 family, putative ORF02806 5611 &5612 probable phage tail protein -related protein ORF02807 5613 & 5614probable tail protein (gpE) ORF02808 5615 & 5616 phage major tail tubeprotein ORF02809 5617 & 5618 Phage tail sheath protein ORF02810 5619 &5620 hypothetical protein ORF02811 5621 & 5622 bacteriophage late genecontrol protein D ORF02812 5623 & 5624 hypothetical protein ORF028135625 & 5626 Phage transcriptional activator, Ogr-Delta ORF02814 5627 &5628 conserved hypothetical protein ORF02815 5629 & 5630 hypotheticalprotein ORF02816 5631 & 5632 hypothetical protein ORF02817 5633 & 5634DIV protein (FRAGMENT) ORF02818 5635 & 5636 Hypothetical UPF0226 proteinyfcJ ORF02819 5637 & 5638 3-oxoacyl-[acyl-carrier-protein] synthase I(Beta-ketoacyl-ACP synthase I) (KAS I) [2.3.1.41] ORF02820 5639 & 5640Glycine-D-amino acid oxidases (deaminating) ORF02821 5641 & 5642conserved hypothetical protein ORF02822 5643 & 5644 similar to[SwissProt Accession Number P44255] ORF02823 5645 & 5646 predictedpermease (ORF9) ORF02824 5647 & 5648 Penicillin-insensitive mureinendopeptidase precursor [3.4.99.—] ORF02825 5649 & 5650 chorismatesynthase (aroC) [4.2.3.5] ORF02826 5651 & 5652 Predicted rRNA or tRNAmethylase [2.1.1.72] ORF02827 5653 & 5654 Smr domain protein ORF028285655 & 5656 conserved hypothetical protein ORF02829 5657 & 5658 minorfimbrial subunit StfG ORF02830 5659 & 5660 minor fimbrial subunit StfFORF02831 5661 & 5662 fimbrial subunit ORF02832 5663 & 5664 periplasmicfimbrial chaperone StfD ORF02833 5665 & 5666 outer membrane usherprotein StfC ORF02834 5667 & 5668 major fimbrial subunit StfA ORF028355669 & 5670 hypothetical protein ORF02836 5671 & 5672 phosphohistidinephosphatase SixA (sixA) [3.1.3.—] ORF02837 5673 & 5674 fatty oxidationcomplex, alpha subunit FadJ (fadJ) [4.2.1.17 1.1.1.35 5.1.2.3] ORF028385675 & 5676 acetyl-CoA C-acyltransferase FadI (fadI) [2.3.1.16] ORF028395677 & 5678 conserved hypothetical protein superfamily ORF02840 5679 &5680 Long-chain fatty acid transport protein precursor (Outer membranefadLprotein) (Outer membrane flp protein) ORF02841 5681 & 5682 VACJLIPOPROTEIN PRECURSOR. (vacJ) ORF02842 5683 & 5684 probable transportyfdC ORF02843 5685 & 5686 putative prophage integrase ORF02844 5687 &5688 hypothetical protein ORF02845 5689 & 5690endo-alpha-sialidase[3.2.1.129] ORF02846 5691 & 5692 hypotheticalprotein ORF02847 5693 & 5694 endo-alpha-sialidase [3.2.1.129] ORF028485695 & 5696 Antirepressor protein ant ORF02849 5697 & 5698 unnamedprotein product; pot. mnt-gene product (aa 1-83) ORF02850 5699 & 5700DNA transfer protein ORF02851 5701 & 5702 hypothetical protein ORF028525703 & 5704 DNA transfer protein ORF02853 5705 & 5706 DNA transferprotein gp7 ORF02854 5707 & 5708 head assembly protein ORF02855 5709 &5710 DNA stabilization protein ORF02856 5711 & 5712 DNA stabilizationprotein ORF02857 5713 & 5714 gene 7 protein ORF02858 5715 & 5716hypothetical protein ORF02859 5717 & 5718 coat protein, putativeORF02860 5719 & 5720 P23, putative ORF02861 5721 & 5722 portal proteinORF02862 5723 & 5724 phage terminase, large subunit, PBSX familyORF02863 5725 & 5726 conserved hypothetical protein ORF02864 5727 & 5728gene 67 protein ORF02865 5729 & 5730 alternate start at bp 59; ORFORF02866 5731 & 5732 Bacteriophage lysis protein ORF02867 5733 & 5734lysozyme [3.2.1.17] ORF02868 5735 & 5736 phage holin, lambda familyORF02869 5737 & 5738 gene 59 protein ORF02870 5739 & 5740 Phage NinHprotein superfamily ORF02871 5741 & 5742 holiday-junction resolvase[3.1.22.—] ORF02872 5743 & 5744 Gp66 ORF02873 5745 & 5746 DNA-bindingprotein Roi (Ant1) ORF02874 5747 & 5748 NinF protein superfamilyORF02875 5749 & 5750 NinX ORF02876 5751 & 5752 Protein ninE-relatedprotein ORF02877 5753 & 5754 Protein ninB ORF02878 5755 & 5756 DnaBanalogue [3.6.1.—] ORF02879 5757 & 5758 Gp54 ORF02880 5759 & 5760Regulatory protein CII (cII) ORF02881 5761 & 5762 hypothetical proteinORF02882 5763 & 5764 gene 33 protein ORF02883 5765 & 5766 gene 33protein ORF02884 5767 & 5768 KiI protein-related protein ORF02885 5769 &5770 Gp42.1 ORF02886 5771 & 5772 Gp40 ORF02887 5773 & 5774 gene 25protein ORF02888 5775 & 5776 hypothetical protein ORF02889 5777 & 5778Gp37 ORF02890 5779 & 5780 Protein of unknown function (DUF551) familyORF02891 5781 & 5782 conserved hypothetical protein ORF02892 5783 & 5784gene 18 protein-related protein ORF02893 5785 & 5786 D-serine deaminasetranscriptional activator (dsdC) ORF02894 5787 & 5788 DsdX permeaseORF02895 5789 & 5790 D-serine ammonia-lyase (dsdA) [4.3.1.18] ORF028965791 & 5792 Multidrug resistance protein Y ORF02897 5793 & 5794Multidrug resistance protein K ORF02898 5795 & 5796 conservedhypothetical protein ORF02899 5797 & 5798 Positive transcriptionregulator evgA ORF02900 5799 & 5800 Sensor protein evgS precursor[2.7.3.—] ORF02901 5801 & 5802 CAIB-BAIF family protein ORF02902 5803 &5804 similar to [SwissProt Accession Number P45869] ORF02903 5805 & 5806oxcA [4.1.1.8] ORF02904 5807 & 5808 CAIB-BAIF family protein [2.8.3.16]ORF02905 5809 & 5810 YfdX protein precursor ORF02906 5811 & 5812conserved hypothetical protein ORF02907 5813 & 5814 DDG protein ORF029085815 & 5816 transaminase [similarity] (ASPAT) [2.6.1.—] ORF02909 5817 &5818 Histidine kinase family ORF02910 5819 & 5820 Autolysin responseregulator ORF02911 5821 & 5822 transcription regulator VC1825 ORF029125823 & 5824 Phosphoenolpyruvate-protein phosphotransferaseptsA(Phosphotransferase system, enzyme I) (Enzyme I-Ani) [2.7.3.9]ORF02913 5825 & 5826 frv operon protein ORF02914 5827 & 5828 prolinedipeptidase [3.4.—.—] ORF02915 5829 & 5830 PTS system, fructose specificIIABC components, putative [2.7.1.69] ORF02916 5831 & 5832 PTS system,fructose-like-2 IIB component 1 (phosphotransferase ORF02917 5833 & 5834glucokinase (glk) [2.7.1.2] ORF02918 5835 & 5836 Putative ion-transportprotein yfeO ORF02919 5837 & 5838 conserved hypothetical proteinORF02920 5839 & 5840 transport protein, NRAMP family ORF02921 5841 &5842 Nucleoside permease nupC ORF02922 5843 & 5844 Nucleoside permeasenupC ORF02923 5845 & 5846 similar to [SwissProt Accession Number P23842]start codon is not identified yet ORF02924 5847 & 5848 similar to[SwissProt Accession Number P27239] start codon is not identified yetORF02925 5849 & 5850 similar to [SwissProt Accession Number P27239]start codon is not identified yet ORF02926 5851 & 5852 glutamyl-tRNAsynthetase (gltX) [6.1.1.17] ORF02927 5853 & 5854 transcriptionalregulator, LysR family, putative ORF02928 5855 & 5856 conservedhypothetical protein ORF02929 5857 & 5858 nucleoside permease NupGORF02930 5859 & 5860 xanthosine phosphorylase (xapA) [2.4.2.1] ORF029315861 & 5862 hypothetical protein ORF02932 5863 & 5864 Protein of unknownfunction (DUF1384) superfamily ORF02933 5865 & 5866 OsmT proteinORF02934 5867 & 5868 similar to [SwissProt Accession Number P39836]start codon is not identified yet ORF02935 5869 & 5870 conservedhypothetical protein ORF02936 5871 & 5872 DNA ligase, NAD-dependent(ligA) [6.5.1.2] ORF02937 5873 & 5874 cell division protein ZipA (zipA)ORF02938 5875 & 5876 CysZ protein ORF02939 5877 & 5878 cysteine synthaseA (cysK) [2.5.1.47] ORF02940 5879 & 5880 Phosphocarrier protein HPr(Histidine-containing protein) (ptsH) [2.7.1.69] ORF02941 5881 & 5882phosphoenolpyruvate-protein phosphotransferase (ptsl) [2.7.3.9] ORF029425883 & 5884 phosphotransferase system enzyme II, glucose-specific,factor III (crr) [2.7.1.69] ORF02943 5885 & 5886 pyridoxal kinase[2.7.1.35] ORF02944 5887 & 5888 conserved hypothetical protein ORF029455889 & 5890 cysteine synthase B (cysM) [2.5.1.47] ORF02946 5891 & 5892Sulfate-thiosulfate import ATP-binding protein cysA(Sulfate-transportingATPase) (atp_bind) [3.6.3.25] ORF02947 5893 & 5894 sulfate ABCtransporter, permease protein CysW (cysW) ORF02948 5895 & 5896 sulfateABC transporter, permease protein CysT (cysT) ORF02949 5897 & 5898thiosulfate binding protein ORF02950 5899 & 59003-oxoacyl-(acyl-carrier-protein) reductase ORF02951 5901 & 5902glucokinase regulator-related protein ORF02952 5903 & 5904 PTS system,sucrose-specific IIBC component (EIIBC-Scr) (Sucrose-permease IIBCcomponent) (Phosphotransferase enzyme II, BC component)(EC 2.7.1.69)(EII-Scr) [2.7.1.69] ORF02953 5905 & 5906 tyrA protein VC2145 ORF029545907 & 5908 Protein of unknown function (DUF1131) superfamily ORF029555909 & 5910 conserved hypothetical protein ORF02956 5911 & 5912acetyltransferase, GNAT family family ORF02957 5913 & 5914N-acetylmuramoyl-l-alanineamidase I [3.5.1.28] ORF02958 5915 & 5916coproporphyrinogen III oxidase, aerobic (hemF) [1.3.3.3] ORF02959 5917 &5918 Ethanolamine operon Regulatory protein ORF02960 5919 & 5920Ethanolamine utilization protein eutK precursor ORF02961 5921 & 5922Ethanolamine utilization protein eutL (eutL) ORF02962 5923 & 5924ethanolamine ammonia-lyase, light subunit, putative [4.3.1.7] ORF029635925 & 5926 ethanolamine ammonia-lyase, large subunit (eutB) [4.3.1.7]ORF02964 5927 & 5928 Ethanolamine utilization protein eutA ORF02965 5929& 5930 Ethanolamine utilization protein eutH ORF02966 5931 & 5932Ethanolamine utilization protein eutG ORF02967 5933 & 5934 Ethanolamineutilization protein eutJ (EutJ) ORF02968 5935 & 5936 aldehyde-alcoholdehydrogenase (EutE) ORF02969 5937 & 5938 carbon dioxide concentratingmechanism protein CcmL, putative ORF02970 5939 & 5940 detox protein(EutM) ORF02971 5941 & 5942 phosphate acetyltransferase (pta) [2.3.1.8]ORF02972 5943 & 5944 Ethanolamine utilization cobalaminadenosyltransferase [2.5.1.17] ORF02973 5945 & 5946 Ethanolamineutilization protein eutQ ORF02974 5947 & 5948 Ethanolamine utilizationprotein eutP ORF02975 5949 & 5950 ethanolamine utilization protein eutSORF02976 5951 & 5952 NADP-dependent malic enzyme (NADP-ME) [1.1.1.40]ORF02977 5953 & 5954 transaldolase (tal) [2.2.1.2] ORF02978 5955 & 5956transketolase (tkt) [2.2.1.1] ORF02979 5957 & 5958 Protein of unknownfunction (DUF1176) superfamily ORF02980 5959 & 5960 conservedhypothetical protein ORF02981 5961 & 5962 conserved hypothetical proteinTIGR00052 ORF02982 5963 & 5964 similar to [SwissProt Accession NumberP37127] start codon is not identified yet (GltD) [1.4.1.131 ORF029835965 & 5966 Nitrate-nitrite sensor protein narQ ORF02984 5967 & 59681037 amino acids, 113 kDa protein ORF02985 5969 & 5970 Protein yffB[1.—.—.—] ORF02986 5971 & 5972 succinyl-diaminopimelate desuccinylase(dapE) [3.5.1.18] ORF02987 5973 & 5974 conserved hypothetical proteinORF02988 5975 & 5976 3.1.—.— [3.1.—.—] ORF02989 5977 & 5978 similar to[SwissProt Accession Number P44140] ORF02990 5979 & 5980metallopeptidase, zinc binding [3.4.—.—] ORF02991 5981 & 5982phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)[6.3.2.6] ORF02992 5983 & 5984 Lipoprotein-34 precursor ORF02993 5985 &5986 dihydrodipicolinate synthase (dapA) [4.2.1.52] ORF02994 5987 & 5988transcriptional regulation of gcv operon ORF02995 5989 & 5990 bcp[1.11.1.—] ORF02996 5991 & 5992 predicted permease ORF02997 5993 & 5994Peptidase family M48 family [3.4.—.—] ORF02998 5995 & 5996 arsenatereductase (arsC) [1.20.4.1] ORF02999 5997 & 5998 ATPase involved in DNAreplication initiation ORF03000 5999 & 6000 Uracil permease ORF030016001 & 6002 uracil phosphoribosyltransferase (upp) [2.4.2.9] ORF030026003 & 6004 phosphoribosylformylglycinamidine cyclo-ligase (purM)[6.3.3.1] ORF03003 6005 & 6006 phosphoribosylglycinamideformyltransferase (purN) [2.1.2.2] ORF03004 6007 & 6008 polyphosphatekinase (ppk) [2.7.4.1] ORF03005 6009 & 6010 exopolyphosphatase[3.6.1.11] ORF03006 6011 & 6012 MASE1 domain family ORF03007 6013 & 6014conserved hypothetical protein ORF03008 6015 & 6016 hypothetical proteinORF03009 6017 & 6018 putative outer membrane lipoprotein ORF03010 6019 &6020 probable membrane protein Z3770 ORF03011 6021 & 6022 putativeRz-like protein ORF03012 6023 & 6024 endolysin ORF03013 6025 & 6026holin ORF03014 6027 & 6028 endo-alpha-sialidase ORF03015 6029 & 6030hypothetical protein ORF03016 6031 & 6032 endo-alpha-sialidase[3.2.1.129] ORF03017 6033 & 6034 hypothetical protein ORF03018 6035 &6036 Gp27 ORF03019 6037 & 6038 hypothetical protein ORF03020 6039 & 6040hypothetical protein ORF03021 6041 & 6042 hypothetical protein ORF030226043 & 6044 conserved hypothetical protein ORF03023 6045 & 6046conserved hypothetical protein ORF03024 6047 & 6048 hypothetical proteinORF03025 6049 & 6050 Bbp13 ORF03026 6051 & 6052 gp11 ORF03027 6053 &6054 Bbp20 ORF03028 6055 & 6056 conserved hypothetical protein ORF030296057 & 6058 Bbp16 ORF03030 6059 & 6060 gp08 ORF03031 6061 & 6062 Bbp18ORF03032 6063 & 6064 hypothetical protein ORF03033 6065 & 6066 Bbp19ORF03034 6067 & 6068 Bbp21 ORF03035 6069 & 6070 conserved hypotheticalprotein ORF03036 6071 & 6072 hypothetical protein ORF03037 6073 & 6074hypothetical protein ORF03038 6075 & 6076 terminase large subunitORF03039 6077 & 6078 conserved hypothetical protein ORF03040 6079 & 6080putative repressor ORF03041 6081 & 6082 conserved hypothetical proteinORF03042 6083 & 6084 conserved hypothetical protein ORF03043 6085 & 6086exonuclease VIII RecE [3.1.11.—] ORF03044 6087 & 6088 enterohemolysin 1ORF03045 6089 & 6090 Prophage CP4-57 regulatory protein (AlpA) familyORF03046 6091 & 6092 conserved hypothetical protein ORF03047 6093 & 6094adenine methylase ORF03048 6095 & 6096 adenine methylase ORF03049 6097 &6098 integrase ORF03050 6099 & 6100 GMP synthase [glutamine-hydrolyzing](Glutamineamidotransferase) (GMP synthetase) (glutamine-hydro) [6.3.5.2]ORF03051 6101 & 6102 inosine-5′-monophosphate dehydrogenase (guaB)[1.1.1.205] ORF03052 6103 & 6104 exodeoxyribonuclease VII, large subunit(xseA) [3.1.11.6] ORF03053 6105 & 6106 Aec4 precursor ORF03054 6107 &6108 SinI homolog ORF03055 6109 & 6110 Aec1 precursor ORF03056 6111 &6112 Protein of unknown function (DUF1407) superfamily ORF03057 6113 &6114 predicted GTP-binding protein ORF03058 6115 & 6116 PQQ enzymerepeat domain protein [2.7.1.—] ORF03059 6117 & 6118 unnamed proteinproduct ORF03060 6119 & 6120 histidyl-tRNA synthetase (hisS) [6.1.1.21]ORF03061 6121 & 6122 4-hydroxy-3-methylbut-2-en-1-yl diphosphatesynthase (ispG) [1.17.4.3] ORF03062 6123 & 6124 similar to [SwissProtAccession Number P27434] ORF03063 6125 & 6126 radical SAM enzyme, Cfrfamily ORF03064 6127 & 6128 Nucleoside diphosphate kinase (ndk)[2.7.4.6] ORF03065 6129 & 6130 penicillin-binding protein 1C (pbpC)ORF03066 6131 & 6132 Hypothetical lipoprotein yfhM precursor ORF030676133 & 6134 3-mercaptopyruvate sulfurtransferase (AF109156) [2.8.1.1]ORF03068 6135 & 6136 enhanced serine sensitivity ORF03069 6137 & 6138Cytosol aminopeptidase family, catalytic domain family ORF03070 6139 &6140 Protein of unknown function (DUF528) superfamily ORF03071 6141 &6142 ferredoxin, 2Fe—2S type, ISC system (fdx) ORF03072 6143 & 6144 Fe—Sprotein assembly chaperone HscA (hscA) ORF03073 6145 & 6146 Fe—S proteinassembly co-chaperone HscB (hscB) ORF03074 6147 & 6148 iron-sulfurcluster assembly protein IscA (iscA) ORF03075 6149 & 6150 FeS clusterassembly scaffold IscU (iscU) ORF03076 6151 & 6152 cysteine desulfuraseIscS (iscS) [4.4.1.—] ORF03077 6153 & 6154 iron-sulfur cluster assemblytranscription factor IscR (iscR) ORF03078 6155 & 6156 RNAmethyltransferase, TrmH family, group 1 ORF03079 6157 & 6158Inositol-1-monophosphatase (IMPase) (Inositol-1-phosphatase) (I-1-Pase)(I-1-Pase) [3.1.3.25] ORF03080 6159 & 6160 conserved hypotheticalprotein ORF03081 6161 & 6162 MFS (major facilitator superfamily)transporter ORF03082 6163 & 6164 csiE ORF03083 6165 & 6166 DoxXsubfamily, putative ORF03084 6167 & 6168 Aldose 1-epimerase superfamilyORF03085 6169 & 6170 oxidoreductase, zinc-binding dehydrogenase familysuperfamily [1.1.1.14] ORF03086 6171 & 6172 ribose ABC transporter,permease protein ORF03087 6173 & 6174 D-xylose ABC transporter,ATP-binding protein ORF03088 6175 & 6176 ribose ABC transporter,periplasmic ribose-binding protein, putative ORF03089 6177 & 6178 TPRDomain domain protein ORF03090 6179 & 6180 ROK family protein domainprotein ORF03091 6181 & 6182 serine hydroxymethyltransferase (glyA)[2.1.2.1] ORF03092 6183 & 6184 dihydropteridine reductase (NOD)[1.5.1.34] ORF03093 6185 & 6186 Nitrogen regulatory protein P-IIORF03094 6187 & 6188 transcriptional regulatory protein ORF03095 6189 &6190 unnamed protein product ORF03096 6191 & 6192 two-component systemsensor kinase ORF03097 6193 & 6194 phosphoribosylformylglycinamidinesynthase (purL) [6.3.5.3] ORF03098 6195 & 6196 transglycosylase, Sltfamily ORF03099 6197 & 6198 tRNA-specific adenosine deaminase [3.5.4.—]ORF03100 6199 & 6200 HAD superfamily (subfamily IF) hydrolase, YfhB(yfhb) ORF03101 6201 & 6202 Transcriptional regulator (membrane)ORF03102 6203 & 6204 NapF protein (fdx) ORF03103 6205 & 6206holo-(acyl-carrier-protein) synthase (acpS) [2.7.8.7] ORF03104 6207 &6208 pyridoxal phosphate biosynthetic protein PdxJ (pdxJ) ORF03105 6209& 6210 DNA repair protein RecO (recO) ORF03106 6211 & 6212 GTP-bindingprotein Era (era) ORF03107 6213 & 6214 ribonuclease III (rnc) [3.1.26.3]ORF03108 6215 & 6216 signal peptidase I [similarity] (lep) [3.4.21.89]ORF03109 6217 & 6218 GTP-binding protein LepA (lepA) ORF03110 6219 &6220 Sigma-E factor Regulatory protein rseC ORF03111 6221 & 6222 Sigma-Efactor Regulatory protein rseB precursor (rseB) ORF03112 6223 & 6224sigma-E factor, negative regulatory protein ORF03113 6225 & 6226 RNApolymerase sigma E (SIGMA) ORF03114 6227 & 6228 hypothetical proteinORF03115 6229 & 6230 unnamed protein product ORF03116 6231 & 6232L-aspartate oxidase (nadB) [1.4.3.16] ORF03117 6233 & 6234 ATP-dependentRNA helicase (srmB) ORF03118 6235 & 6236 similar to [SwissProt AccessionNumber P33634] ORF03119 6237 & 6238 translocator protein, LysE familysuperfamily ORF03120 6239 & 6240 Protein yfiD ORF03121 6241 & 6242uracil-DNA glycosylase (ung) [3.2.2.—] ORF03122 6243 & 6244 similar to[SwissProt Accession Number P33635] start codon is not identified yetORF03123 6245 & 6246 hypothetical protein ORF03124 6247 & 6248thioredoxin (trx) ORF03125 6249 & 6250 DTW domain protein ORF03126 6251& 6252 Acyl-CoA synthetase (NDP forming) ORF03127 6253 & 6254CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE (EC 2.7.8.8)(PHOSPHATIDYLSERINE SYNTHASE). [2.7.8.8] ORF03128 6255 & 6256lipoprotein, putative ORF03129 6257 & 6258 Alpha-ketoglutarate permeaseORF03130 6259 & 6260 transposase ORF03131 6261 & 6262 transposaseORF03132 6263 & 6264 transposase ORF03133 6265 & 6266 hypotheticalORF03134 6267 & 6268 hypothetical protein ORF03135 6269 & 6270 heatshock protein [3.4.21.—] ORF03136 6271 & 6272 conserved hypotheticalprotein TIGR00726 ORF03137 6273 & 6274 ribosomal large subunitpseudouridine synthase D (sfhB) [4.2.1.70] ORF03138 6275 & 6276 unkownORF03139 6277 & 6278 ribosomal subunit interface protein (yfiA) ORF031406279 & 6280 P-protein (PDT) [5.4.99.5] ORF03141 6281 & 6282 T-protein(PDH) [5.4.99.5] ORF03142 6283 & 6284 phospho-2-dehydro-3-deoxyheptonatealdolase [2.5.1.54] ORF03143 6285 & 6286 lipoprotein, putative ORF031446287 & 6288 conserved hypothetical protein ORF03145 6289 & 6290 similarto [SwissProt Accession Number P46139] ORF03146 6291 & 6292 unnamedprotein product; unidentified reading frame (16K polypeptide) (aa 1-127)ORF03147 6293 & 6294 ribosomal protein L19 (rplS) ORF03148 6295 & 6296tRNA (guanine-N1)-methyltransferase (trmD) [2.1.1.31] ORF03149 6297 &6298 16S rRNA processing protein RimM (rimM) ORF03150 6299 & 6300 30Sribosomal protein S16 (rpS16) ORF03151 6301 & 6302 signal recognitionparticle protein (ffh) ORF03152 6303 & 6304 CorE ORF03153 6305 & 6306unnamed protein product ORF03154 6307 & 6308 co-chaperone GrpE (grpE)ORF03155 6309 & 6310 NAD+ kinase [2.7.1.23] ORF03156 6311 & 6312hypothetical protein ORF03157 6313 & 6314 DNA repair protein RecN (recN)ORF03158 6315 & 6316 Small protein A precursor ORF03159 6317 & 6318Protein yfjF ORF03160 6319 & 6320 Streptomyces cyclase-dehydrase familyORF03161 6321 & 6322 SsrA-binding protein (smpB) ORF03162 6323 & 6324hypothetical protein ORF03163 6325 & 6326 conserved hypothetical proteinORF03164 6327 & 6328 DinI-like protein Z3916-ECs3483 (AF175466) ORF031656329 & 6330 T4-exclusion protein stimulator gIBEGs ORF03166 6331 & 6332ycfE protein (fragment) ORF03167 6333 & 6334 unnamed protein product;unidentified reading frame P-293 ORF03168 6335 & 6336 heat shock protein[3.4.21.—] ORF03169 6337 & 6338 heat shock protein [3.4.21.—] ORF031706339 & 6340 conserved hypothetical protein ORF03171 6341 & 6342 Sb36ORF03172 6343 & 6344 conserved hypothetical protein ORF03173 6345 & 6346hypothetical protein ORF03174 6347 & 6348 conserved hypothetical proteinORF03175 6349 & 6350 unnamed protein product ORF03176 6351 & 6352hypothetical protein ORF03177 6353 & 6354 gab protein ORF03178 6355 &6356 predicted dehydrogenase ORF03179 6357 & 6358 Succinate-semialdehydedehydrogenase [NADP+] [1.2.1.16] ORF03180 6359 & 6360 4-aminobutyratetransaminase (gabT) [2.6.1.19] ORF03181 6361 & 6362 GABA permease (gabP)ORF03182 6363 & 6364 transcriptional regulator, GntR family, putativeORF03183 6365 & 6366 Putative phospholipid-binding domain familyORF03184 6367 & 6368 Hypothetical UPF0057 protein yqaE-related proteinORF03185 6369 & 6370 transcriptional regulator, ArsR family (AJ001934)ORF03186 6371 & 6372 rhodanese family protein, putative ORF03187 6373 &6374 DNA-binding protein stpA ORF03188 6375 & 6376 Protein of unknownfunction (DUF1144) superfamily ORF03189 6377 & 6378 similar to[SwissProt Accession Number P36931] start codon is not identified yetORF03190 6379 & 6380 unnamed protein product; ORFA ORF03191 6381 & 6382GntR-family transcriptional regulator (X78503) ORF03192 6383 & 6384Uncharacterized ACR ORF03193 6385 & 6386 nrdH-related protein ORF031946387 & 6388 nrdI protein (nrdI) ORF03195 6389 & 6390ribonucleoside-diphosphate reductase alpha chain ORF03196 6391 & 6392ribonucleoside-diphosphate reductase, beta subunit [1.17.4.1] ORF031976393 & 6394 ATP-binding component of transport system for glycine,betaine and proline (atp_bind) ORF03198 6395 & 6396 Glycinebetaine-L-proline transport system permease protein proW (membrane)ORF03199 6397 & 6398 high-affinity glycine betaine - proline transportsystem ORF03200 6399 & 6400 major facilitator family transporter(permease) ORF03201 6401 & 6402 AzlC family protein, putative ORF032026403 & 6404 YgaH protein ORF03203 6405 & 6406 Transcriptional repressormprA (EmrR protein) ORF03204 6407 & 6408 Multidrug resistance protein AORF03205 6409 & 6410 Multidrug resistance protein B ORF03206 6411 & 6412conserved hypothetical protein ORF03207 6413 & 6414 conservedhypothetical protein ORF03208 6415 & 6416 autoinducer-2 productionprotein LuxS (luxS) ORF03209 6417 & 6418 glutamate--cysteine ligase(gshA) [6.3.2.2] ORF03210 6419 & 6420 gamma-glutamate-cysteine ligase(GCS) ORF03211 6421 & 6422 conserved hypothetical protein ORF03212 6423& 6424 CbbY family protein VCA0662 (pgp) ORF03213 6425 & 6426 carbonstorage regulator (csrA) ORF03214 6427 & 6428 alanyl-tRNAsynthetase(alaS) [6.1.1.7] ORF03215 6429 & 6430 Regulatory protein recX (oraAprotein) (recX) ORF03216 6431 & 6432 recA protein (recA) ORF03217 6433 &6434 Protein ygaD ORF03218 6435 & 6436 Membrane-bound lytic mureintransglycosylase B precursor ORF03219 6437 & 6438 PTS SYSTEM,GLUCITOL-SORBITOL-SPECIFIC IIBC COMPONENT (EIIBC-GUT)(GLUCITOL-SORBITOL-PERMEASE IIBC COMPONENT) (PHOSPHOTRANSFERASE ENZYMEII, BC COMPONENT) (EC 2.7.1.69) (EII- GUT) [2.7.1.691 ORF03220 6439 &6440 PTS SYSTEM, GLUCITOL-SORBITOL-SPECIFIC IIBC COMPONENT (EIIBC-GUT)(GLUCITOL-SORBITOL-PERMEASE IIBC COMPONENT) (PHOSPHOTRANSFERASE ENZYMEII, BC COMPONENT) (EC 2.7.1.69) (EII- GUT) [2.7.1.69] ORF03221 6441 &6442 PTS enzyme III glucitol [2.7.1.69] ORF03222 6443 & 64443-oxoacyl-(acyl-carrier-protein) reductase (sorbitol) [1.1.1.140]ORF03223 6445 & 6446 Glucitol operon activator protein (gutM) ORF032246447 & 6448 Glucitol operon repressor (srl) ORF03225 6449 & 6450 GutQprotein ORF03226 6451 & 6452 ygaA protein (fhlA) ORF03227 6453 & 6454Anaerobic nitric oxide reductase flavorubredoxin (FlRd) (FlavoRb) (fprA)[1.—.—.—] ORF03228 6455 & 6456 Nitric oxide reductase FlRd-NAD(+)reductase(Flavorubredoxin reductase) (FlRd- reductase) (FlavoRbreductase) (FAD) [1.18.1.—] ORF03229 6457 & 6458 [NiFe] hydrogenasematuration protein HypF (hypF) ORF03230 6459 & 6460 Electron transportprotein hydN ORF03231 6461 & 6462 hypothetical protein ORF03232 6463 &6464 ascBF operon repressor ORF03233 6465 & 6466 PTS system enzyme IIABC (asc) [2.7.1.69] ORF03234 6467 & 6468 6-phospho-beta-glucosidase[3.2.1.86] ORF03235 6469 & 6470 unnamed protein product ORF03236 6471 &6472 conserved hypothetical protein ORF03237 6473 & 6474 hydrogenasematuration protease HycI ORF03238 6475 & 6476 Formate hydrogenlyasematuration protein hycH (HycE) [1.—.—.—] ORF03239 6477 & 6478 Formatehydrogenlyase subunit 7 [1.18.99.1] ORF03240 6479 & 6480 Formatehydrogenlyase subunit 6 [1.18.99.1] ORF03241 6481 & 6482 hydrogenase 3large subunit [1.18.99.1] ORF03242 6483 & 6484 Formate hydrogenlyasesubunit 4 (hycD) [1.18.99.1] ORF03243 6485 & 6486 Formate hydrogenlyasesubunit 3 (FHL subunit 3) (Hydrogenase-3component C) [1.18.99.1]ORF03244 6487 & 6488 formate hydrogenlyase subunit-7 component B (FHL)ORF03245 6489 & 6490 Formate hydrogenlyase regulatory protein hycAORF03246 6491 & 6492 hydrogenase nickel insertion protein HypA (hypA)ORF03247 6493 & 6494 hydrogenase accessory protein HypB ORF03248 6495 &6496 hydrogenase assembly chaperone HypC-HupF (hypC) ORF03249 6497 &6498 hydrogenase expression-formation protein HypD (hypD) ORF03250 6499& 6500 hydrogenase expression-formation protein HypE (hypE) ORF032516501 & 6502 Formate hydrogenlyase transcriptional activator ORF032526503 & 6504 Potential molybdenum-pterin-binding-protein ORF03253 6505 &6506 conserved hypothetical protein ORF03254 6507 & 6508 DNA mismatchrepair protein MutS (mutS) ORF03255 6509 & 6510 Serine-threonine proteinphosphatase 2 [3.1.3.16] ORF03256 6511 & 6512 Transcriptional regulatorof sugar metabolism ORF03257 6513 & 6514 3-hydroxyisobutyratedehydrogenase [1.1.—.—] ORF03258 6515 & 6516 tRNA synthase-like proteinORF03259 6517 & 6518 L-fuculose-1-phosphate aldolase-like protein (fucA)[4.1.2.17] ORF03260 6519 & 6520 Hfi protein [5.3.1.22] ORF03261 6521 &6522 gluconate permease ORF03262 6523 & 6524 metallo-beta-lactamasesuperfamily domain protein ORF03263 6525 & 6526 tryptophan repressorbinding protein ORF03264 6527 & 6528 sigma factor RpoS (sigma38)ORF03265 6529 & 6530 sigma factor RpoS (sigma38) ORF03266 6531 & 6532Lipoprotein nlpD precursor ORF03267 6533 & 6534 protein-L-isoaspartateO-methyltransferase (pcm) [2.1.1.77] ORF03268 6535 & 6536 acidphosphatase SurE (surE) [3.1.3.2] ORF03269 6537 & 6538 conservedhypothetical protein TIGR00094 ORF03270 6539 & 65402C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) [4.6.1.12]ORF03271 6541 & 6542 2-C-methyl-D-erythritol 4-phosphatecytidylyltransferase (ispD) [2.7.7.60] ORF03272 6543 & 6544 Celldivision protein ftsB ORF03273 6545 & 6546 YgbE ORF03274 6547 & 6548adenylylsulfate kinase (cysC) [2.7.1.25] ORF03275 6549 & 6550 Sulfateadenylyltransferase subunit 1 (Sulfate adenylatetransferase) (SAT) (ATP-sulfurylase large subunit) (SAT) [2.7.7.4] ORF03276 6551 & 6552 Sulfateadenylyltransferase subunit 2 (Sulfate adenylatetransferase) (SAT) (ATP-sulfurylase small subunit) (SAT) [2.7.7.4] ORF03277 6553 & 6554hypothetical protein ORF03278 6555 & 6556 Alkaline phosphatase isozymeconversion protein precursor [3.4.11.—] ORF03279 6557 & 6558 Hok-geffamily superfamily ORF03280 6559 & 6560 3-phosphoadenosine5-phosphosulfate reductase ORF03281 6561 & 6562 sulfite reductase(NADPH) hemoprotein, beta subunit (cysl) [1.8.1.2] ORF03282 6563 & 6564sulfite reductase [NADPH] flavoprotein, alpha chain [1.8.1.2] ORF032836565 & 6566 6-pyruvoyl-tetrahydropterin synthase ORF03284 6567 & 6568Probable electron transfer flavoprotein-quinone oxidoreductase ygcNORF03285 6569 & 6570 Ferredoxin-like protein ygcO ORF03286 6571 & 6572glycerol uptake operon antiterminator regulatory protein, putativeORF03287 6573 & 6574 electron transfer flavoprotein, alpha subunitsubfamily (etfA) ORF03288 6575 & 6576 putative transport proteinORF03289 6577 & 6578 sugar transporter, putative ORF03290 6579 & 6580alkyl-dihydroxyacetonephosphate synthase, putative ORF03291 6581 & 65821.—.—.— [1.1.1.—] ORF03292 6583 & 6584 Major Facilitator Superfamilysubfamily ORF03293 6585 & 6586 sugar kinase, FGGY family, putative[2.7.1.17] ORF03294 6587 & 6588 radical activating enzyme ORF03295 6589& 6590 radical activating enzyme ORF03296 6591 & 6592 LemA familysuperfamily ORF03297 6593 & 6594 glycine rich protein ORF03298 6595 &6596 conserved hypothetical protein ORF03299 6597 & 6598 Domain ofunknown function (DUF477) family ORF03300 6599 & 6600 glycine richprotein ORF03301 6601 & 6602 enolase (eno) [4.2.1.11] ORF03302 6603 &6604 CTP synthase (pyrG) [6.3.4.2] ORF03303 6605 & 6606 MazG protein(mazG) ORF03304 6607 & 6608 PemK-like protein 1 (MazF protein)(AF027767) ORF03305 6609 & 6610 PemI-like protein 1 (MazE protein)ORF03306 6611 & 6612 GTP pyrophosphokinase (ATP: GTP3′-pyrophosphotransferase)(ppGpp synthetase I) ((p)ppGpp synthetase)(relA) [2.7.6.5] ORF03307 6613 & 6614 23S rRNA(uracil-5-)-methyltransferase RumA (rumA) [2.1.1.—] ORF03308 6615 & 6616Sensor protein barA [2.7.3.—] ORF03309 6617 & 6618 hypothetical proteinORF03310 6619 & 6620 glucarate dehydratase [4.2.1.40] ORF03311 6621 &6622 glucarate dehydratase [4.2.1.—] ORF03312 6623 & 6624 MFStransporter, phthalate permease family ORF03313 6625 & 6626 Exoenzymeregulation regulon (mioC) ORF03314 6627 & 6628 RNA pseudouridylatesynthase family protein [4.2.1.70] ORF03315 6629 & 6630 tRNApseudouridine synthase C (Pseudouridylate synthase)(Uracil hydrolyase)[4.2.1.70] ORF03316 6631 & 6632 interacts with secY ORF03317 6633 & 6634hypothetical protein ORF03318 6635 & 6636 GTP cyclohydrolase I-likeprotein ORF03319 6637 & 6638 Predicted Rossmann fold nucleotide-bindingprotein ORF03320 6639 & 6640 Serine transporter ORF03321 6641 & 6642L-serine ammonia-lyase (sdaA) [4.3.1.17] ORF03322 6643 & 6644exodeoxyribonuclease IX ORF03323 6645 & 6646 Lactaldehyde reductase[1.1.1.77] ORF03324 6647 & 6648 L-fuculose phosphate aldolase (fucA)[4.1.2.17] ORF03325 6649 & 6650 conserved hypothetical protein ORF033266651 & 6652 L-fucose permease (fucP) ORF03327 6653 & 6654 L-fucoseisomerase (fucI) [5.3.1.25] ORF03328 6655 & 6656 L-fuculokinase(L-fuculose kinase) (fucK) [2.7.1.51] ORF03329 6657 & 6658 Fucose operonfucU protein (fucLJ) ORF03330 6659 & 6660 hypothetical protein ORF033316661 & 6662 L-fucose operon activator ORF03332 6663 & 6664 SAM-dependentmethyltransferase-like protein ORF03333 6665 & 6666 Uncharacterizedsmall membrane protein ORF03334 6667 & 6668 transcriptional regulator,LysR family (gcvA) ORF03335 6669 & 6670 Bacterial protein of unknownfunction (DUF903) superfamily ORF03336 6671 & 6672 Cysteine sulfinatedesulfinase ORF03337 6673 & 6674 Fe—S metabolism associated domainsubfamily ORF03338 6675 & 6676 Dinucleotide-utilizing enzymes involvedin molybdopterin and thiamine biosynthesis family 1 ORF03339 6677 & 6678Membrane-bound lytic murein transglycosylase A precursor ORF03340 6679 &6680 Protein of unknown function (DUF770) superfamily ORF03341 6681 &6682 Aec18 ORF03342 6683 & 6684 Bacterial protein of unknown function(DUF876) superfamily ORF03343 6685 & 6686 Possibly conserved membraneprotein ORF03344 6687 & 6688 unnamed protein product ORF03345 6689 &6690 Secreted protein Hep ORF03346 6691 & 6692 ClpB protein ORF033476693 & 6694 Rhs element Vgr protein subfamily, putative ORF03348 6695 &6696 unnamed protein product; Similar to unknown protein ORF03349 6697 &6698 unnamed protein product; Highly similar to unknown protein ofPhotorhabdus. Putative secreted protein, putative ORF03350 6699 & 6700unnamed protein product ORF03351 6701 & 6702 unnamed protein productORF03352 6703 & 6704 ImpA-related N-terminal family ORF03353 6705 & 6706conserved hypothetical protein ORF03354 6707 & 6708 hypothetical proteinORF03355 6709 & 6710 Bacterial protein of unknown function (DUF879)superfamily ORF03356 6711 & 6712 Protein of unknown function (DUF1305)superfamily ORF03357 6713 & 6714 lipoprotein, putative ORF03358 6715 &6716 Protein of unknown function (DUF1316) subfamily ORF03359 6717 &6718 conserved hypothetical protein ORF03360 6719 & 6720 ImpA-relatedN-terminal family ORF03361 6721 & 6722 2-hydroxyacid dehydrogenase(serA) ORF03362 6723 & 6724 Phosphosugar isomerase ORF03363 6725 & 6726Beta-cystathionase ORF03364 6727 & 6728 PTS system, IIBC components,putative [2.7.1.69] ORF03365 6729 & 6730 Antiterminator ORF03366 6731 &6732 N-acetylmuramoyl-L-alanine amidase amiC precursor [3.5.1.28]ORF03367 6733 & 6734 amino-acid N-acetyltransferase (argA) [2.3.1.1]ORF03368 6735 & 6736 exodeoxyribonuclease V, alpha subunit (recD)[3.1.11.5] ORF03369 6737 & 6738 exodeoxyribonuclease V, beta subunit(recB) [3.1.11.5] ORF03370 6739 & 6740 Protease III precursor(Pitrilysin) (Protease pi) (pitrilysin) [3.4.24.55] ORF03371 6741 & 6742exodeoxyribonuclease V, gamma subunit (recC) [3.1.11.5] ORF03372 6743 &6744 Prepilin peptidase dependent protein C precursor ORF03373 6745 &6746 conserved hypothetical protein ORF03374 6747 & 6748 unnamed proteinproduct; URF1 (aa 1-267) ORF03375 6749 & 6750 Prepilin peptidasedependent protein A precursor ORF03376 6751 & 6752 thymidylate synthase(thyA) [2.1.1.45] ORF03377 6753 & 6754 prolipoprotein diacylglyceryltransferase (Igt) [2.4.99.—] ORF03378 6755 & 6756Phosphoenolpyruvate-protein phosphotransferase ptsP (Ntr) [2.7.3.9]ORF03379 6757 & 6758 NTP pyrophosphohydrolases including oxidativedamage repair enzymes ORF03380 6759 & 6760 DNA mismatch repairendonuclease mutH (mutH) ORF03381 6761 & 6762 TerC protein ORF03382 6763& 6764 lipoprotein ORF03383 6765 & 6766 Tas protein ORF03384 6767 & 6768unnamed protein product ORF03385 6769 & 6770 AAS bifunctional proteinORF03386 6771 & 6772 Galactose operon repressor ORF03387 6773 & 6774diaminopimelate decarboxylase (lysA) [4.1.1.20] ORF03388 6775 & 6776aspartate racemase subfamily [5.1.1.13] ORF03389 6777 & 6778Transcriptional activator protein lysR ORF03390 6779 & 6780Arabinose-proton symporter ORF03391 6781 & 67822-deoxy-D-gluconate3-dehydrogenase (kduD) [1.1.1.125] ORF03392 6783 &6784 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase ORF03393 6785& 6786 Probable acetyl-CoA acetyltransferase ORF03394 6787 & 6788 serinetransporter ORF03395 6789 & 6790 possible lipoprotein ORF03396 6791 &6792 aldehyde oxidoreductase, putative [1.1.1.204] ORF03397 6793 & 6794Xanthine dehydrogenase, FAD binding subunit (cutB) [1.1.1.204] ORF033986795 & 6796 small chain CO dehydrogenase (cutC) ORF03399 6797 & 6798signal-transduction and transcriptional-control protein ORF03400 6799 &6800 hypothetical protein ORF03401 6801 & 6802 Aspartate-ornithinecarbamoyltransferase, Asp-Orn binding domain family ORF03402 6803 & 6804threonine dehydratase biosynthetic [4.3.1.15] ORF03403 6805 & 6806acetylornithine deacetylase, putative ORF03404 6807 & 6808dihydropyrimidinase (hydA) [3.5.2.2] ORF03405 6809 & 6810 carbamatekinase (arcC) [2.7.2.2] ORF03406 6811 & 6812 xanthine dehydrogenaseaccessory factor, putative subfamily, putative ORF03407 6813 & 6814conserved hypothetical protein ORF03408 6815 & 6816 hypotehtical ygfJORF03409 6817 & 6818 oxidoreductase, pyridine nucleotide-disulfidefamily ORF03410 6819 & 6820 chlorohydrolase family protein, putativeORF03411 6821 & 6822 FAD binding domain in molybdopterin dehydrogenaseprotein ORF03412 6823 & 6824 aldehyde oxidoreductase, putative [1.—.—.—]ORF03413 6825 & 6826 xanthine-uracil permease family protein ORF034146827 & 6828 Guanine deaminase ORF03415 6829 & 6830 xanthine-uracilpermease family protein ORF03416 6831 & 6832 Electron transport proteinhydN ORF03417 6833 & 6834 anaerobically expressed oxidoreductase (GltD)[1.4.1.13] ORF03418 6835 & 6836 xanthine permease ORF03419 6837 & 6838isopentenyl-diphosphate delta-isomerase, type 1 (idi) [5.3.3.2] ORF034206839 & 6840 lysyl-tRNA synthetase (lysS) [6.1.1.6] ORF03421 6841 & 6842peptide chain release factor 2 (prfB) ORF03422 6843 & 6844single-stranded-DNA-specific exonuclease RecJ (recJ) [3.1.—.—] ORF034236845 & 6846 Thiol: disulfide interchange protein dsbC precursor (DsbC)[5.3.4.1] ORF03424 6847 & 6848 tyrosine recombinase XerD (xerD) ORF034256849 & 6850 flavodoxin ORF03426 6851 & 6852 unnamed protein productORF03427 6853 & 6854 Uncharacterized conserved protein ORF03428 6855 &6856 Predicted aminomethyltransferase ORF03429 6857 & 6858 similar tohemolysin III homolog ORF03430 6859 & 6860 UPF0267 protein ORF03431 6861& 6862 6-phospho-beta-glucosidase ORF03432 6863 & 6864 glycinedehydrogenase (gcvP) [1.4.4.2] ORF03433 6865 & 6866 glycine cleavagesystem H protein (gcvH) ORF03434 6867 & 6868 glycine cleavage system Tprotein (gcvT) [2.1.2.10] ORF03435 6869 & 6870 Protein visC [1.—.—.—]ORF03436 6871 & 6872 2-polyprenyl-6-methoxyphenol 4-hydroxylase (ubiH)[1.14.13.—] ORF03437 6873 & 6874 Xaa-Pro aminopeptidase (APP-II)[3.4.11.9] ORF03438 6875 & 6876 yecA family protein subfamily ORF034396877 & 6878 Family of unknown function (DUF710) superfamily ORF034406879 & 6880 5-formyltetrahydrofolate cyclo-ligase family protein,putative ORF03441 6881 & 6882 D-3-phosphoglycerate dehydrogenase[1.1.1.95] ORF03442 6883 & 6884 ribose 5-phosphate isomerase A (rpiA)[5.3.1.6] ORF03443 6885 & 6886 conserved hypothetical protein ORF034446887 & 6888 Chromosome initiation inhibitor ORF03445 6889 & 6890 unnamedprotein product; sbm orf (MCM) ORF03446 6891 & 6892 putativetranscriptional regulator LYSR-type ORF03447 6893 & 6894 IpqG, putativeORF03448 6895 & 6896 possible membrane transport protein ORF03449 6897 &6898 small-conductance mechanosensitive channel ORF03450 6899 & 6900fructose-bisphosphate aldolase, class II (fbaA) [4.1.2.13] ORF03451 6901& 6902 phosphoglycerate kinase (pgk) [2.7.2.3] ORF03452 6903 & 6904D-erythrose-4-phosphate dehydrogenase (epd) [1.2.1.—] ORF03453 6905 &6906 hypothetical protein ORF03454 6907 & 6908 Domain of unknownfunction (DUF296) family ORF03455 6909 & 6910 conserved hypotheticalprotein ORF03456 6911 & 6912 conserved hypothetical protein ORF034576913 & 6914 ABC transporter, ATP-binding protein ORF03458 6915 & 6916kinase-related protein ORF03459 6917 & 6918 Cobalt transport ATP-bindingprotein cbiO ORF03460 6919 & 6920 unnamed protein product; ORF2 (AA1-133) ORF03461 6921 & 6922 fructose-1,6-bisphosphatase, class IIORF03462 6923 & 6924 Putative oxidoreductase ORF03463 6925 & 6926 PTSsystem, mannitol (Cryptic) [2.7.1.69] ORF03464 6927 & 6928 PTS system,mannitol (Cryptic)-specific IIA component (EIIA-(C)Mtl)(Mannitol(Cryptic)-permease IIA component) (Phosphotransferase enzymeII, Acomponent) (Cryptic) [2.7.1.69] ORF03465 6929 & 6930 putative serineprotease ORF03466 6931 & 6932 transketolase (tkt) [2.2.1.1] ORF034676933 & 6934 Putative metalloprotease yggG ORF03468 6935 & 6936agmatinase, putative [3.5.3.11] ORF03469 6937 & 6938 lipoprotein,putative ORF03470 6939 & 6940 arginine decarboxylase (speA) [4.1.1.19]ORF03471 6941 & 6942 conserved hypothetical protein ORF03472 6943 & 6944conserved hypothetical protein ORF03473 6945 & 6946 S-adenosylmethioninesynthetase (metK) [2.5.1.6] ORF03474 6947 & 6948 Galactose-protonsymporter ORF03475 6949 & 6950 Protein sprT ORF03476 6951 & 6952Endonuclease I precursor ORF03477 6953 & 6954 conserved hypotheticalprotein TIGR00046 ORF03478 6955 & 6956 glutathione synthase (gshB)[6.3.2.3] ORF03479 6957 & 6958 Uncharacterized ACR, COG1678 ORF034806959 & 6960 conserved hypothetical protein TIGR00250 ORF03481 6961 &6962 Predicted ATPases involved in pili biogenesis, PilT homologsORF03482 6963 & 6964 conserved hypothetical protein TIGR00044 ORF034836965 & 6966 Predicted integral membrane protein ORF03484 6967 & 6968conserved hypothetical protein TIGR00251 ORF03485 6969 & 6970non-canonical purine NTP pyrophosphatase, rdgB-HAM1 family (rdgB)ORF03486 6971 & 6972 oxygen-independent coproporphyrinogen III oxidase,putative ORF03487 6973 & 6974 Protein of unknown function (DUF1202)superfamily ORF03488 6975 & 6976 L-asparaginase II (ansB) [3.5.1.1]ORF03489 6977 & 6978 conserved hypothetical protein ORF03490 6979 & 6980conserved hypothetical protein ORF03491 6981 & 6982 Protein ORF034926983 & 6984tRNA(guanine-N(7)-)-methyltransferase(tRNA(m7G46)-methyltransferase)[2.1.1.33] ORF03493 6985 & 6986 A-G-specific adenine glycosylaseORF03494 6987 & 6988 Protein yggX ORF03495 6989 & 6990 yggZ protein(mltC) [3.2.1.—] ORF03496 6991 & 6992 nucleoside permease NupG ORF034976993 & 6994 ornithine decarboxylase isozyme (speC) [4.1.1.17] ORF034986995 & 6996 integral membrane protein ORF03499 6997 & 6998 transposase,truncated ORF03500 6999 & 7000 KpsF ORF03501 7001 & 7002 Capsulepolysaccharide export inner-membrane protein kpsE ORF03502 7003 & 7004polysaccharide biosynthesis protein, putative ORF03503 7005 & 70063-deoxy-D-manno-octulosonate cytidylyltransferase (kdsB) [2.7.7.38]ORF03504 7007 & 7008 capsule polysaccharide export protein KpsC ORF035057009 & 7010 KpsS protein ORF03506 7011 & 7012 polysialic acid capsulebiosynthesis protein SiaD, truncation; disrupted by foreign ermC,putative [2.4.—.—] ORF03507 7013 & 7014 NeuE protein ORF03508 7015 &7016 UDP-N-acetylglucosamine 2-epimerase [5.1.3.14] ORF03509 7017 & 7018acylneuraminate cytidylyltransferase, putative [2.7.7.43] ORF03510 7019& 7020 neuB protein [4.1.3.—] ORF03511 7021 & 7022 Polysialic acidtransport ATP-binding protein kpsT [3.6.3.38] ORF03512 7023 & 7024Polysialic acid transport protein kpsM ORF03513 7025 & 7026 Putativegeneral secretion pathway protein M-type yghD ORF03514 7027 & 7028general secretion pathway protein L (gspL) ORF03515 7029 & 7030 Generalsecretion pathway protein K (gspK) ORF03516 7031 & 7032 generalsecretion pathway protein J (gspJ) ORF03517 7033 & 7034 generalsecretion pathway protein I (gspI) ORF03518 7035 & 7036 GspH,hypothetical type II secretion protein ORF03519 7037 & 7038 generalsecretion pathway protein G (gspG) ORF03520 7039 & 7040 generalsecretion pathway protein F (gspF) ORF03521 7041 & 7042 generalsecretion pathway protein E (GSP) ORF03522 7043 & 7044 type II secretorypathway, component EpsD ORF03523 7045 & 7046 general secretion pathwayprotein C (gspC) ORF03524 7047 & 7048 YghG protein ORF03525 7049 & 7050secretion protein b2972 [3.4.23.43] ORF03526 7051 & 7052 Accessorycolonization factor AcfD precursor ORF03527 7053 & 7054 Glycolatepermease glcA ORF03528 7055 & 7056 malate synthase G (glcB) [2.3.3.9]ORF03529 7057 & 7058 glcG protein ORF03530 7059 & 7060 glycolate oxidasesubunits GlcE and GlcF (Fe—S) [1.1.3.15] ORF03531 7061 & 7062 glycolateoxidase subunits GlcE and GlcF ORF03532 7063 & 7064 glycolate oxidase,subunit GlcD (glcD) ORF03533 7065 & 7066 Glc operon transcriptionalactivator ORF03534 7067 & 7068 conserved hypothetical protein ORF035357069 & 7070 acyl-CoA synthase ORF03536 7071 & 7072 conservedhypothetical protein ORF03537 7073 & 7074 Phosphopantetheine attachmentsite (ACP) ORF03538 7075 & 7076 8-amino-7-oxononanoate synthase ORF035397077 & 7078 Predicted permease YjgP-YjgQ family superfamily ORF035407079 & 7080 Predicted permease YjgP-YjgQ family superfamily ORF035417081 & 7082 conserved hypothetical protein YtfJ-family, TIGR01626ORF03542 7083 & 7084 conserved hypothetical protein ORF03543 7085 & 7086Polysaccharide biosynthesis protein domain protein ORF03544 7087 & 7088Hypothetical ATP-binding protein yghR ORF03545 7089 & 7090 HypotheticalATP-binding protein yghS ORF03546 7091 & 7092 hypothetical proteinORF03547 7093 & 7094 Hypothetical ATP-binding protein yghT ORF03548 7095& 7096 low-affinity phosphate transport protein ORF03549 7097 & 7098Glutathionylspermidine synthase ORF03550 7099 & 7100 HypotheticalGST-like protein yghU ORF03551 7101 & 7102 hydrogenase assemblychaperone HypC-HupF (hypC) ORF03552 7103 & 7104 hydrogenase nickelinsertion protein HypA (hypA) ORF03553 7105 & 7106 Hydrogenase-2 operonprotein hybE ORF03554 7107 & 7108 hydrogenase expression-formationprotein ORF03555 7109 & 7110 hydrogenase-2 large subunit HybC[1.18.99.1] ORF03556 7111 & 7112 cytochrome Ni—Fe component ofhydrogenase-2 ORF03557 7113 & 7114 Hydrogenase-2 operon protein hybAprecursor ORF03558 7115 & 7116 Hydrogenase-2 small chain precursor (NiFehydrogenase)(Membrane-bound hydrogenase 2 small subunit) (HYD2) (hydA)[1.12.99.6] ORF03559 7117 & 7118 conserved hypothetical protein ORF035607119 & 7120 dienelactone hydrolase family protein ORF03561 7121 & 7122oxidoreductase, aldo-keto reductase family ORF03562 7123 & 7124conserved hypothetical protein TIGR00645 ORF03563 7125 & 7126oxidoreductase, short chain dehydrogenase-reductase family [1.—.—.—]ORF03564 7127 & 7128 Biopolymer transport exbD protein ORF03565 7129 &7130 biopolymer transport ExbB protein (uptake) ORF03566 7131 & 7132cystathionine beta-lyase (metC) [4.4.1.8] ORF03567 7133 & 7134 unnamedprotein product; Similar to DedA-family integral membrane protein YghBof Escherichia coli ORF03568 7135 & 7136 transcriptional regulator, AraCfamily ORF03569 7137 & 7138 uncharacterized oxidoreductase ORF03570 7139& 7140 oxidoreductase, aldo-keto reductase family [1.1.1.274] ORF035717141 & 7142 conserved hypothetical protein ORF03572 7143 & 7144 majorouter membrane lipoprotein ORF03573 7145 & 7146 radical SAM domainprotein protein ORF03574 7147 & 7148 regulator for uxu operon ORF035757149 & 7150 Hypothetical oxidoreductase ydfl ORF03576 7151 & 71522,3-butanediol dehydrogenase, putative [1.1.1.14] ORF03577 7153 & 7154Ureidoglycolate dehydrogenase [1.1.1.—] ORF03578 7155 & 7156 TRAP-typeC4-dicarboxylate transport system, periplasmic component (AP001509)ORF03579 7157 & 7158 unnamed protein product ORF03580 7159 & 7160 TRAPdicarboxylate transporter, DctM subunit ORF03581 7161 & 7162 suppressorof ftsl ORF03582 7163 & 7164 1-acyl-sn-glycerol-3-phosphateacyltransferase (LPAAT) [2.3.1.51] ORF03583 7165 & 7166 DNAtopoisomerase IV, A subunit (parC) [5.99.1.—] ORF03584 7167 & 7168 ABCsuperfamily (periplasm), oligopeptide transport protein with ORF035857169 & 7170 transcriptional regulator, AraC family ORF03586 7171 & 7172conserved hypothetical protein TIGR00156 ORF03587 7173 & 7174Transcriptional Regulatory protein qseB ORF03588 7175 & 7176 Sensorprotein qseC [2.7.3.—] ORF03589 7177 & 7178 unnamed protein product;Similar to Hcp protein ORF03590 7179 & 7180 NAD(P)H quinoneoxidoreductase, putative ORF03591 7181 & 7182 Protein ygiN ORF03592 7183& 7184 SIS domain protein ORF03593 7185 & 7186 Putative ironcompound-binding protein of ABC transporter ORF03594 7187 & 7188 ironcompound ABC transporter, permease protein (III) ORF03595 7189 & 7190ferric exochelin uptake (fxuC) ORF03596 7191 & 7192 ferric exochelinuptake (fxuB) ORF03597 7193 & 7194 Putative iron compound receptorORF03598 7195 & 7196 DNA topoisomerase IV, B subunit (parE) [5.99.1.—]ORF03599 7197 & 7198 Predicted esterase ORF03600 7199 & 7200 Icc proteinORF03601 7201 & 7202 Protein of unknown function (DUF1249) superfamilyORF03602 7203 & 7204 conserved hypothetical protein TIGR00052 ORF036037205 & 7206 outer membrane channel specific tolerance to colicinORF03604 7207 & 7208 conserved hypothetical protein ORF03605 7209 & 7210glutathionylspermidine synthase (O386) ORF03606 7211 & 7212 arylsulfatesulfotransferase ORF03607 7213 & 7214 Thiol: disulfide interchangeprotein dsbA-like precursor [5.3.4.1] ORF03608 7215 & 7216 disulfidebond formation protein B (dsbB) [1.8.4.—] ORF03609 7217 & 7218 CatalyticLigB subunit of aromatic ring-opening dioxygenase superfamily ORF036107219 & 7220 hypothetical protein ORF03611 7221 & 7222 Zinc transporterzupT ORF03612 7223 & 7224 conserved hypothetical protein ORF03613 7225 &7226 fimbrial protein ORF03614 7227 & 7228 outer membrane fimbrial usherporin ORF03615 7229 & 7230 OUTER MEMBRANE USHER PROTEIN PMFC PRECURSOR.ORF03616 7231 & 7232 Hypothetical fimbrial chaperone yqiH precursorORF03617 7233 & 7234 ybgO protein ORF03618 7235 & 72363,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) ORF03619 7237 &7238 Protein of unknown function (DUF526) family ORF03620 7239 & 7240glycogen biosynthesis, rpoS dependent-related protein ORF03621 7241 &7242 YgiJ ORF03622 7243 & 7244 Uncharacterized BCR, putative ORF036237245 & 7246 ADP-heptose synthase [2.7.—.—] ORF03624 7247 & 7248Glutamate-ammonia-ligase adenylyltransferase ORF03625 7249 & 7250Adenylate cyclase, putative ORF03626 7251 & 7252 conserved hypotheticalprotein ORF03627 7253 & 7254 tRNA nucleotidyl transferase ORF03628 7255& 7256 undecaprenol kinase, putative [2.7.1.66] ORF03629 7257 & 7258dihydroneopterin aldolase (folB) [4.1.2.25] ORF03630 7259 & 7260conserved hypothetical protein TIGR00023 ORF03631 7261 & 7262 LysRfamily regulatory protein ORF03632 7263 & 7264 hypothetical proteinORF03633 7265 & 7266 fumarase (fumarase) [4.2.1.32] ORF03634 7267 & 7268fumarate hydratase, class I, putative [4.2.1.32] ORF03635 7269 & 7270DASS family, citrate: succinate transport (antiport) ORF03636 7271 &7272 O-sialoglycoprotein endopeptidase (gcp) [3.4.24.57] ORF03637 7273 &7274 30S ribosomal protein S21 -related protein ORF03638 7275 & 7276 DNAprimase [2.7.7.—] ORF03639 7277 & 7278 sigma D factor of RNA polymeraseORF03640 7279 & 7280 sigma D factor of RNA polymerase ORF03641 7281 &7282 G-U mismatch-specific DNA glycosylase (Mismatch-specificuracilDNA-glycosylase) (UDG) [3.2.2.—] ORF03642 7283 & 7284 iron-chelatorutilization protein ORF03643 7285 & 7286 transcriptional regulator, PadRfamily ORF03644 7287 & 7288 Aerotaxis receptor ORF03645 7289 & 7290ornithine aminotransferase (rocD) [2.6.1.13] ORF03646 7291 & 7292tRNA-binding protein ygjH (mets) [6.1.1.10] ORF03647 7293 & 7294galactose operon repressor galR ORF03648 7295 & 7296 Evolvedbeta-galactosidase alpha-subunit ORF03649 7297 & 7298 EGB enzyme betasubunit [3.2.1.23] ORF03650 7299 & 7300 probable oxidoreductase ygjIORF03651 7301 & 7302 conserved hypothetical protein ORF03652 7303 & 7304conserved hypothetical protein ORF03653 7305 & 7306 2,4-dienoyl-coAreductase [1.—.—.—] ORF03654 7307 & 7308 2.1.1.52 [2.1.1.52] ORF036557309 & 7310 conserved hypothetical protein ORF03656 7311 & 7312 sanAprotein ORF03657 7313 & 7314 oxidoreductase [1.—.—.—] ORF03658 7315 &7316 Alx protein ORF03659 7317 & 7318 Sodium: dicarboxylate symporterfamily superfamily ORF03660 7319 & 7320 conserved hypothetical proteinORF03661 7321 & 7322 Altronate hydrolase [4.2.1.7] ORF03662 7323 & 7324Glucuronate isomerase (uxaC) [5.3.1.12] ORF03663 7325 & 7326 majorfacilitator family transporter, putative ORF03664 7327 & 7328 Exuregulon transcriptional regulator ORF03665 7329 & 7330 unnamed proteinproduct; Similar to DedA-family integral membrane protein YghB ofEscherichia coli ORF03666 7331 & 7332 conserved hypothetical proteinORF03667 7333 & 7334 Protein yqjC precursor [3.4.24.13] ORF03668 7335 &7336 Bacterial protein of unknown function (DUF883) superfamily ORF036697337 & 7338 conserved hypothetical protein ORF03670 7339 & 7340conserved hypothetical protein ORF03671 7341 & 7342 predicted membraneprotein ORF03672 7343 & 7344 Glutathione S-transferase C terminus (O328)ORF03673 7345 & 7346 Protein of unknown function (DUF805) superfamilyORF03674 7347 & 7348 lysR-family transcription regulatory proteinYPO3545 ORF03675 7349 & 7350 Uncharacterized BCR, YhhW family COG1741family ORF03676 7351 & 7352 conserved hypothetical protein ORF03677 7353& 7354 Protein of unknown function (DUF1063) superfamily ORF03678 7355 &7356 putative transport system permease protein ORF03679 7357 & 7358L-serine ammonia-lyase (sdaA) [4.3.1.17] ORF03680 7359 & 7360endoribonuclease L-PSP, putative ORF03681 7361 & 7362 formateacetyltransferase (pflB) [2.3.1.54] ORF03682 7363 & 7364 acetate kinase(ackA) [2.7.2.1] ORF03683 7365 & 7366 Threonine-serine transporterORF03684 7367 & 7368 Threonine dehydratase catabolic (Threoninedeaminase) (tdcB) [4.3.1.19] ORF03685 7369 & 7370 transcriptionalregulator, LysR family, putative [4.2.1.16] ORF03686 7371 & 7372Glycerate kinase 2 ORF03687 7373 & 7374 2-hydroxy-3-oxopropionatereductase [1.1.1.60] ORF03688 7375 & 7376 2-dehydro-3-deoxyglucaratealdolase (ptpA) [4.1.2.20] ORF03689 7377 & 7378 MFS transporter,phthalate permease family ORF03690 7379 & 7380 D-galactarate dehydratase(GalcD) [4.2.1.42] ORF03691 7381 & 7382 DeoR-family regulatory protein(srl) ORF03692 7383 & 7384 PUTATIVE TAGATOSE 6-PHOSPHATE KINASE PROTEINORF03693 7385 & 7386 N-acetylgalactosamine-specific PTS system enzymeIIB component (EIIB-AGA) [2.7.1.69] ORF03694 7387 & 7388N-acetylgalactosameine-specific IIC component 2 ORF03695 7389 & 7390 PTSsystem, N-acetylgalactosamine-specific IID component ORF03696 7391 &7392 AgaF [2.7.1.69] ORF03697 7393 & 7394N-acetylglucosamine-6-phosphate deacetylase (nagA) [3.5.1.25] ORF036987395 & 7396 sugar isomerase domain protein AgaS [5.—.—.—] ORF03699 7397& 7398 class II aldolase, tagatose bisphosphate family [4.1.2.—]ORF03700 7399 & 7400 PTS system, N-acetylgalactosamine-specific IIBcomponent 1 (EIIB-Aga)(N- acetylgalactosamine-permease IIB component 1)(Phosphotransferaseenzyme II, B component 1) (EIIB-AGA) [2.7.1.69]ORF03701 7401 & 7402 PTS system, N-acetylgalactosamine-specific IICcomponent 1 (EIIC-Aga)(N- acetylgalactosamine-permease IIC component 1)ORF03702 7403 & 7404 PTS system, N-acetylgalactosamine-specific IIDcomponent (EIID-AGA) ORF03703 7405 & 7406 glucosamine-6-phosphateisomerase, putative [5.3.1.—] ORF03704 7407 & 7408 conservedhypothetical protein TIGR00096 ORF03705 7409 & 7410 LppC ORF03706 7411 &7412 conserved hypothetical protein TIGR00252 ORF03707 7413 & 7414phosphoheptose isomerase (gmhA) ORF03708 7415 & 7416 Putativephospholipid-binding domain family ORF03709 7417 & 7418 Predictedpermease superfamily ORF03710 7419 & 7420 conserved hypothetical proteinORF03711 7421 & 7422 unnamed protein product ORF03712 7423 & 7424protease I ORF03713 7425 & 7426 ACETYLTRANSFERASE [2.3.1.—] ORF037147427 & 7428 predicted endonuclease ORF03715 7429 & 7430 SCP-2 steroltransfer family family ORF03716 7431 & 7432 collagenase ORF03717 7433 &7434 peptidase, U32 family family [3.4.—.—] ORF03718 7435 & 7436 flavindependant oxidoreductase ORF03719 7437 & 7438 Tryptophan-specifictransport protein ORF03720 7439 & 7440 ATP-dependent RNA helicase DeaDORF03721 7441 & 7442 Lipoprotein nlpI precursor ORF03722 7443 & 7444Polyribonucleotide nucleotidyltransferase (CAP87K) ORF03723 7445 & 7446ribosomal protein S15 (rpsO) ORF03724 7447 & 7448 tRNA pseudouridinesynthase B [4.2.1.70] ORF03725 7449 & 7450 ribosome-binding factor A(rbfA) ORF03726 7451 & 7452 Translation initiation factor IF-2 ORF037277453 & 7454 Transcription elongation protein nusA (N utilizationsubstance proteinA) (L factor) (nusA) ORF03728 7455 & 7456 YhbC-likeprotein ORF03729 7457 & 7458 argininosuccinate synthase (argG) [6.3.4.5]ORF03730 7459 & 7460 Outer-membrane protein yhbX precursor ORF03731 7461& 7462 Protein-export membrane protein secG ORF03732 7463 & 7464phosphoglucosamine mutase (glmM) [5.4.2.—] ORF03733 7465 & 7466dihydropteroate synthase (folP) [2.5.1.15] ORF03734 7467 & 7468ATP-binding protein [3.4.24.—] ORF03735 7469 & 7470 ribosomal RNA largesubunit methyltransferase J (rrmJ) [2.1.1.—] ORF03736 7471 & 7472conserved hypothetical protein TIGR00253 ORF03737 7473 & 7474Transcription elongation factor greA (Transcript cleavage factorgreA)(greA) ORF03738 7475 & 7476D-alanyl-D-alaninecarboxypeptidase-D-alanyl-D-alanine-endopeptidase(dacB) [3.4.16.4] ORF03739 7477 & 7478 Obg protein (Obg) ORF03740 7479 &7480 Obg protein (F390) ORF03741 7481 & 7482 RhaT protein ORF03742 7483& 7484 RhaT protein ORF03743 7485 & 7486 ribosomal protein L27 (rpmA)ORF03744 7487 & 7488 50S ribosomal protein L21 ORF03745 7489 & 7490conserved hypothetical protein ORF03746 7491 & 7492 RhaT proteinORF03747 7493 & 7494 ribosomal protein L27 (rpmA) ORF03748 7495 & 749650S ribosomal protein L21 ORF03749 7497 & 7498 ribosomal protein L21(rplU) ORF03750 7499 & 7500 conserved hypothetical protein ORF03751 7501& 7502 Octaprenyl-diphosphate synthase (Octaprenylpyrophosphatesynthetase) (OPP synthetase)[2.5.1.—l ORF03752 7503 & 7504Sugar fermentation stimulation protein B (Ner-like protein) ORF037537505 & 7506 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA)[2.5.1.7] ORF03754 7507 & 7508 BolA-like protein ORF03755 7509 & 7510conserved hypothetical protein ORF03756 7511 & 7512 Protein yrbCprecursor (Ttg2D) ORF03757 7513 & 7514 conserved hypothetical proteinORF03758 7515 & 7516 VpsC ORF03759 7517 & 7518 unnamed protein product;Highly similar to ABC transporter, permease protein YrbE of Escherichiacoli ORF03760 7519 & 7520 ABC transporter, ATP-binding protein(atp_bind) ORF03761 7521 & 7522 K+-dependent Na+—Ca+ exchangerrelated-protein ORF03762 7523 & 7524 hypothetical protein ORF03763 7525& 7526 conserved hypothetical protein ORF03764 7527 & 7528 putativeisomerase ORF03765 7529 & 7530 3-deoxy-D-manno-octulosonate 8-phosphatephosphatase(KDO 8-P phosphatase) [3.1.3.45] ORF03766 7531 & 7532 unnamedprotein product; Similar to YrbK precursor protein of Escherichia coliORF03767 7533 & 7534 OstA-like protein family ORF03768 7535 & 7536 ABCtransporter, ATP-binding component ORF03769 7537 & 7538 RNA polymerasesigma-54 factor (rpoN) ORF03770 7539 & 7540 ribosomal subunit interfaceprotein (yfiA) ORF03771 7541 & 7542 PTS IIA-like nitrogen-regulatoryprotein PtsN (ptsN) ORF03772 7543 & 7544 unnamed protein product; ORF193peptide fragment (AA 1-192) (1524 is 2nd base in codon) ORF03773 7545 &7546 phosphocarrier protein (NPr) ORF03774 7547 & 7548 conservedhypothetical protein ORF03775 7549 & 7550 monofunctional biosyntheticpeptidoglycan transglycosylase (mtgA) [2.4.2.—] ORF03776 7551 & 7552sigma cross-reacting protein 27A (SCRP-27A) ORF03777 7553 & 7554 Aerobicrespiration control sensor protein arcB [2.7.3.—] ORF03778 7555 & 7556radical SAM protein, TIGR01212 family ORF03779 7557 & 7558 hypotheticalprotein ORF03780 7559 & 7560 Glutamate synthase [NADPH] large chainprecursor [1.4.1.13] ORF03781 7561 & 7562 glutamate synthase (NADPH)small chain (NADPH) [1.4.1.13] ORF03782 7563 & 7564 conservedhypothetical protein subfamily ORF03783 7565 & 7566 ROK family proteindomain protein, putative ORF03784 7567 & 7568N-acetylmannosamine-6-phosphate 2-epimerase-N-acetylmannosamine kinaseORF03785 7569 & 7570 cis,cis-muconate transport protein MucK, putativeORF03786 7571 & 7572 N-acetylneuraminate lyase (nanA) [4.1.3.3] ORF037877573 & 7574 transcriptional regulator, GntR family, putative ORF037887575 & 7576 stringent starvation protein B (sspB) ORF03789 7577 & 7578Stringent starvation protein A (Ssp) ORF03790 7579 & 7580 ribosomalprotein S9 (rpsI) ORF03791 7581 & 7582 ribosomal protein L13 (rplM)ORF03792 7583 & 7584 Predicted ATPase ORF03793 7585 & 7586 Protein ofunknown function (DUF1043) superfamily ORF03794 7587 & 7588 ProteasedegQ precursor ORF03795 7589 & 7590 periplasmic serine protease DegS(degS) [3.4.21.—] ORF03796 7591 & 7592 malate dehydrogenase,NAD-dependent (mdh) [1.1.1.37] ORF03797 7593 & 7594 arginine repressor(argR) ORF03798 7595 & 7596 Protein of unknown function (DUF1471)superfamily ORF03799 7597 & 7598 unnamed protein product; Similar toribonuclease inhibitor (barstar) ORF03800 7599 & 7600 Fusaric acidresistance protein conserved region family ORF03801 7601 & 7602 fusaricacid resistance protein ORF03802 7603 & 7604 conserved hypotheticalprotein ORF03803 7605 & 7606 conserved hypothetical protein ORF038047607 & 7608 Transcriptional regulator ORF03805 7609 & 7610 TldD protein(CSRA) ORF03806 7611 & 7612 conserved hypothetical protein TIGR02099ORF03807 7613 & 7614 bundles of cytoplasmic filaments ORF03808 7615 &7616 maf protein (maf) ORF03809 7617 & 7618 rod shape-determiningprotein MreD (mreD) ORF03861 7721 & 7722 ribosomal protein S11 (rpsK)ORF03862 7723 & 7724 ribosomal protein S13p-S18e (rpsM) ORF03863 7725 &7726 ribosomal protein L36 (rpmJ) ORF03864 7727 & 7728 preproteintranslocase, SecY subunit ORF03865 7729 & 7730 Preprotein translocasesecY subunit (secY) ORF03866 7731 & 7732 ribosomal protein L15 (rplO)ORF03867 7733 & 7734 ribosomal protein L30 (rpmD) ORF03868 7735 & 7736ribosomal protein S5 (rpsE) ORF03869 7737 & 7738 ribosomal protein L18(rplR) ORF03870 7739 & 7740 50S ribosomal subunit protein L6 (rp1F)ORF03871 7741 & 7742 conserved hypothetical protein ORF03872 7743 & 7744ribosomal protein S8 (rpsH) ORF03873 7745 & 7746 ribosomal proteinS14p-S29e (rpsN) ORF03874 7747 & 7748 50S ribosomal subunit protein L5(rpL5) ORF03875 7749 & 7750 ribosomal protein L24 (rplX) ORF03876 7751 &7752 ribosomal protein L14 (rplN) ORF03877 7753 & 7754 ribosomal proteinS17 (rpsQ) ORF03878 7755 & 7756 ribosomal protein L29 (rpmC) ORF038797757 & 7758 ribosomal protein L16 (rplP) ORF03880 7759 & 7760 ribosomalprotein S3 (rpsC) ORF03881 7761 & 7762 ribosomal protein L22 (rplV)ORF03882 7763 & 7764 ribosomal protein S19 (rpsS) ORF03883 7765 & 7766ribosomal protein L2 (rplB) ORF03884 7767 & 7768 ribosomal protein L23(rplW) ORF03885 7769 & 7770 ribosomal protein L4-L1 family (rplD)ORF03886 7771 & 7772 ribosomal protein L3 (rplC) ORF03887 7773 & 7774ribosomal protein S10 (rpsJ) ORF03888 7775 & 7776 PioO protein (PinOprotein) ORF03889 7777 & 7778 Probable general secretion pathway proteinA (GSP) ORF03890 7779 & 7780 general secretion pathway protein C (gspC)ORF03891 7781 & 7782 General secretion pathway protein D precursor(Pullulanase secretionenvelope pulD) (GSP) ORF03892 7783 & 7784 Generalsecretion pathway protein E (Type II traffic warden ATPase)(Choleratoxin secretion protein epsE) (GSP) ORF03893 7785 & 7786 generalsecretion pathway protein F (gspF) ORF03894 7787 & 7788 generalsecretion pathway protein G (gspG) ORF03895 7789 & 7790 Generalsecretion pathway protein H precursor (Pullulanase secretionproteinpulH) (GSP) ORF03896 7791 & 7792 outer membrane secretion protein V(GSP) ORF03897 7793 & 7794 general secretion pathway protein J (gspJ)ORF03898 7795 & 7796 General secretion pathway protein K (gspK) ORF038997797 & 7798 general secretion pathway protein L (gspL) ORF03900 7799 &7800 Putative general secretion pathway protein M (GSP) ORF03901 7801 &7802 Type 4 prepilin-like proteins leader peptide processing enzyme(GSP) [3.4.23.43] ORF03902 7803 & 7804 bacterioferritin (bfr) ORF039037805 & 7806 Bacterioferritin-associated ferredoxin-related proteinORF03904 7807 & 7808 chitinase [3.2.1.14] ORF03905 7809 & 7810translation elongation factor Tu (tuf) ORF03906 7811 & 7812 translationelongation factor G (fusA) ORF03907 7813 & 7814 ribosomal protein S7(rpsG) ORF03908 7815 & 7816 ribosomal protein S12 (rpsL) ORF03909 7817 &7818 DsrH protein ORF03910 7819 & 7820 Uncharacterized protein involvedin the oxidation of ORF03911 7821 & 7822 Uncharacterized conservedprotein involved in intracellular sulfur ORF03912 7823 & 7824 conservedhypothetical protein ORF03913 7825 & 7826 FKBP-type peptidyl-prolylcis-trans isomerase fkpA precursor(EC 5.2.1.8) (PPlase) (Rotamase)(rotamase) [5.2.1.8] ORF03914 7827 & 7828 SlyX protein (slyX) ORF039157829 & 7830 FKBP-type peptidyl-prolyl cis-trans isomerase slyD(PPlase)(Rotamase) (Histidine- rich protein) (WHP) (rotamase) [5.2.1.8] ORF039167831 & 7832 conserved hypothetical protein ORF03917 7833 & 7834Glutathione-regulated potassium-efflux system protein kefB(K(+)-H(+)antiporter) (NEM-activatable K(+)—H(+) antiporter) ORF039187835 & 7836 Glutathione-regulated potassium-efflux system ancillaryprotein kefG [1.6.99.2] ORF03919 7837 & 7838 ABC transporter ATP-bindingprotein ORF03920 7839 & 7840 Predicted hydrolase of thealpha-beta-hydrolase fold ORF03921 7841 & 7842 Hypothetical UPF0270protein yheU-related protein ORF03922 7843 & 7844 phosphoribulokinase[2.7.1.19] ORF03923 7845 & 7846 no known function ORF03924 7847 & 7848Catabolite gene activator (cAMP receptor protein)(cAMP-regulatoryprotein) (crp) ORF03925 7849 & 7850 integral membraneprotein, YccS-YhfK family subfamily ORF03926 7851 & 7852 Acetylornithineaminotransferase (argD) [2.6.1.11] ORF03927 7853 & 7854Para-aminobenzoate synthase glutamine amidotransferase component II[6.3.5.8] ORF03928 7855 & 7856 fic, putative ORF03929 7857 & 7858Peptidyl-prolyl cis-trans isomerase A precursor (PPlaseA) (Rotamase A)(Cyclophilin A) (utu) [5.2.1.8] ORF03930 7859 & 7860 hypotheticalprotein ORF03931 7861 & 7862 TsgA protein ORF03932 7863 & 7864 nitritereductase [NAD(P)H], large subunit (nirB) [1.7.1.4] ORF03933 7865 & 7866nitrite reductase [NAD(P)H], small subunit (nirD) [1.7.1.4] ORF039347867 & 7868 Potential nitrite transporter (formate) ORF03935 7869 & 7870Siroheme synthase ORF03936 7871 & 7872 lipoprotein, putative ORF039377873 & 7874 unnamed protein product ORF03938 7875 & 7876 membraneprotein, putative ORF03939 7877 & 7878 conserved hypothetical proteinORF03940 7879 & 7880 parathion hydrolase, putative ORF03941 7881 & 7882phosphopentomutase ORF03942 7883 & 7884 Alanine racemase, N-terminaldomain family ORF03943 7885 & 7886 unnamed protein product ORF03944 7887& 7888 conserved hypothetical protein ORF03945 7889 & 7890tryptophanyl-tRNA synthetase (trpS) [6.1.1.2] ORF03946 7891 & 7892phosphoglycolate phosphatase, bacterial (gph) [3.1.3.18] ORF03947 7893 &7894 hypothetical protein ORF03948 7895 & 7896 Phosphoglycolatephosphatase (PGP) [3.1.3.18] ORF03949 7897 & 7898 ribulose-phosphate3-epimerase (rpe) [5.1.3.1] ORF03950 7899 & 7900 DNA adenine methylase(dam) [2.1.1.72] ORF03951 7901 & 7902 DamX protein (1989) ORF03952 7903& 7904 3-dehydroquinate synthase (aroB) [4.2.3.4] ORF03953 7905 & 7906shikimate kinase I ORF03954 7907 & 7908 Protein transport protein hofQprecursor (Tfp) ORF03955 7909 & 7910 conserved hypothetical proteinORF03956 7911 & 7912 conserved hypothetical protein ORF03957 7913 & 7914Fimbrial assembly protein (PilN) superfamily ORF03958 7915 & 7916conserved hypothetical protein ORF03959 7917 & 7918 Penicillin-bindingprotein 1A(PBP1a) [2.4.2.—] ORF03960 7919 & 7920 ADP compounds hydrolasenudE [3.6.1.—] ORF03961 7921 & 7922 Intracellular growth attenuatorprotein igaA ORF03962 7923 & 7924 HAD-superfamily hydrolase, subfamilyIA, variant 3 protein family ORF03963 7925 & 7926 Heat shock protein 15(HSP15) (HSP15) ORF03964 7927 & 7928 33 kDa chaperonin (Heat shockprotein 33) (HSP33) (HSP33) ORF03965 7929 & 7930 membrane protein,putative ORF03966 7931 & 7932 phosphoenolpyruvate carboxykinase (ATP)(pckA) [4.1.1.49] ORF03967 7933 & 7934 Osmolarity sensor protein envZ[2.7.3.—] ORF03968 7935 & 7936 response regulator (sensor, EnvZ)affecting transcription of ompC and ompF: outer membrane proteinsynthesis ORF03969 7937 & 7938 transcription elongation factor GreB(greB) ORF03970 7939 & 7940 Protein yhgF ORF03971 7941 & 7942 ferrousiron transport protein B ORF03972 7943 & 7944 ferrous iron transportprotein B (feoB) ORF03973 7945 & 7946 conserved hypothetical proteinORF03974 7947 & 7948 transposase ORF03975 7949 & 7950 hypotheticalprotein ORF03976 7951 & 7952 bioH protein (bioH) ORF03977 7953 & 7954hypothetical protein ORF03978 7955 & 7956 predictedamidophosphoribosyltransferase ORF03979 7957 & 7958 Protein yhgIORF03980 7959 & 7960 High-affinity gluconate transporter (Gluconatepermease) (Gnt-Isystem) ORF03981 7961 & 7962 4-alpha-glucanotransferase(malQ) [2.4.1.25] ORF03982 7963 & 7964 Maltodextrin phosphorylase[2.4.1.1] ORF03983 7965 & 7966 transcriptional regulator, LuxR family,putative ORF03984 7967 & 7968 RNA 3′-terminal phosphate cyclase (rtcA)[6.5.1.4] ORF03985 7969 & 7970 Protein rtcB ORF03986 7971 & 7972Glycerol-3-phosphate regulon repressor ORF03987 7973 & 7974Transcriptional Regulatory protein rtcR ORF03988 7975 & 7976 Rhomboidfamily protein, putative ORF03989 7977 & 7978 unnamed protein product;glpE polypeptide (AA 1-131) ORF03990 7979 & 7980 Aerobicglycerol-3-phosphate dehydrogenase (aerobic) ORF03991 7981 & 7982conserved hypothetical protein ORF03992 7983 & 7984 conservedhypothetical protein ORF03993 7985 & 7986 conserved hypothetical proteinORF03994 7987 & 7988 mannose binding protein FimH precursor ORF039957989 & 7990 fimbrial chaperone ORF03996 7991 & 7992 Putative minorfimbrial subunit precursor ORF03997 7993 & 7994 Putative minor fimbrialsubunit precursor ORF03998 7995 & 7996 F1C fimbrial usher ORF03999 7997& 7998 Chaperone protein fimC precursor ORF04000 7999 & 8000 Long polarfimbria protein A precursor (pilin) ORF04001 8001 & 8002 Glycogenphosphorylase [2.4.1.1] ORF04002 8003 & 8004 Glycogen synthase (Starch[bacterial glycogen]synthase) [2.4.1.21] ORF04003 8005 & 8006glucose-1-phosphate adenylyltransferase (glgC) [2.7.7.27] ORF04004 8007& 8008 glycogen debranching enzyme GlgX (glgX) [3.2.1.—] ORF04005 8009 &8010 1,4-alpha-glucan branching enzyme (glgB) [2.4.1.18] ORF04006 8011 &8012 aspartate-semialdehyde dehydrogenase (asd) [1.2.1.11] ORF04007 8013& 8014 membrane protein, putative ORF04008 8015 & 8016 gluconatepermease, putative ORF04009 8017 & 8018 thermoresistant gluconokinase(gluconate kinase 2) ORF04010 8019 & 8020 HTH-type transcriptionalregulator gntR (Gluconate utilization systemGNT-I transcriptionalrepressor) (gnt) ORF04011 8021 & 8022 Protein yhhW ORF04012 8023 & 8024Putative oxidoreductase yhhX [1.—.—.—] ORF04013 8025 & 8026 conservedhypothetical protein ORF04014 8027 & 8028 acetyltransferase, GNATfamily, putative [2.3.1.—] ORF04015 8029 & 8030 unnamed protein product;Similar to Hcp protein ORF04016 8031 & 8032 conserved hypotheticalprotein ORF04017 8033 & 8034 gamma-glutamyltransferase (ggt) [2.3.2.2]ORF04018 8035 & 8036 glycerophosphodiester phosphodiesterase [3.1.4.46]ORF04019 8037 & 8038 CG9973-PA ORF04020 8039 & 8040SN-glycerol-3-phosphate transport ATP-binding protein ugpC ORF04021 8041& 8042 SN-glycerol-3-phosphate transport system permease protein ugpE(membrane) ORF04022 8043 & 8044 glycerol-3-phosphate ABC transporter,permease protein (membrane) ORF04023 8045 & 8046Glycerol-3-phosphate-binding periplasmic protein precursor ORF04024 8047& 8048 ABC transporter domain protein ORF04025 8049 & 8050 High-affinitybranched-chain amino acid transport ATP-binding proteinlivG (LIV-Iprotein G) (AJ272047) ORF04026 8051 & 8052 High-affinity branched-chainamino acid transport system permeaseprotein livM (LIV-I protein M)ORF04027 8053 & 8054 High-affinity branched-chain amino acid transportsystem permease protein livH ORF04028 8055 & 8056 Leucine-specificbinding protein precursor ORF04029 8057 & 8058 acetyltransferase, GNATfamily family ORF04030 8059 & 8060 Leu-Ile-Val-binding protein precursorORF04031 8061 & 8062 PTS system, mannose-specific enzyme II, ABcomponent (EIII-MAN) [2.7.1.69] ORF04032 8063 & 8064 PTS system,mannose-specific IIAB components, putative [2.7.1.69] ORF04033 8065 &8066 PTS system, IIC component (PTS) ORF04034 8067 & 8068mannose-specific PTS enzyme IID ORF04035 8069 & 8070D-3-phosphoglycerate dehydrogenase (serA) [1.1.1.95] ORF04036 8071 &8072 dihydrodipicolinate synthase (dapA) [4.2.1.52] ORF04037 8073 & 8074alternative sigma factor RpoH (rpoH) ORF04038 8075 & 8076 putativeprotein insertion permease FtsX (ftsX) ORF04039 8077 & 8078 Celldivision ATP-binding protein ftsE ORF04040 8079 & 8080 Cell divisionprotein ftsY ORF04041 8081 & 8082 methyltransferase, putative ORF040428083 & 8084 overlaps previous ORF, probably uses downstream startORF04043 8085 & 8086 conserved hypothetical protein ORF04044 8087 & 8088membrane protein, putative ORF04045 8089 & 8090 Lead, cadmium, zinc andmercury transporting ATPase [3.6.3.3] ORF04046 8091 & 8092 SirA proteinORF04047 8093 & 8094 membrane protein (o221) ORF04048 8095 & 8096 DcrBprotein precursor ORF04049 8097 & 8098 Hypothetical UPF0226 protein yhhSORF04050 8099 & 8100 Domain of unknown function, putative ORF04051 8101& 8102 4′-phosphopantetheinyl transferase acpT [2.7.8.—] ORF04052 8103 &8104 nickel ABC transporter, periplasmic nickel-binding protein (nikA)ORF04053 8105 & 8106 Nickel transport system permease protein nikB(permease) ORF04054 8107 & 8108 dipeptide ABC transporter, permeaseprotein, putative ORF04055 8109 & 8110 Nickel transport ATP-bindingprotein nikD ORF04056 8111 & 8112 Nickel transport ATP-binding proteinnikE ORF04057 8113 & 8114 Nickel responsive regulator ORF04058 8115 &8116 transcriptional regulator, GntR family, putative ORF04059 8117 &8118 PTS system IIA component, probable , putative ORF04060 8119 & 8120PTS system, galactitol-specific enzyme II, B component [2.7.1.69]ORF04061 8121 & 8122 PTS system, sorbitol-specific IIC componentORF04062 8123 & 8124 probable xylulose kinase Z4878 [2.7.1.30] ORF040638125 & 8126 phosphotransferase system HPr enzyme (PTS) ORF04064 8127 &8128 Tagatose-bisphosphate aldolase GatY [4.1.2.—] ORF04065 8129 & 8130ABC-2 type transporter family ORF04066 8131 & 8132 ribosome-associatedATPase, ATP-binding domain (N-terminal) ORF04067 8133 & 8134 Type Iantifreeze protein: HlyD family secretion protein ORF04068 8135 & 8136membrane protein, putative ORF04069 8137 & 8138 conserved hypotheticalprotein TIGR00275 ORF04070 8139 & 8140 Low-affinity inorganic phosphatetransporter 1 ORF04071 8141 & 8142 Universal stress protein B ORF040728143 & 8144 universal stress protein A (UspA) ORF04073 8145 & 8146hypothetical protein ORF04074 8147 & 8148 Hypothetical transporter yhiPORF04075 8149 & 8150 gtg start, alternate starts possible ORF04076 8151& 8152 Oligopeptidase A ORF04077 8153 & 8154 Protein of unknown function(DUF519) superfamily ORF04078 8155 & 8156 glutathione-disulfidereductase (gor) [1.8.1.7] ORF04079 8157 & 8158 conserved hypotheticalprotein ORF04080 8159 & 8160 Arsenate reductase [1.20.4.1] ORF04081 8161& 8162 outer membrane protein induced after carbon starvation ORF040828163 & 8164 Hypothetical transcriptional regulator yhiF ORF04083 8165 &8166 Hemin transport protein hmuS ORF04084 8167 & 8168 outer membranehemin receptor ChuA ORF04085 8169 & 8170 conserved hypothetical proteinORF04086 8171 & 8172 Putative Periplasmic binding protein ORF04087 8173& 8174 HugW ORF04088 8175 & 8176 HuvX protein (fragment) ORF04089 8177 &8178 conserved hypothetical protein ORF04090 8179 & 8180 hemin ABCtransporter permease protein ORF04091 8181 & 8182 hemin transport systemATP-binding protein ORF04092 8183 & 8184 cation transport ATPase yqgGORF04093 8185 & 8186 protein hdeB precursor ORF04094 8187 & 8188 ProteinhdeA precursor, putative ORF04095 8189 & 8190 HdeD protein ORF04096 8191& 8192 conserved hypothetical protein ORF04097 8193 & 8194 multidrugefflux pump SdeX ORF04098 8195 & 8196 RND family, acridine-multidrugefflux pump ORF04099 8197 & 8198 HTH-type transcriptional regulator appY(M5 polypeptide) ORF04100 8199 & 8200 Transcriptional regulator gadXORF04101 8201 & 8202 glutamate decarboxylase [4.1.1.15] ORF04102 8203 &8204 cytochrome c551 peroxidase ORF04103 8205 & 8206 Trehalase [3.2.1.28] ORF04104 8207 & 8208 virulence regulator ORF04105 8209 & 8210transcriptional regulator ORF04106 8211 & 8212 ribonuclease, putativeORF04107 8213 & 8214 metabolite-proton symporter ORF04108 8215 & 8216Uncharacterized protein involved in outer membrane biogenesis ORF041098217 & 8218 EAL domain, putative ORF04110 8219 & 82202-dehydro-3-deoxygluconokinase ORF04111 8221 & 8222 Protein yhjJprecursor [3.4.99.—] ORF04112 8223 & 8224 Aerobic C4-dicarboxylatetransport protein ORF04113 8225 & 8226 Protein yhjK ORF04114 8227 & 8228Cellulose synthase operon protein C ORF04115 8229 & 8230 Endoglucanaseprecursor ORF04116 8231 & 8232 Cyclic di-GMP binding protein precursorORF04117 8233 & 8234 Cellulose synthase catalytic subunit [UDP-forming]ORF04118 8235 & 8236 YhjQ protein family ORF04119 8237 & 8238 conservedhypothetical protein ORF04120 8239 & 8240 conserved hypothetical proteinORF04121 8241 & 8242 conserved hypothetical protein ORF04122 8243 & 8244conserved hypothetical protein ORF04123 8245 & 8246 conservedhypothetical protein ORF04124 8247 & 8248 Hypothetical transport proteinyhjV ORF04125 8249 & 8250 peptide ABC transporter, ATP-binding protein(atp_bind) ORF04126 8251 & 8252 Dipeptide transport ATP-binding proteindppD (atp_bind) ORF04127 8253 & 8254 Dipeptide transport system permeaseprotein dppC (membrane) ORF04128 8255 & 8256 Dipeptide transport systempermease protein dppB (permease) ORF04129 8257 & 8258 Periplasmicdipeptide transport protein precursor (DBP) ORF04130 8259 & 8260conserved hypothetical protein ORF04131 8261 & 8262 Membrane-proteinyhjW ORF04132 8263 & 8264 putative resistance protein ORF04133 8265 &8266 conserved hypothetical protein ORF04134 8267 & 8268DNA-3-methyladenine glycosylase I [3.2.2.20] ORF04135 8269 & 8270 Biotinsulfoxide reductase ORF04136 8271 & 8272 Hypothetical acetyltransferaseyiaC (GNAT) [2.3.1.—] ORF04137 8273 & 8274 probable outer membraneprotein yiaD ORF04138 8275 & 8276 2-ketogluconate reductase [1.1.1.215]ORF04139 8277 & 8278 lipoprotein, putative ORF04140 8279 & 8280 PutativeHTH-type transcriptional regulator yiaG ORF04141 8281 & 8282 coldshock-like protein ORF04142 8283 & 8284 probable membrane permeabilityaltering protein Z4982 -related protein ORF04143 8285 & 8286 glycyl-tRNAsynthetase, beta subunit (glyS) [6.1.1.14] ORF04144 8287 & 8288glycyl-tRNA synthetase, alpha subunit (glyQ) [6.1.1.14] ORF04145 8289 &8290 conserved hypothetical protein ORF04146 8291 & 8292 Acyltransferasefamily, putative ORF04147 8293 & 8294 yiaA-B two helix domain familyORF04148 8295 & 8296 yiaA-B two helix domain family ORF04149 8297 & 8298xylulokinase (xylB) [2.7.1. 17] ORF04150 8299 & 8300 xylose isomerase(xylA) [5.3.1.5] ORF04151 8301 & 8302 D-xylose ABC transporter,periplasmic-D xylose binding protein ORF04152 8303 & 8304 sugar ABCtransporter, ATP-binding protein (xylG) ORF04153 8305 & 8306 sugar ABCtransporter, permease protein ORF04154 8307 & 8308 Xylose operonregulatory protein (xylR) ORF04155 8309 & 8310 BAX protein ORF04156 8311& 8312 Alpha-amylase precursor [3.2.1.1] ORF04157 8313 & 8314Valine--pyruvate aminotransferase ORF04158 8315 & 8316 Electrontransport protein hydN ORF04159 8317 & 8318 lclR family transcriptionalrepressor ORF04160 8319 & 8320 2-keto reductase [1.1.1.—] ORF04161 8321& 8322 YiaL ORF04162 8323 & 8324 YiaX1 ORF04163 8325 & 8326 TRAPtransporter, DctQ-like membrane protein superfamily ORF04164 8327 & 8328C4-dicarboxylate transport system permease large protein (DctM) ORF041658329 & 8330 DctP protein (AP001509) ORF04166 8331 & 8332 sugar kinase,FGGY family, putative [2.7.1.53] ORF04167 8333 & 8334hexulose-6-phosphate synthase (humps) [4.1.2.—] ORF04168 8335 & 8336hexulose-6-phosphate isomerase, putative ORF04169 8337 & 8338L-ribulose-5-phosphate4-epimerase (araD) [5.1.3.4] ORF04170 8339 & 8340aldehyde dehydrogenase family protein (AF029733) ORF04171 8341 & 8342Fic protein family family ORF04172 8343 & 8344 alcohol dehydrogenase,class IV [1.1.1.1] ORF04173 8345 & 8346 selenocysteine-specifictranslation elongation factor (selB) ORF04174 8347 & 8348 L-seryl-tRNAselenium transferase (selA) [2.9.1.1] ORF04175 8349 & 8350 HypotheticalGST-like protein yibF ORF04176 8351 & 8352 multidrug resistance effluxpump ORF04177 8353 & 8354 GTPase ORF04178 8355 & 8356 PTS system,mannitol-specific enzyme IIABC components [2.7.1.69] ORF04179 8357 &8358 Mannitol-1-phosphate 5-dehydrogenase [1.1.1.17] ORF04180 8359 &8360 Mannitol operon repressor (Mannitol repressor protein) ORF041818361 & 8362 conserved hypothetical protein ORF04182 8363 & 8364conserved hypothetical protein ORF04183 8365 & 8366 conservedhypothetical protein ORF04184 8367 & 8368 surface protein (partial)ORF04185 8369 & 8370 L-lactate permease ORF04186 8371 & 8372transcriptional regulator ORF04187 8373 & 8374 L-lactate dehydrogenase(lctD) [1.1.2.3] ORF04188 8375 & 8376 RNA methyltransferase, TrmHfamily, group 2 ORF04189 8377 & 8378 Serine acetyltransferase (SAT)(SAT) [2.3.1.30] ORF04190 8379 & 8380 Glycerol-3-phosphate dehydrogenase[NAD(P)+] (NAD(P)H-dependent glycerol-3- phosphate dehydrogenase) (gpsA)[1.1.1.94] ORF04191 8381 & 8382 protein-export protein SecB (secB)ORF04192 8383 & 8384 glutaredoxin 3 (grxC) ORF04193 8385 & 8386Rhodanese-like domain protein ORF04194 8387 & 83882,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)[5.4.2.1] ORF04195 8389 & 8390 Membrane-bound metallopeptidase ORF041968391 & 8392 glycosyl transferase, group 2 family protein, putative[2.—.—.—] ORF04197 8393 & 8394 yibQ gene product ORF04198 8395 & 8396L-threonine 3-dehydrogenase (tdh) [1.1.1.103] ORF04199 8397 & 83982-amino-3-ketobutyrate coenzyme A ligase (kbl) [2.3.1.29] ORF04200 8399& 8400 ADP-L-glycero-D-manno-heptose-6-epimerase (rfaD) [5.1.3.20]ORF04201 8401 & 8402 lipopolysaccharide heptosyltransferase II (rfaF)ORF04202 8403 & 8404 lipopolysaccharide heptosyltransferase I (rfaC)[2.—.—.—] ORF04203 8405 & 8406 Lipid A-core, surface polymer ligaseORF04204 8407 & 8408 beta-1,3-glucosyltransferase [2.4.1.166] ORF042058409 & 8410 UDP-galactose: (galactosyl)LPSalpha-1,2-galactosyltransferase (glucosyl) [2.4.1.58] ORF04206 8411 &8412 heptosyl-II-kinase ORF04207 8413 & 8414 UDP-galactose: (glucosyl)[2.4.1.58] ORF04208 8415 & 8416 UDP-glucose: (glucosyl) [2.4.1.44]ORF04209 8417 & 8418 Lipopolysaccharide core biosynthesis protein rfaPORF04210 8419 & 8420 UDP-glucose: (heptosyl) [2.4.1.—] ORF04211 8421 &8422 lipopolysaccharide heptosyltransferase III, putative ORF04212 8423& 8424 3-deoxy-D-manno-octulosonic-acid transferase (KDOtransferase)(KDO) [2.—.—.—] ORF04213 8425 & 8426 pantetheine-phosphateadenylyltransferase (coaD) [2.7.7.3] ORF04214 8427 & 8428formamidopyrimidine-DNAglycosylase (mutM) [3.2.2.23] ORF04215 8429 &8430 ribosomal protein L33 (rpmG) ORF04216 8431 & 8432 RpmB protein(rpL28) ORF04217 8433 & 8434 DNA repair protein ORF04218 8435 & 8436phosphopantothenoylcysteine decarboxylase-phosphopantothenate--cysteineligase (coaBC) [4. 1.1. 36 6.3.2.5] ORF04219 8437 & 8438 Deoxyuridine5′-triphosphate nucleotidohydrolase (dut) [3.6.1.23] ORF04220 8439 &8440 Ttk protein ORF04221 8441 & 8442 orotate phosphoribosyltransferase(pyrE) [2.4.2.10] ORF04222 8443 & 8444 ribonuclease PH (rph) [2.7.7.56]ORF04223 8445 & 8446 conserved hypothetical protein TIGR00255 ORF042248447 & 8448 hypothetical protein ORF04225 8449 & 8450DNA-damage-inducible protein D ORF04226 8451 & 8452 predicted membraneprotein ORF04227 8453 & 8454 Hypothetical DNA ligase-like protein yicFORF04228 8455 & 8456 conserved hypothetical protein ORF04229 8457 & 8458guanylate kinase ORF04230 8459 & 8460 DNA-directed RNA polymerase omegachain (RNAP omegasubunit) (Transcriptase omega chain) (RNA polymeraseomega subunit) (rpoZ) [2.7.7.6] ORF04231 8461 & 8462Guanosine-3,5-bis(Diphosphate) 3-pyrophosphohydrolase (ppGpp) ORF042328463 & 8464 tRNA(Guanosine-2′-O-)-methyltransferase (Gm18) [2.1.1.34]ORF04233 8465 & 8466 ATP-dependent DNA helicase RecG (recG) [3.6.1.—]ORF04234 8467 & 8468 sodium-glutamate symporter (gltS) ORF04235 8469 &8470 xanthine-uracil permease family protein ORF04236 8471 & 8472possible exported protein ORF04237 8473 & 8474 Protein of unknownfunction (DUF1498) superfamily ORF04238 8475 & 8476 possible NAGC-liketranscriptional regulator ORF04239 8477 & 8478 fructose-bisphosphatealdolase, class II family [4.1.2.13] ORF04240 8479 & 8480fructose-bisphosphate aldolase, class II family [4.1.2.—] ORF04241 8481& 8482 PTS system, IIbc component (PTS) [2.7.1.69] ORF04242 8483 & 8484PTS system, fructose-like-2 IIB component 1 ORF04243 8485 & 8486 PTSsystem, Ella component (PTS) [2.7.1.69] ORF04244 8487 & 8488transcription antiterminator BgIG family protein,, putative ORF042458489 & 8490 f772 [3.2. 1.20] ORF04246 8491 & 8492 similar to melibiosecarrier protein ORF04247 8493 & 8494 RhuM (partial) ORF04248 8495 & 8496membrane protein ORF04249 8497 & 8498 Lipoprotein-28 precursor (hlpA)ORF04250 8499 & 8500 conserved hypothetical protein ORF04251 8501 & 8502putative transport protein ORF04252 8503 & 8504 Protein of unknownfunction (DUF1198) superfamily ORF04253 8505 & 8506 xanthine-uracilpermease family protein subfamily ORF04254 8507 & 8508 adenine deaminase(ade) [3.5.4.2] ORF04255 8509 & 8510 Hexose phosphate transport proteinORF04256 8511 & 8512 Regulatory protein uhpC ORF04257 8513 & 8514 Sensorprotein uhpB ORF04258 8515 & 8516 hypothetical protein ORF04259 8517 &8518 response regulator, positive activator of uhpT transcriptionORF04260 8519 & 8520 acetolactate synthase, small subunit, putative[2.2.1.6] ORF04261 8521 & 8522 acetolactate synthase, large subunit,biosynthetic type (ilvB) [2.2.1.6] ORF04262 8523 & 8524 Multidrugresistance protein D ORF04263 8525 & 8526 f165 [1.—.—.—] ORF04264 8527 &8528 conserved hypothetical protein ORF04265 8529 & 8530 Predictedmembrane protein ORF04266 8531 & 8532 probable sulfatase yidJ [3.1.6.—]ORF04267 8533 & 8534 Putative sulfatase yidJ [3.1.6.—] ORF04268 8535 &8536 Na+-myo-inositol cotransporter ORF04269 8537 & 8538 msm operonregulatory protein , putative ORF04270 8539 & 8540 potassium uptakeprotein TrkA, putative ORF04271 8541 & 8542 heat shock protein ORF042728543 & 8544 heat shock protein ORF04273 8545 & 8546 hypothetical proteinORF04274 8547 & 8548 o135 protein ORF04275 8549 & 8550 f416 ORF042768551 & 8552 MFS transporter, phthalate permease family ORF04277 8553 &8554 conserved hypothetical protein ORF04278 8555 & 8556 mandelateracemase-muconate lactonizing enzyme family protein (partial) [4.2.1.6]ORF04279 8557 & 8558 4-hydroxy-2-oxoglutaratealdolase-2-dehydro-3-deoxyphosphogluconate [4.1.2.21] ORF04280 8559 &8560 2-dehydro-3-deoxygalactonate kinase [2.7.1.58] ORF04281 8561 & 8562transcriptional repressor for galactonate utilization (GntR family)(AF096293) ORF04282 8563 & 8564 probable replicase Z5187 ORF04283 8565 &8566 Cof family protein ORF04284 8567 & 8568 Bacterial protein ofunknown function (DUF937) family ORF04285 8569 & 8570 DNA gyrase, Bsubunit (gyrB) [5.99.1.3] ORF04286 8571 & 8572 DNA replication andrepair protein recF [similarity] ORF04287 8573 & 8574 DNA polymeraseIII, beta subunit (dnaN) [2.7.7.7] ORF04288 8575 & 8576 chromosomalreplication initiator protein DnaA (dnaA) ORF04289 8577 & 8578 ribosomalprotein L34 (rpmH) ORF04290 8579 & 8580 ribonuclease P protein component(rnpA) [3.1.26.5] ORF04291 8581 & 8582 conserved hypothetical proteinTIGR00278 ORF04292 8583 & 8584 Inner membrane protein oxaA (IMP)ORF04293 8585 & 8586 tRNA modification GTPase TrmE (trmE) ORF04294 8587& 8588 tryptophanase (TNase) [4.1.99.1] ORF04295 8589 & 8590 Lowaffinity tryptophan permease ORF04296 8591 & 8592 similar to drugresistance translocases ORF04297 8593 & 8594 transcriptional regulator,LysR family ORF04298 8595 & 8596 hypothetical protein ORF04299 8597 &8598 o252 ORF04300 8599 & 8600 YieF (putative) ORF04301 8601 & 8602 f445ORF04302 8603 & 8604 haloacid dehalogenase-like hydrolase, putativeORF04303 8605 & 8606 conserved hypothetical protein ORF04304 8607 & 8608pyrimidine-specific nucleoside hydrolase [3.5.99.6] ORF04305 8609 & 8610Putative esterase family, putative ORF04306 8611 & 8612 putativexylanase ORF04307 8613 & 8614 f538 ORF04308 8615 & 86166-phospho-beta-glucosidase bglB [3.2.1.86] ORF04309 8617 & 8618 PTSsystem, beta-glucoside-specific IIABC component ORF04310 8619 & 8620Cryptic beta-glucoside bgl operon Antiterminator ORF04311 8621 & 8622phosphate transport system regulatory protein PhoU (phoU) ORF04312 8623& 8624 phosphate ABC transporter, ATP-binding protein (pstB) [3.6.3.27]ORF04313 8625 & 8626 phosphate ABC transporter, permease protein PstA(pstA) ORF04314 8627 & 8628 phosphate ABC transporter, permease proteinPstC (pstC) ORF04315 8629 & 8630 phosphate ABC transporter,phosphate-binding protein (pstS) ORF04316 8631 & 8632glucosamine--fructose-6-phosphate aminotransferase, isomerizing (glmS)[2.6.1.16] ORF04317 8633 & 8634 UDP-N-acetylglucosaminepyrophosphorylase (glmU) [2.7.7.23] ORF04318 8635 & 8636 ATP synthaseF1, epsilon subunit (atpC) [3.6.3.14] ORF04319 8637 & 8638 ATP synthaseF1, beta subunit (atpD) [3.6.3.14] ORF04320 8639 & 8640 ATP synthase F1,gamma subunit (atpG) [3.6.3.14] ORF04321 8641 & 8642 ATP synthase F1,alpha subunit (atpA) [3.6.3.14] ORF04322 8643 & 8644 ATP synthase F1,delta subunit (atpH) [3.6.3.14] ORF04323 8645 & 8646 ATP synthase F0, Bsubunit (atpF) [3.6.3.14] ORF04324 8647 & 8648 conserved hypotheticalprotein ORF04325 8649 & 8650 ATP synthase F0, C subunit [3.6.3.14]ORF04326 8651 & 8652 ATP synthase F0, A subunit (atpB) [3.6.3.14]ORF04327 8653 & 8654 ATP synthase F0, I subunit (atpI) [3.6.3.14]ORF04328 8655 & 8656 methyltransferase GidB (gidB) [2.1.—.—] ORF043298657 & 8658 glucose-inhibited division protein A (gidA) ORF04330 8659 &8660 Protein mioC (mioC) ORF04331 8661 & 8662 transcriptional regulator,AsnC Family (asnC) ORF04332 8663 & 8664 aspartate-ammonia ligase (asnA)[6.3.1.1] ORF04333 8665 & 8666 conserved hypothetical protein ORF043348667 & 8668 unnamed protein product ORF04335 8669 & 8670 potassiumuptake protein (kup) ORF04336 8671 & 8672 high affinity ribose transportprotein rbsD ORF04337 8673 & 8674 ribose ABC transporter, ATP-bindingprotein (atp_bind) ORF04338 8675 & 8676 ribose ABC transporter, permeaseprotein (rbsC) ORF04339 8677 & 8678 ribose ABC transporter, periplasmicD-ribose-binding protein ORF04340 8679 & 8680 ribokinase (rbsK)[2.7.1.15] ORF04341 8681 & 8682 drug resistance transporter, EmrB-QacAfamily ORF04342 8683 & 8684 regulator for rbs operon ORF04343 8685 &8686 ProP protein ORF04344 8687 & 8688 yieP gene product (AF096293)ORF04345 8689 & 8690 hypothetical protein ORF04346 8691 & 8692Transcription regulator ORF04347 8693 & 8694 o137 (YIFE) ORF04348 8695 &8696 Mg chelatase-related protein ORF04349 8697 & 8698 hypotheticalprotein ORF04350 8699 & 8700 acetolactate synthase, large subunit,biosynthetic type (ilvB) [2.2.1.6] ORF04351 8701 & 8702 acetolactatesynthase II small chain (ALS-II) [4.1.3.18] ORF04352 8703 & 8704branched-chain amino acid aminotransferase (ilvE) [2.6.1.42] ORF043538705 & 8706 dihydroxy-acid dehydratase (ilvD) [4.2.1.9] ORF04354 8707 &8708 threonine ammonia-lyase, biosynthetic (ilvA) [4.3.1.19] ORF043558709 & 8710 transcriptional regulator, LysR family, putative ORF043568711 & 8712 Ketol-acid reductoisomerase (ILVC) [1.1.1.86] ORF04357 8713& 8714 peptidylprolyl isomerase (SPP39159) [5.2.1.8] ORF04358 8715 &8716 ATP-dependent DNAhelicase Rep (rep) [3.6.1.—] ORF04359 8717 & 8718Guanosine-5′-triphosphate,3′-diphosphatepyrophosphatase [3.6.1.40]ORF04360 8719 & 8720 rhIB protein (RHLB) [3.6.1.—] ORF04361 8721 & 8722thioredoxin 1 ORF04362 8723 & 8724 transcription termination factor Rho(rho) ORF04363 8725 & 8726 conserved hypothetical protein ORF04364 8727& 8728 undecaprenyl-phosphate alpha-N-acetylglucosaminyl1-phosphatetransferase (wecA) [2.7.8.—] ORF04365 8729 & 8730Lipopolysaccharide biosynthesis protein wzzE ORF04366 8731 & 8732UDP-N-acetylglucosamine 2-epimerase [5.1.3.14] ORF04367 8733 & 8734UDP-N-acetyl-D-mannosaminede hydrogenase (ECA) [1.1.1.—] ORF04368 8735 &8736 dTDP-glucose 4,6-dehydratase (rfbB) [4.2.1.46] ORF04369 8737 & 8738glucose-1-phosphate thymidylyltransferase (rfbA) [2.7.7.24] ORF043708739 & 8740 TDP-D-fucosamine acetyltransferase (wecD) ORF04371 8741 &8742 Lipopolysaccharide biosynthesis protein rffA (WECE) [4.2.1.—]ORF04372 8743 & 8744 WzxE protein (WZXE) ORF04373 8745 & 8746TDP-Fuc4NAc: lipid II Fuc4Nac transferase (wecF) [2.4.1.—] ORF04374 8747& 8748 ECA biosynthesis protein WzyE (wzyE) ORF04375 8749 & 8750possibly rffM (ECA) [2.4.1.—] ORF04376 8751 & 8752 hypothetical proteinORF04377 8753 & 8754 similar to several amino acid transport proteins(o461) ORF04378 8755 & 8756 tyrosine recombinase XerC (xerC) ORF043798757 & 8758 o238 (YIGB) ORF04380 8759 & 8760 tyrosine recombinase XerC(xerC) ORF04381 8761 & 8762 o238 (YIGB) ORF04382 8763 & 8764arylsulfatase regulator ORF04383 8765 & 8766 Arylsulfatase [3.1.6.1]ORF04384 8767 & 8768 hemY protein (hemY) ORF04385 8769 & 8770uroporphyrinogen III methylase (HEMX) [2.1.1.107] ORF04386 8771 & 8772uroporphyrinogen-III synthase (hemD) [4.2.1.75] ORF04387 8773 & 8774porphobilinogen deaminase (hemC) [2.5.1.61] ORF04388 8775 & 8776adenylate cyclase, class-I (cyaA) [4.6.1.1] ORF04389 8777 & 8778 CyaYprotein (CYAY) ORF04390 8779 & 8780 possible exported protein ORF043918781 & 8782 lipoprotein, putative ORF04392 8783 & 8784 Hypotheticallipoprotein yifL precursor-related protein ORF04393 8785 & 8786diaminopimelate epimerase (dapF) [5.1.1.7] ORF04394 8787 & 8788 Proteinof unknown function, DUF484 superfamily ORF04395 8789 & 8790 tyrosinerecombinase XerC (xerC) ORF04396 8791 & 8792 o238 (YIGB) ORF04397 8793 &8794 DNA helicase II (uvrD) [3.6.1.—] ORF04398 8795 & 8796 Protein ofunknown function superfamily ORF04399 8797 & 8798 yigE protein ORF044008799 & 8800 hypothetical protein ORF04401 8801 & 8802 magnesium andcobalt transport protein CorA (corA) ORF04402 8803 & 8804Enterobacterial putative membrane protein (DUF943) superfamily ORF044038805 & 8806 conserved hypothetical protein ORF04404 8807 & 8808hypothetical protein ORF04405 8809 & 8810 rarD protein (rarD) ORF044068811 & 8812 f161 (YIGI) ORF04407 8813 & 8814 phospholipase A1 familyprotein, interruption [3.1.1.32] ORF04408 8815 & 8816 ATP-dependent DNAhelicase RecQ (recQ) [3.6.1.—] ORF04409 8817 & 8818 threonine effluxprotein ORF04410 8819 & 8820 transporter, LysE family ORF04411 8821 &8822 Lysophospholipase L2 (Lecithinase B) (PLDB) [3.1.1.5] ORF04412 8823& 8824 Cof protein ORF04413 8825 & 8826 HTH-type transcriptionalregulator metR (METR) ORF04414 8827 & 8828 Hypothetical membrane proteinyigM ORF04415 8829 & 88305-methyltetrahydropteroyltriglutamate--homocysteineS-methyltransferase(metE) [2.1.1.14] ORF04416 8831 & 8832 conserved hypothetical proteinORF04417 8833 & 8834 conserved hypothetical protein ORF04418 8835 & 88366-phospho-3-hexuloisomerase (PHI) ORF04419 8837 & 8838 PTS system,glucose-specific IIBC component (ptsG) [2.7.1.69] ORF04420 8839 & 8840transketolase (tkt) [2.2.1.1] ORF04421 8841 & 8842 transcriptionalregulator, rpiR family domain protein ORF04422 8843 & 8844transcriptional regulator, LysR family ORF04423 8845 & 8846 cytosinetransporter, putative ORF04424 8847 & 8848 amidase family protein,putative ORF04425 8849 & 8850 Carbamate kinase-like protein yahI[2.7.2.2] ORF04426 8851 & 8852 YahG-YlbE-like protein ORF04427 8853 &8854 YahF-FdrA-like protein ORF04428 8855 & 8856 conserved hypotheticalprotein ORF04429 8857 & 8858 isochorismatase family protein ORF044308859 & 8860 hypothetical protein ORF04431 8861 & 8862 Putativecarboxymethylenebutenolidase ORF04432 8863 & 8864 conserved hypotheticalprotein ORF04433 8865 & 8866 uridine phosphorylase (udp) [2.4.2.3]ORF04434 8867 & 8868 tricarboxylate transport protein TctA, putativeORF04435 8869 & 8870 conserved hypothetical protein ORF04436 8871 & 8872Bordetella uptake gene (bug) product superfamily ORF04437 8873 & 88744-hydroxy-2-oxoglutarate aldolase-2-dehydro-3-deoxyphosphogluconateORF04438 8875 & 8876 2-dehydro-3-deoxygalactonate kinase ORF04439 8877 &8878 transcriptional regulator, lclR family ORF04440 8879 & 8880 DNArecombination protein rmuC ORF04441 8881 & 8882 Ubiquinone-menaquinonebiosynthesis methyltransferase ubiE(EC 2.1.1.—) (UBIE) [2.1.1.—]ORF04442 8883 & 8884 unnamed protein product ORF04443 8885 & 88862-polyprenylphenol 6-hydroxylase (ubiB) [1.14.13.—] ORF04444 8887 & 8888o261 (YIGT) ORF04445 8889 & 8890 o261 ORF04446 8891 & 8892Sec-independent protein translocase TatC (tatC) ORF04447 8893 & 8894tatD gene product (YIGW) [3.1.21.—] ORF04448 8895 & 8896 transcriptionalactivator RfaH (rfaH) ORF04449 8897 & 88983-octaprenyl-4-hydroxybenzoate carboxy-lyase(Polyprenylp-hydroxybenzoate decarboxylase) (YIGC) [4.1.1.—] ORF044508899 & 8900 NAD(P)H-flavin reductase ORF04451 8901 & 8902 conservedhypothetical protein ORF04452 8903 & 8904 acetyl-CoA C-acyltransferaseFadA (fadA) [2.3.1.16] ORF04453 8905 & 8906 fatty oxidation complexalpha subunit FadB (fadB) [4.2.1.17 5.3.3.8 1.1.1.35 5.1.2.3] ORF044548907 & 8908 unnamed protein product; pepQ product, proline dipeptidase(PEPQ) [3.4.13.9] ORF04455 8909 & 8910 conserved hypothetical proteinTIGR00257 ORF04456 8911 & 8912 bis(5′-nucleosyl)-tetraphosphatase,symmetrical-Trk system potassium uptake protein TrkG, fusion ORF044578913 & 8914 protoporphyrin oxidase ORF04458 8915 & 8916 hypotheticalprotein ORF04459 8917 & 8918 molybdopterin-guanine dinucleotidebiosynthesis protein B (mobB) ORF04460 8919 & 8920 Molybdopterin-guaninedinucleotide biosynthesis protein A ORF04461 8921 & 8922 Protein yihDORF04462 8923 & 8924 YihE ORF04463 8925 & 8926 Thiol: disulfideinterchange protein dsbA precursor (por) [5.3.4.1] ORF04464 8927 & 8928Bacterial protein of unknown function (DUF945) superfamily ORF04465 8929& 8930 Acyltransferase domain protein ORF04466 8931 & 8932 DNApolymerase I (POL I) (polA) [2.7.7.7] ORF04467 8933 & 8934 essentialGTPase for cell cycle ORF04468 8935 & 8936 neutrophil protein ORF044698937 & 8938 hypothetical protein ORF04470 8939 & 8940 oxygen-independentcoproporphyrinogen III oxidase (hemN) ORF04471 8941 & 8942 hypotheticalprotein ORF04472 8943 & 8944 nitrogen regulation protein NR(I) (ntrC)ORF04473 8945 & 8946 Nitrogen regulation protein NR(II) [2.7.3.—]ORF04474 8947 & 8948 glutamine synthetase, type I (glnA) [6.3.1.2]ORF04475 8949 & 8950 GTP-binding protein TypA (typA) ORF04476 8951 &8952 hypothetical protein ORF04477 8953 & 8954 Glycerol-3-phosphateregulon repressor ORF04478 8955 & 8956 Hypothetical sugar kinase yihVORF04479 8957 & 8958 3-hydroxyisobutyrate dehydrogenase ORF04480 8959 &8960 Tagatose 1 ,6-diphosphate aldolase 2 (Tagatose-bisphosphatealdolase 2) (D- tagatose-1,6-bisphosphate aldolase 2) [4.1.—.—] ORF044818961 & 8962 2,3-butanediol dehydrogenase, putative ORF04482 8963 & 8964major facilitator family transporter, truncation, putative ORF04483 8965& 8966 major facilitator family transporter ORF04484 8967 & 8968 Aldose1-epimerase superfamily ORF04485 8969 & 8970 haloacid dehalogenase-likehydrolase, putative ORF04486 8971 & 8972 ribonuclease BN, putative[3.1.—.—] ORF04487 8973 & 8974 D-tyrosyl-tRNA(Tyr) deacylase (dtd)[3.1.—.—] ORF04488 8975 & 8976 conserved membrane protein [2.3.1.18]ORF04489 8977 & 8978 lipase, GDXG family VCA0490 ORF04490 8979 & 8980transcriptional regulator, Cro-Cl family ORF04491 8981 & 8982Ribbon-helix-helix protein, copG family domain protein ORF04492 8983 &8984 formate dehydrogenase accessory protein FdhE (fdhE) ORF04493 8985 &8986 formate dehydrogenase, gamma subunit [1.2.1.2] ORF04494 8987 & 8988formate dehydrogenase, beta subunit (FdxH) [1.2.1.2] ORF04495 8989 &8990 formate dehydrogenase, alpha subunit [1.2.1.2] ORF04496 8991 & 8992formate dehydrogenase, alpha subunit, selenocysteine-containing[1.2.1.2] ORF04497 8993 & 8994 formate dehydrogenase family accessoryprotein FdhD (fdhD) ORF04498 8995 & 8996 conserved hypothetical proteinORF04499 8997 & 8998 conserved hypothetical protein ORF04500 8999 & 9000Putative glycoporin ORF04501 9001 & 9002 B. subtilis YulD proteinhomolog lin2978 , putative ORF04502 9003 & 9004 Rhamnulose-1-phosphatealdolase ORF04503 9005 & 9006 L-rhamnose isomerase (rhaA) [5.3.1.14]ORF04504 9007 & 9008 sugar kinase [2.7.1.5] ORF04505 9009 & 9010hypothetical protein ORF04506 9011 & 9012 L-rhamnose operon Regulatoryprotein rhaS ORF04507 9013 & 9014 positive regulator for rhaRS operonORF04508 9015 & 9016 L-rhamnose-proton symport ORF04509 9017 & 9018Superoxide dismutase [Mn] (sodA) [1.15.1.1] ORF04510 9019 & 9020conserved hypothetical protein ORF04511 9021 & 90222-keto-3-deoxygluconate transporter (kdgT) ORF04512 9023 & 9024 MOSCdomain protein ORF04513 9025 & 9026 Sensor protein cpxA [2.7.3.—]ORF04514 9027 & 9028 Transcriptional Regulatory protein cpxR ORF045159029 & 9030 Periplasmic protein cpxP precursor ORF04516 9031 & 9032protein p34 ORF04517 9033 & 9034 6-phosphofructokinase I ORF04518 9035 &9036 Sulfate-binding protein precursor ORF04519 9037 & 9038CDP-diacylglycerol pyrophosphatase ORF04520 9039 & 9040 triosephosphateisomerase (tpiA) [5.3.1.1] ORF04521 9041 & 9042 Protein of unknownfunction (DUF1454) superfamily ORF04522 9043 & 9044 conserved membraneprotein ORF04523 9045 & 9046 conserved hypothetical protein superfamilyORF04524 9047 & 9048 Universal stress protein D (UspA) ORF04525 9049 &9050 Ferredoxin--NADP reductase (DA1) [1.18.1.2] ORF04526 9051 & 9052fructose-1,6-bisphosphatase, class II (glpX) [3.1.3.11] ORF04527 9053 &9054 glycerol kinase (glpK) [2.7.1.30] ORF04528 9055 & 9056 Glyceroluptake facilitator protein (Aquaglyceroporin) (glpF) ORF04529 9057 &9058 Protein of unknown function (DUF904) superfamily ORF04530 9059 &9060 regulator of ribonuclease activity A (rraA) ORF04531 9061 & 90621,4-dihydroxy-2-naphthoate octaprenyltransferase (menA) [2.5.—.—]ORF04532 9063 & 9064 heat shock protein HslVU, ATPase subunit HslU(hslU) ORF04533 9065 & 9066 peptidase component of the HslUV proteaseORF04534 9067 & 9068 cell division protein FtsN (ftsN) ORF04535 9069 &9070 regulator for deo operon, udp, cdd, tsx ORF04536 9071 & 9072Primosomal protein N′ ORF04537 9073 & 9074 ribosomal protein L31 (rpmE)ORF04538 9075 & 9076 Protein of unknown function (DUF1105) superfamilyORF04539 9077 & 9078 Met repressor ORF04540 9079 & 9080O-succinylhomoserine (thiol)-lyase (metB) [2.5.1.48] ORF04541 9081 &9082 AKII-HDII protein [2.7.2.4] ORF04542 9083 & 9084Nucleoside-specific channel-forming protein tsx precursor ORF04543 9085& 9086 5-nucleotidase family protein, putative ORF04544 9087 & 90885′-nucleotidase, C-terminal domain protein ORF04545 9089 & 90905-nucleotidase, putative ORF04546 9091 & 9092 conserved hypotheticalprotein ORF04547 9093 & 9094 5-nucleotidase family protein, putativeORF04548 9095 & 9096 5,10-methylenetetrahydrofolate reductase (metF)[1.7.99.5] ORF04549 9097 & 9098 catalase-peroxidase HPI (katG)[1.11.1.6] ORF04550 9099 & 9100 Hypothetical transport protein yijEORF04551 9101 & 9102 Protein of unknown function (DUF1287) superfamilyORF04552 9103 & 9104 conserved hypothetical protein ORF04553 9105 & 9106Glycerol dehydrogenase (NAD) [1.1.1.6] ORF04554 9107 & 9108transaldolase, putative ORF04555 9109 & 9110 Phosphoenolpyruvate-proteinphosphotransferase ptsA [2.7.3.9] ORF04556 9111 & 9112 PTS system,fructose-specific IIABC components ORF04557 9113 & 9114 PTS system,fructose-like-2 IIB component 1 (Phosphotransferase enzymeII, Bcomponent) [2.7.1. 69] ORF04558 9115 & 9116 pyruvate formate-lyase[2.3.1.54] ORF04559 9117 & 9118 similar to E. coli pyruvateformate-lyase activating enzyme ORF04560 9119 & 9120 AraC-typeDNA-binding domain-containing protein ORF04561 9121 & 9122 PTS systemfructose-like IIB component 2 (phosphotransfer) [2.7.1.69] ORF04562 9123& 9124 integral membrane protein, putative ORF04563 9125 & 9126phosphoenolpyruvate carboxylase (ppc) [4.1.1.31] ORF04564 9127 & 9128acetylornithine deacetylase (ArgE) (argE) [3.5.1.16] ORF04565 9129 &9130 N-acetyl-gamma-glutamyl-phosphate reductase (argC) [1.2.1.38]ORF04566 9131 & 9132 acetylglutamate kinase (argB) [2.7.2.8] ORF045679133 & 9134 argininosuccinate lyase (argH) [4.3.2.1] ORF04568 9135 &9136 Starvation sensing protein rspA ORF04569 9137 & 9138 majorfacilitator family transporter ORF04570 9139 & 9140 soluble pyridinenucleotide transhydrogenase (STH) [1.6.1.1] ORF04571 9141 & 9142Hydrogen peroxide-inducible genes activator (oxyR) ORF04572 9143 & 9144hippurate hydolase ORF04573 9145 & 9146 major facilitator familytransporter ORF04574 9147 & 9148 transcriptional regulator, TetR familyORF04575 9149 & 9150 ATPase of the AAA+ class ORF04576 9151 & 9152tRNA(uracil-5-)-methyltransferase (trmA) [2.1.1.35] ORF04577 9153 & 9154TonB-dependent vitamin B12 receptor (btuB) ORF04578 9155 & 9156glutamate racemase (murI) [5.1.1.3] ORF04579 9157 & 9158 hypotheticalprotein ORF04580 9159 & 9160 UDP-N-acetylenolpyruvoylglucosaminereductase (murB) [1.1.1.158] ORF04581 9161 & 9162 BirA bifunctionalprotein (BIRA) [6.3.4.15] ORF04582 9163 & 9164 pantothenate kinase(coaA) [2.7.1.33] ORF04583 9165 & 9166 translation elongation factorEF-Tu.B ORF04584 9167 & 9168 translation elongation factor EF-Tu.BORF04585 9169 & 9170 protein chain elongation factor EF-Tu ORF04586 9171& 9172 preprotein translocase SecE subunit (SecE) ORF04587 9173 & 9174transcription termination-antitermination factor NusG (nusG) ORF045889175 & 9176 ribosomal protein L11 (rplK) ORF04589 9177 & 9178 ribosomalprotein L1 (rplA) ORF04590 9179 & 9180 50S ribosomal subunit protein L10(RPLJ) ORF04591 9181 & 9182 ribosomal protein L7-L12 (rpIL) ORF045929183 & 9184 DNA-directed RNA polymerase, beta subunit (rpoB) [2.7.7.6]ORF04593 9185 & 9186 DNA-directed RNA polymerase, beta′ subunit (rpoC)[2.7.7.6] ORF04594 9187 & 9188 thiazole biosynthesis protein ThiH (thiH)ORF04595 9189 & 9190 4-methyl-5-(beta-hydroxyethyl)thiazolemonophosphate synthesis protein ThiG ORF04596 9191 & 9192 thiaminebiosynthesis protein ThiS (thiS) ORF04597 9193 & 9194 thiazolebiosynthesis adenylyltransferase ThiF (thiF) ORF04598 9195 & 9196thiamine-phosphate pyrophosphorylase (thiE) [2.5.1.3] ORF04599 9197 &9198 Thiamine biosynthesis protein thiC ORF04600 9199 & 9200 Regulatorof sigma D ORF04601 9201 & 9202 MutT-nudix family protein [3.6.1.—]ORF04602 9203 & 9204 uroporphyrinogen decarboxylase (hemE) [4.1.1.37]ORF04603 9205 & 9206 endonuclease V (deoxyinosine 3endoduclease)ORF04604 9207 & 9208 Protein of unknown function, DUF superfamilyORF04605 9209 & 9210 DNA-binding protein HU-alpha (NS2) (HU-2) (HU)ORF04606 9211 & 9212 unnamed protein product ORF04607 9213 & 9214 Zincresistance-associated protein precursor ORF04608 9215 & 9216 sensorkinase for HydG, hydrogenase 3 activity [2.7.3.—] ORF04609 9217 & 9218Transcriptional Regulatory protein zraR (sigma54) ORF04610 9219 & 9220phosphoribosylamine--glycine ligase (purD) [6.3.4.13] ORF04611 9221 &9222 bifunctional purine biosynthesis protein PurH (purH) ORF04612 9223& 9224 conserved hypothetical protein ORF04613 9225 & 9226 hypotheticalprotein ORF04614 9227 & 9228 conserved hypothetical protein ORF046159229 & 9230 acetyltransferase, GNAT family (putative) [2.3.1.—] ORF046169231 & 9232 homoserine O-succinyltransferase (metA) [2.3.1.46] ORF046179233 & 9234 malate synthase A (aceB) [2.3.3.9] ORF04618 9235 & 9236isocitrate lyase (aceA) [4.1.3.1] ORF04619 9237 & 9238 Isocitratedehydrogenase kinase-phosphatase(EC 3.1.3.—) (IDH kinase- phosphatase)(IDHK-P) [2.7.1. 116] ORF04620 9239 & 9240 transcriptional regulator,putative ORF04621 9241 & 9242 hypothetical protein ORF04622 9243 & 92445-methyltetrahydrofolate--homocysteine methyltransferase (metH)[2.1.1.13] ORF04623 9245 & 9246 conserved hypothetical protein ORF046249247 & 9248 Na-Pi-cotransporter II-related protein, putative ORF046259249 & 9250 Peptidase E (Alpha-aspartyl dipeptidase)(Asp-specificdipeptidase) (Dipeptidase E) (alpha) [3.4.13.21] ORF046269251 & 9252 L-sorbose 1-phosphate reductase [1.1.1.—] ORF04627 9253 &9254 PTS system, mannose-specific IID component ORF04628 9255 & 9256 PTSsystem, mannose-specific IIC component [2.7.1.69] ORF04629 9257 & 9258sorbose-permease PTS system IIB component (EIII-MAN) [2.7.1.69] ORF046309259 & 9260 PTS system, sorbose-specific IIA component (EIIA-Sor)(Sorbose-permease IIA component) (Phosphotransferase enzyme II, Acomponent)(EC 2.7.1.69) (EIII-F- Sor) [2.7.1.69] ORF04631 9261 & 9262D-glucitol-6-phosphate dehydrogenase [1.1.1.140] ORF04632 9263 & 9264Putative transcriptional regulator of sorbose uptake and ORF04633 9265 &9266 RNA pseudouridylate synthase family protein [4.2.1.70] ORF046349267 & 9268 conserved hypothetical protein ORF04635 9269 & 9270 asparatekinase, monofunctional class [2.7.2.4] ORF04636 9271 & 9272glucose-6-phosphate isomerase (pgi) [5.3.1.9] ORF04637 9273 & 9274conserved hypothetical protein ORF04638 9275 & 9276 lipoprotein,putative ORF04639 9277 & 9278 Protein of unknown function (DUF1017)superfamily ORF04640 9279 & 9280 otnG protein (o698) ORF04641 9281 &9282 conserved hypothetical protein ORF04642 9283 & 9284 PsiE proteinhomolog ORF04643 9285 & 9286 Maltose transport system permease proteinmalG (malG) ORF04644 9287 & 9288 Maltose transport system permeaseprotein malF (membrane) ORF04645 9289 & 9290 Maltose-binding periplasmicprotein precursor ORF04646 9291 & 9292 Maltose-maltodextrin transportATP-binding protein malK ORF04647 9293 & 9294 Maltoporin precursor(Maltose-inducible porin) (malL) ORF04648 9295 & 9296 Maltose operonperiplasmic protein precursor (MalM) superfamily ORF04649 9297 & 9298conserved hypothetical protein ORF04650 9299 & 9300 chorismate lyase[4.—.—.—] ORF04651 9301 & 9302 4-hydroxybenzoate polyprenyl transferase(ubiA) [2.5.1.—] ORF04652 9303 & 9304 glycerol-3-phosphateO-acyltransferase [2.3.1.15] ORF04653 9305 & 9306 diacylglycerol kinase(DGK) [2.7.1.107] ORF04654 9307 & 9308 LexA repressor (lexA) [3.4.21.88]ORF04655 9309 & 9310 DNA-damage-inducible protein F, truncation ORF046569311 & 9312 Protein yjbJ-related protein ORF04657 9313 & 9314 Zincuptake regulation protein ORF04658 9315 & 9316 tRNA-dihydrouridinesynthase A [1.—.—.—] ORF04659 9317 & 9318 conserved hypothetical proteinORF04660 9319 & 9320 quinone oxidoreductase ORF04661 9321 & 9322Putative oxidoreductase ORF04662 9323 & 9324 metabolite-proton symporterORF04663 9325 & 9326 enoyl-CoA hydratase-isomerase family proteinORF04664 9327 & 9328 acetyl-CoA-transferase subunit, putative [2.8.3.—]ORF04665 9329 & 9330 LACI-FAMILY TRANSCRIPTION REGULATOR ORF04666 9331 &9332 replicative DNA helicase (dnaB) [3.6.1.—] ORF04667 9333 & 9334alanine racemase (alr) [5.1.1.1] ORF04668 9335 & 9336 transporter,putative ORF04669 9337 & 9338 NadR homolog ORF04670 9339 & 9340Aromatic-amino-acid aminotransferase ORF04671 9341 & 9342 2-oxoglutaratedehydrogenase, E1 component (sucA) [1.2.4.2] ORF04672 9343 & 93442-oxoglutarate dehydrogenase, E2 component, dihydrolipoamidesuccinyltransferase (sucB) [2.3.1.61] ORF04673 9345 & 9346dihydrolipoamide dehydrogenase (IpdA) [1.8.1.4] ORF04674 9347 & 9348Succinyl-CoAsynthetase beta chain (AF326913) [6.2.1.5] ORF04675 9349 &9350 Succinyl-CoA synthetase alpha chain [6.2.1.5] ORF04676 9351 & 9352anion transporter family protein ORF04677 9353 & 9354 two-componentresponse regulator ORF04678 9355 & 9356 Putative lactate dehydrogenaseORF04679 9357 & 9358 Putative transport sensor protein ORF04680 9359 &9360 HAD superfamily (subfamily IIIB) phosphatase, TIGR01672 (AphA)[3.1.3.—] ORF04681 9361 & 9362 conserved hypothetical protein TIGR00149ORF04682 9363 & 9364 Protein yjbR ORF04683 9365 & 9366 excinuclease ABC,A subunit (uvrA) ORF04684 9367 & 9368 ssDNA-binding protein ORF046859369 & 9370 conserved hypothetical protein ORF04686 9371 & 9372 EALdomain protein ORF04687 9373 & 9374 regulatory protein soxS ORF046889375 & 9376 redox-sensitive transcriptional activator SoxR (soxR)ORF04689 9377 & 9378 xanthine-uracil permease family protein ORF046909379 & 9380 Na+—H+ antiporter ORF04691 9381 & 9382 transcriptionalregulator, LysR family, putative ORF04692 9383 & 9384 conservedhypothetical protein ORF04693 9385 & 9386 murein hydrolase exporterORF04694 9387 & 9388 LrgB-like family superfamily ORF04695 9389 & 9390Sodium, solute symporter family protein (PutP) ORF04696 9391 & 9392Protein of unknown function, DUF485 superfamily ORF04697 9393 & 9394acetate--CoA ligase (acsA) [6.2.1.1] ORF04698 9395 & 9396 nitritereductase (cytochrome; ammonia-forming) (nrfA) [1.7.2.2] ORF04699 9397 &9398 formate-dependent nitrite reductase NrfB ORF04700 9399 & 9400 NrfCprotein (NrfC) [1.—.—.—] ORF04701 9401 & 9402 NrfD protein (NrfD)[1.—.—.—] ORF04702 9403 & 9404 cytochrome c-type biogenesis protein CcmF(ccmF) ORF04703 9405 & 9406 formate-dependent nitrite reductase ORF047049407 & 9408 Formate-dependent nitrite reductase complex nrfG subunit(nrfF) ORF04705 9409 & 9410 conserved hypothetical protein ORF04706 9411& 9412 glutamate-aspartate symport protein (gltP) ORF04707 9413 & 9414conserved hypothetical protein ORF04708 9415 & 9416 ABC transporter,ATP-binding protein ORF04709 9417 & 9418 ABC transporter, nucleotidebinding-ATPase protein [dipeptide] ORF04710 9419 & 9420 ABC transporter,permease protein (AE006058) ORF04711 9421 & 9422 ABC transporterpermease protein (AE005357) ORF04712 9423 & 9424 ABC transporter,periplasmic oligo-dipeptide-nickel binding protein (AE005357) ORF047139425 & 9426 formate dehydrogenase, alpha subunit [1.2.1.2] ORF04714 9427& 9428 formate dehydrogenase, alpha subunit, putative [1.2.1.2] ORF047159429 & 9430 matches PS00443: Glutamine amidotransferases class-II activesite; similar to Pseudomonas cepacia fusaric acid resistance proteinFusA ORF04716 9431 & 9432 Fusaric acid resistance protein conservedregion family ORF04717 9433 & 9434 HlyD family secretion protein,putative ORF04718 9435 & 9436 conserved hypothetical protein ORF047199437 & 9438 metallo-beta-lactamase superfamily protein ORF04720 9439 &9440 glucokinase (EC 2.7.1.2) , putative [2.7.1.21 ORF04721 9441 & 9442ribulose-phosphate 3-epimerase (rpe) [5.1.3.1] ORF04722 9443 & 9444ribose ABC transporter, permease protein (permease) ORF04723 9445 & 9446ribose ABC transporter, permease protein (ECOWU89) ORF04724 9447 & 9448D-allose transport ATP-binding protein alsA (aldose) ORF04725 9449 &9450 hypothetical protein ORF04726 9451 & 9452 D-allose-bindingperiplasmic protein precursor (ALBP) ORF04727 9453 & 9454Transcriptional regulator, putative ORF04728 9455 & 9456 hypotheticalprotein ORF04729 9457 & 9458 ribose 5-phosphate isomerase B (rpiB)[5.3.1.6] ORF04730 9459 & 9460 conserved hypothetical protein ORF047319461 & 9462 PhnP protein (phnp) ORF04732 9463 & 9464 PhnO proteinORF04733 9465 & 9466 phosphonate metabolism protein-1,5-bisphosphokinase(PRPP-forming) PhnN (phnN) ORF04734 9467 & 9468 phosphonate metabolismprotein PhnM (phnM) ORF04735 9469 & 9470 phosphonate C-P lyase systemprotein PhnL (phnL) ORF04736 9471 & 9472 phosphonate C-P lyase systemprotein PhnK (phnK) ORF04737 9473 & 9474 Phosphonate metabolism proteinPhnJ ORF04738 9475 & 9476 Bacterial phosphonate metabolism protein(PhnI) ORF04739 9477 & 9478 Bacterial phosphonate metabolism protein(PhnH) ORF04740 9479 & 9480 PhnG protein ORF04741 9481 & 9482phosphonates metabolism transcriptional regulator PhnF (phnF) ORF047429483 & 9484 membrane channel protein component of Pn transporterORF04743 9485 & 9486 phosphonate ABC transporter, substrate-bindingprotein, putative ORF04744 9487 & 9488 phosphonate ABC transporter,ATP-binding protein (phnC) ORF04745 9489 & 9490 PhnB protein ORF047469491 & 9492 alkylphosphonate utilization operon protein PhnA ORF047479493 & 9494 conserved hypothetical protein ORF04748 9495 & 9496conserved hypothetical protein ORF04749 9497 & 9498 amino acid MFStransporter (PPII) ORF04750 9499 & 9500 Sensor protein basS-pmrBORF04751 9501 & 9502 Transcriptional Regulatory protein basR-pmrAORF04752 9503 & 9504 predicted membrane-associated, metal-dependenthydrolase ORF04753 9505 & 9506 Arginine-agmatine antiporter ORF047549507 & 9508 HTH-type transcriptional regulator adiY ORF04755 9509 & 9510arginine decarboxylase [4.1.1.19] ORF04756 9511 & 9512 argininedecarboxylase adi, biodegradative (ldc) [4.1.1.19] ORF04757 9513 & 9514Melibiose operon regulatory protein (AF049243) ORF04758 9515 & 9516glycosyl hydrolase, family 4, putative [3.2.1.22] ORF04759 9517 & 9518membrane protein, putative ORF04760 9519 & 9520 fumarate hydratase classI, anaerobic [4.2.1.2] ORF04761 9521 & 9522 anaerobic C4-dicarboxylatemembrane transporter ORF04762 9523 & 9524 conserved hypothetical proteinORF04763 9525 & 9526 response regulator VC1604 (partial) ORF04764 9527 &9528 Sensor protein dcuS [2.7.3.—] ORF04765 9529 & 9530 hypotheticalprotein ORF04766 9531 & 9532 YjdI-like protein ORF04767 9533 & 9534acetyltransferase, GNAT family (GNAT) ORF04768 9535 & 9536 lysyl-tRNAsynthetase (lysS) [6.1.1.6] ORF04769 9537 & 9538 PTR2-family transportprotein STY0750 (POT) ORF04770 9539 & 9540 CadA (ldc) [4.1.1.18]ORF04771 9541 & 9542 transport of lysine-cadaverine ORF04772 9543 & 9544transcriptional activator ORF04773 9545 & 9546 hypothetical proteinORF04774 9547 & 9548 transcriptional regulator, TetR family domainprotein, putative ORF04775 9549 & 9550 Thiohdisulfide interchangeprotein dsbD precursor (dsbD) [1.8.1.8] ORF04776 9551 & 9552 divalentcation tolerance protein cytochrome c biogenesis ORF04777 9553 & 9554Anaerobic C4-dicarboxylate transporter dcuA (DcuA) ORF04778 9555 & 9556aspartate ammonia-lyase (aspA) [4.3.1.1] ORF04779 9557 & 9558 suppressorof F exclusion of bacteriophage T7 ORF04780 9559 & 9560 Amino acidpermease superfamily ORF04781 9561 & 9562 chaperonin, 10 kDa (groES)ORF04782 9563 & 9564 chaperonin GroEL (groL) ORF04783 9565 & 9566conserved hypothetical protein ORF04784 9567 & 9568 conservedhypothetical protein ORF04785 9569 & 9570 lysine 2; 3-aminomutaseORF04786 9571 & 9572 translation elongation factor P (efp) ORF04787 9573& 9574 Entericidin B precursor-related protein ORF04788 9575 & 9576Entericidin B precursor-related protein ORF04789 9577 & 9578 SugELORF04790 9579 & 9580 Outer membrane lipoprotein blc precursor ORF047919581 & 9582 beta-lactamase ORF04792 9583 & 9584 fumarate reductase,anaerobic, membrane anchor polypeptide ORF04793 9585 & 9586 Fumaratereductase subunit C (Fumarate reductase 15 kDa hydrophobicprotein)(frdC) [1.3.99.1] ORF04794 9587 & 9588 fumarate reductase, anaerobic,iron-sulfur protein subunit ORF04795 9589 & 9590 fumarate reductase,flavoprotein subunit (frdA) [1.3.99.1] ORF04796 9591 & 9592 lysyl-tRNAsynthetase-related protein GenX ORF04797 9593 & 9594 amino acid permeaseORF04798 9595 & 9596 conserved hypothetical protein ORF04799 9597 & 9598conserved hypothetical protein ORF04800 9599 & 9600 unnamed proteinproduct ORF04801 9601 & 9602 phosphatidylserine decarboxylasephospholipid synthesis ORF04802 9603 & 9604 Predicted GTPases ORF048039605 & 9606 Oligoribonuclease ORF04804 9607 & 9608 unnamed proteinproduct ORF04805 9609 & 9610 predicted sugar kinase ORF04806 9611 & 9612conserved hypothetical protein TIGR00150 ORF04807 9613 & 9614N-acetylmuramoyl-I-alanine amidase II a murein hydrolase ORF04808 9615 &9616 DNA mismatch repair protein mutL ORF04809 9617 & 9618 tRNAdelta(2)-isopentenylpyrophosphate transferase (miaA) [2.5.1.8] ORF048109619 & 9620 host factor I (HF-I) ORF04811 9621 & 9622 GTP-bindingprotein hflX ORF04812 9623 & 9624 HflK protein (hflK) [3.4.—.—] ORF048139625 & 9626 HflC protein (hflC) ORF04814 9627 & 9628 hypotheticalprotein ORF04815 9629 & 9630 conserved hypothetical protein ORF048169631 & 9632 hypothetical protein ORF04817 9633 & 9634 adenylosuccinatesynthetase (purA) [6.3.4.4] ORF04818 9635 & 9636 Predictedtranscriptional regulator ORF04819 9637 & 9638 ribonuclease R (rnr)[3.1.—.—] ORF04820 9639 & 9640 RNA methyltransferase, TrmH family, group3 ORF04821 9641 & 9642 conserved hypothetical protein ORF04822 9643 &9644 PspA-IM30 family protein ORF04823 9645 & 9646 conservedhypothetical protein ORF04824 9647 & 9648 predicted membrane protein(o132) ORF04825 9649 & 9650 Protein of unknown function (DUF1190)superfamily ORF04826 9651 & 9652 glutathionylspermidine synthase (O386)ORF04827 9653 & 9654 fadE8 ORF04828 9655 & 9656 Protein of unknownfunction (DUF1471) superfamily ORF04829 9657 & 9658 Protein of unknownfunction (DUF1471) family ORF04830 9659 & 9660 Hydrolases of thealpha-beta superfamily (putative) ORF04831 9661 & 9662 transcriptionalregulator, DeoR family, putative ORF04832 9663 & 9664 PredictedZn-dependent hydrolase of the beta-lactamase fold ORF04833 9665 & 9666Putative sugar-specific permease, SgaT-UlaA superfamily, putativeORF04834 9667 & 9668 putative IIB protein of PTS system ORF04835 9669 &9670 protein-Npi-phosphohistidine-sugar phosphotransferase (Ntr-type)[2.7.1.69] ORF04836 9671 & 9672 hexulose-6-phosphate synthase (humps)ORF04837 9673 & 9674 hexulose-6-phosphate isomerase, putative ORF048389675 & 9676 AraD ORF04839 9677 & 9678 Protein of unknown function(DUF1471) superfamily ORF04840 9679 & 9680 30S ribosomal protein (rpS6)ORF04841 9681 & 9682 Single-strand binding protein family ORF04842 9683& 9684 ribosomal protein S18 (rpsR) ORF04843 9685 & 9686 ribosomalprotein L9 (rplI) ORF04844 9687 & 9688 conserved hypothetical proteinORF04845 9689 & 9690 MFS transporter, phthalate permease family,putative ORF04846 9691 & 9692 Putative oxidoreductase ORF04847 9693 &9694 acetyl-CoA-transferase subunit, putative ORF04848 9695 & 9696enoyl-CoA hydratase-isomerase FadB1x, putative [4.2.1.55] ORF04849 9697& 9698 3-dehydroshikimate dehydratase ORF04850 9699 & 9700 conservedhypothetical protein ORF04851 9701 & 97023-oxoacyl-(acyl-carrier-protein)reductase ORF04852 9703 & 9704Opacity-associated protein A superfamily ORF04853 9705 & 9706 Putativeconserved protein ORF04854 9707 & 9708 FKBP-type 22 kDa peptidyl-prolylcis-trans isomerase (rotamase) [5.2.1.8] ORF04855 9709 & 9710D-serine-D-alanine-glycine transporter ORF04856 9711 & 9712 ScdAprotein, putative ORF04857 9713 & 9714 integral membrane protein (f324)ORF04858 9715 & 9716 conserved hypothetical protein ORF04859 9717 & 9718Predicted transcriptional regulators ORF04860 9719 & 97202′,3′-cyclic-nucleotide 2′-phosphodiesterase (cpdB) [3.1.4.16] ORF048619721 & 9722 conserved hypothetical protein YtfJ-family, TIGR01626ORF04862 9723 & 9724 3′(2′),5′-bisphosphate nucleotidase (cysQ)[3.1.3.7] ORF04863 9725 & 9726 Protein of unknown function (DUF1107)superfamily ORF04864 9727 & 9728 Hypothetical UPF0053 protein ytfLORF04865 9729 & 9730 peptide methionine sulfoxide reductase ORF048669731 & 9732 unnamed protein product ORF04867 9733 & 9734 Family ofunknown function (DUF490) family ORF04868 9735 & 9736 HypotheticalUPF0131 protein ytfP ORF04869 9737 & 9738 inorganic pyrophosphatase(ppa) [3.6.1.1] ORF04870 9739 & 9740 periplasmic ribose-binding proteinORF04871 9741 & 9742 ATP binding protein of ABC transporter ORF048729743 & 9744 ribose ABC transporter, permease protein VCA0129 ORF048739745 & 9746 ribose ABC transporter, permease protein ORF04874 9747 &9748 fructose-1,6-bisphosphatase (fbp) [3.1.3.11] ORF04875 9749 & 9750conserved hypothetical protein ORF04876 9751 & 9752UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelateligase (mpl) ORF04877 9753 & 9754 Hypothetical UPF0307 protein yjgA (x96protein) ORF04878 9755 & 9756 PmbA protein (pmbA) ORF04879 9757 & 9758Soluble cytochrome b562 precursor (562) ORF04880 9759 & 9760 Anaerobicribonucleoside-triphosphate reductase activating protein(EC 1.97.1.4)(Class III anaerobic ribonucleotide reductase smallcomponent) [1.97.1.4]ORF04881 9761 & 9762 anaerobic ribonucleoside-triphosphate reductase[1.17.4.2] ORF04882 9763 & 9764 hypothetical protein ORF04883 9765 &9766 alpha,alpha-phosphotrehalase (treC) [3.2.1.93] ORF04884 9767 & 9768PTS system, trehalose-specific IIBC component (treP) [2.7.1.69] ORF048859769 & 9770 trehalose operon repressor (treR) ORF04886 9771 & 9772magnesium-translocating P-type ATPase (mgtA) [3.6.3.2] ORF04887 9773 &9774 endoribonuclease L-PSP, putative ORF04888 9775 & 9776 aspartatecarbamoyltransferase, regulatory subunit (pyrI) ORF04889 9777 & 9778aspartate carbamoyltransferase (pyrB) [2.1.3.2] ORF04890 9779 & 9780arginine repressor ORF04891 9781 & 9782 membrane protein, putativeORF04892 9783 & 9784 ornithine carbamoyltransferase (argF) [2.1.3.3]ORF04893 9785 & 9786 carbamate kinase (arcC) [2.7.2.2] ORF04894 9787 &9788 arginine deiminase (arcA) [3.5.3.6] ORF04895 9789 & 9790 ProteinyjgK ORF04896 9791 & 9792 ornithine carbamoyltransferase (argF)[2.1.3.3] ORF04897 9793 & 9794 Protein ORF04898 9795 & 9796 hypotheticalprotein ORF04899 9797 & 9798 acetyltransferase, GNAT family familyORF04900 9799 & 9800 probable innner membrane protein STY4813 ORF049019801 & 9802 valyl-tRNA synthetase (valS) [6.1.1.9] ORF04902 9803 & 9804DNA polymerase III, chi subunit [2.7.7.7] ORF04903 9805 & 9806 Cytosolaminopeptidase (Leucine aminopeptidase) (LAP)(Leucyl aminopeptidase)(Aminopeptidase A-I) (LAP) [3.4.11.1] ORF04904 9807 & 9808 conservedhypothetical protein ORF04905 9809 & 9810 unnamed protein productORF04906 9811 & 9812 hypothetical protein ORF04907 9813 & 9814 predictedpermease ORF04908 9815 & 9816 conserved ATP-binding protein ORF049099817 & 9818 L-idonate Regulatory protein (gnt) ORF04910 9819 & 9820Gnt-II system L-idonate transporter ORF04911 9821 & 9822 PROBABLEGLUCONATE 5-DEHYDROGENASE OXIDOREDUCTASE PROTEIN [1.1.1.69] ORF049129823 & 9824 2,3-butanediol dehydrogenase [1.1.1.264] ORF04913 9825 &9826 thermoresistant gluconokinase [2.7.1.12] ORF04914 9827 & 9828NADP-dependent alcohol dehydrogenase [1.1.1.—] ORF04915 9829 & 9830Integrase ORF04916 9831 & 9832 CII protein ORF04917 9833 & 9834 Betaprotein ORF04918 9835 & 9836 Polarity suppression protein (Ambermutation-suppressing protein) ORF04919 9837 & 9838 Transactivationprotein ORF04920 9839 & 9840 Glycoprotein 3 (Capsid size determinationprotein) ORF04921 9841 & 9842 phage DNA binding protein ORF04922 9843 &9844 unnamed protein product; cl gene product (AA 1-137) ORF04923 9845 &9846 conserved hypothetical protein ORF04924 9847 & 9848 conservedhypothetical protein ORF04925 9849 & 9850 Bacteriophage P4 DNA primase[2.7.7.—] ORF04926 9851 & 9852 hypothetical protein ORF04927 9853 & 9854hypothetical protein ORF04928 9855 & 9856 ATP-dependent protease LaORF04929 9857 & 9858 conserved hypothetical protein ORF04930 9859 & 9860conserved hypothetical protein ORF04931 9861 & 9862 unnamed proteinproduct; ORF616 ORF04932 9863 & 9864 Kelch motif domain protein ORF049339865 & 9866 Oligogalacturonate-specific porin protein (KdgM) superfamilyORF04934 9867 & 9868 FimB protein ORF04935 9869 & 9870 recombinaseinvolved in phase variation regulator for ORF04936 9871 & 9872 type 1fimbrial protein fimA precursor ORF04937 9873 & 9874 fimbrial proteinORF04938 9875 & 9876 Chaperone protein fimC precursor ORF04939 9877 &9878 Outer membrane usher protein fimD precursor ORF04940 9879 & 9880FimF protein precursor ORF04941 9881 & 9882 fimbrial morphology ORF049429883 & 9884 FimH ORF04943 9885 & 9886 mannonate dehydratase (uxuA)[4.2.1.8] ORF04944 9887 & 9888 D-mannonate oxidoreductase [1.1.1.57]ORF04945 9889 & 9890 regulator of uxu operon ORF04946 9891 & 9892Protein of unknown function (DUF1316) subfamily ORF04947 9893 & 9894phosphoenolpyruvate-protein phosphotransferase, EI-HPr-EIIA componentsORF04948 9895 & 9896 dihydroxyacetone kinase, L subunit [2.7.1.—]ORF04949 9897 & 9898 dihydroxyacetone kinase, DhaK subunit (dhaK)[2.7.1.—] ORF04950 9899 & 9900 hypothetical protein ORF04951 9901 & 9902glycerol dehydrogenase CgrD (NAD) [1.1.1.6] ORF04952 9903 & 9904putative transporter CgxT ORF04953 9905 & 9906 invasion protein IbeAORF04954 9907 & 9908 choline-glycine betaine transporter ORF04955 9909 &9910 Glycerate kinase [2.7.1. 31] ORF04956 9911 & 99122-hydroxy-3-oxopropionate reductase [1.1.1.60] ORF04957 9913 & 9914hydroxypyruvate isomerase [5.3.1.22] ORF04958 9915 & 9916 hypotheticalprotein ORF04959 9917 & 9918 glyoxylate carboligase [4.1.1.47] ORF049609919 & 9920 glyoxylate carboligase [4.1.1.47] ORF04961 9921 & 9922 NtrCfamily Transcriptional regulator, ATPase domain ORF04962 9923 & 9924invasion protein IbeA ORF04963 9925 & 9926 hypothetical protein ORF049649927 & 9928 Na+—H+ antiporter NhaC (nhaC) ORF04965 9929 & 9930Hypothetical transcriptional regulator yjiE ORF04966 9931 & 9932hypothetical protein ORF04967 9933 & 9934 isoaspartyl dipeptidase (iadA)[3.4.19.—] ORF04968 9935 & 9936 uncharacterized membrane proteinORF04969 9937 & 9938 Nucleoside recognition domain protein ORF04970 9939& 9940 RNA 2′-phosphotransferase, Tpt1 - KptA family domain proteinORF04971 9941 & 9942 conserved hypothetical protein ORF04972 9943 & 9944membrane protein, putative ORF04973 9945 & 9946 SrgB ORF04974 9947 &9948 conserved hypothetical protein ORF04975 9949 & 9950 Activator of2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) ORF049769951 & 9952 2-hydroxyglutaryl-CoA dehydratase subunit beta homologORF04977 9953 & 9954 Protein of unknown function (DUF445) superfamilyORF04978 9955 & 9956 transposase ORF04979 9957 & 9958 conserved proteinORF04980 9959 & 9960 conserved hypothetical protein ORF04981 9961 & 9962Type I restriction enzyme EcoKI specificity protein (S protein)(S.EcoKI) [3.1.21.3] ORF04982 9963 & 9964 Type I restriction enzyme EcoKI Mprotein (M. EcoKI) [2.1.1.72] ORF04983 9965 & 9966 Type I restrictionenzyme EcoKI R protein (R. EcoKI) [3.1.21.3] ORF04984 9967 & 9968 Mrrrestriction system protein (EcoKMrr) ORF04985 9969 & 9970 CobW-P47Kfamily protein domain protein ORF04986 9971 & 9972 Uncharacterized smallprotein ORF04987 9973 & 9974 YjiY ORF04988 9975 & 9976 Methyl-acceptingchemotaxis protein I (MCP-I) (Serine chemoreceptorprotein) (MCP-I)ORF04989 9977 & 9978 C4-dicarboxylate transporter large subunit (DctM)ORF04990 9979 & 9980 TRAP transporter, DctQ-like membrane proteinsuperfamily ORF04991 9981 & 9982 DctP protein ORF04992 9983 & 9984Na+—H+ antiporter ORF04993 9985 & 9986 yjjM gene product ORF04994 9987 &9988 sorbitol dehydrogenase, putative [1.1.1.14] ORF04995 9989 & 9990hypothetical protein

TABLE 2 142 preferred antigens ORF00010 ORF00017 ORF00029 ORF00041ORF00130 ORF00250 ORF00251 ORF00252 ORF00332 ORF00333 ORF00342 ORF00353ORF00394 ORF00405 ORF00469 ORF00631 ORF00773 ORF00905 ORF00908 ORF01034ORF01037 ORF01056 ORF01090 ORF01103 ORF01104 ORF01108 ORF01115 ORF01118ORF01119 ORF01194 ORF01202 ORF01207 ORF01208 ORF01214 ORF01228 ORF01236ORF01337 ORF01338 ORF01339 ORF01340 ORF01341 ORF01342 ORF01344 ORF01348ORF01349 ORF01350 ORF01351 ORF01365 ORF01371 ORF01462 ORF01477 ORF01491ORF01503 ORF01506 ORF01589 ORF01590 ORF01592 ORF01656 ORF01660 ORF01705ORF01722 ORF01723 ORF01855 ORF01932 ORF02307 ORF02314 ORF02392 ORF02412ORF02424 ORF02470 ORF02554 ORF02735 ORF02816 ORF02828 ORF02829 ORF02830ORF02831 ORF02832 ORF02833 ORF02834 ORF02847 ORF02866 ORF02868 ORF02878ORF02888 ORF03011 ORF03020 ORF03021 ORF03025 ORF03044 ORF03166 ORF03174ORF03176 ORF03235 ORF03252 ORF03262 ORF03300 ORF03348 ORF03349 ORF03364ORF03459 ORF03501 ORF03504 ORF03507 ORF03512 ORF03515 ORF03516 ORF03517ORF03518 ORF03519 ORF03520 ORF03522 ORF03535 ORF03539 ORF03540 ORF03541ORF03542 ORF03575 ORF03576 ORF03579 ORF03580 ORF03613 ORF04162 ORF04163ORF04184 ORF04203 ORF04204 ORF04282 ORF04390 ORF04391 ORF04402 ORF04477ORF04608 ORF04693 ORF04694 ORF04844 ORF04845 ORF04849 ORF04851 ORF04922ORF04924 ORF04925 Cytoplasmic location: ORF00252, ORF01056, ORF01337,ORF01341, ORF01344, ORF01348, ORF01371, ORF01477, ORF02424, ORF02833,ORF03348, ORF03504, ORF03542, ORF04162, ORF04844. Inner membranelocation: ORF00010, ORF00017, ORF00029, ORF00041, ORF00130, ORF00332,ORF00333, ORF00342, ORF00353, ORF00394, ORF00405, ORF00631, ORF00773,ORF00905, ORF01034, ORF01037, ORF01090, ORF01103, ORF01104, ORF01108,ORF01115, ORF01118, ORF01119, ORF01194, ORF01202, ORF01207, ORF01208,ORF01214, ORF01338, ORF01339, ORF01349, ORF01350, ORF01365, ORF01462,ORF01491, ORF01503, ORF01506, ORF01590, ORF01705, ORF01722, ORF01723,ORF01855, ORF01932, ORF02314, ORF02392, ORF02412, ORF02470, ORF02554,ORF02735, ORF02847, ORF02868, ORF02878, ORF02888, ORF03020, ORF03021,ORF03025, ORF03044, ORF03174, ORF03176, ORF03235, ORF03300, ORF03349,ORF03364, ORF03459, ORF03501, ORF03507, ORF03512, ORF03519, ORF03520,ORF03535, ORF03539, ORF03540, ORF03575, ORF03576, ORF03579, ORF03580,ORF04163, ORF04184, ORF04203, ORF04204, ORF04402, ORF04477, ORF04608,ORF04693, ORF04694, ORF04845, ORF04849, ORF04851, ORF04922, ORF04924,ORF04925. Outer membrane location: ORF00250, ORF00251, ORF00469,ORF01228, ORF01236, ORF01340, ORF01342, ORF01351, ORF01592, ORF01656,ORF02829, ORF02831, ORF02834, ORF03166, ORF03517, ORF03613, ORF04282.Periplasmic location: ORF00908, ORF01589, ORF01660, ORF02307, ORF02816,ORF02828, ORF02830, ORF02832, ORF02866, ORF03011, ORF03252, ORF03262,ORF03515, ORF03516, ORF03518, ORF03522, ORF03541, ORF04390, ORF04391

TABLE 3 low homology with commensal strains ORF00010, ORF00016,ORF00017, ORF00018, ORF00019, ORF00020, ORF00025, ORF00028, ORF00029,ORF00030, ORF00031, ORF00032, ORF00034, ORF00035, ORF00036, ORF00037,ORF00041, ORF00042, ORF00043, ORF00044, ORF00045, ORF00046, ORF00047,ORF00048, ORF00049, ORF00050, ORF00051, ORF00052, ORF00053, ORF00054,ORF00055, ORF00056, ORF00057, ORF00058, ORF00059, ORF00060, ORF00061,ORF00062, ORF00064, ORF00065, ORF00066, ORF00067, ORF00068, ORF00069,ORF00070, ORF00089, ORF00090, ORF00106, ORF00107, ORF00119, ORF00120,ORF00121, ORF00130, ORF00134, ORF00150, ORF00151, ORF00152, ORF00163,ORF00169, ORF00198, ORF00222, ORF00223, ORF00224, ORF00225, ORF00226,ORF00231, ORF00232, ORF00233, ORF00250, ORF00251, ORF00252, ORF00253,ORF00254, ORF00255, ORF00256, ORF00257, ORF00330, ORF00331, ORF00332,ORF00333, ORF00334, ORF00335, ORF00336, ORF00337, ORF00338, ORF00339,ORF00340, ORF00341, ORF00342, ORF00343, ORF00344, ORF00345, ORF00346,ORF00347, ORF00348, ORF00349, ORF00350, ORF00351, ORF00352, ORF00353,ORF00361, ORF00379, ORF00380, ORF00381, ORF00382, ORF00383, ORF00394,ORF00395, ORF00396, ORF00397, ORF00399, ORF00400, ORF00401, ORF00402,ORF00403, ORF00404, ORF00407, ORF00415, ORF00416, ORF00417, ORF00418,ORF00419, ORF00433, ORF00434, ORF00435, ORF00436, ORF00437, ORF00442,ORF00444, ORF00455, ORF00587, ORF00588, ORF00591, ORF00597, ORF00598,ORF00599, ORF00600, ORF00631, ORF00644, ORF00646, ORF00647, ORF00648,ORF00649, ORF00650, ORF00655, ORF00656, ORF00657, ORF00659, ORF00660,ORF00661, ORF00662, ORF00663, ORF00664, ORF00665, ORF00666, ORF00667,ORF00668, ORF00669, ORF00670, ORF00671, ORF00672, ORF00673, ORF00674,ORF00675, ORF00676, ORF00677, ORF00678, ORF00680, ORF00681, ORF00682,ORF00683, ORF00685, ORF00687, ORF00773, ORF00786, ORF00787, ORF00788,ORF00789, ORF00790, ORF00791, ORF00792, ORF00793, ORF00794, ORF00795,ORF00796, ORF00798, ORF00803, ORF00804, ORF00809, ORF00810, ORF00838,ORF00839, ORF00857, ORF00864, ORF00883, ORF00884, ORF00885, ORF00886,ORF00887, ORF00888, ORF00889, ORF00890, ORF00891, ORF00892, ORF00893,ORF00894, ORF00895, ORF00896, ORF00897, ORF00898, ORF00899, ORF00900,ORF00901, ORF00902, ORF00903, ORF00904, ORF00905, ORF00906, ORF00907,ORF00908, ORF00909, ORF00910, ORF00924, ORF00925, ORF00926, ORF00948,ORF00956, ORF01031, ORF01032, ORF01033, ORF01034, ORF01035, ORF01036,ORF01037, ORF01038, ORF01039, ORF01040, ORF01041, ORF01042, ORF01043,ORF01044, ORF01045, ORF01046, ORF01047, ORF01048, ORF01052, ORF01053,ORF01054, ORF01055, ORF01056, ORF01057, ORF01058, ORF01063, ORF01078,ORF01079, ORF01080, ORF01081, ORF01084, ORF01085, ORF01086, ORF01087,ORF01088, ORF01089, ORF01090, ORF01091, ORF01092, ORF01093, ORF01094,ORF01095, ORF01096, ORF01097, ORF01098, ORF01099, ORF01100, ORF01101,ORF01102, ORF01103, ORF01104, ORF01105, ORF01106, ORF01107, ORF01108,ORF01109, ORF01110, ORF01111, ORF01112, ORF01113, ORF01114, ORF01115,ORF01116, ORF01117, ORF01118, ORF01119, ORF01156, ORF01186, ORF01187,ORF01188, ORF01190, ORF01191, ORF01192, ORF01193, ORF01194, ORF01195,ORF01196, ORF01199, ORF01200, ORF01201, ORF01202, ORF01203, ORF01204,ORF01205, ORF01206, ORF01207, ORF01208, ORF01209, ORF01210, ORF01211,ORF01212, ORF01213, ORF01214, ORF01215, ORF01216, ORF01217, ORF01218,ORF01219, ORF01220, ORF01222, ORF01223, ORF01224, ORF01225, ORF01226,ORF01227, ORF01228, ORF01236, ORF01252, ORF01253, ORF01254, ORF01255,ORF01256, ORF01257, ORF01258, ORF01259, ORF01260, ORF01261, ORF01283,ORF01313, ORF01314, ORF01315, ORF01316, ORF01317, ORF01318, ORF01319,ORF01320, ORF01321, ORF01322, ORF01323, ORF01325, ORF01330, ORF01331,ORF01332, ORF01333, ORF01334, ORF01335, ORF01336, ORF01337, ORF01338,ORF01339, ORF01340, ORF01341, ORF01342, ORF01343, ORF01344, ORF01345,ORF01346, ORF01347, ORF01348, ORF01349, ORF01350, ORF01351, ORF01352,ORF01353, ORF01354, ORF01355, ORF01356, ORF01357, ORF01359, ORF01360,ORF01361, ORF01365, ORF01366, ORF01367, ORF01368, ORF01371, ORF01373,ORF01374, ORF01375, ORF01376, ORF01394, ORF01456, ORF01462, ORF01477,ORF01479, ORF01480, ORF01483, ORF01484, ORF01485, ORF01486, ORF01487,ORF01488, ORF01490, ORF01491, ORF01492, ORF01493, ORF01494, ORF01498,ORF01499, ORF01500, ORF01501, ORF01502, ORF01503, ORF01504, ORF01505,ORF01506, ORF01507, ORF01508, ORF01509, ORF01510, ORF01511, ORF01512,ORF01513, ORF01514, ORF01518, ORF01523, ORF01524, ORF01525, ORF01526,ORF01527, ORF01528, ORF01529, ORF01530, ORF01531, ORF01532, ORF01533,ORF01534, ORF01535, ORF01536, ORF01537, ORF01538, ORF01539, ORF01540,ORF01541, ORF01542, ORF01543, ORF01550, ORF01551, ORF01552, ORF01553,ORF01554, ORF01555, ORF01556, ORF01557, ORF01558, ORF01559, ORF01560,ORF01561, ORF01562, ORF01563, ORF01564, ORF01565, ORF01566, ORF01567,ORF01568, ORF01569, ORF01570, ORF01571, ORF01572, ORF01573, ORF01574,ORF01575, ORF01576, ORF01577, ORF01578, ORF01583, ORF01588, ORF01589,ORF01590, ORF01591, ORF01592, ORF01593, ORF01609, ORF01610, ORF01611,ORF01612, ORF01613, ORF01630, ORF01631, ORF01637, ORF01651, ORF01655,ORF01656, ORF01657, ORF01658, ORF01659, ORF01660, ORF01665, ORF01705,ORF01722, ORF01723, ORF01724, ORF01725, ORF01766, ORF01767, ORF01768,ORF01769, ORF01770, ORF01790, ORF01810, ORF01812, ORF01813, ORF01815,ORF01816, ORF01817, ORF01818, ORF01819, ORF01820, ORF01821, ORF01822,ORF01823, ORF01824, ORF01825, ORF01826, ORF01827, ORF01828, ORF01829,ORF01830, ORF01831, ORF01832, ORF01833, ORF01834, ORF01835, ORF01836,ORF01837, ORF01838, ORF01840, ORF01842, ORF01843, ORF01844, ORF01845,ORF01846, ORF01847, ORF01848, ORF01849, ORF01850, ORF01852, ORF01853,ORF01854, ORF01855, ORF01856, ORF01857, ORF01861, ORF01862, ORF01865,ORF01891, ORF01923, ORF01926, ORF01927, ORF01928, ORF01929, ORF01930,ORF01931, ORF01932, ORF01946, ORF01953, ORF01955, ORF01956, ORF01987,ORF02112, ORF02266, ORF02289, ORF02295, ORF02296, ORF02297, ORF02298,ORF02299, ORF02300, ORF02301, ORF02302, ORF02303, ORF02304, ORF02305,ORF02306, ORF02307, ORF02308, ORF02309, ORF02310, ORF02311, ORF02312,ORF02313, ORF02314, ORF02315, ORF02327, ORF02338, ORF02351, ORF02352,ORF02353, ORF02354, ORF02355, ORF02356, ORF02358, ORF02359, ORF02361,ORF02362, ORF02363, ORF02364, ORF02365, ORF02366, ORF02367, ORF02368,ORF02369, ORF02370, ORF02371, ORF02372, ORF02373, ORF02374, ORF02375,ORF02382, ORF02383, ORF02384, ORF02385, ORF02386, ORF02387, ORF02388,ORF02389, ORF02390, ORF02392, ORF02393, ORF02394, ORF02395, ORF02396,ORF02397, ORF02398, ORF02399, ORF02400, ORF02401, ORF02402, ORF02403,ORF02404, ORF02405, ORF02413, ORF02414, ORF02415, ORF02419, ORF02420,ORF02421, ORF02422, ORF02423, ORF02424, ORF02425, ORF02429, ORF02432,ORF02436, ORF02437, ORF02438, ORF02439, ORF02440, ORF02441, ORF02442,ORF02443, ORF02444, ORF02445, ORF02446, ORF02447, ORF02448, ORF02449,ORF02450, ORF02451, ORF02452, ORF02453, ORF02454, ORF02455, ORF02456,ORF02457, ORF02458, ORF02459, ORF02460, ORF02461, ORF02462, ORF02464,ORF02465, ORF02466, ORF02467, ORF02468, ORF02469, ORF02470, ORF02471,ORF02472, ORF02501, ORF02502, ORF02503, ORF02504, ORF02505, ORF02506,ORF02507, ORF02533, ORF02534, ORF02552, ORF02553, ORF02554, ORF02555,ORF02582, ORF02583, ORF02595, ORF02614, ORF02628, ORF02633, ORF02699,ORF02735, ORF02788, ORF02789, ORF02790, ORF02791, ORF02792, ORF02793,ORF02794, ORF02795, ORF02796, ORF02797, ORF02798, ORF02799, ORF02800,ORF02801, ORF02802, ORF02803, ORF02804, ORF02805, ORF02806, ORF02807,ORF02808, ORF02809, ORF02810, ORF02811, ORF02812, ORF02813, ORF02814,ORF02815, ORF02816, ORF02828, ORF02829, ORF02830, ORF02831, ORF02832,ORF02833, ORF02834, ORF02835, ORF02844, ORF02845, ORF02846, ORF02847,ORF02848, ORF02849, ORF02850, ORF02851, ORF02852, ORF02853, ORF02854,ORF02855, ORF02856, ORF02857, ORF02858, ORF02859, ORF02860, ORF02861,ORF02862, ORF02863, ORF02864, ORF02865, ORF02866, ORF02867, ORF02868,ORF02869, ORF02870, ORF02871, ORF02872, ORF02873, ORF02874, ORF02875,ORF02876, ORF02877, ORF02878, ORF02879, ORF02880, ORF02881, ORF02882,ORF02883, ORF02884, ORF02885, ORF02886, ORF02887, ORF02888, ORF02889,ORF02890, ORF02891, ORF02931, ORF03011, ORF03012, ORF03013, ORF03014,ORF03015, ORF03016, ORF03017, ORF03018, ORF03019, ORF03020, ORF03021,ORF03022, ORF03023, ORF03024, ORF03025, ORF03026, ORF03027, ORF03028,ORF03029, ORF03030, ORF03031, ORF03032, ORF03033, ORF03034, ORF03035,ORF03036, ORF03037, ORF03038, ORF03039, ORF03040, ORF03041, ORF03042,ORF03043, ORF03044, ORF03045, ORF03046, ORF03047, ORF03048, ORF03049,ORF03053, ORF03054, ORF03055, ORF03056, ORF03114, ORF03130, ORF03131,ORF03132, ORF03133, ORF03162, ORF03163, ORF03164, ORF03165, ORF03166,ORF03167, ORF03173, ORF03174, ORF03175, ORF03176, ORF03191, ORF03192,ORF03206, ORF03207, ORF03235, ORF03236, ORF03252, ORF03262, ORF03263,ORF03279, ORF03296, ORF03297, ORF03298, ORF03299, ORF03300, ORF03325,ORF03340, ORF03341, ORF03342, ORF03343, ORF03344, ORF03345, ORF03346,ORF03347, ORF03348, ORF03349, ORF03350, ORF03351, ORF03352, ORF03353,ORF03354, ORF03355, ORF03356, ORF03357, ORF03358, ORF03359, ORF03360,ORF03361, ORF03362, ORF03363, ORF03364, ORF03365, ORF03400, ORF03415,ORF03453, ORF03454, ORF03455, ORF03456, ORF03457, ORF03458, ORF03459,ORF03465, ORF03469, ORF03499, ORF03500, ORF03501, ORF03502, ORF03503,ORF03504, ORF03505, ORF03506, ORF03507, ORF03508, ORF03509, ORF03510,ORF03511, ORF03512, ORF03515, ORF03516, ORF03517, ORF03518, ORF03519,ORF03520, ORF03521, ORF03522, ORF03526, ORF03535, ORF03536, ORF03537,ORF03538, ORF03539, ORF03540, ORF03541, ORF03542, ORF03574, ORF03575,ORF03576, ORF03577, ORF03578, ORF03579, ORF03580, ORF03589, ORF03592,ORF03593, ORF03594, ORF03595, ORF03596, ORF03597, ORF03606, ORF03607,ORF03608, ORF03610, ORF03612, ORF03613, ORF03614, ORF03615, ORF03616,ORF03617, ORF03619, ORF03695, ORF03696, ORF03817, ORF03818, ORF03819,ORF03820, ORF03821, ORF03822, ORF03823, ORF03824, ORF03825, ORF03826,ORF03975, ORF03991, ORF03992, ORF03993, ORF03994, ORF03995, ORF03996,ORF03997, ORF03998, ORF03999, ORF04000, ORF04031, ORF04032, ORF04033,ORF04034, ORF04035, ORF04036, ORF04058, ORF04059, ORF04060, ORF04061,ORF04062, ORF04063, ORF04064, ORF04073, ORF04083, ORF04084, ORF04085,ORF04086, ORF04087, ORF04088, ORF04089, ORF04090, ORF04091, ORF04130,ORF04162, ORF04163, ORF04164, ORF04171, ORF04183, ORF04184, ORF04203,ORF04204, ORF04205, ORF04206, ORF04207, ORF04208, ORF04209, ORF04211,ORF04224, ORF04225, ORF04237, ORF04238, ORF04239, ORF04240, ORF04241,ORF04242, ORF04243, ORF04244, ORF04247, ORF04250, ORF04282, ORF04302,ORF04390, ORF04391, ORF04398, ORF04402, ORF04403, ORF04404, ORF04416,ORF04417, ORF04418, ORF04419, ORF04420, ORF04421, ORF04422, ORF04423,ORF04424, ORF04425, ORF04426, ORF04427, ORF04428, ORF04429, ORF04430,ORF04434, ORF04435, ORF04436, ORF04437, ORF04438, ORF04439, ORF04451,ORF04476, ORF04477, ORF04478, ORF04479, ORF04480, ORF04481, ORF04482,ORF04483, ORF04484, ORF04489, ORF04490, ORF04500, ORF04542, ORF04543,ORF04544, ORF04545, ORF04546, ORF04547, ORF04552, ORF04569, ORF04572,ORF04573, ORF04608, ORF04612, ORF04623, ORF04626, ORF04627, ORF04628,ORF04629, ORF04630, ORF04631, ORF04632, ORF04661, ORF04662, ORF04663,ORF04664, ORF04665, ORF04668, ORF04669, ORF04671, ORF04672, ORF04673,ORF04674, ORF04675, ORF04676, ORF04677, ORF04678, ORF04679, ORF04691,ORF04692, ORF04693, ORF04694, ORF04708, ORF04709, ORF04710, ORF04711,ORF04712, ORF04728, ORF04844, ORF04845, ORF04846, ORF04847, ORF04848,ORF04849, ORF04850, ORF04851, ORF04882, ORF04890, ORF04891, ORF04892,ORF04893, ORF04894, ORF04895, ORF04898, ORF04900, ORF04915, ORF04916,ORF04917, ORF04918, ORF04919, ORF04920, ORF04921, ORF04922, ORF04923,ORF04924, ORF04925, ORF04926, ORF04927, ORF04928, ORF04929, ORF04930,ORF04946, ORF04947, ORF04948, ORF04949, ORF04950, ORF04951, ORF04952,ORF04953, ORF04954, ORF04955, ORF04956, ORF04957, ORF04958, ORF04959,ORF04960, ORF04961, ORF04962, ORF04963, ORF04964, ORF04966, ORF04974,ORF04978, ORF04979, ORF04981, ORF04989, ORF04990, ORF04991, ORF04992,ORF04993, ORF04995

TABLE 4 Protein SEQ ID Annotation Psort TMD CFT073 % ID ORF03526 7051 +7052 Accessory colonization factor AcfD precursor I 0 upec-2704 45.3ORF01339 2677 + 2678 F1C fimbrial usher I 1 upec-1199 99.4 ORF00256 511 + 512 pilin chaperone ecpD2 I 1 upec-0166 100 ORF01346 2691 + 2692IroN protein O 0 upec-1207 99.3 ORF04084 8167 + 8168 outer membranehemin receptor ChuA O 0 upec-4213 99.7 ORF02374 4747 + 4748 OMR familypesticin-yersiniabactin O 0 upec-2371 100 receptor protein (IRPC)ORF03502 7003 + 7004 polysaccharide biosynthesis protein, O 0 upec-360699.5 putative ORF02298 4595 + 4596 hypothetical protein C 0 upec-5009 30ORF01228 2455 + 2456 CdtC O 0 upec-1086 30.3 ORF01227 2453 + 2454 CdtB I1 upec-0679 31.9 ORF02314 4627 + 4628 gpH I 1 upec-0941 48.8 ORF028505699 + 5700 DNA transfer protein C 0 upec-4881 21.2 Columns: PSORT:predicted location by the PSORT algorithm. I = inner membrane; O = outermembrane; P = periplasm; C = cytoplasm TMD: number of transmembranedomains % ID: percentage identitical residues over length of alignment

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1. (canceled)
 2. A polypeptide comprising: (a) an amino acid sequenceselected from the group consisting of SEQ ID NOs 706, 2, 4, 6, 8, 10,12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46,48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82,84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114,116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142,144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170,172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198,200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226,228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254,256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282,284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310,312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338,340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366,368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394,396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422,424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450,452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478,480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506,508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534,536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562,564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590,592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618,620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646,648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674,676, 678, 680, 682, 684, 686, 688, 690, 692, 694, 696, 698, 700, 702,704, 708, 710, 712, 714, 716, 718, 720, 722, 724, 726, 728, 730, 732,734, 736, 738, 740, 742, 744, 746, 748, 750, 752, 754, 756, 758, 760,762, 764, 766, 768, 770, 772, 774, 776, 778, 780, 782, 784, 786, 788,790, 792, 794, 796, 798, 800, 802, 804, 806, 808, 810, 812, 814, 816,818, 820, 822, 824, 826, 828, 830, 832, 834, 836, 838, 840, 842, 844,846, 848, 850, 852, 854, 856, 858, 860, 862, 864, 866, 868, 870, 872,874, 876, 878, 880, 882, 884, 886, 888, 890, 892, 894, 896, 898, 900,902, 904, 906, 908, 910, 912, 914, 916, 918, 920, 922, 924, 926, 928,930, 932, 934, 936, 938, 940, 942, 944, 946, 948, 950, 952, 954, 956,958, 960, 962, 964, 966, 968, 970, 972, 974, 976, 978, 980, 982, 984,986, 988, 990, 992, 994, 996, 998, 1000, 1002, 1004, 1006, 1008, 1010,1012, 1014, 1016, 1018, 1020, 1022, 1024, 1026, 1028, 1030, 1032, 1034,1036, 1038, 1040, 1042, 1044, 1046, 1048, 1050, 1052, 1054, 1056, 1058,1060, 1062, 1064, 1066, 1068, 1070, 1072, 1074, 1076, 1078, 1080, 1082,1084, 1086, 1088, 1090, 1092, 1094, 1096, 1098, 1100, 1102, 1104, 1106,1108, 1110, 1112, 1114, 1116, 1118, 1120, 1122, 1124, 1126, 1128, 1130,1132, 1134, 1136, 1138, 1140, 1142, 1144, 1146, 1148, 1150, 1152, 1154,1156, 1158, 1160, 1162, 1164, 1166, 1168, 1170, 1172, 1174, 1176, 1178,1180, 1182, 1184, 1186, 1188, 1190, 1192, 1194, 1196, 1198, 1200, 1202,1204, 1206, 1208, 1210, 1212, 1214, 1216, 1218, 1220, 1222, 1224, 1226,1228, 1230, 1232, 1234, 1236, 1238, 1240, 1242, 1244, 1246, 1248, 1250,1252, 1254, 1256, 1258, 1260, 1262, 1264, 1266, 1268, 1270, 1272, 1274,1276, 1278, 1280, 1282, 1284, 1286, 1288, 1290, 1292, 1294, 1296, 1298,1300, 1302, 1304, 1306, 1308, 1310, 1312, 1314, 1316, 1318, 1320, 1322,1324, 1326, 1328, 1330, 1332, 1334, 1336, 1338, 1340, 1342, 1344, 1346,1348, 1350, 1352, 1354, 1356, 1358, 1360, 1362, 1364, 1366, 1368, 1370,1372, 1374, 1376, 1378, 1380, 1382, 1384, 1386, 1388, 1390, 1392, 1394,1396, 1398, 1400, 1402, 1404, 1406, 1408, 1410, 1412, 1414, 1416, 1418,1420, 1422, 1424, 1426, 1428, 1430, 1432, 1434, 1436, 1438, 1440, 1442,1444, 1446, 1448, 1450, 1452, 1454, 1456, 1458, 1460, 1462, 1464, 1466,1468, 1470, 1472, 1474, 1476, 1478, 1480, 1482, 1484, 1486, 1488, 1490,1492, 1494, 1496, 1498, 1500, 1502, 1504, 1506, 1508, 1510, 1512, 1514,1516, 1518, 1520, 1522, 1524, 1526, 1528, 1530, 1532, 1534, 1536, 1538,1540, 1542, 1544, 1546, 1548, 1550, 1552, 1554, 1556, 1558, 1560, 1562,1564, 1566, 1568, 1570, 1572, 1574, 1576, 1578, 1580, 1582, 1584, 1586,1588, 1590, 1592, 1594, 1596, 1598, 1600, 1602, 1604, 1606, 1608, 1610,1612, 1614, 1616, 1618, 1620, 1622, 1624, 1626, 1628, 1630, 1632, 1634,1636, 1638, 1640, 1642, 1644, 1646, 1648, 1650, 1652, 1654, 1656, 1658,1660, 1662, 1664, 1666, 1668, 1670, 1672, 1674, 1676, 1678, 1680, 1682,1684, 1686, 1688, 1690, 1692, 1694, 1696, 1698, 1700, 1702, 1704, 1706,1708, 1710, 1712, 1714, 1716, 1718, 1720, 1722, 1724, 1726, 1728, 1730,1732, 1734, 1736, 1738, 1740, 1742, 1744, 1746, 1748, 1750, 1752, 1754,1756, 1758, 1760, 1762, 1764, 1766, 1768, 1770, 1772, 1774, 1776, 1778,1780, 1782, 1784, 1786, 1788, 1790, 1792, 1794, 1796, 1798, 1800, 1802,1804, 1806, 1808, 1810, 1812, 1814, 1816, 1818, 1820, 1822, 1824, 1826,1828, 1830, 1832, 1834, 1836, 1838, 1840, 1842, 1844, 1846, 1848, 1850,1852, 1854, 1856, 1858, 1860, 1862, 1864, 1866, 1868, 1870, 1872, 1874,1876, 1878, 1880, 1882, 1884, 1886, 1888, 1890, 1892, 1894, 1896, 1898,1900, 1902, 1904, 1906, 1908, 1910, 1912, 1914, 1916, 1918, 1920, 1922,1924, 1926, 1928, 1930, 1932, 1934, 1936, 1938, 1940, 1942, 1944, 1946,1948, 1950, 1952, 1954, 1956, 1958, 1960, 1962, 1964, 1966, 1968, 1970,1972, 1974, 1976, 1978, 1980, 1982, 1984, 1986, 1988, 1990, 1992, 1994,1996, 1998, 2000, 2002, 2004, 2006, 2008, 2010, 2012, 2014, 2016, 2018,2020, 2022, 2024, 2026, 2028, 2030, 2032, 2034, 2036, 2038, 2040, 2042,2044, 2046, 2048, 2050, 2052, 2054, 2056, 2058, 2060, 2062, 2064, 2066,2068, 2070, 2072, 2074, 2076, 2078, 2080, 2082, 2084, 2086, 2088, 2090,2092, 2094, 2096, 2098, 2100, 2102, 2104, 2106, 2108, 2110, 2112, 2114,2116, 2118, 2120, 2122, 2124, 2126, 2128, 2130, 2132, 2134, 2136, 2138,2140, 2142, 2144, 2146, 2148, 2150, 2152, 2154, 2156, 2158, 2160, 2162,2164, 2166, 2168, 2170, 2172, 2174, 2176, 2178, 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6218,6220, 6222, 6224, 6226, 6228, 6230, 6232, 6234, 6236, 6238, 6240, 6242,6244, 6246, 6248, 6250, 6252, 6254, 6256, 6258, 6260, 6262, 6264, 6266,6268, 6270, 6272, 6274, 6276, 6278, 6280, 6282, 6284, 6286, 6288, 6290,6292, 6294, 6296, 6298, 6300, 6302, 6304, 6306, 6308, 6310, 6312, 6314,6316, 6318, 6320, 6322, 6324, 6326, 6328, 6330, 6332, 6334, 6336, 6338,6340, 6342, 6344, 6346, 6348, 6350, 6352, 6354, 6356, 6358, 6360, 6362,6364, 6366, 6368, 6370, 6372, 6374, 6376, 6378, 6380, 6382, 6384, 6386,6388, 6390, 6392, 6394, 6396, 6398, 6400, 6402, 6404, 6406, 6408, 6410,6412, 6414, 6416, 6418, 6420, 6422, 6424, 6426, 6428, 6430, 6432, 6434,6436, 6438, 6440, 6442, 6444, 6446, 6448, 6450, 6452, 6454, 6456, 6458,6460, 6462, 6464, 6466, 6468, 6470, 6472, 6474, 6476, 6478, 6480, 6482,6484, 6486, 6488, 6490, 6492, 6494, 6496, 6498, 6500, 6502, 6504, 6506,6508, 6510, 6512, 6514, 6516, 6518, 6520, 6522, 6524, 6526, 6528, 6530,6532, 6534, 6536, 6538, 6540, 6542, 6544, 6546, 6548, 6550, 6552, 6554,6556, 6558, 6560, 6562, 6564, 6566, 6568, 6570, 6572, 6574, 6576, 6578,6580, 6582, 6584, 6586, 6588, 6590, 6592, 6594, 6596, 6598, 6600, 6602,6604, 6606, 6608, 6610, 6612, 6614, 6616, 6618, 6620, 6622, 6624, 6626,6628, 6630, 6632, 6634, 6636, 6638, 6640, 6642, 6644, 6646, 6648, 6650,6652, 6654, 6656, 6658, 6660, 6662, 6664, 6666, 6668, 6670, 6672, 6674,6676, 6678, 6680, 6682, 6684, 6686, 6688, 6690, 6692, 6694, 6696, 6698,6700, 6702, 6704, 6706, 6708, 6710, 6712, 6714, 6716, 6718, 6720, 6722,6724, 6726, 6728, 6730, 6732, 6734, 6736, 6738, 6740, 6742, 6744, 6746,6748, 6750, 6752, 6754, 6756, 6758, 6760, 6762, 6764, 6766, 6768, 6770,6772, 6774, 6776, 6778, 6780, 6782, 6784, 6786, 6788, 6790, 6792, 6794,6796, 6798, 6800, 6802, 6804, 6806, 6808, 6810, 6812, 6814, 6816, 6818,6820, 6822, 6824, 6826, 6828, 6830, 6832, 6834, 6836, 6838, 6840, 6842,6844, 6846, 6848, 6850, 6852, 6854, 6856, 6858, 6860, 6862, 6864, 6866,6868, 6870, 6872, 6874, 6876, 6878, 6880, 6882, 6884, 6886, 6888, 6890,6892, 6894, 6896, 6898, 6900, 6902, 6904, 6906, 6908, 6910, 6912, 6914,6916, 6918, 6920, 6922, 6924, 6926, 6928, 6930, 6932, 6934, 6936, 6938,6940, 6942, 6944, 6946, 6948, 6950, 6952, 6954, 6956, 6958, 6960, 6962,6964, 6966, 6968, 6970, 6972, 6974, 6976, 6978, 6980, 6982, 6984, 6986,6988, 6990, 6992, 6994, 6996, 6998, 7000, 7002, 7004, 7006, 7008, 7010,7012, 7014, 7016, 7018, 7020, 7022, 7024, 7026, 7028, 7030, 7032, 7034,7036, 7038, 7040, 7042, 7044, 7046, 7048, 7050, 7054, 7056, 7058, 7060,7062, 7064, 7066, 7068, 7070, 7072, 7074, 7076, 7078, 7080, 7082, 7084,7086, 7088, 7090, 7092, 7094, 7096, 7098, 7100, 7102, 7104, 7106, 7108,7110, 7112, 7114, 7116, 7118, 7120, 7122, 7124, 7126, 7128, 7130, 7132,7134, 7136, 7138, 7140, 7142, 7144, 7146, 7148, 7150, 7152, 7154, 7156,7158, 7160, 7162, 7164, 7166, 7168, 7170, 7172, 7174, 7176, 7178, 7180,7182, 7184, 7186, 7188, 7190, 7192, 7194, 7196, 7198, 7200, 7202, 7204,7206, 7208, 7210, 7212, 7214, 7216, 7218, 7220, 7222, 7224, 7226, 7228,7230, 7232, 7234, 7236, 7238, 7240, 7242, 7244, 7246, 7248, 7250, 7252,7254, 7256, 7258, 7260, 7262, 7264, 7266, 7268, 7270, 7272, 7274, 7276,7278, 7280, 7282, 7284, 7286, 7288, 7290, 7292, 7294, 7296, 7298, 7300,7302, 7304, 7306, 7308, 7310, 7312, 7314, 7316, 7318, 7320, 7322, 7324,7326, 7328, 7330, 7332, 7334, 7336, 7338, 7340, 7342, 7344, 7346, 7348,7350, 7352, 7354, 7356, 7358, 7360, 7362, 7364, 7366, 7368, 7370, 7372,7374, 7376, 7378, 7380, 7382, 7384, 7386, 7388, 7390, 7392, 7394, 7396,7398, 7400, 7402, 7404, 7406, 7408, 7410, 7412, 7414, 7416, 7418, 7420,7422, 7424, 7426, 7428, 7430, 7432, 7434, 7436, 7438, 7440, 7442, 7444,7446, 7448, 7450, 7452, 7454, 7456, 7458, 7460, 7462, 7464, 7466, 7468,7470, 7472, 7474, 7476, 7478, 7480, 7482, 7484, 7486, 7488, 7490, 7492,7494, 7496, 7498, 7500, 7502, 7504, 7506, 7508, 7510, 7512, 7514, 7516,7518, 7520, 7522, 7524, 7526, 7528, 7530, 7532, 7534, 7536, 7538, 7540,7542, 7544, 7546, 7548, 7550, 7552, 7554, 7556, 7558, 7560, 7562, 7564,7566, 7568, 7570, 7572, 7574, 7576, 7578, 7580, 7582, 7584, 7586, 7588,7590, 7592, 7594, 7596, 7598, 7600, 7602, 7604, 7606, 7608, 7610, 7612,7614, 7616, 7618, 7620, 7622, 7624, 7626, 7628, 7630, 7632, 7634, 7636,7638, 7640, 7642, 7644, 7646, 7648, 7650, 7652, 7654, 7656, 7658, 7660,7662, 7664, 7666, 7668, 7670, 7672, 7674, 7676, 7678, 7680, 7682, 7684,7686, 7688, 7690, 7692, 7694, 7696, 7698, 7700, 7702, 7704, 7706, 7708,7710, 7712, 7714, 7716, 7718, 7720, 7722, 7724, 7726, 7728, 7730, 7732,7734, 7736, 7738, 7740, 7742, 7744, 7746, 7748, 7750, 7752, 7754, 7756,7758, 7760, 7762, 7764, 7766, 7768, 7770, 7772, 7774, 7776, 7778, 7780,7782, 7784, 7786, 7788, 7790, 7792, 7794, 7796, 7798, 7800, 7802, 7804,7806, 7808, 7810, 7812, 7814, 7816, 7818, 7820, 7822, 7824, 7826, 7828,7830, 7832, 7834, 7836, 7838, 7840, 7842, 7844, 7846, 7848, 7850, 7852,7854, 7856, 7858, 7860, 7862, 7864, 7866, 7868, 7870, 7872, 7874, 7876,7878, 7880, 7882, 7884, 7886, 7888, 7890, 7892, 7894, 7896, 7898, 7900,7902, 7904, 7906, 7908, 7910, 7912, 7914, 7916, 7918, 7920, 7922, 7924,7926, 7928, 7930, 7932, 7934, 7936, 7938, 7940, 7942, 7944, 7946, 7948,7950, 7952, 7954, 7956, 7958, 7960, 7962, 7964, 7966, 7968, 7970, 7972,7974, 7976, 7978, 7980, 7982, 7984, 7986, 7988, 7990, 7992, 7994, 7996,7998, 8000, 8002, 8004, 8006, 8008, 8010, 8012, 8014, 8016, 8018, 8020,8022, 8024, 8026, 8028, 8030, 8032, 8034, 8036, 8038, 8040, 8042, 8044,8046, 8048, 8050, 8052, 8054, 8056, 8058, 8060, 8062, 8064, 8066, 8068,8070, 8072, 8074, 8076, 8078, 8080, 8082, 8084, 8086, 8088, 8090, 8092,8094, 8096, 8098, 8100, 8102, 8104, 8106, 8108, 8110, 8112, 8114, 8116,8118, 8120, 8122, 8124, 8126, 8128, 8130, 8132, 8134, 8136, 8138, 8140,8142, 8144, 8146, 8148, 8150, 8152, 8154, 8156, 8158, 8160, 8162, 8164,8166, 8168, 8170, 8172, 8174, 8176, 8178, 8180, 8182, 8184, 8186, 8188,8190, 8192, 8194, 8196, 8198, 8200, 8202, 8204, 8206, 8208, 8210, 8212,8214, 8216, 8218, 8220, 8222, 8224, 8226, 8228, 8230, 8232, 8234, 8236,8238, 8240, 8242, 8244, 8246, 8248, 8250, 8252, 8254, 8256, 8258, 8260,8262, 8264, 8266, 8268, 8270, 8272, 8274, 8276, 8278, 8280, 8282, 8284,8286, 8288, 8290, 8292, 8294, 8296, 8298, 8300, 8302, 8304, 8306, 8308,8310, 8312, 8314, 8316, 8318, 8320, 8322, 8324, 8326, 8328, 8330, 8332,8334, 8336, 8338, 8340, 8342, 8344, 8346, 8348, 8350, 8352, 8354, 8356,8358, 8360, 8362, 8364, 8366, 8368, 8370, 8372, 8374, 8376, 8378, 8380,8382, 8384, 8386, 8388, 8390, 8392, 8394, 8396, 8398, 8400, 8402, 8404,8406, 8408, 8410, 8412, 8414, 8416, 8418, 8420, 8422, 8424, 8426, 8428,8430, 8432, 8434, 8436, 8438, 8440, 8442, 8444, 8446, 8448, 8450, 8452,8454, 8456, 8458, 8460, 8462, 8464, 8466, 8468, 8470, 8472, 8474, 8476,8478, 8480, 8482, 8484, 8486, 8488, 8490, 8492, 8494, 8496, 8498, 8500,8502, 8504, 8506, 8508, 8510, 8512, 8514, 8516, 8518, 8520, 8522, 8524,8526, 8528, 8530, 8532, 8534, 8536, 8538, 8540, 8542, 8544, 8546, 8548,8550, 8552, 8554, 8556, 8558, 8560, 8562, 8564, 8566, 8568, 8570, 8572,8574, 8576, 8578, 8580, 8582, 8584, 8586, 8588, 8590, 8592, 8594, 8596,8598, 8600, 8602, 8604, 8606, 8608, 8610, 8612, 8614, 8616, 8618, 8620,8622, 8624, 8626, 8628, 8630, 8632, 8634, 8636, 8638, 8640, 8642, 8644,8646, 8648, 8650, 8652, 8654, 8656, 8658, 8660, 8662, 8664, 8666, 8668,8670, 8672, 8674, 8676, 8678, 8680, 8682, 8684, 8686, 8688, 8690, 8692,8694, 8696, 8698, 8700, 8702, 8704, 8706, 8708, 8710, 8712, 8714, 8716,8718, 8720, 8722, 8724, 8726, 8728, 8730, 8732, 8734, 8736, 8738, 8740,8742, 8744, 8746, 8748, 8750, 8752, 8754, 8756, 8758, 8760, 8762, 8764,8766, 8768, 8770, 8772, 8774, 8776, 8778, 8780, 8782, 8784, 8786, 8788,8790, 8792, 8794, 8796, 8798, 8800, 8802, 8804, 8806, 8808, 8810, 8812,8814, 8816, 8818, 8820, 8822, 8824, 8826, 8828, 8830, 8832, 8834, 8836,8838, 8840, 8842, 8844, 8846, 8848, 8850, 8852, 8854, 8856, 8858, 8860,8862, 8864, 8866, 8868, 8870, 8872, 8874, 8876, 8878, 8880, 8882, 8884,8886, 8888, 8890, 8892, 8894, 8896, 8898, 8900, 8902, 8904, 8906, 8908,8910, 8912, 8914, 8916, 8918, 8920, 8922, 8924, 8926, 8928, 8930, 8932,8934, 8936, 8938, 8940, 8942, 8944, 8946, 8948, 8950, 8952, 8954, 8956,8958, 8960, 8962, 8964, 8966, 8968, 8970, 8972, 8974, 8976, 8978, 8980,8982, 8984, 8986, 8988, 8990, 8992, 8994, 8996, 8998, 9000, 9002, 9004,9006, 9008, 9010, 9012, 9014, 9016, 9018, 9020, 9022, 9024, 9026, 9028,9030, 9032, 9034, 9036, 9038, 9040, 9042, 9044, 9046, 9048, 9050, 9052,9054, 9056, 9058, 9060, 9062, 9064, 9066, 9068, 9070, 9072, 9074, 9076,9078, 9080, 9082, 9084, 9086, 9088, 9090, 9092, 9094, 9096, 9098, 9100,9102, 9104, 9106, 9108, 9110, 9112, 9114, 9116, 9118, 9120, 9122, 9124,9126, 9128, 9130, 9132, 9134, 9136, 9138, 9140, 9142, 9144, 9146, 9148,9150, 9152, 9154, 9156, 9158, 9160, 9162, 9164, 9166, 9168, 9170, 9172,9174, 9176, 9178, 9180, 9182, 9184, 9186, 9188, 9190, 9192, 9194, 9196,9198, 9200, 9202, 9204, 9206, 9208, 9210, 9212, 9214, 9216, 9218, 9220,9222, 9224, 9226, 9228, 9230, 9232, 9234, 9236, 9238, 9240, 9242, 9244,9246, 9248, 9250, 9252, 9254, 9256, 9258, 9260, 9262, 9264, 9266, 9268,9270, 9272, 9274, 9276, 9278, 9280, 9282, 9284, 9286, 9288, 9290, 9292,9294, 9296, 9298, 9300, 9302, 9304, 9306, 9308, 9310, 9312, 9314, 9316,9318, 9320, 9322, 9324, 9326, 9328, 9330, 9332, 9334, 9336, 9338, 9340,9342, 9344, 9346, 9348, 9350, 9352, 9354, 9356, 9358, 9360, 9362, 9364,9366, 9368, 9370, 9372, 9374, 9376, 9378, 9380, 9382, 9384, 9386, 9388,9390, 9392, 9394, 9396, 9398, 9400, 9402, 9404, 9406, 9408, 9410, 9412,9414, 9416, 9418, 9420, 9422, 9424, 9426, 9428, 9430, 9432, 9434, 9436,9438, 9440, 9442, 9444, 9446, 9448, 9450, 9452, 9454, 9456, 9458, 9460,9462, 9464, 9466, 9468, 9470, 9472, 9474, 9476, 9478, 9480, 9482, 9484,9486, 9488, 9490, 9492, 9494, 9496, 9498, 9500, 9502, 9504, 9506, 9508,9510, 9512, 9514, 9516, 9518, 9520, 9522, 9524, 9526, 9528, 9530, 9532,9534, 9536, 9538, 9540, 9542, 9544, 9546, 9548, 9550, 9552, 9554, 9556,9558, 9560, 9562, 9564, 9566, 9568, 9570, 9572, 9574, 9576, 9578, 9580,9582, 9584, 9586, 9588, 9590, 9592, 9594, 9596, 9598, 9600, 9602, 9604,9606, 9608, 9610, 9612, 9614, 9616, 9618, 9620, 9622, 9624, 9626, 9628,9630, 9632, 9634, 9636, 9638, 9640, 9642, 9644, 9646, 9648, 9650, 9652,9654, 9656, 9658, 9660, 9662, 9664, 9666, 9668, 9670, 9672, 9674, 9676,9678, 9680, 9682, 9684, 9686, 9688, 9690, 9692, 9694, 9696, 9698, 9700,9702, 9704, 9706, 9708, 9710, 9712, 9714, 9716, 9718, 9720, 9722, 9724,9726, 9728, 9730, 9732, 9734, 9736, 9738, 9740, 9742, 9744, 9746, 9748,9750, 9752, 9754, 9756, 9758, 9760, 9762, 9764, 9766, 9768, 9770, 9772,9774, 9776, 9778, 9780, 9782, 9784, 9786, 9788, 9790, 9792, 9794, 9796,9798, 9800, 9802, 9804, 9806, 9808, 9810, 9812, 9814, 9816, 9818, 9820,9822, 9824, 9826, 9828, 9830, 9832, 9834, 9836, 9838, 9840, 9842, 9844,9846, 9848, 9850, 9852, 9854, 9856, 9858, 9860, 9862, 9864, 9866, 9868,9870, 9872, 9874, 9876, 9878, 9880, 9882, 9884, 9886, 9888, 9890, 9892,9894, 9896, 9898, 9900, 9902, 9904, 9906, 9908, 9910, 9912, 9914, 9916,9918, 9920, 9922, 9924, 9926, 9928, 9930, 9932, 9934, 9936, 9938, 9940,9942, 9944, 9946, 9948, 9950, 9952, 9954, 9956, 9958, 9960, 9962, 9964,9966, 9968, 9970, 9972, 9974, 9976, 9978, 9980, 9982, 9984, 9986, 9988and 9990; (b) an amino acid sequence having at least 80% sequenceidentity to an amino acid sequence of (a); (c) an amino acid sequencewhich is a fragment of at least 10 consecutive amino acids from an aminoacid sequence of (a); or (d) an amino acid sequence having at least 80%sequence identity to an amino acid sequence of (a) and including afragment of at least 10 consecutive amino acids from an amino acidsequence of (a).
 3. The polypeptide of claim 2, wherein said fragmentcomprises at least one B-cell epitope of the corresponding amino acidsequence of (a).
 4. A polynucleotide comprising: (a) a nucleotidesequence selected from the group consisting of SEQ ID NOs 705, 1, 3, 5,7, 9, 11, 13, 15, 17, 19,21, 23, 25,27, 29, 31, 33, 35, 37, 39, 41, 43,45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79,81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111,113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139,141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167,169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195,197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223,225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251,253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279,281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307,309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333, 335,337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359, 361, 363,365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387, 389, 391,393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415, 417, 419,421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447,449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475,477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503,505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531,533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559,561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587,589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615,617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643,645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671,673, 675, 677, 679, 681, 683, 685, 687, 689, 691, 693, 695, 697, 699,701, 703, 707, 709, 711, 713, 715, 717, 719, 721, 723, 725, 727, 729,731, 733, 735, 737, 739, 741, 743, 745, 747, 749, 751, 753, 755, 757,759, 761, 763, 765, 767, 769, 771, 773, 775, 777, 779, 781, 783, 785,787, 789, 791, 793, 795, 797, 799, 801, 803, 805, 807, 809, 811, 813,815, 817, 819, 821, 823, 825, 827, 829, 831, 833, 835, 837, 839, 841,843, 845, 847, 849, 851, 853, 855, 857, 859, 861, 863, 865, 867, 869,871, 873, 875, 877, 879, 881, 883, 885, 887, 889, 891, 893, 895, 897,899, 901, 903, 905, 907, 909, 911, 913, 915, 917, 919, 921, 923, 925,927, 929, 931, 933, 935, 937, 939, 941, 943, 945, 947, 949, 951, 953,955, 957, 959, 961, 963, 965, 967, 969, 971, 973, 975, 977, 979, 981,983, 985, 987, 989, 991, 993, 995, 997, 999, 1001, 1003, 1005, 1007,1009, 1011, 1013, 1015, 1017, 1019, 1021, 1023, 1025, 1027, 1029, 1031,1033, 1035, 1037, 1039, 1041, 1043, 1045, 1047, 1049, 1051, 1053, 1055,1057, 1059, 1061, 1063, 1065, 1067, 1069, 1071, 1073, 1075, 1077, 1079,1081, 1083, 1085, 1087, 1089, 1091, 1093, 1095, 1097, 1099, 1101, 1103,1105, 1107, 1109, 1111, 1113, 1115, 1117, 1119, 1121, 1123, 1125, 1127,1129, 1131, 1133, 1135, 1137, 1139, 1141, 1143, 1145, 1147, 1149, 1151,1153, 1155, 1157, 1159, 1161, 1163, 1165, 1167, 1169, 1171, 1173, 1175,1177, 1179, 1181, 1183, 1185, 1187, 1189, 1191, 1193, 1195, 1197, 1199,1201, 1203, 1205, 1207, 1209, 1211, 1213, 1215, 1217, 1219, 1221, 1223,1225, 1227, 1229, 1231, 1233, 1235, 1237, 1239, 1241, 1243, 1245, 1247,1249, 1251, 1253, 1255, 1257, 1259, 1261, 1263, 1265, 1267, 1269, 1271,1273, 1275, 1277, 1279, 1281, 1283, 1285, 1287, 1289, 1291, 1293, 1295,1297, 1299, 1301, 1303, 1305, 1307, 1309, 1311, 1313, 1315, 1317, 1319,1321, 1323, 1325, 1327, 1329, 1331, 1333, 1335, 1337, 1339, 1341, 1343,1345, 1347, 1349, 1351, 1353, 1355, 1357, 1359, 1361, 1363, 1365, 1367,1369, 1371, 1373, 1375, 1377, 1379, 1381, 1383, 1385, 1387, 1389, 1391,1393, 1395, 1397, 1399, 1401, 1403, 1405, 1407, 1409, 1411, 1413, 1415,1417, 1419, 1421, 1423, 1425, 1427, 1429, 1431, 1433, 1435, 1437, 1439,1441, 1443, 1445, 1447, 1449, 1451, 1453, 1455, 1457, 1459, 1461, 1463,1465, 1467, 1469, 1471, 1473, 1475, 1477, 1479, 1481, 1483, 1485, 1487,1489, 1491, 1493, 1495, 1497, 1499, 1501, 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6215,6217, 6219, 6221, 6223, 6225, 6227, 6229, 6231, 6233, 6235, 6237, 6239,6241, 6243, 6245, 6247, 6249, 6251, 6253, 6255, 6257, 6259, 6261, 6263,6265, 6267, 6269, 6271, 6273, 6275, 6277, 6279, 6281, 6283, 6285, 6287,6289, 6291, 6293, 6295, 6297, 6299, 6301, 6303, 6305, 6307, 6309, 6311,6313, 6315, 6317, 6319, 6321, 6323, 6325, 6327, 6329, 6331, 6333, 6335,6337, 6339, 6341, 6343, 6345, 6347, 6349, 6351, 6353, 6355, 6357, 6359,6361, 6363, 6365, 6367, 6369, 6371, 6373, 6375, 6377, 6379, 6381, 6383,6385, 6387, 6389, 6391, 6393, 6395, 6397, 6399, 6401, 6403, 6405, 6407,6409, 6411, 6413, 6415, 6417, 6419, 6421, 6423, 6425, 6427, 6429, 6431,6433, 6435, 6437, 6439, 6441, 6443, 6445, 6447, 6449, 6451, 6453, 6455,6457, 6459, 6461, 6463, 6465, 6467, 6469, 6471, 6473, 6475, 6477, 6479,6481, 6483, 6485, 6487, 6489, 6491, 6493, 6495, 6497, 6499, 6501, 6503,6505, 6507, 6509, 6511, 6513, 6515, 6517, 6519, 6521, 6523, 6525, 6527,6529, 6531, 6533, 6535, 6537, 6539, 6541, 6543, 6545, 6547, 6549, 6551,6553, 6555, 6557, 6559, 6561, 6563, 6565, 6567, 6569, 6571, 6573, 6575,6577, 6579, 6581, 6583, 6585, 6587, 6589, 6591, 6593, 6595, 6597, 6599,6601, 6603, 6605, 6607, 6609, 6611, 6613, 6615, 6617, 6619, 6621, 6623,6625, 6627, 6629, 6631, 6633, 6635, 6637, 6639, 6641, 6643, 6645, 6647,6649, 6651, 6653, 6655, 6657, 6659, 6661, 6663, 6665, 6667, 6669, 6671,6673, 6675, 6677, 6679, 6681, 6683, 6685, 6687, 6689, 6691, 6693, 6695,6697, 6699, 6701, 6703, 6705, 6707, 6709, 6711, 6713, 6715, 6717, 6719,6721, 6723, 6725, 6727, 6729, 6731, 6733, 6735, 6737, 6739, 6741, 6743,6745, 6747, 6749, 6751, 6753, 6755, 6757, 6759, 6761, 6763, 6765, 6767,6769, 6771, 6773, 6775, 6777, 6779, 6781, 6783, 6785, 6787, 6789, 6791,6793, 6795, 6797, 6799, 6801, 6803, 6805, 6807, 6809, 6811, 6813, 6815,6817, 6819, 6821, 6823, 6825, 6827, 6829, 6831, 6833, 6835, 6837, 6839,6841, 6843, 6845, 6847, 6849, 6851, 6853, 6855, 6857, 6859, 6861, 6863,6865, 6867, 6869, 6871, 6873, 6875, 6877, 6879, 6881, 6883, 6885, 6887,6889, 6891, 6893, 6895, 6897, 6899, 6901, 6903, 6905, 6907, 6909, 6911,6913, 6915, 6917, 6919, 6921, 6923, 6925, 6927, 6929, 6931, 6933, 6935,6937, 6939, 6941, 6943, 6945, 6947, 6949, 6951, 6953, 6955, 6957, 6959,6961, 6963, 6965, 6967, 6969, 6971, 6973, 6975, 6977, 6979, 6981, 6983,6985, 6987, 6989, 6991, 6993, 6995, 6997, 6999, 7001, 7003, 7005, 7007,7009, 7011, 7013, 7015, 7017, 7019, 7021, 7023, 7025, 7027, 7029, 7031,7033, 7035, 7037, 7039, 7041, 7043, 7045, 7047, 7049, 7051, 7053, 7055,7057, 7059, 7061, 7063, 7065, 7067, 7069, 7071, 7073, 7075, 7077, 7079,7081, 7083, 7085, 7087, 7089, 7091, 7093, 7095, 7097, 7099, 7101, 7103,7105, 7107, 7109, 7111, 7113, 7115, 7117, 7119, 7121, 7123, 7125, 7127,7129, 7131, 7133, 7135, 7137, 7139, 7141, 7143, 7145, 7147, 7149, 7151,7153, 7155, 7157, 7159, 7161, 7163, 7165, 7167, 7169, 7171, 7173, 7175,7177, 7179, 7181, 7183, 7185, 7187, 7189, 7191, 7193, 7195, 7197, 7199,7201, 7203, 7205, 7207, 7209, 7211, 7213, 7215, 7217, 7219, 7221, 7223,7225, 7227, 7229, 7231, 7233, 7235, 7237, 7239, 7241, 7243, 7245, 7247,7249, 7251, 7253, 7255, 7257, 7259, 7261, 7263, 7265, 7267, 7269, 7271,7273, 7275, 7277, 7279, 7281, 7283, 7285, 7287, 7289, 7291, 7293, 7295,7297, 7299, 7301, 7303, 7305, 7307, 7309, 7311, 7313, 7315, 7317, 7319,7321, 7323, 7325, 7327, 7329, 7331, 7333, 7335, 7337, 7339, 7341, 7343,7345, 7347, 7349, 7351, 7353, 7355, 7357, 7359, 7361, 7363, 7365, 7367,7369, 7371, 7373, 7375, 7377, 7379, 7381, 7383, 7385, 7387, 7389, 7391,7393, 7395, 7397, 7399, 7401, 7403, 7405, 7407, 7409, 7411, 7413, 7415,7417, 7419, 7421, 7423, 7425, 7427, 7429, 7431, 7433, 7435, 7437, 7439,7441, 7443, 7445, 7447, 7449, 7451, 7453, 7455, 7457, 7459, 7461, 7463,7465, 7467, 7469, 7471, 7473, 7475, 7477, 7479, 7481, 7483, 7485, 7487,7489, 7491, 7493, 7495, 7497, 7499, 7501, 7503, 7505, 7507, 7509, 7511,7513, 7515, 7517, 7519, 7521, 7523, 7525, 7527, 7529, 7531, 7533, 7535,7537, 7539, 7541, 7543, 7545, 7547, 7549, 7551, 7553, 7555, 7557, 7559,7561, 7563, 7565, 7567, 7569, 7571, 7573, 7575, 7577, 7579, 7581, 7583,7585, 7587, 7589, 7591, 7593, 7595, 7597, 7599, 7601, 7603, 7605, 7607,7609, 7611, 7613, 7615, 7617, 7619, 7621, 7623, 7625, 7627, 7629, 7631,7633, 7635, 7637, 7639, 7641, 7643, 7645, 7647, 7649, 7651, 7653, 7655,7657, 7659, 7661, 7663, 7665, 7667, 7669, 7671, 7673, 7675, 7677, 7679,7681, 7683, 7685, 7687, 7689, 7691, 7693, 7695, 7697, 7699, 7701, 7703,7705, 7707, 7709, 7711, 7713, 7715, 7717, 7719, 7721, 7723, 7725, 7727,7729, 7731, 7733, 7735, 7737, 7739, 7741, 7743, 7745, 7747, 7749, 7751,7753, 7755, 7757, 7759, 7761, 7763, 7765, 7767, 7769, 7771, 7773, 7775,7777, 7779, 7781, 7783, 7785, 7787, 7789, 7791, 7793, 7795, 7797, 7799,7801, 7803, 7805, 7807, 7809, 7811, 7813, 7815, 7817, 7819, 7821, 7823,7825, 7827, 7829, 7831, 7833, 7835, 7837, 7839, 7841, 7843, 7845, 7847,7849, 7851, 7853, 7855, 7857, 7859, 7861, 7863, 7865, 7867, 7869, 7871,7873, 7875, 7877, 7879, 7881, 7883, 7885, 7887, 7889, 7891, 7893, 7895,7897, 7899, 7901, 7903, 7905, 7907, 7909, 7911, 7913, 7915, 7917, 7919,7921, 7923, 7925, 7927, 7929, 7931, 7933, 7935, 7937, 7939, 7941, 7943,7945, 7947, 7949, 7951, 7953, 7955, 7957, 7959, 7961, 7963, 7965, 7967,7969, 7971, 7973, 7975, 7977, 7979, 7981, 7983, 7985, 7987, 7989, 7991,7993, 7995, 7997, 7999, 8001, 8003, 8005, 8007, 8009, 8011, 8013, 8015,8017, 8019, 8021, 8023, 8025, 8027, 8029, 8031, 8033, 8035, 8037, 8039,8041, 8043, 8045, 8047, 8049, 8051, 8053, 8055, 8057, 8059, 8061, 8063,8065, 8067, 8069, 8071, 8073, 8075, 8077, 8079, 8081, 8083, 8085, 8087,8089, 8091, 8093, 8095, 8097, 8099, 8101, 8103, 8105, 8107, 8109, 8111,8113, 8115, 8117, 8119, 8121, 8123, 8125, 8127, 8129, 8131, 8133, 8135,8137, 8139, 8141, 8143, 8145, 8147, 8149, 8151, 8153, 8155, 8157, 8159,8161, 8163, 8165, 8167, 8169, 8171, 8173, 8175, 8177, 8179, 8181, 8183,8185, 8187, 8189, 8191, 8193, 8195, 8197, 8199, 8201, 8203, 8205, 8207,8209, 8211, 8213, 8215, 8217, 8219, 8221, 8223, 8225, 8227, 8229, 8231,8233, 8235, 8237, 8239, 8241, 8243, 8245, 8247, 8249, 8251, 8253, 8255,8257, 8259, 8261, 8263, 8265, 8267, 8269, 8271, 8273, 8275, 8277, 8279,8281, 8283, 8285, 8287, 8289, 8291, 8293, 8295, 8297, 8299, 8301, 8303,8305, 8307, 8309, 8311, 8313, 8315, 8317, 8319, 8321, 8323, 8325, 8327,8329, 8331, 8333, 8335, 8337, 8339, 8341, 8343, 8345, 8347, 8349, 8351,8353, 8355, 8357, 8359, 8361, 8363, 8365, 8367, 8369, 8371, 8373, 8375,8377, 8379, 8381, 8383, 8385, 8387, 8389, 8391, 8393, 8395, 8397, 8399,8401, 8403, 8405, 8407, 8409, 8411, 8413, 8415, 8417, 8419, 8421, 8423,8425, 8427, 8429, 8431, 8433, 8435, 8437, 8439, 8441, 8443, 8445, 8447,8449, 8451, 8453, 8455, 8457, 8459, 8461, 8463, 8465, 8467, 8469, 8471,8473, 8475, 8477, 8479, 8481, 8483, 8485, 8487, 8489, 8491, 8493, 8495,8497, 8499, 8501, 8503, 8505, 8507, 8509, 8511, 8513, 8515, 8517, 8519,8521, 8523, 8525, 8527, 8529, 8531, 8533, 8535, 8537, 8539, 8541, 8543,8545, 8547, 8549, 8551, 8553, 8555, 8557, 8559, 8561, 8563, 8565, 8567,8569, 8571, 8573, 8575, 8577, 8579, 8581, 8583, 8585, 8587, 8589, 8591,8593, 8595, 8597, 8599, 8601, 8603, 8605, 8607, 8609, 8611, 8613, 8615,8617, 8619, 8621, 8623, 8625, 8627, 8629, 8631, 8633, 8635, 8637, 8639,8641, 8643, 8645, 8647, 8649, 8651, 8653, 8655, 8657, 8659, 8661, 8663,8665, 8667, 8669, 8671, 8673, 8675, 8677, 8679, 8681, 8683, 8685, 8687,8689, 8691, 8693, 8695, 8697, 8699, 8701, 8703, 8705, 8707, 8709, 8711,8713, 8715, 8717, 8719, 8721, 8723, 8725, 8727, 8729, 8731, 8733, 8735,8737, 8739, 8741, 8743, 8745, 8747, 8749, 8751, 8753, 8755, 8757, 8759,8761, 8763, 8765, 8767, 8769, 8771, 8773, 8775, 8777, 8779, 8781, 8783,8785, 8787, 8789, 8791, 8793, 8795, 8797, 8799, 8801, 8803, 8805, 8807,8809, 8811, 8813, 8815, 8817, 8819, 8821, 8823, 8825, 8827, 8829, 8831,8833, 8835, 8837, 8839, 8841, 8843, 8845, 8847, 8849, 8851, 8853, 8855,8857, 8859, 8861, 8863, 8865, 8867, 8869, 8871, 8873, 8875, 8877, 8879,8881, 8883, 8885, 8887, 8889, 8891, 8893, 8895, 8897, 8899, 8901, 8903,8905, 8907, 8909, 8911, 8913, 8915, 8917, 8919, 8921, 8923, 8925, 8927,8929, 8931, 8933, 8935, 8937, 8939, 8941, 8943, 8945, 8947, 8949, 8951,8953, 8955, 8957, 8959, 8961, 8963, 8965, 8967, 8969, 8971, 8973, 8975,8977, 8979, 8981, 8983, 8985, 8987, 8989, 8991, 8993, 8995, 8997, 8999,9001, 9003, 9005, 9007, 9009, 9011, 9013, 9015, 9017, 9019, 9021, 9023,9025, 9027, 9029, 9031, 9033, 9035, 9037, 9039, 9041, 9043, 9045, 9047,9049, 9051, 9053, 9055, 9057, 9059, 9061, 9063, 9065, 9067, 9069, 9071,9073, 9075, 9077, 9079, 9081, 9083, 9085, 9087, 9089, 9091, 9093, 9095,9097, 9099, 9101, 9103, 9105, 9107, 9109, 9111, 9113, 9115, 9117, 9119,9121, 9123, 9125, 9127, 9129, 9131, 9133, 9135, 9137, 9139, 9141, 9143,9145, 9147, 9149, 9151, 9153, 9155, 9157, 9159, 9161, 9163, 9165, 9167,9169, 9171, 9173, 9175, 9177, 9179, 9181, 9183, 9185, 9187, 9189, 9191,9193, 9195, 9197, 9199, 9201, 9203, 9205, 9207, 9209, 9211, 9213, 9215,9217, 9219, 9221, 9223, 9225, 9227, 9229, 9231, 9233, 9235, 9237, 9239,9241, 9243, 9245, 9247, 9249, 9251, 9253, 9255, 9257, 9259, 9261, 9263,9265, 9267, 9269, 9271, 9273, 9275, 9277, 9279, 9281, 9283, 9285, 9287,9289, 9291, 9293, 9295, 9297, 9299, 9301, 9303, 9305, 9307, 9309, 9311,9313, 9315, 9317, 9319, 9321, 9323, 9325, 9327, 9329, 9331, 9333, 9335,9337, 9339, 9341, 9343, 9345, 9347, 9349, 9351, 9353, 9355, 9357, 9359,9361, 9363, 9365, 9367, 9369, 9371, 9373, 9375, 9377, 9379, 9381, 9383,9385, 9387, 9389, 9391, 9393, 9395, 9397, 9399, 9401, 9403, 9405, 9407,9409, 9411, 9413, 9415, 9417, 9419, 9421, 9423, 9425, 9427, 9429, 9431,9433, 9435, 9437, 9439, 9441, 9443, 9445, 9447, 9449, 9451, 9453, 9455,9457, 9459, 9461, 9463, 9465, 9467, 9469, 9471, 9473, 9475, 9477, 9479,9481, 9483, 9485, 9487, 9489, 9491, 9493, 9495, 9497, 9499, 9501, 9503,9505, 9507, 9509, 9511, 9513, 9515, 9517, 9519, 9521, 9523, 9525, 9527,9529, 9531, 9533, 9535, 9537, 9539, 9541, 9543, 9545, 9547, 9549, 9551,9553, 9555, 9557, 9559, 9561, 9563, 9565, 9567, 9569, 9571, 9573, 9575,9577, 9579, 9581, 9583, 9585, 9587, 9589, 9591, 9593, 9595, 9597, 9599,9601, 9603, 9605, 9607, 9609, 9611, 9613, 9615, 9617, 9619, 9621, 9623,9625, 9627, 9629, 9631, 9633, 9635, 9637, 9639, 9641, 9643, 9645, 9647,9649, 9651, 9653, 9655, 9657, 9659, 9661, 9663, 9665, 9667, 9669, 9671,9673, 9675, 9677, 9679, 9681, 9683, 9685, 9687, 9689, 9691, 9693, 9695,9697, 9699, 9701, 9703, 9705, 9707, 9709, 9711, 9713, 9715, 9717, 9719,9721, 9723, 9725, 9727, 9729, 9731, 9733, 9735, 9737, 9739, 9741, 9743,9745, 9747, 9749, 9751, 9753, 9755, 9757, 9759, 9761, 9763, 9765, 9767,9769, 9771, 9773, 9775, 9777, 9779, 9781, 9783, 9785, 9787, 9789, 9791,9793, 9795, 9797, 9799, 9801, 9803, 9805, 9807, 9809, 9811, 9813, 9815,9817, 9819, 9821, 9823, 9825, 9827, 9829, 9831, 9833, 9835, 9837, 9839,9841, 9843, 9845, 9847, 9849, 9851, 9853, 9855, 9857, 9859, 9861, 9863,9865, 9867, 9869, 9871, 9873, 9875, 9877, 9879, 9881, 9883, 9885, 9887,9889, 9891, 9893, 9895, 9897, 9899, 9901, 9903, 9905, 9907, 9909, 9911,9913, 9915, 9917, 9919, 9921, 9923, 9925, 9927, 9929, 9931, 9933, 9935,9937, 9939, 9941, 9943, 9945, 9947, 9949, 9951, 9953, 9955, 9957, 9959,9961, 9963, 9965, 9967, 9969, 9971, 9973, 9975, 9977, 9979, 9981, 9983,9985, 9987 and 9989; (b) a nucleotide sequence having at least 80%sequence identity to an amino acid sequence of (a); (c) a nucleotidesequence which is a fragment of at least 10 consecutive nucleotides froma nucleotide sequence of (a); or (d) a nucleotide sequence having atleast 80% sequence identity to a nucleotide sequence of (a) andincluding a fragment of at least 10 consecutive nucleotides from anucleotide sequence of (a).
 5. A pharmaceutical composition comprisingthe polypeptide of claim 2 or claim 3 or the polynucleotide of claim 4,in admixture with a pharmaceutically acceptable carrier.
 6. Apharmaceutical composition comprising two or more polypeptides of claim2 or claim 3, in admixture with a pharmaceutically acceptable carrier.7. The composition of claim 5, further comprising an adjuvant.
 8. Thecomposition of claim 6, further comprising an adjuvant.
 9. A method ofinducing an immune response against an ExPEC bacteria in a subjectcomprising administering to the subject the polypeptide of claim 2 orclaim 3 or the polynucleotide of claim
 4. 10. The method of claim 9,wherein the immune response is a protective immune response.
 11. Amethod of inducing an immune response against an NMEC bacteria in asubject comprising administering to the subject the polypeptide of claim2 or claim 3 or the polynucleotide of claim
 4. 12. The method of claim11, wherein the immune response is a protective immune response.